Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,232,207 T→C S181S (AGT→AGC)  fadH → 2,4‑dienoyl‑CoA reductase, NADH and FMN‑linked

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,232,2070TC100.0% 142.5 / NA 38S181S (AGT→AGC) fadH2,4‑dienoyl‑CoA reductase, NADH and FMN‑linked
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/22);  total (16/22)

TCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCATTATTATCTACCGACTGTCGATGCTCGACCTGGTCGAAGA  >  NC_000913/3232074‑3232335
                                                                                                                                     |                                                                                                                                
tgcTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGCGACCa                                                                                                                             >  2:880346/3‑139 (MQ=255)
 ccTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGCGACCAg                                                                                                                            <  2:98953/139‑1 (MQ=255)
     cAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGCGACCAGTggg                                                                                                                        <  2:680634/139‑1 (MQ=255)
           aTCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGCGACCAGTGGGgcggcg                                                                                                                  >  1:1114125/1‑139 (MQ=255)
           aTCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGCGACCAGTGGGgcggcg                                                                                                                  >  1:193867/1‑139 (MQ=255)
           aTCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGCGACCAGTGGGgcggcg                                                                                                                  >  1:1172837/1‑139 (MQ=255)
                   tttCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGCGACCAGTGGGGCGGCGATTACCGc                                                                                                          <  2:1213626/139‑1 (MQ=255)
                      cGCCCGCTGCGCGCAACTGGCGCGGGAGACAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGCGACCAGTGGGGCGGCGATTACCGCAAc                                                                                                       <  1:880346/139‑1 (MQ=255)
                              gcgcgcAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGCGACCAGTGGGGCGGCGATTACCGCAACCGGATGCg                                                                                               >  1:1197578/1‑139 (MQ=255)
                                    aaCTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGCGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGc                                                                                         <  2:38923/139‑1 (MQ=255)
                                    aaCTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGCGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGc                                                                                         <  2:1114125/139‑1 (MQ=255)
                                      cTGGCGCGGGAGGCAGGATACGACTGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGCGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCg                                                                                       <  1:820772/139‑1 (MQ=255)
                                      cTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGCGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCg                                                                                       <  1:1106998/139‑1 (MQ=255)
                                      cTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGCGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCg                                                                                       <  2:1255266/139‑1 (MQ=255)
                                                     ggATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGCGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAgtgcgtg                                                                        <  1:405451/139‑1 (MQ=255)
                                                                tgtAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGCGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGaa                                                             >  1:787174/1‑139 (MQ=255)
                                                                 gtAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGCGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAAc                                                            <  1:870071/139‑1 (MQ=255)
                                                                          aTGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGCGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGAtt                                                                                            <  2:176445/98‑1 (MQ=255)
                                                                          aTGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGCGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGAtt                                                                                            >  1:176445/1‑98 (MQ=255)
                                                                          aTGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGCGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCa                                                   >  2:236837/1‑139 (MQ=255)
                                                                            gggTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGCGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAAc                                                 <  2:807349/139‑1 (MQ=255)
                                                                               ttCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGCGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGAc                                              <  2:193867/139‑1 (MQ=255)
                                                                                   gAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGCGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCa                                          <  2:1197578/139‑1 (MQ=255)
                                                                                                 cAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGCGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGAttt                                                                                           >  2:893517/1‑76 (MQ=255)
                                                                                                 cAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGCGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGAttt                                                                                           <  1:893517/76‑1 (MQ=255)
                                                                                                 cAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGCGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCa                                          <  1:78051/125‑1 (MQ=255)
                                                                                                 cAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGCGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCa                                          >  2:78051/1‑125 (MQ=255)
                                                                                                 cAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGCGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCATTATTATCTACCGa                            >  2:897117/1‑139 (MQ=255)
                                                                                                   aCGAATTTCTGACGCTGCGCACCAATCAGCGTAGCGACCAGTGGGgcggcg                                                                                                                  >  1:468501/1‑51 (MQ=255)
                                                                                                   aCGAATTTCTGACGCTGCGCACCAATCAGCGTAGCGACCAGTGGGgcggcg                                                                                                                  <  2:468501/51‑1 (MQ=255)
                                                                                                      aaTTTCTGACGCTGCGCACCAATCAGCGTAGCGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCATTATTATCTACCGACTGTc                       <  1:897117/139‑1 (MQ=255)
                                                                                                           cTGACGCTGCGCACCAATCAGCGTAGCGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGAt                                                                                             >  2:1223179/1‑64 (MQ=255)
                                                                                                           cTGACGCTGCGCACCAATCAGCGTAGCGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGAt                                                                                             <  1:1223179/64‑1 (MQ=255)
                                                                                                                  tGCGCACCAATCAGCGTAGCGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCATTATTATCTACCGACTGTCGATGCTCGACCt           >  2:820000/1‑139 (MQ=255)
                                                                                                                  tGCGCACCAATCAGCGTAGCGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCATTATTATCTACCGACTGTCGATGCTCGACCt           >  2:953310/1‑139 (MQ=255)
                                                                                                                    cgcACCAATCAGCGTAGCGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCATTATTATCTACCGACTGTc                       >  2:325346/1‑125 (MQ=255)
                                                                                                                    cgcACCAATCAGCGTAGCGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCATTATTATCTACCGACTGTc                       <  1:325346/125‑1 (MQ=255)
                                                                                                                           aTCAGCGTAGCGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCATTATTATCTACCGACTGTCGATGCTCGACCTGGTCGAAGa  <  2:1172837/139‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                
TCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCATTATTATCTACCGACTGTCGATGCTCGACCTGGTCGAAGA  >  NC_000913/3232074‑3232335

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑