| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 93,429 | C→T | S88S (AGC→AGT) | murE → | UDP‑N‑acetylmuramoyl‑L‑alanyl‑D‑glutamate:meso‑ diaminopimelate ligase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 93,429 | 0 | C | T | 100.0% | 117.7 / NA | 34 | S88S (AGC→AGT) | murE | UDP‑N‑acetylmuramoyl‑L‑alanyl‑D‑glutamate:meso‑ diaminopimelate ligase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (18/16); total (18/16) | |||||||||||
TCAGGCGGACGGGCGTCGATATATCCCGCAGGCGATAGCGCAAGGTGTGGCTGCCATTATTGCAGAGGCGAAAGATGAGGCGACCGATGGTGAAATCCGTGAAATGCACGGCGTACCGGTCATCTATCTCAGCCAGCTCAACGAGCGTTTATCTGCACTGGCGGGCCGCTTTTACCATGAACCCTCTGACAATTTACGTCTCGTGGGCGTAACGGGCACCAACGGCAAAACCACGA > NC_000913/93297‑93532 | tcaGGCGGACGGGCGTCGATATATCCCGCAGGCGATAGCGCAAGGTGTGGCTGCCATTATTGCAGAGGCGAAAGATGAGGCGACCGATGGTGAAATCCGTGAAATGCACGGCGTACCGGTCATCTATCTCAGTCAGCTc > 1:1106133/1‑139 (MQ=255)tcaGGCGGACGGGCGTCGATATATCCCGCAGGCGATAGCGCAAGGTGTGGCTGCCATTATTGCAGAGGCGAAAGATGAGGCGACCGATGGTGAAATCCGTGAAATGCACGGCGTACCGGTCATCTATCTCAGTCAGCTc > 1:1340457/1‑139 (MQ=255)tcaGGCGGACGGGCGTCGATATATCCCACAGGCGATAGCGCAAGGTGTGGCTGCCATTATTGCAGAGGCGAAAGATGAGGCGACCGATGGTGAAATCCGTGAAATGCACGGCGTACCGGTCATCTATCTCAGTCAGCTc > 2:646796/1‑139 (MQ=255) ggACGGGCGTCGATATATCCCGCAGGCGATAGCGCAAGGTGTGGCTGCCATTATTGCAGAGGCGAAAGATGAGGCGACCGATGGTGAAATCCGTGAAATGCACGGCGTACCGGTCATCTATCTCAGTCAGCTCAACGAg < 2:1340457/139‑1 (MQ=255) ggACGGGCGTCGATATATCCCGCAGGCGATAGCGCAAGGTGTGGCTGCCATTATTGCAGAGGCGAAAGATGAGGCGACCGATGGTGAAATCCGTGAAATGCACGGCGTACCGGTCATCTATCTCAGTCAGCTCAACGAg < 1:1191525/139‑1 (MQ=255) gACGGGCGTCGATATATCCCGCAGGCGATAGCGCAAGGTGTGGCTGCCATTATTGCAGAGGCGAAAGATGAGGCGACCGATGGTGAAATCCGTGAAATGCACGGCGTACCGGTCATCTATCTCAGTCAGCTCAACGAGc > 2:299357/1‑139 (MQ=255) aCGGGCGTCGATATATCCCGCAGGCGATAGCGCAAGGTGTGGCTGCCATTATTGCAGAGGCGAAAGATGAGGCGACCGATGGTGAAATCCGTGAAATGCACGGCGTACCGGTCATCTATCTCAGTCAGCTCAACGAGCg < 1:299357/139‑1 (MQ=255) cGCAGGCGATAGCGCAAGGTGTGGCTGCCATTATTGCAGAGGCGAAAGATGAGGCGACCGATGGTGAAATCCGTGAAATGCACGGCGTACCGGTCATCTATCTCAGTCAGCTCAACGAGCGTTTATCTGCACTGGCggg > 2:780261/1‑139 (MQ=255) cAGGCGATAGCGCAAGGTGTGGCTGCCATTATTGCAGAGGCGAAAGATGAGGCGACCGATGGTGAAATCCGTGAAATGCACGGCGTACCGGTCATCTATCTCAGTCAGCTCAACGAGCGTTTATCTGCACTGGCGGGcc > 2:1298385/1‑139 (MQ=255) cGATAGCGCAAGGTGTGGCTGCCATTATTGCAGAGGCGAAAGATGAGGCGACCGATGGTGAAATCCGTGAAATGCACGGCGTACCGGTCATCTATCTCAGTCAGCTCAACGAGCGTTTATCTGCACTGGCGGGCCGCtt < 1:1298385/139‑1 (MQ=255) cAAGGTGTGGCTGCCATTATTGCAGAGGCGAAAGATGAGGCGACCGATGGTGAAATCCGTGAAATGCACGGCGTACCGGTCATCTATCTCAGTCAGCTCAACGAGCGTTTATCTGCACTGGCGGGCCGCTTTTACCATg < 2:294777/139‑1 (MQ=255) aaGGTGTGGCTGCCATTATTGCAGAGGCGAAAGATGAGGCGACCGATGGTGAAATCCGTGAAATGCACGGCGTACCGGTCATCTATCTCAGTCAGCTCAACGAGCGTTTATCTGCACTGGCGGGc > 1:412373/1‑125 (MQ=255) aaGGTGTGGCTGCCATTATTGCAGAGGCGAAAGATGAGGCGACCGATGGTGAAATCCGTGAAATGCACGGCGTACCGGTCATCTATCTCAGTCAGCTCAACGAGCGTTTATCTGCACTGGCGGGc < 2:412373/125‑1 (MQ=255) aGGTGTGGCTGCCATTATTGCAGAGGCGAAAGATGAGGCGACCGATGGTGAAATCCGTGAAATGCACGGCGTACCGGTCATCTATCTCAGTCAGCTCAACGAGCGTTTATCTGCACTGGCGGGCCGCTTTTACCATGaa < 1:880992/139‑1 (MQ=255) gCTGCCATTATTGCAGAGGCGAAAGATGAGGCGACCGATGGTGAAATCCGTGAAATGCACGGCGTACCGGTCATCTATCTCAGTCAGCTCAACGAGCGTTTATCTGCACTGGCGGGCCGCTTTTACCATGAACCCTCTg < 2:1106133/139‑1 (MQ=255) tGCCATTATTGCAGAGGCGAAAGATGAGGCGACCGATGGTGAAATCCGTGAAATGCACGGCGTACCGGTCATCTATCTCAGTCAGCTCAACGAGCGTTTATCTGCACTGGCGGGCCGCTTTTACCATGAACCCTCTGAc < 2:1307978/139‑1 (MQ=255) agagGCGAAAGATGAGGCGACCGATGGTGAAATCCGTGAAATGCACGGCGTACCGGTCATCTATCTCAGTCAGCTCAACGAGCGTTTATCTGCACTGGCGGGCCGCTTTTACGATGAACCCTCTGACAATTTACGtctc > 2:933603/1‑139 (MQ=255) gCGAAAGATGAGGCGACCGATGGTGAAATCCGTGAAATGCACGGCGTACCGGTCATCTATCTCAGTCAGCTCAACGAGCGTTTATCTGCACTGGCGGGCCGCTTTTACCATGAACCCTCTGACAATTTACGTCCCGTgg > 2:8577/1‑139 (MQ=255) aGGCGACCGATGGTGAAATCCGTGAAATGCACGGCGTACCGGTCATCTATCTCAGTCAGCTCAACGAGCGTTTATCTGCACTGGCGGGCCGCTTTTACCATg < 1:801035/102‑1 (MQ=255) aGGCGACCGATGGTGAAATCCGTGAAATGCACGGCGTACCGGTCATCTATCTCAGTCAGCTCAACGAGCGTTTATCTGCACTGGCGGGCCGCTTTTACCATg > 2:801035/1‑102 (MQ=255) aGGCGACCGATGGTGAAATCCGTGAAATGCACGGCGTACCGGTCATCTATCTCAGTCAGCTCAACGAGCGTTTATCTGCACTGGCGGGCCGCTTTTACCATGAACCCTTTGACAATTTACGTCTCGTGGGCGTAACggg > 2:1359217/1‑139 (MQ=255) aCCGATGGTGAAATCCGTGAAATGCACGGCGTACCGGTCATCTATCTCAGTCAGCTCAACGAGCGTTTATCTGCACTGGCGGGCCGCtt > 2:937646/1‑89 (MQ=255) aCCGATGGTGAAATCCGTGAAATGCACGGCGTACCGGTCATCTATCTCAGTCAGCTCAACGAGCGTTTATCTGCACTGGCGGGCCGCtt < 1:937646/89‑1 (MQ=255) aCCGATGGTGAAATCCGTGAAATGCACGGCGTACCGGTCATCTATCTCAGTCAGCTCAACGAGCGTTTATCTGCACTGGCGGGCCGCTTTTACCATGAACCCTCTGACAATTTACGTCTCGTGGGCGTAACGGGCACCa > 2:1464053/1‑139 (MQ=255) tGGTGAAATCCGTGAAATGCACGGCGTACCGGTCATCTATCTCAGTCAGCTCAACGAGCGTTTATCTGCACTGGCGGGCCGCTTTTACCATGAACCCTCTGACAATTTACGTCTCGTGGGCGTAACGGGCACCAACGGc < 1:780261/139‑1 (MQ=255) cGTGAAATGCACGGCGTACCGGTCATCTATCTCAGTCAGCTCAACGAGCGTTTATCTGCACTGGCGGGCCGCTTTTACCATGAACCCTCTGACAATTTACGTCTCGTGGGCGTAACGGGCACCAACGGCAAAACCACGa > 2:100804/1‑139 (MQ=255) cGTGAAATGCACGGCGTACCGGTCATCTATCTCAGTCAGCTCAACGAGCGTTTATCTGCACTGGCGGGCCGCTTTTACCATGAACCCTCTGACAATTTACGTCTCGTGGGCGTAACGGGCACCAACGGCAAAACCACGa < 1:284544/139‑1 (MQ=255) cGTGAAATGCACGGCGTACCGGTCATCTATCTCAGTCAGCTCAACGAGCGTTTATCTGCACTGGCCGGCCGCTTTTACCATGAACCCTCTGACAATTTACGTCTCGTGGGCGTAACGGGCACCAACGGCAAAACCACGa > 2:179967/1‑139 (MQ=255) gTGAAATGCACGGCGTACCGGTCATCTATCTCAGTCAGCTCAACGAGCg < 2:1185187/49‑1 (MQ=255) gTGAAATGCACGGCGTACCGGTCATCTATCTCAGTCAGCTCAACGAGCg > 1:1185187/1‑49 (MQ=255) tGAAATGCACGGCGTACCGGTCATCTATCTCAGTCAGCTCAACGAGCGttt > 1:812175/1‑51 (MQ=255) tGAAATGCACGGCGTACCGGTCATCTATCTCAGTCAGCTCAACGAGCGttt < 2:812175/51‑1 (MQ=255) atctatctCAGTCAGCTCAACGAGCGTTTATCTGCACTGGCGGGCCGCTTTTACCATGAACCct > 2:1320932/1‑64 (MQ=255) atctatctCAGTCAGCTCAACGAGCGTTTATCTGCACTGGCGGGCCGCTTTTACCATGAACCct < 1:1320932/64‑1 (MQ=255) cagtcagcTCAACGAGCGTTTATCTGCACTGGCGGGc < 2:1045404/37‑1 (MQ=255) cagtcagcTCAACGAGCGTTTATCTGCACTGGCGGGc > 1:1045404/1‑37 (MQ=255) | TCAGGCGGACGGGCGTCGATATATCCCGCAGGCGATAGCGCAAGGTGTGGCTGCCATTATTGCAGAGGCGAAAGATGAGGCGACCGATGGTGAAATCCGTGAAATGCACGGCGTACCGGTCATCTATCTCAGCCAGCTCAACGAGCGTTTATCTGCACTGGCGGGCCGCTTTTACCATGAACCCTCTGACAATTTACGTCTCGTGGGCGTAACGGGCACCAACGGCAAAACCACGA > NC_000913/93297‑93532 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |