Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	709,223	C→T	T297I (ACC→ATC)	chiP →	chitoporin, uptake of chitosugars

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	709,223	0	C	T	100.0%	194.9 / NA	57	T297I (ACC→ATC)	chiP	chitoporin, uptake of chitosugars
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/29);  total (28/29)

CCAAAGCCAGCTACAAATTTGATATCGCCGGTAGCCCGTTAACCACCAGCTACCAGTTCTACGGTACCCGAGATAAAGTTGACGATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGACCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACGGTCAGCAGGGATACTTCCTGCAACGTATGACTCCAACCTACGCTTCCTCAAACGGTCGCCTGGAT  >  NC_000913/709088‑709353                                                                                                                                        |                                                                                                                                   ccAAAGCCAGCTACAAATTTGATATCGCCGGTAGCCCGTTAACCACCAGCTACCAGTTCTACGGTACCCGAGATAAAGTTGACGATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGATCtt                                                                                                                                 <  2:557023/139‑1 (MQ=255)         aGCTACAAATTTGATATCGCCGGTAGCCCGTTAACCACCAGCTACCAGTTCTACGGTACCCGAGATAAAGTTGACGATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGATCTTTGGTTAcc                                                                                                                         >  2:163382/1‑139 (MQ=255)           cTACAAATTTGATATCGCCGGTAGCCCGTTAACCACCAGCTACCAGTTCTACGGTACCCGAGATAAAGTTGACGATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGATCTTTGGTTACCgg                                                                                                                       >  2:692286/1‑139 (MQ=255)               aaaTTTGATATCGCCGGTAGCCCGTTAACCACCAGCTACCAGTTCTACGGTACCCGAGATAAAGTTGACGATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGATCTTTGGTTACCGggcgg                                                                                                                   <  1:1308514/139‑1 (MQ=255)                     gATATCGCCGGTAGCCCGTTAACCACCAGCTACCAGTTCTACGGTACCCGAGATAAAGTTGACGATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGATCTTTGGTTACCGggc                                                                                                                     >  1:1094994/1‑131 (MQ=255)                     gATATCGCCGGTAGCCCGTTAACCACCAGCTACCAGTTCTACGGTACCCGAGATAAAGTTGACGATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGATCTTTGGTTACCGggc                                                                                                                     <  2:1094994/131‑1 (MQ=255)                     gATATCGCCGGTAGCCCGTTAACCACCAGCTACCAGTTCTACGGTACCCGAGATAAAGTTGACGATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGATCTTTGGTTACCGGGCGGCTGACg                                                                                                             >  2:1463239/1‑139 (MQ=255)                     gATATCGCCGGTAGCCCGTTAACCACCAGCTACCAGTTCTACGGTACCCGAGATAAAGTTGACGATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGATCTTTGGTTACCGGGAGGCTGACg                                                                                                             >  1:873510/1‑139 (MQ=255)                           gCCGGTAGCCCGTTAACCACCAGCTACCAGTTCTACGGTACCCGAGATAAAGTTGACGATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGATCTTTGGTTACCGGGCGGCTGACGTAGTgg                                                                                                       >  1:239070/1‑139 (MQ=255)                                         aaCCACCAGCTACCAGTTCTACGGTACCCGAGATAAAGTTGACGATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGATCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGaa                                                                                         <  2:239070/139‑1 (MQ=255)                                             accaGCTACCAGTTCTACGGTACCCGAGATAAAGTTGACGATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGTAAGCGTTGATCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCa                                                                                     >  2:632180/1‑139 (MQ=255)                                               caGCTACCAGTTCTACGGTACCCGAGATAAAGTTGACGATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGATCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCAcc                                                                                   <  2:1007493/139‑1 (MQ=255)                                               caGCTACCAGTTCTACGGTACCCGAGATAAAGTTGACGATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGATCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCAcc                                                                                   <  1:972563/139‑1 (MQ=255)                                                             aCGGTACCCGAGATAAAGTTGACGATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGATCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGa                                                                     >  1:1278093/1‑139 (MQ=255)                                                             aCGGTACCCGAGATAAAGTTGACGATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGATCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGa                                                                     <  2:893102/139‑1 (MQ=255)                                                               ggTACCCGAGATAAAGTTGACGATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGATCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACg                                                                   <  2:1046795/139‑1 (MQ=255)                                                               ggTACCCGAGATAAAGTTGACGATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGATCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACg                                                                   >  1:63101/1‑139 (MQ=255)                                                               ggTACCCGAGATAAAGTTGACGATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGATCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACg                                                                   <  1:495472/139‑1 (MQ=255)                                                                gTACCCGAGATAAAGTTGACGATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGATCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACgg                                                                  <  1:720747/139‑1 (MQ=255)                                                                 tACCCGAGATAAAGTTGACGATCGCAGCGTCAACGACCTTTATGACGGCGCCGCCTGGCTGCAAGCGTTGATCTTTGGTTACCGCGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCACGTGGATTAAGGCTGACGGt                                                                 >  2:1117137/1‑139 (MQ=255)                                                                 tACCCGAGATAAAGTTGACGATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGATCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTg                                                                      >  2:1412768/1‑134 (MQ=255)                                                                 tACCCGAGATAAAGTTGACGATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGATCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTg                                                                      <  1:1412768/134‑1 (MQ=255)                                                                  aCCCGAGATAAAGTTGACGATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGATCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACGGTc                                                                <  2:1278093/139‑1 (MQ=255)                                                                   cccGAGATAAAGTTGACGATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGATCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACGGTca                                                               >  2:1228591/1‑139 (MQ=255)                                                                   cccGAGATAAAGTTGACGATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGATCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACGGTca                                                               >  2:729936/1‑139 (MQ=255)                                                                   cccGAGATAAAGTTGACGATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGATCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACGGTca                                                               >  1:1395435/1‑139 (MQ=255)                                                                   cccGAGATAAAGTTGACGATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGATCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACGGTca                                                               >  1:443955/1‑139 (MQ=255)                                                                   cccGAGATAAAGTTGACGATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGATCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACGGTca                                                               >  1:1138103/1‑139 (MQ=255)                                                                         aTAAAGTTGACGATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGATCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACGGTCAGCAggg                                                         <  1:163382/139‑1 (MQ=255)                                                                         aTAAAGTTGACGATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGATCTTTGGTTACCGAGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACGGTCAGCAggg                                                         <  2:756994/139‑1 (MQ=255)                                                                           aaaGTTGACGATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGATCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACGGTCAGCAGGGAt                                                       <  1:692286/139‑1 (MQ=255)                                                                                tGACGATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGATCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACGGTCAGCAGGGATACTTc                                                  <  1:1421813/139‑1 (MQ=255)                                                                                 gACGATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGATCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACGGTCAGCAGGGATACTTcc                                                 <  1:1228591/139‑1 (MQ=255)                                                                                  aCGATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGATCTTTGGTTACCGGGCGGCTGACg                                                                                                             <  2:850321/78‑1 (MQ=255)                                                                                  aCGATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGATCTTTGGTTACCGGGCGGCTGACg                                                                                                             >  1:850321/1‑78 (MQ=255)                                                                                  aCGATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGATCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACGGTCAGCAGGGATACTTCCt                                                >  1:1199834/1‑139 (MQ=255)                                                                                          aGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGATCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACTGTCAGCAGGGATACTTCCTGCAACGTa                                        <  2:873510/139‑1 (MQ=255)                                                                                                    aCCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGATCTTTGGTTACCGGGCGGCTGACGTCGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACGGTCAGCAGGGATACTTCCTGCAACGTATGACTCCAAc                              >  2:744004/1‑139 (MQ=255)                                                                                                    aCCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGATCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACGGTCAGCAGGGATACTTCCTGCAACGTATGACTCCAAc                              >  1:1059049/1‑139 (MQ=255)                                                                                                        ttATGACGGCACCGCCTGGCTGCAAGCGTTGATCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACGGTCAGCAGGGATACTTCCTGCAACGTATGACTCCAACCTAc                          >  1:1174174/1‑139 (MQ=255)                                                                                                         tATGACGGCACCGCCTGGCTGCAAGCGTTGATCTTTGGTTACCGggcgg                                                                                                                   <  2:1188739/49‑1 (MQ=255)                                                                                                         tATGACGGCACCGCCTGGCTGCAAGCGTTGATCTTTGGTTACCGggcgg                                                                                                                   >  1:1188739/1‑49 (MQ=255)                                                                                                          aTGACGGCACCGCCTGGCTGCAAGCGTTGATCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACGGTCAGCAGGGATACTTCCTGCAACGTATGACTCCAACCTACGc                        <  2:758852/139‑1 (MQ=255)                                                                                                            gACGGCACCGCCTGGCTGCAAGCGTTGATCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACGGTCAGCAGGGATACTTCCTGCAACGTATGACTCCAACCTACGCtt                      >  2:87500/1‑139 (MQ=255)                                                                                                               ggCACCGCCTGGCTGCAAGCGTTGATCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGa                                                                                          >  1:732287/1‑68 (MQ=255)                                                                                                               ggCACCGCCTGGCTGCAAGCGTTGATCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGa                                                                                          <  2:732287/68‑1 (MQ=255)                                                                                                                gCACCGCCTGGCTGCAAGCGTTGATCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCAc                                                                                    <  2:505523/73‑1 (MQ=255)                                                                                                                gCACCGCCTGGCTGCAAGCGTTGATCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCAc                                                                                    >  1:505523/1‑73 (MQ=255)                                                                                                                gCACCGCCTGGCTGCAAGCGTTGATCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCAc                                                                                    >  1:133896/1‑73 (MQ=255)                                                                                                                gCACCGCCTGGCTGCAAGCGTTGATCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCAc                                                                                    <  2:133896/73‑1 (MQ=255)                                                                                                                gCACCGCCTGGCTGCAAGCGTTGATCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACGGTCAGCAGGGATACTTCCTGCAACGTATGACTCCAACCTACGCTTCCTc                  >  2:120760/1‑139 (MQ=255)                                                                                                                 cACCGCCTGGCTGCAAGCGTTGATCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACGGTCAGCAggg                                                         >  2:900643/1‑99 (MQ=255)                                                                                                                 cACCGCCTGGCTGCAAGCGTTGATCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACGGTCAGCAggg                                                         <  1:900643/99‑1 (MQ=255)                                                                                                                      ccTGGCTGCAAGCGTTGATCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACGGTCAGCAGGGATACTTCCTGCAACGTATGACTCCAACCTACGCTTCCTCAAACgg            >  2:232679/1‑139 (MQ=255)                                                                                                                       cTGGCTGTAAGCGTTGATCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACGGTCAGCAGGGATACTTCCTGCAACGTATGACTCCAACCTACGCTTCCTCAAACGGt           <  1:632180/139‑1 (MQ=255)                                                                                                                         ggCTGCAAGCGTTGATCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACGGTCAGCAGGGATACTTCCTGCAACGTATGACTCCAACCTACGCTTCCTCAAACGGTCg         <  2:63101/139‑1 (MQ=255)                                                                                                                         ggCTGCAAGCGTTGATCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACGGTCAGCAGGGATACTTCCTGCAACGTATGACTCCAACCTACGCTTCCTCAAACGGTCg         <  1:87500/139‑1 (MQ=255)                                                                                                                                aGCGTTGATCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACGGTCAGCAGGGATACTTCCTGCAACGTATGACTCCAACCTACGCTTCCTCAAACGGTCGCCTGGat  <  2:1395435/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                                   CCAAAGCCAGCTACAAATTTGATATCGCCGGTAGCCCGTTAACCACCAGCTACCAGTTCTACGGTACCCGAGATAAAGTTGACGATCGCAGCGTCAACGACCTTTATGACGGCACCGCCTGGCTGCAAGCGTTGACCTTTGGTTACCGGGCGGCTGACGTAGTGGATTTGCGCCTCGAAGGCACCTGGGTTAAGGCTGACGGTCAGCAGGGATACTTCCTGCAACGTATGACTCCAACCTACGCTTCCTCAAACGGTCGCCTGGAT  >  NC_000913/709088‑709353

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑