| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 703,866 | A→G | intergenic (‑255/‑78) | nagB ← / → nagE | glucosamine‑6‑phosphate deaminase/N‑acetyl glucosamine specific PTS enzyme IIC, IIB, and IIA components |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 703,866 | 0 | A | G | 98.3% | 208.7 / NA | 59 | intergenic (‑255/‑78) | nagB/nagE | glucosamine‑6‑phosphate deaminase/N‑acetyl glucosamine specific PTS enzyme IIC, IIB, and IIA components |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (32/26); total (33/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
CTGGTTTAGCCAGTAAAAGGGAGTGATAATAACGATATTTGGTGACAAAACTCACAAAAGACACGCGTTTAATTTGCGATACGAATTAAATTTTCACACACTCTGTAGCAGATGATCTAACAATCTGATTACAGAACATCGGCAGTACAATTTGCAGCAAAATAAAAATACGGCTTGAA‑‑ACGAGCCAAATAGGGTTCTCGTAGGGGGAATAAGATGAATATTTTAGGTTTTTTCCAGCGACTCGGTAGGGCGTTACAGCTCCCTATCGCG > NC_000913/703731‑704000 | cTGGTTTAGCCAGTAAAAGGGAGTGATAATAACGATATTTGGTGACAAAACTCACAAAAGACACGCGTTTAATTTGCGATACGAATTAAATTTTCACACACTCTGTAGCAGATGATCTAACAATCTGATTACAGAGCAt < 1:393080/139‑1 (MQ=255)cTGGTTTAGCCAGTAAAAGGGAGTGATAATAACGATATTTGGTGACAAAACTCACAAAAGACACGCGTTTAATTTGCGATACGAATTAAATTTTCACACACTCTGTAGCAGATGATCTAACAATCTGATTACAGAGCAt < 2:360810/139‑1 (MQ=255) gTTTAGCCAGTAAAAGGGAGTGATAATAACGATATTTGGTGACAAAACTCACAAAAGACACGCGTTTAATTTGCGATACGAATTAAATTTTCACACACTCTGTAGCAGATGATCTAACAATCTGATTACAGAGCATCgg > 2:48056/1‑139 (MQ=255) tAGCCAGTAAAAGGGAGTGATAATAACGATATTTGGTGACAAAACTCACAAAAGACACGCGTTTAATTTGCGATACGAATTAAATTTTCACACACTCTGTAGCAGATGATCTAACAATCTGATTACAGAGCATCGGCAg > 2:1403526/1‑139 (MQ=255) tAAAAGGGAGTGATAATAACGATATTTGGTGACAAAACTCACAAAAGACACGCGTTTAATTTGCGATACGAATTAAATTTTCACACACTCTGTAGCAGATGATCTAACAATCTGATTACAGAGCATCGGCAGTACAAtt > 1:744327/1‑139 (MQ=255) tAAAAGGGAGTGATAATAACGATATTTGGTGACAAAACTCACAAAAGACACGCGTTTAATTTGCGATACGAATTAAATTTTCACACACTCTGTAGCAGATGATCTAACAATCTGATTACAGAGCATCGGCAGTACAAtt > 1:1267005/1‑139 (MQ=255) tAAAAGGGAGTGATAATAACGATATTTGGTGACAAAACTCACAAAAGACACGCGTTTAATTTGCGATACGAATTAAATTTTCACACACTCTGTAGCAGATGATCTAACAATCTGATTACAGAGCATCGGCAGTACAAtt > 2:972603/1‑139 (MQ=255) tAAAAGGGAGTGATAATAACGATATTTGGTGACAAAACTCACAAAAGACACGCGTTTAATTTGCGATACGAATTAAATTTTCACACACTCTGTAGCAGATGATCTAACAATCTGATTACAGAGCATCGGCAGTACAAtt > 2:1453254/1‑139 (MQ=255) aaaGGGAGTGATAATAACGATATTTGGTGACAAAACTCACAAAAGACACGCGTTTAATTTGCGATACGAATTAAATTTTCACACACTCTGTAGCAGATGATCTAACAATCTGATTACAGAGCATCGGCAGTACAATTTg < 1:1449316/139‑1 (MQ=255) gggAGTGATAATAACGATATTTGGTGACAAAACTCACAAAAGACACGCGTTTAATTTGCGATACGAATTAAATTTTCACACACTCTGTAGCAGATGATCTAACAATCTGATTACAGAGCAt > 1:1311331/1‑121 (MQ=255) gggAGTGATAATAACGATATTTGGTGACAAAACTCACAAAAGACACGCGTTTAATTTGCGATACGAATTAAATTTTCACACACTCTGTAGCAGATGATCTAACAATCTGATTACAGAGCAt < 2:1311331/121‑1 (MQ=255) gTGATAATAACGATATTTGGTGACAAAACTCACAAAAGACACGCGTTTAATTTGCGATACGAATTAAATTTTCACACACTCTGTAGCAGATGATCTAACAATCTGATTACAGAGCATCGGCAGTACAATTTGCAGCaaa > 1:285651/1‑139 (MQ=255) tGATAATAACGATATTTGGTGACAAAACTCACAAAAGACACGCGTTTAATTTGCGATACGAATTAAATTTTCACACACTCTGTAGCAGATGATCTAACAATCTGATTACAGAACATCGGCAGTACAATTTGCAGCaaaa > 1:929762/1‑139 (MQ=255) ataataACGATATTTGGTGACAAAACTCACAAAAGACACGCGTTTAATTTGCGATACGAATTAAATTTTCACACACTCTGTAGCAGATGATCTAACAATCTGATTACAGAGCATCGGCAGTACAATTTGCAGCaaaata > 2:784335/1‑139 (MQ=255) aCGATATTTGGTGACAAAACTCACAAAAGACACGCGTTTAATTTGCGATACGAATTAAATTTTCACACACTCTGTAGCAGATGATCTAACAATCTGATTACAGAGCATCGGCAGTACAATTTGCAGCaaaataaaaata < 2:744327/139‑1 (MQ=255) tatTTGGTGACAAAACTCACAAAAGACACGCGTTTAATTTGCGATACGAATTAAATTTTCACACACTCTGTAGCAGATGATCTAACAATCTGATTACAGAGCATCGGCAGCACAATTTGCAGCAAAATAAAAATACGGc > 1:1119588/1‑139 (MQ=255) tttGGTGACAAAACTCACAAAAGCCACGCGCTTAATTTGCGATACGAATTAAACTTTCACACACTCTGTAGCAGATGATCTAACAATCTGATTACAGAGCATCGGCAGTACAATTTGCAGCAAAATAAAAATACGGCtt > 2:378906/1‑139 (MQ=255) tttGGTGACAAAACTCACAAAAGACACGCGTTTAATTTGCGATACGAATTAAATTTTCACACACTCTGTAGCAGATGATCTAACAATCTGATTACAGAGCATCGGCAGTACAATTTGCAGCAAAATAAAAATACGGCtt < 1:972603/139‑1 (MQ=255) ggTGACAAAACTCACAAAAGACACGCGTTTAATTTGCGATACGAATTAAATTTTCTCACACTCTGTAGCAGATGATCTAACAATCTGATTACAGAGCATCGGCAGTACAATTTGCAGCAAAATAAAAATACGGCTTGaa < 1:542452/139‑1 (MQ=255) tGACAAAACTCACAAAAGACACGCGTTTAATTTGCGATACGAATTAAATTTTCACACACTCTGTAGCAGATGATCTAACAATCTGATTACAGAGCATCGGCAGTACAATTTGCAGCAAAATAAAAATACGGCTTGAA‑‑Ac > 2:583326/1‑139 (MQ=255) cAAAACTCACAAAAGACACGCGTTTAATTTGCGATACGAATTAAATTTTCACACACTCTGTAGCAGATGATCTAACAATCTGATTACAGAGCATCGGCAGTACAATTTGCAGCAAAATAAAAATACGGCTTGAA‑‑ACGAg < 1:648302/139‑1 (MQ=255) cAAAACTCACAAAAGACACGCGTTTAATTTGCGATACGAATTAAATTTTCACACACTCTGTAGCAGATGATCTAACAATCTGATTACAGAGCATCGGCAGTACAATTTGCAGCAAAATAAAAATACGGCTTGAA‑‑ACGAg < 1:1453254/139‑1 (MQ=255) aaaCTCACAAAAGACACGCGTTTAATTTGCGATACGAATTAAATTTTCACACACTCTGTAGCAGATGATCTAACAATCTGATTACAGAGCATCGGCAGTACAATTTGCAGCAAAATAAAAATACGGCTTGAA‑‑ACGAGcc > 1:170589/1‑139 (MQ=255) cTCACAAAAGACACGCGTTTAATTTGCGATACGAATTAAATTTTCACACACTCTGTAGCAGATGATCTAACAATCTGATTACAGAGCATCGGCAGTACAATTTGCAGCAAAATAAAAATACGGCTTGAA‑‑ACGAGCCaaa < 1:1403526/139‑1 (MQ=255) acacGCGTTTAATTTGCGATACGAATTAAATTTTCACACACTCTGTAGCAGATGATCTAACAATCTGATTACAGAGCATCGGCAGTACAATTTGCAGCAAAATAAAAATACGGCTTGAA‑‑ACGAGCCAAATAGGGTtctc > 1:569892/1‑139 (MQ=255) cacGGGTTTAATTTGCGATACGAATTAAATTTTCACACACTCTGTAGCAGATGATCTAACAATCTGATTACAGAGCATCGGCAGTACAATTTGCAGCAAAATAAAAATACGGCTTGAA‑‑ACTAGCCAAATAGGGTTCTCg < 1:967700/139‑1 (MQ=255) acGCGTTTAATTTGCGATACGAATTAAATTTTCACACACTCTGTAGCAGATGATCTAACAATCTGATTACAGAGCATCGGCAGTACAATTTGCAGCAAAATAAAAATACGGCTTGAA‑‑ACGAGCCAAATAGGGTTCTCGt < 2:170589/139‑1 (MQ=255) cgcgTTTAATTTGCGATACGAATTAAATTTTCACACACTCTGTAGCAGATGATCTAACAATCTGATTACAGAGCATCGGCAGTACAATTTGCAGCAAAATAAAAATACGGCTCGAA‑‑ACGAGCCAAATAGGGTTCTCGTa > 2:565323/1‑139 (MQ=255) gcgTTTAATTTGCGATACGAATTAAATTTTCACACACTCTGTAGCAGATGATCTAACAATCTGATTACAGAGCATCGGCAGTACAATTTGCAGCAAAATAAAAATACGGCTTGAA‑‑ACGAGCCAAATAGGGTTCTCGTAg < 1:583326/139‑1 (MQ=255) cgTTTAATTTGCGATACGAATTAAATTTTCACACACTCTGTAGCAGATGATCTAACAATCTGATTACAGAGCATCGGCAGTACAATTTGCAGCAAAATAAAAATACGGCTTGAA‑‑ACGAGCCAAATAGGGTTCTCGTAgg < 1:784335/139‑1 (MQ=255) cGATACGAATTAAATTTTCACACACTCTGTAGCAGATGATCTAACAATCTGATTACAGAGCATCGGCAGTACAATTTGCAGCAAAATAAAAATACGGCTTGAA‑‑ACGAGCCAAATAGGGTTCTCGTAGGGGGAATAAGAt > 1:551669/1‑139 (MQ=255) tGTAGCAGATGATCTAACAATCTGATTACAGAGCATCgg < 1:749425/39‑1 (MQ=255) tGTAGCAGATGATCTAACAATCTGATTACAGAGCATCgg > 2:749425/1‑39 (MQ=255) aGATGATCTAACAATCTGATTACAGAGCATCGGCAGTACAATTTGCAGCAAAATAAAAATACGGCTTGAA‑‑ACGAGCCAAATAGGGTTCTCGTAGGGGGAATAAGATGAATATTTTAGGTTTTTTCCAGCGACTCGGTa < 2:1230346/138‑1 (MQ=255) aGATGATCTAACAATCTGATTACAGAGCATCGGCAGTACAATTTGCAGCAAAATAAAAATACGGCTTGAA‑‑ACGAGCCAAATAGGGTTCTCGTAGGGGGAATAAGATGAATATTTTAGGTTTTTTCCAGCGACTCGGTa > 1:1230346/1‑138 (MQ=255) atgatCTAACAATCTGATTACAGAGCATCGGCAGTACAATTTgcag > 1:91297/1‑46 (MQ=255) atgatCTAACAATCTGATTACAGAGCATCGGCAGTACAATTTgcag < 2:91297/46‑1 (MQ=255) atgatCTAACAATCTGATTACAGAGCATCGGCAGTACAATTTGCAGCAAAATAAAAATACGGCTTGaa < 1:976798/68‑1 (MQ=255) atgatCTAACAATCTGATTACAGAGCATCGGCAGTACAATTTGCAGCAAAATAAAAATACGGCTTGaa > 2:976798/1‑68 (MQ=255) atgatCTAACAATCTGATTACAGAGCATCGGCAGTACAATTTGCAGCAAAATAAAAATACGGCTTGAA‑‑ACGAGCCAAATAGGGTTCTCGTa > 2:1243789/1‑91 (MQ=255) atgatCTAACAATCTGATTACAGAGCATCGGCAGTACAATTTGCAGCAAAATAAAAATACGGCTTGAA‑‑ACGAGCCAAATAGGGTTCTCGTa < 1:1243789/91‑1 (MQ=255) atgatCTAACAATCTGATTACAGAGCATCGGCAGTACAATTTGCAGCAAAATAAAAATACGGCTTGAA‑‑ACGAGCCAAATAGGGTTCTCGTAGGGGGAATAAGATGAATATTTTAg > 1:932097/1‑115 (MQ=255) atgatCTAACAATCTGATTACAGAGCATCGGCAGTACAATTTGCAGCAAAATAAAAATACGGCTTGAA‑‑ACGAGCCAAATAGGGTTCTCGTAGGGGGAATAAGATGAATATTTTAg < 2:932097/115‑1 (MQ=255) atgatCTAACAATCTGATTACAGAGCATCGGCAGTACAATTTGCAGCAAAATAAAAATACGGCTTGAA‑‑ACGAGCCAAATAGGGTTCTCGTAGGGGGAATAAGATGAATATTTTAGGTTTTTTCCAg > 1:319698/1‑126 (MQ=255) atgatCTAACAATCTGATTACAGAGCATCGGCAGTACAATTTGCAGCAAAATAAAAATACGGCTTGAA‑‑ACGAGCCAAATAGGGTTCTCGTAGGGGGAATAAGATGAATATTTTAGGTTTTTTCCAg < 2:319698/126‑1 (MQ=255) atgatCTAACAATCTGATTACAGAGCATCGGCAGTACAATTTGCAGCAAAATAAAAATACGGCTTGAA‑‑ACGAGCCAAATAGGGTTCTCGTAGGGGGAATAAGATGAATATTTTAGGTTTTTTCCAGCGACTCGGTAggg > 2:989341/1‑139 (MQ=255) atgatCTAACAATCTGATTACAGAGCATCGGCAGTACAATTTGCAGCAAAATAAAAATACGGCTTGAA‑‑ACGAGCCAAATAGGGTTCTCGTAGGGGGAATAAGATGAATATTTTAGGTTTTTTCCAGCGACTCGGTAggg > 2:200826/1‑139 (MQ=255) atgatCTAACAATCTGATTACAGAGCATCGGCAGTACAATTTGCAGCAAAATAAAAATACGGCTTGAA‑‑ACGAGCCAAATAGGGTTCTCGTAGGGGGAATAAGATGAATATTTTAGGTTTTTTCCAGCGACTCGGTAggg < 2:1267005/139‑1 (MQ=255) gatCTAACAATCTGATTACAGAGCATCGGCAGTACAATTTGCAGCAAAATAAAAATACGGCTTGAA‑‑Ac > 2:1070859/1‑68 (MQ=255) gatCTAACAATCTGATTACAGAGCATCGGCAGTACAATTTGCAGCAAAATAAAAATACGGCTTGAA‑‑Ac < 1:1070859/68‑1 (MQ=255) gatCTAACAATCTGATTACAGAGCATCGGCAGTACAATTTGCAGCAAAATAAAAATACGGCTTGAA‑‑ACGAGCCAAATAGGGTTCTCGTAGGGGGAATAAGATGAATATTTTAGGTTTTTTCCAGCGACTCGGTAGGGCg > 2:340218/1‑139 (MQ=255) gatCTAACAATCTGATTACAGAGCATCGGCAGTACAATTTGCAGCAAAATAAAAATACGGCTTGAA‑‑ACGAGCCAAATAGGGTTCTCGTAGGGGGAATAAGATGAATATTTTAGGTTTTTTCCAGCGACTCGGTAGGGCg > 1:706423/1‑139 (MQ=255) gatCTAACAATCTGATTACAGAGCATCGGCAGTACAATTTGCAGCAAAATAAAAATACGGCTTGAA‑‑ACGAGCCAAATAGGGTTCTCGTAGGGGGAATAAGATGAATATTTTAGGTTTTTTCCAGCGACTCGGTAGGGCg > 1:1466288/1‑139 (MQ=255) gatCTAACAATCTGATTACAGAGCATCGGCAGTACAATTTGCAGCAAAATAAAAATACGGCTTGAA‑‑ACGAGCCAAATAGGGTTCTCGTAGGGGGAATAAGATGAATATTTTAGGTTTTTTCCAGCGACTCGGTAGGGCg > 1:1070565/1‑139 (MQ=255) aaCAATCTGATTACAGAGCATCGGCAGTACAATTTGCAGCAAAATAAAAATACGGCTTGAAACACGAGCCAAATAGGGTTCTCGTAGGGGGAATAAGATGAATATTTTAGGTTTTTTCCAGCGACTCGGTAGGGCGTTa < 2:285651/139‑1 (MQ=255) aaCAATCTGATTACAGAGCATCGGCAGTACAATTTGCAGCAAAATAAAAATACGGCTTGAA‑‑ACGAGTCAAATAGAGTTCTCGTAGGGGGAATAAGATGAATATTTTAGGTTTTTTCCAGCGACTCGGTAGGGCGTTACa < 2:929762/139‑1 (MQ=255) tCTGATTACAGAGCATCGGCAGTACAATTTGCAGCAAAATAAAAATACGGCTTGAA‑‑ACGAGCCAAATAGGGTTCTCGTAGGGGGAATAAGATGAATATTTTAGGTTTTTTCCAGCGACTCGGTAGGGCGTTACAGCTcc > 2:1053534/1‑139 (MQ=255) tGATTACAGAGCATCGGCAGTACAATTTGCAGCAAAATAAAAATACGGCTTGAA‑‑ACGAGCCAAATAGGGTTCTCGTAGGGGGAATAAGATGAATATTTTAGGTTTTTTCCAGCGACTCGGTAGGGCGTTACAGCTCCCt > 1:1387705/1‑139 (MQ=255) cAGAGCATCGGCAGTACAATTTGCAGCAAAATAAAAATACGGCTTGAA‑‑ACGAGCCAAATAGGGTTCTCGTAGGGGGAATAAGATGAATATTTTAGGTTTTTTCCAGCGACTCGGTAGGGCGTTACAGCTCCCTATcgcg < 2:569892/139‑1 (MQ=255) | CTGGTTTAGCCAGTAAAAGGGAGTGATAATAACGATATTTGGTGACAAAACTCACAAAAGACACGCGTTTAATTTGCGATACGAATTAAATTTTCACACACTCTGTAGCAGATGATCTAACAATCTGATTACAGAACATCGGCAGTACAATTTGCAGCAAAATAAAAATACGGCTTGAA‑‑ACGAGCCAAATAGGGTTCTCGTAGGGGGAATAAGATGAATATTTTAGGTTTTTTCCAGCGACTCGGTAGGGCGTTACAGCTCCCTATCGCG > NC_000913/703731‑704000 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |