Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,351,093	G→A	G190G (GGC→GGT)	dcuS ←	sensory histidine kinase in two‑component regulatory system with DcuR, regulator of anaerobic fumarate respiration

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,351,093	0	G	A	100.0%	215.1 / NA	62	G190G (GGC→GGT)	dcuS	sensory histidine kinase in two‑component regulatory system with DcuR, regulator of anaerobic fumarate respiration
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (30/32);  total (30/32)

CGACGCCTTCTTTGATAGACTGCAACATGGCCTGGCGTTGCTCAAACAGCGTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATGCCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTAGATGGGGGTAAATAC  >  NC_000913/4350957‑4351221                                                                                                                                         |                                                                                                                                 cgacgCCTTCTTTGATAGACTGCAACATGGCCTGGCGTTGCTCAAACAGCGTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATAcc                                                                                                                                >  1:282854/1‑139 (MQ=255)    cgCCTTCTTTGATAGACTGCAACATGGCCTGGCGTTGCTCAAACAGCGTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCaaa                                                                                                                             <  2:199151/139‑1 (MQ=255)        ttcttTGATAGACTGCAACATGGCCTGGCGTTGCTCAAACAGCGTGGAGATTTCGTAGGGTTTCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAaataat                                                                                                                         <  2:1047953/139‑1 (MQ=255)        ttcttTGATAGACTGCAACATGGCCTGGCGTTGCTCAAACAGCGTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAaataat                                                                                                                         <  1:698829/139‑1 (MQ=255)             tGATAGACTGCAACATGGCCTGGCGTTGCTCAAACAGCGTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGa                                                                                                                    <  2:728263/139‑1 (MQ=255)                  gACTGCAACATGGCCTGGCGTTGCTCAAACAGCGTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGa                                                                                                               <  1:96471/139‑1 (MQ=255)                  gACTGCAACATGGCCTGGCGTTGCTCAAACAGCGTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGa                                                                                                               <  1:761303/139‑1 (MQ=255)                   aCTGCAACATGGCCTGGCGTTGCTCAAACAGCGTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGAt                                                                                                              >  2:498520/1‑139 (MQ=255)                   aCTGCAACATGGCCTGGCGTTGCTCAAACAGCGTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGAt                                                                                                              >  2:420566/1‑139 (MQ=255)                    cTGCAACATGGCCTGGCGTTGCTCAAACAGCGTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATa                                                                                                             <  1:155924/139‑1 (MQ=255)                     tGCAACATGGCCTGGCGTTGCTCAAACAGCGTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATaa                                                                                                            >  1:21635/1‑139 (MQ=255)                         aCATGGCCTGGCGTTGCTCAAACAGCGTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCt                                                                                                        <  1:434541/139‑1 (MQ=255)                         aCATGGCCTGGCGTTGCTCAAACAGCGTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCt                                                                                                        >  2:434541/1‑139 (MQ=255)                                cTGGCGTTGCTCAAACAGCGTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGa                                                                                                 <  2:282854/139‑1 (MQ=255)                                 tGGCGTTGCTCAAACAGCGTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATCCCGACCAGATAATGCTCCAGCGAc                                                                                                <  2:994462/139‑1 (MQ=255)                                         cTCAAACAGCGTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTg                                                                                        >  1:1238531/1‑139 (MQ=255)                                                  cGTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATa                                                                                                             <  2:820211/109‑1 (MQ=255)                                                  cGTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATa                                                                                                             >  1:820211/1‑109 (MQ=255)                                                  cGTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCt                                                                                                        >  2:530859/1‑114 (MQ=255)                                                  cGTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCt                                                                                                        <  1:530859/114‑1 (MQ=255)                                                            tCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGc                                                                     >  2:1326783/1‑139 (MQ=255)                                                             cGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCt                                                                    <  2:1238531/139‑1 (MQ=255)                                                             cGTAAGGTTCCAGGCCGAAAAGGATTTTTTTCACTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAAATAATGCTCCAGCGACAGTCATTGATCTGTTGGGTCACACGGCt                                                                    <  2:561094/139‑1 (MQ=255)                                                                 gggTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATGATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAAc                                                                >  1:1119244/1‑139 (MQ=255)                                                                              aaaaGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCg                                                   >  1:26860/1‑139 (MQ=255)                                                                              aaaaGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCg                                                   >  1:416154/1‑139 (MQ=255)                                                                                 aGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGcca                                                <  2:26860/139‑1 (MQ=255)                                                                                  ggttttttttCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGccac                                               <  1:1370889/136‑1 (MQ=255)                                                                                           tCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAACACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAAt                                      <  1:1060117/139‑1 (MQ=255)                                                                                                 ccTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGttt                                <  1:1391422/139‑1 (MQ=255)                                                                                                    tAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTcaca                                                                         >  2:375632/1‑95 (MQ=255)                                                                                                    tAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTcaca                                                                         <  1:375632/95‑1 (MQ=255)                                                                                                    tAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATg                             >  1:1466206/1‑139 (MQ=255)                                                                                                       ccAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCGAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGAtt                          >  1:980552/1‑139 (MQ=255)                                                                                                         aGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGAtttt                        >  2:35601/1‑139 (MQ=255)                                                                                                         aGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGAtttt                        <  1:35601/139‑1 (MQ=255)                                                                                                           aaTGCAGGTGCCAATCAGTCCGACCAGCATACCAaataat                                                                                                                         >  1:823584/1‑40 (MQ=255)                                                                                                           aaTGCAGGTGCCAATCAGTCCGACCAGCATACCAaataat                                                                                                                         <  2:823584/40‑1 (MQ=255)                                                                                                           aaTGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCa                                                                                                     >  1:314383/1‑60 (MQ=255)                                                                                                           aaTGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCa                                                                                                     <  2:314383/60‑1 (MQ=255)                                                                                                           aaTGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGAt                                                                                      >  2:1460633/1‑75 (MQ=255)                                                                                                           aaTGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGAt                                                                                      <  1:1460633/75‑1 (MQ=255)                                                                                                           aaTGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCa                      >  1:720311/1‑139 (MQ=255)                                                                                                           aaTGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCa                      >  2:919769/1‑139 (MQ=255)                                                                                                               cAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGt                  >  2:834633/1‑139 (MQ=255)                                                                                                                 ggTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGcca                                                <  2:1107046/107‑1 (MQ=255)                                                                                                                 ggTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGcca                                                >  1:1107046/1‑107 (MQ=255)                                                                                                                 ggTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGttt                                <  2:1289580/123‑1 (MQ=255)                                                                                                                 ggTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGttt                                >  1:1289580/1‑123 (MQ=255)                                                                                                                  gTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTAGa               <  2:416154/139‑1 (MQ=255)                                                                                                                   tGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTAGAt              <  2:749099/139‑1 (MQ=255)                                                                                                                     ccAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATc                                                                                     >  1:676421/1‑66 (MQ=255)                                                                                                                     ccAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATc                                                                                     <  2:676421/66‑1 (MQ=255)                                                                                                                             tCCGACCAGCATACCAAATAATACCGACCAGATAATGc                                                                                                         <  2:109851/38‑1 (MQ=255)                                                                                                                             tCCGACCAGCATACCAAATAATACCGACCAGATAATGc                                                                                                         >  1:109851/1‑38 (MQ=255)                                                                                                                             tCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGa                                                                                                 <  2:1108713/46‑1 (MQ=255)                                                                                                                             tCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGa                                                                                                 >  1:1108713/1‑46 (MQ=255)                                                                                                                             tCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTc                       >  1:849829/1‑120 (MQ=255)                                                                                                                             tCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTc                       <  2:849829/120‑1 (MQ=255)                                                                                                                             tCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTAACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTAGATGGGTGTAAAt    >  2:341958/1‑139 (MQ=255)                                                                                                                              ccGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTAGATGGGGGTAAATa   <  1:341958/139‑1 (MQ=255)                                                                                                                               cGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTAGATGGGGGTAAATAc  >  1:100430/1‑139 (MQ=255)                                                                                                                                         |                                                                                                                                 CGACGCCTTCTTTGATAGACTGCAACATGGCCTGGCGTTGCTCAAACAGCGTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATGCCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTAGATGGGGGTAAATAC  >  NC_000913/4350957‑4351221

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑