Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,635,578 A→G K449K (AAA→AAG xseA → exonuclease VII, large subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,635,5780AG98.8% 304.0 / NA 81K449K (AAA→AAGxseAexonuclease VII, large subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (41/39);  minor base C (0/1);  total (41/40)
Fisher's exact test for biased strand distribution p-value = 4.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTACTACTGCTACTGACGGCAATGTACTGAAAAAAGTGAAGCAAGTTAAAGCGGGTGAAATGCTAACCACACGTCTGGAAGACGGCTGGATAGAAAGTGAAGTAAAAAACATCCAGCCAGTAAAAAAATCGCGTAAAAAGGTGCATTAAGCCAGCACGAATTCAACGCGTTTTTTCGATATCAAGCCATGCCCGTGCTGACAGAAGTAATCTACTGCACCACAGGCTTTTAACACCTGCAACGGTTGATGGCAG  >  NC_000913/2635454‑2635707
                                                                                                                            |                                                                                                                                 
ttACTACTGCTACTGACGGCAATGTACTGAAAAAAGTGAAGCAAGTTAAAGCGGGTGAAATGCTAACCACACGTCTGGAAGACGGCTGGATAGAAAGTGAAGTAAAAAACATCCAGCCAGTAAAGAAATCGCGTaaaaa                                                                                                                     >  2:1104261/1‑139 (MQ=255)
 tactacTGCTACTGACGGCAATGTACTGAAAAAAGTGAAGCAAGTTAAAGCGGGTGAAATGCTAACCACACGTCTGGAAGACGGCTGGATAGAAAGTGAAGTAAAAAACATCCAGCCAGTAAAGAAATCGCGTaa                                                                                                                        <  2:1151208/135‑1 (MQ=255)
 tactacTGCTACTGACGGCAATGTACTGAAAAAAGTGAAGCAAGTTAAAGCGGGTGAAATGCTAACCACACGTCTGGAAGACGGCTGGATAGAAAGTGAAGTAAAAAACATCCAGCCAGTAAAGAAATCGCGTaa                                                                                                                        >  1:1151208/1‑135 (MQ=255)
  actactGCTACTGACGGCAATGTACTGAAAAAAGTGAAGCAAGTTAAAGCGGGTGAAATGCTAACCACACGTCTGGAAGACGGCTGGATAGAAAGTGAAGTAAAAAACATCCAGCCAGTAAAGAAATCGCGTAAAAAgg                                                                                                                   <  1:790311/139‑1 (MQ=255)
  actactGCTACTGACGGCAATGTACTGAAAAAAGTGAAGCAAGTTAAAGCGGGTGAAATGCTAACCACACGTCTGGAAGACGGCTGGATAGAAAGTGAAGTAAAAAACATCCAGCCAGTAAAGAAATCGCGTAAAAAgg                                                                                                                   >  1:376866/1‑139 (MQ=255)
   ctactgctactgACGGCAATGTACTGAAAAAAGTGAAGCAAGTTAAAGCGGGTGAAATGCTAACCACACGTCTGGAAGACGGCTGGATAGAAAGTGAAGTAAAAAACATCCAGCCAGTAAAGAAATCGCGAAAAAAGGt                                                                                                                  >  2:535046/1‑139 (MQ=255)
        gctactgACGGCAATGTACTGAAAAAAGTGAAGCAAGTTAAAGCGGGTGAAATGCTAACCACACGTCTGGAAGACGGCTGGATAGAAAGTGAAGTAAAAAACATCCAGCCAGTAAAGAAATCGCGTaaaa                                                                                                                      >  1:512723/1‑130 (MQ=255)
        gctactgACGGCAATGTACTGAAAAAAGTGAAGCAAGTTAAAGCGGGTGAAATGCTAACCACACGTCTGGAAGACGGCTGGATAGAAAGTGAAGTAAAAAACATCCAGCCAGTAAAGAAATCGCGTaaaa                                                                                                                      <  2:512723/130‑1 (MQ=255)
        gctactgACGGCAATGTACTGAAAAAAGTGAAGCAAGTTAAAGCGGGTGAAATGCTAACCACACGTCTGGAAGACGGCTGGATAGAAAGTGAAGTAAAAAACATCCAGCCAGTAAAGAAATCGCGTAAAAAGGTGCAtt                                                                                                             >  2:1017402/1‑139 (MQ=255)
           actgACGGCAATGTACTGAAAAAAGTGAAGCAAGTTAAAGCGGGTGAAATGCTAACCACACGTCTGGAAGACGGCTGGATAGAAAGTGAAGTAAAAAACATCCAGCCAGTaaagaaa                                                                                                                                >  1:424369/1‑117 (MQ=255)
           actgACGGCAATGTACTGAAAAAAGTGAAGCAAGTTAAAGCGGGTGAAATGCTAACCACACGTCTGGAAGACGGCTGGATAGAAAGTGAAGTAAAAAACATCCAGCCAGTaaagaaa                                                                                                                                <  2:424369/117‑1 (MQ=255)
           actgACGGCAATGTACTGAAAAAAGTGAAGCAAGTTAAAGCGGGTGAAATGCTAACCACACGTCTGGAAGACGGCTGGATAGAAAGTGAAGTAAAAAACATCCAGCCAGTAAAGAAATCGCGTAAAAAGGTGCATTAAg                                                                                                          >  2:272904/1‑139 (MQ=255)
           actgACGGCAATGTACTGAAAAAAGTGAAGCAAGTTAAAGCGGGTGAAATGCTAACCACACGTCTGGAAGACGGCTGGATAGAAAGTGAAGTAAAAAACATCCAGCCAGTAAAGAAATCGCGTAAAAAGGTGCATTAAg                                                                                                          >  2:283523/1‑139 (MQ=255)
             tgACGGCAATGTACTGAAAAAAGTGAAGCAAGTTAAAGCGGGTGAAATGCTAACCACACGTCTGGAAGACGGCTGGATAGAAAGTGAAGTAAAAAACATCCAGCCAGTAAAGAAATCGCGTAAAAAGGTGCATTAAGcc                                                                                                        <  1:1150513/139‑1 (MQ=255)
                 ggCAATGTACTGAAAAAAGTGAAGCAAGTTAAAGCGGGTGAAATGCTAACCACACGTCTGGAAGACGGCTGGATAGAAAGTGAAGTAAAAAACATCCAGCCAGTAAAGAAATCGCGTAAAAAGGTGCATTAAGCCAGCa                                                                                                    >  2:638538/1‑139 (MQ=255)
                 ggCAATGTACTGAAAAAAGTGAAGCAAGTTAAAGCGGGTGAAATGCTAACCACACGTCTGGAAGACGGCTGGATAGAAAGTGAAGTAAAAAACATCCAGCCAGTAAAGAAATCGCGTAAAAAGGTGCATTAAGCCAGCa                                                                                                    >  1:1174797/1‑139 (MQ=255)
                   cAATGTACTGAAAAAAGTGAAGCAAGTTAAAGCGGGTGAAATGCTAACCACACGTCTGGAAGACGGCTGGATAGAAAGTGAAGTAAAAAACATCCAGCCAGTAAAGAAATCGCGTAAAAAGGTGCATTAAGCCAGCACg                                                                                                  >  1:1188841/1‑139 (MQ=255)
                           tGAAAAAAGTGAAGCAAGTTAAAGCGGGTGAAATGCTAACCACACGTCTGGAAGACGGCTGGATAGAAAGTGAAGTAAAAAACATCCAGCCAGTAAAGAAATCGCGTAAAAAGGTGCATTAAGCCAGCACGAATTCAAc                                                                                          >  1:1275916/1‑139 (MQ=255)
                             aaaaaaGTGAAGCAAGTTAAAGCGGGTGAAATGCTAACCACACGTCTGGAAGACGGCTGGATAGAAAGTGAAGTAAAAAACATCCAGCCAGTAAAGAAATCGCGTAAAAAGGTGCATTAAGCCAGCACGAATTCAAcgc                                                                                        >  2:1462919/1‑139 (MQ=255)
                                aaaGTGAAGCAAGTTAAAGCGGGTGAAATGCTAACCACACGTCTGGAAGACGGCTGGATAGAAAGTGAAGTAAAAAACATCCAGCCAGTAAAGAAATCGCGTAAAAAGGTGCATTAAGCCAGCACGAATTCAACGCGtt                                                                                     >  1:264087/1‑139 (MQ=255)
                                  aGTGAAGCAAGTTAAAGCGGGTGAAATGCTAACCACACGTCTGGAAGACGGCTGGATAGAAAGTGAAGTAAAAAACATCCAGCCAGTAAAGTAATCGCGTAAAAAGGTGCATTAAGCCAGCACGAATTCAACGCGtttt                                                                                   >  1:874862/1‑139 (MQ=255)
                                  aGTGAAGCAAGTTAAAGCGGGTGAAATGCTAACCACACGTCTGGAAGACGGCTGGATAGAAAGTGAAGTAAAAAACATCCAGCCAGTAAAGAAATCGCGTAAAAAGGTGCATTAAGCCAGCACGAATTCAACGCGtttt                                                                                   >  1:980305/1‑139 (MQ=255)
                                  aGTGAAGCAAGTTAAAGCGGGTGAAATGCTAACCACACGTCTGGAAGACGGCTGGATAGAAAGTGAAGTAAAAAACATCCAGCCAGTAAAGAAATCGCGTAAAAAGGTGCATTAAGCCAGCACGAATTCAACGCGtttt                                                                                   >  2:1199780/1‑139 (MQ=255)
                                     gAAGCAAGTTAAAGCGGGTGAAATGCTAACCACACGTCTGGAAGACGGCTGGATAGAAAGTGAAGTAAAAAACATCCAGCCAGTAAAGAAATCGCGTAAAAAGGTGCATTAAGCCAGCACGAATTCAACGCGTTTTTTc                                                                                <  2:13507/139‑1 (MQ=255)
                                     gAAGCAAGTTAAAGCGGGTGAAATGCTAACCACACGTCTGGAAGACGGCTGGATAGAAAGTGAAGTAAAAAACATCCAGCCAGTAAAGAAATCGCGTAAAAAGGTGCATTAAGCCAGCACGAATTCAACGCGTTTTTTc                                                                                <  2:73841/139‑1 (MQ=255)
                                      aaGCAAGTTAAAGCGGGTGAAATGCTAACCACACGTCTGGAAGACGGCTGGATAGAAAGTGAAGTAAAAAACATCCAGCCAGTAAAGAAATCGCGTAAAAAGGTGCATTAAGCCAGCACGAATTCAACGCGTTTTTTCg                                                                               >  2:143745/1‑139 (MQ=255)
                                       aGCAAGTTAAAGCGGGTGAAATGCTAACCACACGTCTGGAAGACGGCTGGATAGAAAGTGAAGTAAAAAACATCCAGCCAGTAAAGAAATCGCGTAAAAAGGTGCATTAAGCCAGCACGAATTCAACGCGtttttt                                                                                 <  2:7726/136‑1 (MQ=255)
                                       aGCAAGTTAAAGCGGGTGAAATGCTAACCACACGTCTGGAAGACGGCTGGATAGAAAGTGAAGTAAAAAACATCCAGCCAGTAAAGAAATCGCGTAAAAAGGTGCATTAAGCCAGCACGAATTCAACGCGtttttt                                                                                 >  1:7726/1‑136 (MQ=255)
                                       aGCAAGTTAAAGCGGGTGAAATGCTAACCACACGTCTGGAAGACGGCTGGATAGAAAGTGAAGTAAAAAACATCCAGCCAGTAAAGAAATCGCGTAAAAAGGTGCATTAAGCCAGCACGAATTCAACGCGTTTTTTCGa                                                                              >  2:775008/1‑139 (MQ=255)
                                          aaGTTATAGCGGGTGAAATGCTAACCACACGTCTGGAAGACGGCTAGATAGAAAGTGAAGTAAAAAACATCCAGCCAGTAAACAAATAGCGTAAAAAGGTGCATTAAGCCAGCACGAATTCAACGCGTTTTTTCGatat                                                                           <  2:1266502/139‑1 (MQ=255)
                                          aaGTTAAAGCGGGTGAAATGCTAACCACACGTCTGGAAGACGGCTGGATAGAAAGTGAAGTAAAAAACATCCAGCCAGTAAAGAAATCGCGTAAAAAGGTGCATTAAGCCAGCACGAATTCAACGCGTTTTTTCGatat                                                                           <  1:272904/139‑1 (MQ=255)
                                          aaGTTAAAGCGGGTGAAATGCTAACCACACGTCTGGAAGACGGCTGGATAGAAAGTGAAGTAAAAAACATCCAGCCAGTAAAGAAATCGCGTAAAAAGGTGCATTAAGCCAGCACGAATTCAACGCGTTTTTTCGatat                                                                           <  1:843744/139‑1 (MQ=255)
                                          aaGTTAAAGCGGGTGAAATGCTAACCACACGTCTGGAAGACGGCTGGATAGAAAGTGAAGTAAAAAACATCCAGCCAGTAAAGAAATCGCGTAAAAAGGTGCATTAAGCCAGCACGAATTCAACGCGTTTTTTCGatat                                                                           <  1:1104261/139‑1 (MQ=255)
                                          aaGTTAAAGCGGGTGAAATGCTAACCACACGTCTGGAAGACGGCTGGATAGAAAGTGAAGTAAAAAACATCCAGCCAGTAAAGAAATCGCGTAAAAAGGTGCATTAAGCCAGCACGAATTCAACGCGTTTTTTCGatat                                                                           <  1:775008/139‑1 (MQ=255)
                                          aaGTTAAAGCGGGTGAAATGCTAACCACACGTCTGGAAGACGGCTGGATAGAAAGTGAAGTAAAAAACATCCAGCCAGTAAAGAAATCGCGTAAAAAGGTGCATTAAGCCAGCACGAATTCAACGCGTTTTTTCGatat                                                                           <  2:282031/139‑1 (MQ=255)
                                          aaGTTAAAGCGGGTGAAATGCTAACCACACGTCTGGAAGACGGCTGGATAGAAAGTGAAGTAAAAAACATCCAGCCAGTAAAGAAATCGCGTAAAAAGGTGCATTAAGCCAGCACGAATTCAACGCGTTTTTTCGatat                                                                           <  1:1434962/139‑1 (MQ=255)
                                           aGTTAAAGCGGGTGAAATGCTAACCACACGTCTGGAAGACGGCTGGATAGAAAGTGAAGTAAAAAACATCCAGCCAGTAAAGAAATCGCGTAAAAAGGTGCATTAAGCCAGCACGAATTCAACGCGTTTTTTCGATATc                                                                          <  1:1462919/139‑1 (MQ=255)
                                               aaaGCGGGTGAAATGCTAACCACACGTCTGGAAGACGGCTGGATAGAAAGTGAAGTAAAAAACATCCAGCCAGTAAAGAAATCGCGTAAAAAGGTGCATTAAGCCAGCACGAATTCAACGCGTTTTTTCGATATCAAGc                                                                      <  1:638538/139‑1 (MQ=255)
                                                 aGCGGGTGAAATGCTAACCACACGTCTGGAAGACGGCTGGATAGAAAGTGACGTAAAAAACATCCAGCCAGTAAAGAAATCGCGTAAAAAGGTGCATTAAGCCAGCACGAATTCAACGCGTTTATTCGATATCAAGCCa                                                                    <  2:980305/139‑1 (MQ=255)
                                                     ggTGAAATGCTAACCACACGTCTGGAAGACGGCTGGATAGAAAGTGAAGTAAAAAACATCCAGCCAGTAAAGAAATCGCGTAAAAAGGTGCATTAAGCCAGCACGAATTCAACGCGTTTTTTCGATATCAAGCCATGcc                                                                >  1:110713/1‑139 (MQ=255)
                                                      gTGAAATGCTAACCACACGTCTGGAAGACGGCTGGATAGAAAGTGAAGTAAAAAACATCCAGCCAGTAAAGAAATCGCGTAAAAAGGTGCATTAAGCCAGCACGAATTCAACGCGTTTTTTCGATATCAAGCCATGccc                                                               >  2:1188239/1‑139 (MQ=255)
                                                      gTGAAATGCTAACCACACGTCTGGAAGACGGCTGGATAGAAAGTGAAGTAAAAAACATCCAGCCAGTAAAGAAATCGCGTAAAAAGGTGCATTAAGCCAGCACGAATTCAACGCGTTTTTTCGATATCAAGCCATGccc                                                               >  1:1137999/1‑139 (MQ=255)
                                                       tGAAATGCTAACCACACGTCTGGAAGACGGCTGGATAGAAAGTGAAGTAAAAAACATCCAGCCAGTAAAGAAATCGCGTAAAAAGGTGCATTAAGCCAGCACGAATTCAACGCGTTTTTTCGATATCAAGCCATGCCCg                                                              >  1:832026/1‑139 (MQ=255)
                                                        gAAATGCTAACCACACGTCTGGAAGACGGCTGGATAGAAAGTGAAGTAAAAAACATCCAGCCAGTAAAGAAATCGCGTAAAAAGGTGCATTAAGCCAGCACGAATTCAACGCGTTTTTTCGATATCAAGCCATGCCCGt                                                             <  2:874862/139‑1 (MQ=255)
                                                         aaaTGCTAACCACACGTCTGGAAGACGGCTGGATAGAAAGTGAAGTAAAAAACATCCAGCCAGTAAAGAAATCGCGTAAAAAGGTGCATTAAGCCAGCACGAATTCAACGCGTTTTTTCGATATCAAGCCATGCCCGTg                                                            <  2:376866/139‑1 (MQ=255)
                                                            tGCTAACCACACGTCTGGAAGACGGCTGGATAGAAAGTGAAGTAAAAAACATCCAGCCAGTAAAGAAATCGCGTAAAAAGGTGCATTAAGCCAGCACGAATTCAACGCGtttttt                                                                                 >  1:961503/1‑115 (MQ=255)
                                                            tGCTAACCACACGTCTGGAAGACGGCTGGATAGAAAGTGAAGTAAAAAACATCCAGCCAGTAAAGAAATCGCGTAAAAAGGTGCATTAAGCCAGCACGAATTCAACGCGtttttt                                                                                 <  2:961503/115‑1 (MQ=255)
                                                             gCTAACCACACGTCTGGAAGACGGCTGGATAGAAAGTGAAGTAAAAAACATCCAGCCAGTAAAGAAATCGCGTAAAAAGGTGCATTAAGCCAGCACGAATTCAACGCGTTTTTTCGATATCAAGCCATGCCCGTGCTGa                                                        <  1:585971/139‑1 (MQ=255)
                                                             gCTAACCACACGTCTGGAAGACGGCTGGATAGAAAGTGAAGTAAAAAACATCCAGCCAGTAAAGAAATCGCGTAAAAAGGTGCATTAAGCCAGCACGAATTCAACGCGTTTTTTCGATATCAAGCCATGCCCGTGCTGa                                                        <  1:283523/139‑1 (MQ=255)
                                                               tAACCACACGTCTGGAAGACGGCTGGATAGAAAGTGAAGTAAAAAACATCCAGCCAGTAAAGAAATCGCGTAAAAAGGTGCATTAAGCCAGCACGAATTCAACGCGTTTTTTCGATATCAAGCCATGCCCGTGCTGACa                                                      >  1:1346715/1‑139 (MQ=255)
                                                                aaCCACACGTCTGGAAGACGGCTGGATAGAAAGTGAAGTAAAAAACATCCAGCCAGTAAAGAAATCGCGTAAAAAGGTGCATTAAGCCAGCACGAATTCAACGCGTTTTTTCGATATCCAGCCATGCCCGTGCTGAc                                                       <  2:813220/137‑1 (MQ=255)
                                                                aaCCACACGTCTGGAAGACGGCTGGATAGAAAGTGAAGTAAAAAACATCCAGCCAGTAAAGAAATCGCGTAAAAAGGTGCATTAAGCCAGCACGAATTCAACGCGTTTTTTCGATATCAAGCCATGCCCGTg                                                            <  1:1206006/132‑1 (MQ=255)
                                                                aaCCACACGTCTGGAAGACGGCTGGATAGAAAGTGAAGTAAAAAACATCCAGCCAGTAAAGAAATCGCGTAAAAAGGTGCATTAAGCCAGCACGAATTCAACGCGTTTTTTCGATATCAAGCCATGCCCGTg                                                            >  2:1206006/1‑132 (MQ=255)
                                                                aaCCACACGTCTGGAAGACGGCTGGATAGAAAGTGAAGTAAAAAACATCCAGCCAGTAAAGAAATCGCGTAAAAAGGTGCATTAAGCCAGCACGAATTCAACGCGTTTTTTCGATATCAAGCCATGCCCGTGCTGAc                                                       >  1:813220/1‑137 (MQ=255)
                                                                aaCCACACGTCTGGAAGACGGCTGGATAGAAAGTGAAGTAAAAAACATCCAGCCAGTAAAGAAATCGCGTAAAAAGGTGCATTAAGCCAGCACGAATTCAACGCGTTTTTTCGATATCAAGCCATGCCCGTGCTGACAg                                                     <  2:1137999/139‑1 (MQ=255)
                                                                   cacaCGTCTGGAAGACGGCTGGATAGAAAGTGAAGTAAAAAACATCCAGCCAGTAAAGAAATCGCGTAAAAAGGTGCATTAAGCCAGCACGAATTCAACGCGTTTTTTCGATATCAAGCCATGCCCGTGCTGACAGAAg                                                  <  1:1145946/139‑1 (MQ=255)
                                                                    acacGTCTGGAAGACGGCTGGATAGAAAGTGAAGTAAAAAACATCCAGCCAGTAAAGAAATCGCGTAAAAAGGTGCATTAAGCCAGCACGAATTCAACGCGTTTTTTCGATATCAAGc                                                                      <  2:11782/118‑1 (MQ=255)
                                                                    acacGTCTGGAAGACGGCTGGATAGAAAGTGAAGTAAAAAACATCCAGCCAGTAAAGAAATCGCGTAAAAAGGTGCATTAAGCCAGCACGAATTCAACGCGTTTTTTCGATATCAAGc                                                                      >  1:11782/1‑118 (MQ=255)
                                                                     cacGTCTGGAAGACGGCTGGATAGAAAGTGAAGTAAAAAACATCCAGCCAGTAAAGAAATCGCGTAAAAAGGTGCATTAAGCCAGCACGAATTCAACGCGTTTTTTCGATATCAAGCCATGCCCGTGCTGACAGAAGTa                                                <  1:1199780/139‑1 (MQ=255)
                                                                      acGTCTGGAAGACGGCTGGATAGAAAGTGAAGTAAAAAACATCCAGCCAGTAAAGAAATCGCGTAAAAAGGTGCATTAAGCCAGCACGAATTCAACGCGTTTTTTCGATATCAAGCCATGCCCGTGCTGACAGAAGTaa                                               >  1:363184/1‑139 (MQ=255)
                                                                        gTCTGGAAGACGGCTGGATAGAAAGTGAAGTAAAAAACATCCAGCCAGTAAAGAAATCGCGTAAAAAGGTGCATTAAGCCAGCACGAATTCAACGCGTTTTTTCGATATCAAGCCATGCCCGTGCTGACAGAAGTAATc                                             >  2:933599/1‑139 (MQ=255)
                                                                           tGGAAGACGGCTGGATAGAAAGTGAAGTAAAAAACATCCAGCCAGTAAAGAAATCGCGTAAAAAGGTGCATTAAGCCAGCACGAATTCAACGCGTTTTTTCGATATCAAGCCATGCCCGTGCt                                                          <  1:944523/123‑1 (MQ=255)
                                                                           tGGAAGACGGCTGGATAGAAAGTGAAGTAAAAAACATCCAGCCAGTAAAGAAATCGCGTAAAAAGGTGCATTAAGACAGCACGAATTCAACGCTTTTTTTCGATATCAAGCCATGCCCGTGCt                                                          >  2:944523/1‑123 (MQ=255)
                                                                                  cGGCTGGATAGAAAGTGAAGTAAAAAACATCCAGCCAGTAAAGAAATCGCGTAAAAAgg                                                                                                                   >  2:1250734/1‑59 (MQ=255)
                                                                                  cGGCTGGATAGAAAGTGAAGTAAAAAACATCCAGCCAGTAAAGAAATCGCGTAAAAAgg                                                                                                                   <  1:1250734/59‑1 (MQ=255)
                                                                                  cGGCTGGATAGAAAGTGAAGTAAAAAACATCCAGCCAGTAAAGAAATCGCGTAAAAAGGTGCATTAAGCCAGCACGAATTCAACGCGTTTTTTCGATATc                                                                          <  2:93056/100‑1 (MQ=255)
                                                                                  cGGCTGGATAGAAAGTGAAGTAAAAAACATCCAGCCAGTAAAGAAATCGCGTAAAAAGGTGCATTAAGCCAGCACGAATTCAACGCGTTTTTTCGATATc                                                                          >  1:93056/1‑100 (MQ=255)
                                                                                   ggCTGGATAGAAAGTGAAGTAAAAAACATCCAGCCAGTAAAGAAATCGCGTAAAAAGGTGCATTAAGCCAGCACGAATTCAACGCGTTTTTTCGATATCAAGCCATGCCCGTGCTGACAGAAGTAATCTACTGcaccac                                  <  2:676663/139‑1 (MQ=255)
                                                                                     cTGGATAGAAAGTGAAGTAAAAAACATCCAGCCAGTAAAGAAATCGCGTAAAAAGGTGCATTAAGCCAGCACGAATTCAACGCGTTTTTTCGATATCAAGCCATGCCCGTGCTGACAGAAGTAATCTACTGCACCACAg                                <  1:252981/139‑1 (MQ=255)
                                                                                      tGGATAGAAAGTGAAGTAAAAAACATCCAGCCAGTAAAGAAATCGCGTAAAAAGGTGCATTAAGCCAGCACGAATTCAACGCGTTTTTTCGATATCAAGCCATGCCCGTGCTGACAGAAGTAATCTACTGCACCACAgg                               <  1:325557/139‑1 (MQ=255)
                                                                                        gATAGAAAGTGAAGTAAAAAACATCCAGCCAGTaaagaaa                                                                                                                                >  1:863336/1‑40 (MQ=255)
                                                                                        gATAGAAAGTGAAGTAAAAAACATCCAGCCAGTaaagaaa                                                                                                                                <  2:863336/40‑1 (MQ=255)
                                                                                             aaaGTGAAGTAAAAAACATCCAGCCAGTAAAGAAATCGCGTAAAAAGGTGCATTAAGCCAGCACGAATTCAACGCGTTTTTTCGATATCAAGCCATGCCCGTGCTGACAGAAGTAATCTACt                                         >  2:1116883/1‑122 (MQ=255)
                                                                                             aaaGTGAAGTAAAAAACATCCAGCCAGTAAAGAAATCGCGTAAAAAGGTGCATTAAGCCAGCACGAATTCAACGCGTTTTTTCGATATCAAGCCATGCCCGTGCTGACAGAAGTAATCTACt                                         <  1:1116883/122‑1 (MQ=255)
                                                                                               aGTGAAGTAAAAAACATCCAGCCAGTAAAGAAATCGCGTAAAAAGGTGCATTAAGCCAGCACGAATTCAACGCGTTTTTTCGatat                                                                           <  1:1428926/86‑1 (MQ=255)
                                                                                               aGTGAAGTAAAAAACATCCAGCCAGTAAAGAAATCGCGTAAAAAGGTGCATTAAGCCAGCACGAATTCAACGCGTTTTTTCGatat                                                                           >  2:1428926/1‑86 (MQ=255)
                                                                                                  gAAGTAAAAAACATCCAGCCAGTAAAGAAATCGCGTAAAAAGGTGCATTAAGCCAGCACGAATTCAACGCGTTTTTTCGATATCAAGCCATGCCCGTGCTGACAGAAGTAATCTACTGCACCACAGGCTTTTAACACCt                   >  1:715521/1‑139 (MQ=255)
                                                                                                   aaGTAAAAAACATCCAGCCAGTAAAGAAATCGCGTAAAAAGGTGCATTAAGCCAGCACGAATTCAACGCGTTTTTTCGATATCAAGCCATGCCCGTGCTGAc                                                       >  2:455575/1‑102 (MQ=255)
                                                                                                   aaGTAAAAAACATCCAGCCAGTAAAGAAATCGCGTAAAAAGGTGCATTAAGCCAGCACGAATTCAACGCGTTTTTTCGATATCAAGCCATGCCCGTGCTGAc                                                       <  1:455575/102‑1 (MQ=255)
                                                                                                    aGTAAAAAACATCCAGCCAGTAAAGAAATCGCGTAAAAAGGTGCATTAAGCCAGCACGAATTCAACGCGTTTTTTCGATATCAAGCCATGCCCGTg                                                            <  1:120463/96‑1 (MQ=255)
                                                                                                    aGTAAAAAACATCCAGCCAGTAAAGAAATCGCGTAAAAAGGTGCATTAAGCCAGCACGAATTCAACGCGTTTTTTCGATATCAAGCCATGCCCGTg                                                            >  2:120463/1‑96 (MQ=255)
                                                                                                           aaCATCCAGCCAGTAAAGAAATCGCGTAAAAAGGTGCATTAAGCCAGCACGAATTCAACGCGTTTTTTCGATATCAAGCCATGCCCGTGCTGACAGAAGTAATCTACTGCACCACAGGCTTTTAACACCTGCAACGGtt          <  1:1310201/139‑1 (MQ=255)
                                                                                                             cATCCAGCCAGTAAAGAAATCGCGTAAAAAGGTGCATTAAGCCAGCACGAATTCAACGCGTTTTTTCGATATCAAGCCATGCCCGTGCTGACAGAAGTAATCTACTGCACCACAGGCTTTTAACACCTGCAACGGTTGa        >  1:908041/1‑139 (MQ=255)
                                                                                                                ccagccagTAAAGAAATCGCGTAAAAAGGTGCATTAAGCCAGCACGAATTCAACGCGTTTTTTCGATATCAAGCCATGCCCGTGCTGACAGAAGTAATCTACTGCACCACAGGCTTTTAACACCTGCAACGGTTGATgg     >  2:917373/1‑139 (MQ=255)
                                                                                                                   gccagTAAAGAAATCGCGTAAAAAGGTGCATTAAGCCAGCACGAATTCAACGCGTTTTTTCGATATCAAGCCATGCCCGTGCTGACAGAAGTAATCTACTGCACCACAGGCTTTTAACACCTGCAACGGTTGATGGCAg  <  2:363184/139‑1 (MQ=255)
                                                                                                                            |                                                                                                                                 
TTACTACTGCTACTGACGGCAATGTACTGAAAAAAGTGAAGCAAGTTAAAGCGGGTGAAATGCTAACCACACGTCTGGAAGACGGCTGGATAGAAAGTGAAGTAAAAAACATCCAGCCAGTAAAAAAATCGCGTAAAAAGGTGCATTAAGCCAGCACGAATTCAACGCGTTTTTTCGATATCAAGCCATGCCCGTGCTGACAGAAGTAATCTACTGCACCACAGGCTTTTAACACCTGCAACGGTTGATGGCAG  >  NC_000913/2635454‑2635707

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: