Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,443,966	C→T	Q306* (CAA→TAA)	ydbH →	putative membrane‑anchored protein, function unknown

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,443,966	0	C	T	100.0%	127.4 / NA	37	Q306* (CAA→TAA)	ydbH	putative membrane‑anchored protein, function unknown
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/22);  total (15/22)

AAGCGATGGTCGCTGGAGCTGGCCGTATGCAGGTTTTCCTTTGAGTGGCCGACTGGGTGTCAAAGTCGACAACTGGCAGGCAGGGCTTGAGAACGCTCTGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGC  >  NC_000913/1443833‑1444096                                                                                                                                      |                                                                                                                                   aaGCGATGGTCGCTGGAGCTGGCCGTATGCAGGTTTTCCTTTGAGTGGCCGACTGGGTGTCAAAGTCGACAACTGGCAGGCAGGGCTTGAGAACGCTCTGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGTAAGCg                                                                                                                               <  2:838788/139‑1 (MQ=255)              tGGAGCTGGCCGTATGCAGGTTTTCCTTTGAGTGGCCGACTGGGTGTCAAAGTCGACAACTGGCAGGCAGGGCTTGAGAACGCTCTGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGTAAGCGGGTAAGGGCAAcg                                                                                                                  <  2:920200/139‑1 (MQ=255)              tGGAGCTGGCCGTATGCAGGTTTTCCTTTGAGTGGCCGACTGGGTGTCAAAGTCGACAACTGGCAGGCAGGGCTTGAGAACGCTCTGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGTAAGCGGGTAAGGGCAAcg                                                                                                                  <  2:634272/139‑1 (MQ=255)              tGGAGCTGGCCGTATGCAGGTTTTCCTTTGAGTGGCCGACTGGGTGTCAAAGTCGACAACTGGCAGGCAGGGCTTGAGAACGCTCTGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGTAAGCGGGTAAGGGCAAcg                                                                                                                  <  2:1163901/139‑1 (MQ=255)                     ggCCGTATGCAGGTTTTCCTTTGAGTGGCCGACTGGGTGTCAAAGTCGACAACTGGCAGGCAGGGCTTGAGAACGCTCTGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGTAAGGGGGTAAGGGCAACGCGGTGCt                                                                                                           <  2:1071042/139‑1 (MQ=255)                          tATGCAGGTTTTCCTTTGAGTGGCCGACTGGGTGTCAAAGTCGACAACTGGCAGGCAGGGCTTGAGAACGCTCTGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGTAAGCGGGTAAGGGCAACGCGGTGCTTAAtt                                                                                                      >  2:255392/1‑139 (MQ=255)                             gCAGGTTTTCCTTTGAGTGGCCGACTGGGTGTCAAAGTCGACAACTGGCAGGCAGGGCTTGAGAACGCTCTGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGTAAGCGGGTAAggg                                                                                                                       <  1:187531/119‑1 (MQ=255)                             gCAGGTTTTCCTTTGAGTGGCCGACTGGGTGTCAAAGTCGACAACTGGCAGGCAGGGCTTGAGAACGCTCTGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGTAAGCGGGTAAggg                                                                                                                       >  2:187531/1‑119 (MQ=255)                                    ttCCTTTGAGTGGCCGACTGGGTGTCAAAGTCGACAACTGGCAGGCAGGGCTTGAGAACGCTCTGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGTAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAgg                                                                                            <  1:255392/139‑1 (MQ=255)                                           gAGTGGCCGACTGGGTGTCAAAGTCGACAACTGGCAGGCAGGGCTTGAGAACGCTCTGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGTAAGCGGGTAAGGGCAACGCGGTg                                                                                                             <  2:590969/115‑1 (MQ=255)                                           gAGTGGCCGACTGGGTGTCAAAGTCGACAACTGGCAGGCAGGGCTTGAGAACGCTCTGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGTAAGCGGGTAAGGGCAACGCGGTg                                                                                                             >  1:590969/1‑115 (MQ=255)                                              tGGCCGACTGGGTGTCAAAGTCGACAACTGGCAGGCAGGGCTTGAGAACGCTCTGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGTAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGc                                                                                  <  2:1067902/139‑1 (MQ=255)                                                gCCGACTGGGTGTCAAAGTCGACAACTGGCAGGCAGGGCTTGAGAACGCTCTGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGTAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCAt                                                                                >  2:396214/1‑139 (MQ=255)                                                 ccGACTGGGTGTCAAAGTCGACAACTGGCAGGCAGGGCTTGAGAACGCTCTGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGTAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATg                                                                               >  2:1132688/1‑139 (MQ=255)                                                       gggTGTCAAAGTCGACAACTGGCAGGCAGGGCTTGAGAACGCTCTGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGTAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAAc                                                                         <  1:1345970/139‑1 (MQ=255)                                                                         cTGGCAGGCAGGGCTTGAGAACGCTCTGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGTAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAg                                                       <  1:598252/139‑1 (MQ=255)                                                                                gcaggGCTTGAGAACGCTCTGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGTAAGCGGGTAAGGGCAACGCGGTGCTTAATTATGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCg                                                <  2:950041/139‑1 (MQ=255)                                                                                 cagtGCTTGAGAACGCTCTGGTCAGCGGACGACTGAGTGTGCTGACGCAGGGGTAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCgg                                               <  1:1239847/139‑1 (MQ=255)                                                                                                 tctGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGTAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCg                               <  1:1114715/139‑1 (MQ=255)                                                                                                  ctGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGTAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCgg                              <  1:279847/139‑1 (MQ=255)                                                                                                   tGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGTAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGaa                                      <  1:656230/130‑1 (MQ=255)                                                                                                   tGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGTAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGaa                                      >  2:656230/1‑130 (MQ=255)                                                                                                          cGGACGACTGAGTGTGCTGACCCAGGGGTAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAg                                  <  1:508540/127‑1 (MQ=255)                                                                                                          cGGACGACTGAGTGTGCTGACCCAGGGGTAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAg                                  >  2:508540/1‑127 (MQ=255)                                                                                                               gacTGAGTGTGCTGACCCAGGGGTAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGAc                            <  1:930734/128‑1 (MQ=255)                                                                                                               gacTGAGTGTGCTGACCCAGGGGTAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGAATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGAc                            >  2:930734/1‑128 (MQ=255)                                                                                                                    aGTGTGCTGACCCAGGGGTAAGCGGGTAAGGGCAACGCGGTGCTT‑ATTTTGGTCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTatat                <  2:867447/134‑1 (MQ=255)                                                                                                                    aGTGTGCTGACCCAGGGGTAAGCGGGTAAGGGCAACGCGGTGCTT‑ATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTatat                >  1:867447/1‑134 (MQ=255)                                                                                                                           tGACCCAGGGGTAAGCGGGTAAGGGCAACGCGGTGCTTAAtt                                                                                                      <  2:212437/42‑1 (MQ=255)                                                                                                                           tGACCCAGGGGTAAGCGGGTAAGGGCAACGCGGTGCTTAAtt                                                                                                      >  1:212437/1‑42 (MQ=255)                                                                                                                           tGACCCAGGGGTAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGAc                            <  1:1058269/116‑1 (MQ=255)                                                                                                                           tGACCCAGGGGTAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGAc                            >  2:1058269/1‑116 (MQ=255)                                                                                                                           tGACCCAGGGGTAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCA‑GGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTGCCt    >  1:945774/1‑139 (MQ=255)                                                                                                                              cccAGGGGTAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTgc  >  2:839284/1‑139 (MQ=255)                                                                                                                              cccAGGGGTAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTgc  >  2:409655/1‑139 (MQ=255)                                                                                                                                  ggggTAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGc                                                      >  1:942391/1‑83 (MQ=255)                                                                                                                                  ggggTAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGc                                                      <  2:942391/83‑1 (MQ=255)                                                                                                                                      |                                                                                                                                   AAGCGATGGTCGCTGGAGCTGGCCGTATGCAGGTTTTCCTTTGAGTGGCCGACTGGGTGTCAAAGTCGACAACTGGCAGGCAGGGCTTGAGAACGCTCTGGTCAGCGGACGACTGAGTGTGCTGACCCAGGGGCAAGCGGGTAAGGGCAACGCGGTGCTTAATTTTGGCCCAGGAAAATTAAGCATGGATAACAGTCAGCTGCCTCTGCAGCTGACCGGTGAAGCGAAACAGGCGGACCTCATTTTATATGCCCGTTTACCTGC  >  NC_000913/1443833‑1444096

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑