| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,756,208 | G→A | A171T (GCT→ACT) | pykF → | pyruvate kinase I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,756,208 | 0 | G | A | 100.0% | 142.3 / NA | 41 | A171T (GCT→ACT) | pykF | pyruvate kinase I |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (21/20); total (21/20) | |||||||||||
TGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCC > NC_000913/1756072‑1756343 | tGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGACt > 1:1030775/1‑139 (MQ=255) aCGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCActgactga < 1:1270980/139‑1 (MQ=255) aCGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCGGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCActgactga < 2:1324063/139‑1 (MQ=255) cGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGACTGaa > 1:517000/1‑139 (MQ=255) gTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGACTGAAAAAGa < 1:732807/139‑1 (MQ=255) tATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGACTGAAAAAGACAAACAGGAc > 2:1457850/1‑139 (MQ=255) tATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGACTGAAAAAGACAAACAGGAc < 2:1030775/139‑1 (MQ=255) aCCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGACTGAAAAAGa < 1:966928/119‑1 (MQ=255) aCCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGACTGAAAAAGACAAACAGGACCTGATCtt < 2:274542/137‑1 (MQ=255) aCCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGACTGAAAAAGACAAACAGGACCTGATCtt > 1:274542/1‑137 (MQ=255) aCCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGACTGAAAAAGACAAACAGGACCTGATCTTTg > 2:807272/1‑139 (MQ=255) aCCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGCGCGAAAACAAAGGTGTGAACCTGCCTGGCATTTCCATTGCTCTGCCAGCACTGACTGAAAgaga > 2:966928/1‑119 (MQ=255) ttGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGACTGAAAAAGACAAAc > 2:1415590/1‑117 (MQ=255) ttGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGACTGAAAAAGACAAAc < 1:1415590/117‑1 (MQ=255) gAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGACTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAAc < 2:1261198/139‑1 (MQ=255) aaCAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGACTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCg < 2:1308426/139‑1 (MQ=255) cAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGACTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTa < 1:708658/139‑1 (MQ=255) aaGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGACTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGa < 1:986788/139‑1 (MQ=255) aTCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGACTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTttg < 1:866938/139‑1 (MQ=255) tGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGACTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTc > 2:472839/1‑139 (MQ=255) cTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGACTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAAc > 1:1140811/1‑112 (MQ=255) cTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGACTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAAc < 2:1140811/112‑1 (MQ=255) cTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGACTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTgctgct > 1:1150316/1‑135 (MQ=255) cTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGACTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTgctgct < 2:1150316/135‑1 (MQ=255) aacaacGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGACTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTa > 2:1258119/1‑139 (MQ=255) cGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGACTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGt > 1:76414/1‑139 (MQ=255) aCCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGACTGAAAAAGACAAACAGGAc > 2:120441/1‑80 (MQ=255) aCCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGACTGAAAAAGACAAACAGGAc < 1:120441/80‑1 (MQ=255) ggCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGACTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAAc > 1:1409262/1‑94 (MQ=255) ggCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGACTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAAc < 2:1409262/94‑1 (MQ=255) ggCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGACTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGt < 1:421489/129‑1 (MQ=255) ggCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGACTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGt > 2:421489/1‑129 (MQ=255) gAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGACTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACg < 1:321750/139‑1 (MQ=255) tgAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGACTGAAAAAGACaaa > 2:471912/1‑53 (MQ=255) tgAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGACTGAAAAAGACaaa < 1:471912/53‑1 (MQ=255) gAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGACTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGt > 1:1403665/1‑139 (MQ=255) gcctgGCGTTTCCATTGCTCTGCCAGCACTGACTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCAc > 1:572218/1‑139 (MQ=255) gcctgGCGTTTCCATTGCTCTGCCAGCACTGACTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCAc > 2:679475/1‑139 (MQ=255) tCCATTGCTCTGCCAGCACTGACTGAAAAAGACAAACAGGAcc < 1:1301716/43‑1 (MQ=255) tCCATTGCTCTGCCAGCACTGACTGAAAAAGACAAACAGGAcc > 2:1301716/1‑43 (MQ=255) ccAGCACTGACTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTCGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGaa < 2:866695/138‑1 (MQ=255) ccAGCACTGACTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGaaa < 1:1258119/139‑1 (MQ=255) ccAGCACTGACTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGaa > 1:866695/1‑138 (MQ=255) ctgactgaAAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATcc < 1:1457850/139‑1 (MQ=255) | TGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCC > NC_000913/1756072‑1756343 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |