Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 147,824 C→T A286V (GCA→GTA)  yadD → transposase_31 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913147,8240CT100.0% 343.7 / NA 97A286V (GCA→GTA) yadDtransposase_31 family protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (40/57);  total (40/57)

TGACGCTGGCGCAGTGGTTTGAAGAGAAAGGGATTGAGAAGGGGATTCAGCAGGGAAGACAGGAAGTAAGTCAGGAATTCGCCCAGCGTCTTCTGAGTAAAGGAATGTCTCGGGAAGACGTTGCAGAGATGGCAAATTTACCTCTTGCTGAGATTGATAAGGTAATTAACCTTATTTAAGTTACCTGTGTTATGACAGATGACGTGGGGTAAATTAATAACTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGCTCGAC  >  NC_000913/147692‑147958
                                                                                                                                    |                                                                                                                                      
tgaCGCTGGCGCAGTGGTTTGAAGAGAAAGGGATTGAGAAGGGGATTCAGCAGGGAAGACAGGAAGTAAGTCAGGAATTCGCCCAGCGTCTTCTGAGTAAAGGAATGTCTCGGGAAGACGTTGCAGAGATGGTAAAttt                                                                                                                                  <  2:378695/139‑1 (MQ=255)
       ggCGCAGTGGTTTGAAGAGAAAGGGATTGAGAAGGGGATTCAGCAGGGAAGACAGGAAGTAAGTCAGGAATTCGCCCAGCGTCTTCTGAGTAAAGGAATGTCTCGGGAAGACGTTGCAGAGATGGTAAATTTACCTCtt                                                                                                                           >  1:292077/1‑139 (MQ=255)
         cgcAGTGGTTTGAAGAGAAAGGGATTGAGAAGGGGATTCAGCAGGGAAGACAGGAAGTAAGTCAGGAATTCGCCCAGCGTCTTCTGAGTAAAGGAATGTCTCGGGAAGACGTTGCAGAGATGGTAAATTTACCTCTTGc                                                                                                                         <  1:1009133/139‑1 (MQ=255)
          gcAGTGGTTTGAAGAGAAAGGGATTGAGAAGGGGATTCAGCAGGGAAGACAGGAAGTAAGTCAGGAATTCGCCCAGCGTCTTCTGAGTAAAGGAATGTCTCGGGAAGACGTTGCAGAGATGGTAAATTTACCTCTTGCt                                                                                                                        <  2:71782/139‑1 (MQ=255)
            tgTGGTTTGAAGAGAAAGGGAATGAGAAGGGGATTCAGCAGGGAAGACAGGAAGTAAGTCAGGAATTCGCCCAGCGTCTTCTGAGTAAAGGAATGTCTCGGGAAGACGTTGCAGAGATGGTAAATCTGTCTCttataca                                                                                                                      >  1:740178/2‑134 (MQ=255)
                  ttGAAGAGAAAGGGATTGAGAAGGGGATTCAGCAGGGAAGACAGGAAGTAAGTCAGGAATTCGCCCAGCGTCTTCTGAGTAAAGGAATGTCTCGGGAAGACGTTGCAGAGATGGTAAATTTACCTCTTGCGGAGATTGa                                                                                                                <  2:415594/139‑1 (MQ=255)
                    gAAGAGAAAGGGATTGAGAAGGGGATTCAGCAGGGAAGACAGGAAGTAAGTCAGGAATTCGCCCAGCGTCTTCTGAGTAAAGGAATGTCTCGGGAAGACGTTGCAGAGATGGTAAATTTACCTCTTGCTGAGATTGATa                                                                                                              <  2:349427/139‑1 (MQ=255)
                    gAAGAGAAAGGGATTGAGAAGGGGATTCAGCAGGGAAGACAGGAAGTAAGTCAGGAATTCGCCCAGCGTCTTCTGAGTAAAGGAATGTCTCGGGAAGACGTTGCAGAGATGGTAAATTTACCTCTTGCTGAGATTGATa                                                                                                              <  1:128270/139‑1 (MQ=255)
                        agaAAGGGATTGAGAAGGGGATTCAGCAGGGAAGACAGGAAGTAAGTCAGGAATTCGCCCAGCGTCTTCTGAGTAAAGGAATGTCTCGGGAAGACGTTGCAGAGATGGTAAATTTACCTCTTGCTGAGATTGATAAGGt                                                                                                          >  1:610382/1‑139 (MQ=255)
                          aaaGGGATTGAGAAGGGGATTCAGCAGGGAAGACAGGAAGTAAGTCAGGAATTCGCCCAGCGTCTTCTGAGTAAAGGAATGTCTCGGGAAGACGTTGCAGAGATGGTAAATTTAc                                                                                                                                >  2:1441206/1‑115 (MQ=255)
                          aaaGGGATTGAGAAGGGGATTCAGCAGGGAAGACAGGAAGTAAGTCAGGAATTCGCCCAGCGTCTTCTGAGTAAAGGAATGTCTCGGGAAGACGTTGCAGAGATGGTAAATTTAc                                                                                                                                <  1:1441206/115‑1 (MQ=255)
                          aaaGGGATTGAGAAGGGGATTCAGCAGGGAAGACAGGAAGTAAGTCAGGAATTCGCCCAGCGTCTTCTGAGTAAAGGAATGTCTCGGGAAGACGTTGCAGAGATGGTAAATTTACCTCTTGCTGAGATTGATaa                                                                                                             >  1:563423/1‑134 (MQ=255)
                          aaaGGGATTGAGAAGGGGATTCAGCAGGGAAGACAGGAAGTAAGTCAGGAATTCGCCCAGCGTCTTCTGAGTAAAGGAATGTCTCGGGAAGACGTTGCAGAGATGGTAAATTTACCTCTTGCTGAGATTGATaa                                                                                                             <  2:563423/134‑1 (MQ=255)
                          aaaGGGATTGAGAAGGGGATTCAGCAGGGAAGACAGGAAGTAAGTCAGGAATTCGCCCAGCGTCTTCTGAGTAAAGGAATGTCTCGGGAAGACGTTGCAGAGATGGTAAATTTACCTCTTGCTGAGATTGATAAGGTaa                                                                                                        >  1:1250547/1‑139 (MQ=255)
                           aaGGGATTGAGAAGGGGATTCAGCAGGGAAGACAGGAAGTAAGTCAGGAATTCGCCCAGCGTCTTCTGAGTAAAGGAATGTCTCGGGAAGACGTTGCAGAGATGGTAAATTTACCTCTTGCTGAGATTGATAAGGTAAt                                                                                                       >  2:678622/1‑139 (MQ=255)
                           aaGGGATTGAGAAGGGGATTCAGCAGGGAAGACAGGAAGTAAGTCAGGAATTCGCCCAGCGTCTTCTGAGTAAAGGAATGGCTCGGGAAGACGTTGCAGAGATGGTAAATTTACCTCTTGCTGAGATTGATAAGGTAAt                                                                                                       <  2:1311867/139‑1 (MQ=255)
                            aGGGATTGAGAAGGGGATTCAGCAGGGAAGACAGGAAGTAAGTCAGGAATTCGCCCAGCGTCTTCTGAGTAAAGGAATGTCTCGGGAAGACGTTGCAGAGATGGTAAATTTACCTCTTGCTGAGATTGATAAGGTAAtt                                                                                                      >  1:20662/1‑139 (MQ=255)
                              ggATTGAGAAGGGGATTCAGCAGGGAAGACAGGAAGTAAGTCAGGAATTCGCCCAGCGTCTTCTGAGTAAAGGAATGTCTCGGGTAGACGTTGCAGAGATGGTAAATTTACCTCTTGCTGAGAGTGATAAGGTAATTaa                                                                                                    <  1:1031572/139‑1 (MQ=255)
                               gATTGAGAAGGGGATTCAGCAGGGAAGACAGGAAGTAAGTCAGGAATTCGCCCAGCGTCTTCTGAGTAAAGGAATGTCTCGGGAAGACGTTGCAGAGATGGTAAATTTACCTCTTGCTGAGATTGATAAGGTAATTAAc                                                                                                   <  1:678622/139‑1 (MQ=255)
                                   gagaAGGGGATTCAGCAGGGAAGACAGGAAGTAAGTCAGGAATTCGCCCAGCGTCTTCTGAGTAAAGGAATGTCTCGGGAAGACGTTGCAGAGATGGTAAATTTACCTCTTGCTGAGATTGATa                                                                                                              >  1:635173/1‑124 (MQ=255)
                                   gagaAGGGGATTCAGCAGGGAAGACAGGAAGTAAGTCAGGAATTCGCCCAGCGTCTTCTGAGTAAAGGAATGTCTCGGGAAGACGTTGCAGAGATGGTAAATTTACCTCTTGCTGAGATTGATa                                                                                                              <  2:635173/124‑1 (MQ=255)
                                   gagaAGGGGATTCAGCAGGGAAGACAGGAAGTAAGTCAGGAATTCGCCCAGCGTCTTCTGAGTAAAGGAATGTCTCGGGAAGACGTTGCAGAGATGGTAAATTTACCTCTTGCTGAGATTGATAAGGTAATTAACCTTa                                                                                               <  1:1197160/139‑1 (MQ=255)
                                     gaAGGGGATTCAGCAGGGAAGACAGGAAGTAAGTCAGGAATTCGCCCAGCGTCTTCTGAGTAAAGGAATGTCTCGGGAAGACGTTGCAGAGATGGTAAATTTACCTCTTGCTGAGATTGATAAGGTAATTAACCTTAtt                                                                                             <  1:1259454/139‑1 (MQ=255)
                                         gggATTCAGCAGGGAAGACAGGAAGTAAGTCGGGAATTCGCCCAGCGTCTTCTGAGTAAAGGAATGTCTCGGGAAGACGTTGCAGAGATGGTAAATTTACCTCTTGCTGAGATTGATAAGGTAATTaa                                                                                                    <  1:173493/128‑1 (MQ=255)
                                         gggATTCAGCAGGGAAGACAGGAAGTAAGTCGGGAATTCGCCCAGCGTCTTCTGAGTAAAGGAATGTCTCGGGAAGACGTTGCAGAGATGGTAAATTTACCTCTTGCTGAGATTGATAAGGTAATTaa                                                                                                    >  2:173493/1‑128 (MQ=255)
                                         gggATTCAGCAGGGAAGACAGGAAGTAAGTCAGGAATTCGCCCAGCGTCTTCTGAGTAAAGGAATGTCTCGGGAAGACGTTGCAGAGATGGTAAATTTACCTCTTGCTGAGATTGATAAGGTAATTAACCTTATTTAAg                                                                                         <  2:551372/139‑1 (MQ=255)
                                         gggATTCAGCAGGGAAGACAGGAAGTAAGTCAGGAATTCGCCCAGCGTCTTCTGAGTAAAGGAATGTCTCGGGAAGACGTTGCAGAGATGGTAAATTTACCACTTGCTGAGATTGATAAGGTAATTAACCTTATTTAAg                                                                                         >  2:935735/1‑139 (MQ=255)
                                              tCAGCAGGGAAGACAGGAAGTAAGTCAGGAATTCGCCCAGCGTCTTCTGAGTAAAGGAATGTCTCGGGAAGACGTTGCAGAGATGGTAAATTTACCTCTTGCTGAGATTGATAAGGTAATTAACCTTATTTAAGTTAcc                                                                                    <  2:1371189/139‑1 (MQ=255)
                                              tCAGCAGGGAAGACAGGAAGTAAGTCAGGAATTCGCCCAGCGTCTTCTGAGTAAAGGAATGTCTCGGGAAGACGTTGCAGAGATGGTAAATTTACCTCTTGCTGAGATTGATAAGGTAATTAACCTTATTTAAGTTAcc                                                                                    <  1:781582/139‑1 (MQ=255)
                                              tCAGCAGGGAAGACAGGAAGTAAGTCAGGAATTCGCCCAGCGTCTTCTGAGTAAAGGAATGTCTCGGGAAGACGTTGCAGAGATGGTAAATTTACCTCTTGCTGAGATTGATAAGGTAATTAACCTTATTTAAGTTAcc                                                                                    <  2:1406188/139‑1 (MQ=255)
                                              tCAGCAGGGAAGACAGGAAGTAAGTCAGGAATTCGCCCAGCGTCTTCTGAGTAAAGGAATGTCTCGGGAAGACGTTGCAGAGATGGTAAATTTACCTCTTGCTGAGATTGATAAGGTAATTAACCTTATTTAAGTTAcc                                                                                    <  2:1455604/139‑1 (MQ=255)
                                              tCAGCAGGGAAGACAGGAAGTAAGTCAGGAATTCGCCCAGCGTCTTCTGAGTAAAGGAATGTCTCGGGAAGACGTTGCAGAGATGGTAAATTTACCTCTTGCTGAGATTGATAAGGTAATTAACCTTATTTAAGTTAcc                                                                                    <  1:427547/139‑1 (MQ=255)
                                              tCAGCAGGGAAGACAGGAAGTAAGTCAGGAATTCGCCCAGCGTCTTCTGAGTAAAGGAATGTCTCGGGAAGACGTTGCAGAGATGGTAAATTTACCTCTTGCTGAGATTGATAAGGTAATTAACCTTATTTAAGTTAcc                                                                                    <  2:625289/139‑1 (MQ=255)
                                              tCAGCAGGGAAGACAGGAAGTAAGTCAGGAATTCGCCCAGCGTCTTCTGAGTAAAGGAATGTCTCGGGAAGACGTTGCAGAGATGGTAAATTTACCTCTTGCTGAGATTGATAAGGTAATTAACCTTATTTAAGTTAcc                                                                                    <  1:1212929/139‑1 (MQ=255)
                                                  cagGGAAGACAGGAAGTAAGTCAGGAATTCGCCCAGCGTCTTCTGAGTAAAGGAATGTCTCGGGAAGACGTTGCAGAGATGGTAAATTTACCTCTTGCTGAGATTGATAAGGTAATTAACCTTATTTAAGTTACCtgtg                                                                                <  2:969027/139‑1 (MQ=255)
                                                  cagGGAAGACAGGAAGTAAGTCAGGAATTCGCCCAGCGTCTTCTGAGTAAAGGAATGTCTCGGGAAGACGTTGCAGAGATGGTAAATTTACCTCTTGCTGAGATTGATAAGGTAATTAACCTTATTTAAGTTACCtgtg                                                                                <  2:578838/139‑1 (MQ=255)
                                                     ggAAGACAGGAAGTAAGTCAGGAATTCGCCCAGCGTCTTCTGAGTAAAGGAATGTCTCGGGAAGACGTTGCAGAGATGGTAAATTTACCTCTTGCTgaga                                                                                                                    >  1:947205/1‑100 (MQ=255)
                                                     ggAAGACAGGAAGTAAGTCAGGAATTCGCCCAGCGTCTACTGAGTAAAGGAATGTCTCGGGAAGACGTTGCAGAGATGGTAAATTTACCTCTTGCTgaga                                                                                                                    <  2:947205/100‑1 (MQ=255)
                                                       aaGACAGGAAGTAAGTCAGGAATTCGCCCAGCGTCTTCTGAGTAAAGGAATGTCTCGGGAAGACGTTGCAGAGATGGTAAATTTACCTCTTGCTGAGATTGATAAGGTAATTAACCTTATTTAAGTTACCTGTGTTATg                                                                           >  1:1015496/1‑139 (MQ=255)
                                                            aGGAAGTAAGTCAGGAATTCGCCCAGCGTCTTCTGAGTAAAGGAATGTCTCGGGAAGACGTTGCAGAGATGGTAAATTTACCTCTTGCTGAGATTGATAAGGTAATTAACCTTATTTAAGTTACCTGTGTTATGACAGa                                                                      <  1:878345/139‑1 (MQ=255)
                                                               aagtaagtCAGGAATTCGCCCAGCGTCTTCTGAGTAAAGGAATGTCTCGGGAAGACGTTGCAGAGATGGTAAATTTACCTCTTGCTGAGATTGATAAGGTAATTAACCTTATTTAAGTTACCTGTGTTATGACAGATGa                                                                   >  2:302451/1‑139 (MQ=255)
                                                                agtaagtCAGGAATTCGCCCAGCGTCTTCTGAGTAAAGGAATGTCTCGGGAAGACGTTGCAGAGATGGTAAATTTACCTCTTGCTgag                                                                                                                     >  2:631319/1‑88 (MQ=255)
                                                                agtaagtCAGGAATTCGCCCAGCGTCTTCTGAGTAAAGGAATGTCTCGGGAAGACGTTGCAGAGATGGTAAATTTACCTCTTGCTgag                                                                                                                     <  1:631319/88‑1 (MQ=255)
                                                                agtaagtCAGGAATTCGCCCAGCGTCTTCTGAGTAAAGGAATGTCTCGGGAAGACGTTGCAGAGATGGTAAATTTACCTCTTGCTGAGATTGATAAGGTAATTAACCTTATTTAAGTTACCTGTGTTATGACAGATGAc                                                                  <  1:246476/139‑1 (MQ=255)
                                                                agtaagtCAGGAATTCGCCCAGCGTCTTCTGAGGAAAGGAATGTCTCGGGAAGACGTTGCAGAGATGGTAAATTTACCTCTTGCTGAGATTGATAAGGTAATTAACCTTATTTAAGTTACCTGTGTTATGACAGATGAc                                                                  <  1:1103613/139‑1 (MQ=255)
                                                                 gtaagtCAGGAATTCGCCCAGCGTCTTCTGAGTAAAGGAATGTCTCGGGAAGACGTTGCAGAGATGGTAAATTTACCTCTTGCTGAGATTGATAAGGTAATTAACCTTATTTAAGTTACCTGTGTTATGACAGATGACg                                                                 <  1:782698/139‑1 (MQ=255)
                                                                    agtCAGGAATTCGCCCAGCGTCTTCTGAGTAAAGGAATGTCTCGGGAAGACGTTGCTGAGATGGTAAATTTACCTCTTGCTGAGATTGATaa                                                                                                             <  1:1214568/92‑1 (MQ=255)
                                                                    agtCAGGAATTCGCCCAGCGTCTTCTGAGTAAAGGAATGTCTCGGGAAGACGTTGCTGAGATGGTAAATTTACCTCTTGCTGAGATTGATaa                                                                                                             >  2:1214568/1‑92 (MQ=255)
                                                                    agtCAGGAATTCGCCCAGCGTCTTCTGAGTAAAGGAATGTCTCGGGAAGACGTTGCAGAGATGGTAAATTTACCTCTTGCTGAGATTGATAAGGTAATTAACCTTAtt                                                                                             >  2:514829/1‑108 (MQ=255)
                                                                    agtCAGGAATTCGCCCAGCGTCTTCTGAGTAAAGGAATGTCTCGGGAAGACGTTGCAGAGATGGTAAATTTACCTCTTGCTGAGATTGATAAGGTAATTAACCTTAtt                                                                                             <  1:514829/108‑1 (MQ=255)
                                                                     gtCAGGAATTCGCCCAGCGTCTTCTGAGTAAAGGAATGTCTCGGGAAGACGTTGCAGAGATGGTAAATTTACCTCTTGCTGAGATTGATAAGGTaa                                                                                                        <  2:385905/96‑1 (MQ=255)
                                                                     gtCAGGAATTCGCCCAGCGTCTTCTGAGTAAAGGAATGTCTCGGGAAGACGTTGCAGAGATGGTAAATTTACCTCTTGCTGAGATTGATAAGGTaa                                                                                                        >  1:385905/1‑96 (MQ=255)
                                                                       cAGGAATTCGCCCAGCGTCTTCTGAGTAAAGGAATGTCTCGGGAAGACGTTGCAGAGATGGTAAATTTACCTCTTGCTGAGATTGATAAGGTAATTAACCTTATTTAAGTTACCTGTGTTATGACAGATGACGTGGGGt                                                           <  2:292077/139‑1 (MQ=255)
                                                                       cAGGAATTCGCCCAGCGTCTTCTGAGTAAAGGAATGTCTCGGGAAGACGTTGCAGAGATGGTAAATTTACCTCTTGCTGAGATTGATAAGGTAATTAACCTTATTTAAGTTACCTGTGTTATGACAGATGACGTGGGGt                                                           <  2:1119902/139‑1 (MQ=255)
                                                                         ggAATTCGCCCAGCGTCTTCTGAGTAAAGGAATGTCTCGGGAAGACGTTGCAGAGATGGTAAATTTACCTCTTGCTGAGATTGATAAGGTAATTAACCTTATTTAAGTTACCTGTGTTATGACAGATGACGTGGGGTaa                                                         >  2:763889/1‑139 (MQ=255)
                                                                            aTTCGCCCAGCGTCTTCTGAGTAAAGGAATGTCTCGGGAAGACGTTGCAGAGATGGTAAATTTACCTCTTGCTga                                                                                                                      <  2:1148346/75‑1 (MQ=255)
                                                                            aTTCGCCCAGCGTCTTCTGAGTAAAGGAATGTCTCGGGAAGACGTTGCAGAGATGGTAAATTTACCTCTTGCTga                                                                                                                      >  1:1148346/1‑75 (MQ=255)
                                                                                gCCCAGCGTCTTCTGAGTAAAGGAATGTCTCGGGAAGACGTTGCAGAGATGGTAAATTTACCTCTTGCt                                                                                                                        <  1:426814/69‑1 (MQ=255)
                                                                                gCCCAGCGTCTTCTGAGTAAAGGAATGTCTCGGGAAGACGTTGCAGAGATGGTAAATTTACCTCTTGCt                                                                                                                        >  2:426814/1‑69 (MQ=255)
                                                                                gCCCAGCGTCTTCTGAGTAAAGGAATGTCTCGGGAAGACGTTGCAGAGATGGTAAATTTACCTCTTGCTGAGATTGATAAGGTAATTAACCTTATTTAAGTTACCTGTGTTATGACAGATGACGTGGGGTAAATtaata                                                  >  1:32854/1‑139 (MQ=255)
                                                                                gCCCAGCGTCTTCTGAGTAAAGGAATGTCTCGGGAAGACGTTGCAGAGATGGTAAATTTACCTCTTGCTGAGATTGATAAGGTAATTAACCTTATTTAAGTTACCTGTGTTATGACAGATGACGTGGGGTAAATtaata                                                  >  2:733734/1‑139 (MQ=255)
                                                                                           tctGAGTAAAGGAATGTCTCGGGAAGACGTTGCAGAGATGGTAAATTTACCTCTTGCt                                                                                                                        <  1:17468/58‑1 (MQ=255)
                                                                                           tctGAGTAAAGGAATGTCTCGGGAAGACGTTGCAGAGATGGTAAATTTACCTCTTGCt                                                                                                                        >  2:17468/1‑58 (MQ=255)
                                                                                                gTAAAGGAATGTCTCGGGAAGACGTTGCAGAGATGGTAAATTTACCTCTTGCTGAGATTGATAAGGTAATTAACCTTATTTAAGTTACCTGTGTTATGACAGATGACGTGGGGTAAATTAATAACTGGCGCCATCAGcc                                  >  1:543706/1‑139 (MQ=255)
                                                                                                    aGGAATGTCTCGGGAAGACGTTGCAGAGATGGTAAATTTACCTCTTGCTgag                                                                                                                     >  2:957129/1‑52 (MQ=255)
                                                                                                    aGGAATGTCTCGGGAAGACGTTGCAGAGATGGTAAATTTACCTCTTGCTgag                                                                                                                     <  1:957129/52‑1 (MQ=255)
                                                                                                    aGGAATGTCTCGGGAAGACGTTGCAGAGATGGTAAATTTACCTCTTGCTGAGATTGATAAGGTAATTAACCTTATTTAAGTTACCTGTGTTATGACAGATGACGTGGGGTAAATTAATAACTGGCGCCATCAGCCg                                 <  1:101925/136‑1 (MQ=255)
                                                                                                    aGGAATGTCTCGGGAAGACGTTGCAGAGATGGTAAATTTACCTCTTGCTGAGATTGATAAGGTAATTAACCTTATTTAAGTTACCTGTGTTATGACAGATGACGTGGGGTAAATTAATAACTGGCGCCATCAGCCg                                 >  2:101925/1‑136 (MQ=255)
                                                                                                     ggAATGTCTCGGGAAGACGTTGCAGAGATGGTAAATTTACCTCTTGCTGAGATTGATAAGGTAATTAACCTTATTTAAGTTACCTGTGTTATGACAGATGACGTGGGGTAAATTAATAACTGGCGCCATCAGCCGTAgc                             <  1:1275217/139‑1 (MQ=255)
                                                                                                      gAATGTCTCGGGAAGACGTTGCAGAGATGGTAAATTTACCTCTTGCTGAGATTGATAAGGTAATTAACCTTATTTAAGTTACCTGTGTTATGACAGATGACGTGGGGTAAATTAATAACTGGCGCCATCAGCCGTAgcg                            <  1:950162/139‑1 (MQ=255)
                                                                                                      gAATGTCTCGGGAAGACGTTGCAGAGATGGTAAATTTACCTCTTGCTGAGATTGATAAGGTAATTAACCTTATTTAAGTTACCTGTGTTATGACAGATGACGTGGGGTAAATTAATAACTGGCGCCATCAGCCGTAgcg                            <  2:610382/139‑1 (MQ=255)
                                                                                                       aaTGTCTCGGGAAGACGTTGCAGAGATGGTAAATTTACCTCTTGCTGAGATTGATAAGGTAATTAACCTTATTTAAGTTACCTGTGTTATGACAGATGACGTGGGGTAAATTAATAACTGGCGCCATCAGCCGTAgcgc                           <  2:32854/139‑1 (MQ=255)
                                                                                                       aaTGTCTCGGGAAGACGTTGCAGAGATGGTAAATTTACCTCTTGCTGAGATTGATAAGGTAATTAACCTTATTTAAGTTACCTGTGTTATGACAGATGACGTGGGGTAAATTAATAACTGGCGCCATCAGCCGTAgcgc                           <  2:437044/139‑1 (MQ=255)
                                                                                                       aaTGTCTCGGGAAGACGTTGCAGAGATGGTAAATTTACCTCTTGCTGAGATTGATAAGGTAATTAACCTTATTTAAGTTACCTGTGTTATGACAGATGACGTGGGGTAAATTAATAACTGGCGCCATCAGCCGTAgcgc                           <  2:20662/139‑1 (MQ=255)
                                                                                                        aTGTCTCGGGAAGACGTTGCAGAGATGGTAAATTTACCTCTTGCTGAGATTGATAAGGTAATTAACCTTATTTAAGTTACCTGTGTTATGACAGATGACGTGGGGTAAATTAATAACTGGCGCCATCAGCCGTAGCGcc                          <  1:935735/139‑1 (MQ=255)
                                                                                                         tGTCTCGGGAAGACGTTGCAGAGATGGTAAATTTACCTCTTGCTga                                                                                                                      >  2:297870/1‑46 (MQ=255)
                                                                                                         tGTCTCGGGAAGACGTTGCAGAGATGGTAAATTTACCTCTTGCTga                                                                                                                      <  1:297870/46‑1 (MQ=255)
                                                                                                         tGTCTCGGGAAGACGTTGCAGAGATGGTAAATTTACCTCTTGCTGAGATTGATAAGGTAATTAACCTTATTTAAGTTACCTgtgt                                                                               <  2:529477/85‑1 (MQ=255)
                                                                                                         tGTCTCGGGAAGACGTTGCAGAGATGGTAAATTTACCTCTTGCTGAGATTGATAAGGTAATTAACCTTATTTAAGTTACCTgtgt                                                                               >  1:529477/1‑85 (MQ=255)
                                                                                                           tctcGGGAAGACGTTGCAGAGATGGTAAATTTACCTCTTGCTGAGATTGATAAGGTAATTAACCTTa                                                                                               <  1:355417/67‑1 (MQ=255)
                                                                                                           tctcGGGAAGACGTTGCAGAGATGGTAAATTTACCTCTTGCTGAGATTGATAAGGTAATTAACCTTa                                                                                               >  2:355417/1‑67 (MQ=255)
                                                                                                              cGGGAAGACGTTGCAGAGATGGTAAATTTACCTCTTGCTGAGATTGATAAGGTAATTAACCTTATTTAAGTTACCTGTGTTATGACAGATGACg                                                                 >  2:1136691/1‑94 (MQ=255)
                                                                                                              cGGGAAGACGTTGCAGAGATGGTAAATTTACCTCTTGCTGAGATTGATAAGGTAATTAACCTTATTTAAGTTACCTGTGTTATGACAGATGACg                                                                 <  1:1136691/94‑1 (MQ=255)
                                                                                                              cGGGAAGACGTTGCAGAGATGGTAAATTTACCTCTTGCTGAGATTGATAAGGTAATTAACCTTATTTAAGTTACCTGTGTTATGACAGATGACGTGGGGTAAATTAATAACTGGCGCCATCAGCCGTAGCGCCAGTTaa                    >  2:442228/1‑139 (MQ=255)
                                                                                                                ggAAGACGTTGCAGAGATGGTAAATTTACCTCTTGCTGAGATTGATAAGGTAATTAACCTTATTTAAGTTACCTGTGTTATGACAGATGACGTGGGGTAAATTAATAACTGGCGCCATCAGCCGTAGCGCCAGTTAAGt                  >  1:568906/1‑139 (MQ=255)
                                                                                                                    gACGTTGCAGAGATGGTAAATTTACCTCTTGCTGAGATTGATAAGGTAATTAACCTTATTTAAGTTACCTGTGTTATGACAGa                                                                      >  2:364397/1‑83 (MQ=255)
                                                                                                                    gACGTTGCAGAGATGGTAAATTTACCTCTTGCTGAGATTGATAAGGTAATTAACCTTATTTAAGTTACCTGTGTTATGACAGa                                                                      <  1:364397/83‑1 (MQ=255)
                                                                                                                     aCGTTGCAGAGATGGTAAATTTACCTCTTGCTGAGATTGATAAGGTAATTAACCTTATTTAAGTTAc                                                                                     >  2:943685/1‑67 (MQ=255)
                                                                                                                     aCGTTGCAGAGATGGTAAATTTACCTCTTGCTGAGATTGATAAGGTAATTAACCTTATTTAAGTTAc                                                                                     <  1:943685/67‑1 (MQ=255)
                                                                                                                     aCGTTGCAGAGATGGTAAATTTACCTCTTGCTGAGATTGATAAGGTAATTAACCTTATTTAAGTTACCTGTGTTATGACAGATGACGTGGGGTAAATTAATAACTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTAc             >  1:774682/1‑139 (MQ=255)
                                                                                                                      cGTTGCAGAGATGGTAAATTTACCTCTTGCTGAGATTGATAAGGTAATTaa                                                                                                    >  2:937588/1‑51 (MQ=255)
                                                                                                                      cGTTGCAGAGATGGTAAATTTACCTCTTGCTGAGATTGATAAGGTAATTaa                                                                                                    <  1:937588/51‑1 (MQ=255)
                                                                                                                       gTTGCAGAGATGGTAAATTTACCTCTTGCTGAGATTGATAAGGTAATTAACCTTATTTAAGTTACCTGTGTTATGACAGATGACGTGGGGTAAATtaataa                                                 <  2:712086/101‑1 (MQ=255)
                                                                                                                       gTTGCAGAGATGGTAAATTTACCTCTTGCTGAGATTGATAAGGTAATTAACCTTATTTAAGTTACCTGTGTTATGACAGATGACGTGGGGTAAATtaataa                                                 >  1:712086/1‑101 (MQ=255)
                                                                                                                         tGCAGAGATGGTAAATTTACCTCTTGCTGAGATTGATAAGGTAATTAACCTTATTTAAGTTACCTGTGTTATGACAGATGACGTGGGGTAAATTAATAACTGGCGCCATCAGCCGTAgc                             <  2:750583/119‑1 (MQ=255)
                                                                                                                         tGCAGAGATGGTAAATTTACCTCTTGCTGAGATTGATAAGGTAATTAACCTTATTTAAGTTACCTGTGTTATGACAGATGACGTGGGGTAAATTAATAACTGGCGCCATCAGCCGTAgc                             >  1:750583/1‑119 (MQ=255)
                                                                                                                                aTGGTAAATTTACCTCTTGCTGAGATTGATAAGGTAATTAACCTTATTTAAGTTACCTGTGTTATGACAGATGACGTGGGGTAAATTAATAACTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGCTCGAc  >  2:476123/1‑139 (MQ=255)
                                                                                                                                    |                                                                                                                                      
TGACGCTGGCGCAGTGGTTTGAAGAGAAAGGGATTGAGAAGGGGATTCAGCAGGGAAGACAGGAAGTAAGTCAGGAATTCGCCCAGCGTCTTCTGAGTAAAGGAATGTCTCGGGAAGACGTTGCAGAGATGGCAAATTTACCTCTTGCTGAGATTGATAAGGTAATTAACCTTATTTAAGTTACCTGTGTTATGACAGATGACGTGGGGTAAATTAATAACTGGCGCCATCAGCCGTAGCGCCAGTTAAGTATTACGCCAGCTCGAC  >  NC_000913/147692‑147958

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑