| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,685,542 | (C)6→7 | coding (1047/1404 nt) | fumC ← | fumarate hydratase (fumarase C),aerobic Class II |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,685,536 | 1 | . | C | 96.3% | 91.3 / ‑3.2 | 27 | coding (1053/1404 nt) | fumC | fumarate hydratase (fumarase C),aerobic Class II |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base C (13/13); total (13/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCG‑CCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGAT > NC_000913/1685398‑1685664 | tCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAgcg < 2:1143374/139‑1 (MQ=255) cTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCG‑ccc > 1:690952/1‑139 (MQ=255) cACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCG‑ccccc > 2:1116858/1‑139 (MQ=255) gATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCG‑ccccccca < 2:359250/139‑3 (MQ=255) cGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCCCATGtt < 2:690952/139‑1 (MQ=255) gTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCCCATGTTGa < 1:248855/139‑1 (MQ=255) ccACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCCCATGTTGATCGCCAcgt < 1:1116858/139‑1 (MQ=255) gcgcAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCCCATGTTGATCGCCACGTCGTTc > 2:656157/1‑139 (MQ=255) gcgcAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCCCATGTTGATCGCCACGTCGTTc > 1:585568/1‑139 (MQ=255) gcgcAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCG‑cccc < 2:409825/115‑1 (MQ=255) gcgcAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCG‑cccc > 1:409825/1‑115 (MQ=255) gtgtTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAgcg‑c > 2:963042/1‑107 (MQ=255) gtgtTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAgcg‑c < 1:963042/107‑1 (MQ=255) gtgtTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCCCATGTTGATCGCCACGTCGTTCCCCAt < 1:656157/139‑1 (MQ=255) gtgtTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCCCATGTTGATCGCCACGTCGTTCCCCAt < 2:714984/139‑1 (MQ=255) aaaCTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCG‑CCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGAcagc < 1:895486/139‑1 (MQ=255) aaCTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACTGGAAGCGCCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGAcagc > 2:754364/1‑139 (MQ=255) aaCTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCCCATGTTGATCGCCACGTCGTTccc < 1:687468/125‑1 (MQ=255) aaCTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCCCATGTTGATCGCCACGTCGTTccc > 2:687468/1‑125 (MQ=255) aaCTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCG‑ccccc > 2:290975/1‑100 (MQ=255) aaCTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCG‑ccccc < 1:290975/100‑1 (MQ=255) ttCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCagag > 1:661382/1‑139 (MQ=255) cAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTcaca < 2:1022554/139‑1 (MQ=255) cAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTcaca < 2:805367/139‑1 (MQ=255) tgGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGc < 2:730803/97‑1 (MQ=255) tgGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGc > 1:730803/1‑97 (MQ=255) ttGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCagag > 1:1037066/1‑86 (MQ=255) ttGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCagag < 2:1037066/86‑1 (MQ=255) cGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGAcag > 1:296441/1‑76 (MQ=255) cGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGAcag < 2:296441/76‑1 (MQ=255) aaGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCCCATGTTGATCGCCACGTCGTTCCCCAt > 1:1117478/1‑63 (MQ=255) aaGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCCCATGTTGATCGCCACGTCGTTCCCCAt < 2:1117478/63‑1 (MQ=255) gttcagttcaAAGTTACCGGAAGCGCCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCAtt > 2:391837/1‑139 (MQ=255) ttcagttcaAAGTTACCGGAAGCGCCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCAttt > 1:23577/1‑139 (MQ=255) ttcaAAGTTACCGGAAGCGCCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCgg > 1:830214/1‑139 (MQ=255) caAAGTTACCGGAAGCGCCCCCCCATGTTGATCGCCACGTCGTTCCCCCTCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGa > 2:1056856/1‑139 (MQ=255) aCCGGAAGCGCCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGAt < 2:830214/139‑1 (MQ=255) | TCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCG‑CCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGAT > NC_000913/1685398‑1685664 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |