Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 2,268,372 | G→A | E182K (GAG→AAG) | yeiR → | Zn‑stimulated GTPase involved in zinc homeostasis, mutants are cadmium and EDTA sensitive, Zn(2+) binding protein |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,268,372 | 0 | G | A | 100.0% | 153.1 / NA | 44 | E182K (GAG→AAG) | yeiR | Zn‑stimulated GTPase involved in zinc homeostasis, mutants are cadmium and EDTA sensitive, Zn(2+) binding protein |
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (20/24); total (20/24) |
AAAACTTCCGTGACCAGCTGGCTGCCGCAGACATCATTGTCGCCAATAAATCCGACCGTACGACGCCCGAAAGTGAGCAAGCGCTACAGCGTTGGTGGCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAG > NC_000913/2268238‑2268498 | aaaaCTTCCGTGACCAGCTGGCTGCCGCAGACATCATTGTCGCCAATAAATCCGACCGTACGACGCCCGAAAGTGAGCAAGCGCTACAGCGTTGGTGGCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTAAGCa < 2:475546/139‑1 (MQ=255) cAGCTGGCTGCCGCAGACATCATTGTCGCCAATAAATCCGACTGTACGACGCCCGAAAGTGAGCAAGCGCTACAGCGTTGGTGGCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTAAGCATGGGAAAGTTGACg < 1:1189283/139‑1 (MQ=255) cAGCTGGCTGCCGCAGACATCATTGTCGCCAATAAATCCGACCGTACGACGCCCGAAAGTGAGCAAGCGCTACAGCGTTGGTGGCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTAAGCATGGGAAAGTTGACg > 1:595069/1‑139 (MQ=255) aGCTGGCTGCCGCAGACATCATTGTCGCCAATAAATCCGACCGTACGACGCCCGAAAGTGAGCAAGCGCTACAGCGTTGGTGGCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTAAGCATGGGAAAGTTGACgg > 2:21800/1‑139 (MQ=255) tgCCGCAGACATCATTGTCGCCAATAAATCCGACCGTACGACGCCCGAAAGTGAGCAAGCGCTACAGCGTTGGTGGCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTAAGCATGGGAAAGTTGACGGTCAtctt > 1:759551/1‑139 (MQ=255) aGACATCATTGTCGCCAATAAATCCGACCGTACGACGCCCGAAAGTGAGCAAGCGCTACAGCGTTGGTGGCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTAAGCATGGGAAAGTTGACGGTCATCTTCTGGAt < 1:1109877/139‑1 (MQ=255) atcatTGTCGCCAATAAATCCGACCGTACGACGCCCGAAAGTGAGCAAGCGCTACAGCGTTGGTGGCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTAAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGc < 1:191862/139‑1 (MQ=255) atcatTGTCGCCAATAAATCCGACCGTACGACGCCCGAAAGTGAGCAAGCGCTACAGCGTTGGTGGCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTAAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGc < 2:764376/139‑1 (MQ=255) tGTCGCCAATAAATCCGACCGTCCGACGCCCGAAAGTGAGCAAGAGCTACAGCGTTGGTGGCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTAAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGt > 1:1396922/1‑139 (MQ=255) tGTCGCCAATAAATCCGACCGTACGACGCCCGAAAGTGAGCAAGCGCTACAGCGTTGGTGGCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTAAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGt < 1:592962/139‑1 (MQ=255) aaTAAATCCGACCGTACGACGCCCGAAAGTGAGCAAGCGCTACAGCGTTGGTGGCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTAAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAAtt > 1:1351194/1‑139 (MQ=255) aCCGTACGACGCCCGAAAGTGAGCAAGCGCTACAGCGTTGGTGGCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTAAGCATGGGAAAGTTGACGGTc < 1:578728/102‑1 (MQ=255) aCCGTACGACGCCCGAAAGTGAGCAAGCGCTACAGCGTTGGTGGCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTAAGCATGGGAAAGTTGACGGTc > 2:578728/1‑102 (MQ=255) tACGACGCCCGAAAGTGAGCAAGCGCTACAGCGTTGGTGGCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTAAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGccc > 2:686795/1‑139 (MQ=255) tACGACGCCCGAAAGTGAGCAAGCGCTACAGCGTTGGTGGCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTAAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGccc > 1:563370/1‑139 (MQ=255) cgacgCCCGAAAGTGAGCAAGCGCTACAGCGTTGGTGGCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTAAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGc > 1:1354987/1‑139 (MQ=255) aaaGTGAGCAAGCGCTACAGCGTTGGTGGCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTAAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGcgccgc < 2:1351194/139‑1 (MQ=255) aaaGTGAGCAAGCGCTACAGCGTTGGTGGCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTAAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGcgccgc > 1:1103970/1‑139 (MQ=255) gTGAGCAAGCGCTACAGCGTTGGTGGCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTAAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCa < 2:81500/139‑1 (MQ=255) tGAGCGAGCGCTACAGCGTTGGTGGCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTAAGCATGGGTAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCAt < 1:911006/139‑1 (MQ=255) gCAAGCGCTACAGCGTTGGTGGCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTAAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATtct < 1:211062/139‑1 (MQ=255) aaGCGCTACAGCGTTGGTGGCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTAAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCtca < 2:1396922/139‑1 (MQ=255) aCAGCGTTGGTGGCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTAAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCcg > 1:1033181/1‑88 (MQ=255) aCAGCGTTGGTGGCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTAAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCcg < 2:1033181/88‑1 (MQ=255) tggtggCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTAAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCAtt > 1:371710/1‑121 (MQ=255) tggtggCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTAAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCAtt < 2:371710/121‑1 (MQ=255) tggtggCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTAAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGa > 1:1019793/1‑139 (MQ=255) gtggCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTAAGCATGGGAAAGTTGACGGt > 2:741687/1‑61 (MQ=255) gtggCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTAAGCATGGGAAAGTTGACGGt < 1:741687/61‑1 (MQ=255) ggCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTAAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGaaaaa < 2:687248/139‑1 (MQ=255) ggCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTAAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGaaaaa < 2:300109/139‑1 (MQ=255) agcaAAATGGTGGCGATCGACAATTAATTCACAGTAAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCa < 2:230316/112‑1 (MQ=255) agcaAAATGGTGGCGATCGACAATTAATTCACAGTAAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCa > 1:230316/1‑112 (MQ=255) aaTGGTGGCGATCGACAATTAATTCACAGTAAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTag < 2:803443/139‑1 (MQ=255) gCGATCGACAATTAATTCACAGTAAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGtt > 2:1399145/1‑139 (MQ=255) aTCGACAATTAATTCACAGTAAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTg < 2:878307/80‑1 (MQ=255) aTCGACAATTAATTCACAGTAAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTg > 1:878307/1‑80 (MQ=255) tCGACAATTAATTCACAGTAAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGc < 1:686795/139‑1 (MQ=255) attaattCACAGTAAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCa > 1:65315/1‑139 (MQ=255) ttaattCACAGTAAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCag < 2:759551/139‑1 (MQ=255) caGTAAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCg > 2:1390543/1‑59 (MQ=255) caGTAAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCg < 1:1390543/59‑1 (MQ=255) caGTAAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCAt > 2:246918/1‑82 (MQ=255) caGTAAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCAt < 1:246918/82‑1 (MQ=255) | AAAACTTCCGTGACCAGCTGGCTGCCGCAGACATCATTGTCGCCAATAAATCCGACCGTACGACGCCCGAAAGTGAGCAAGCGCTACAGCGTTGGTGGCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAG > NC_000913/2268238‑2268498 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |