| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,001,304 | (G)7→8 | coding (960/2259 nt) | xdhA → | xanthine dehydrogenase, molybdenum binding subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,001,297 | 1 | . | G | 97.8% | 162.6 / ‑3.2 | 45 | coding (953/2259 nt) | xdhA | xanthine dehydrogenase, molybdenum binding subunit |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base G (20/24); total (20/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.65e-01 | |||||||||||
CGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGC‑‑GGGGGGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGATGCGTGGTTATGGCGCGCCACAAGTCGTATTTGCCGTT > NC_000913/3001160‑3001427 | cGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGC‑‑g > 2:749886/1‑139 (MQ=255)cGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGC‑‑g < 2:449778/139‑1 (MQ=255)cGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTACATCGCTTCTGC‑‑g > 1:198305/1‑139 (MQ=255) cAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGTAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGC‑‑ggg > 1:42289/1‑139 (MQ=255) cAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGC‑‑ggg > 1:817208/1‑139 (MQ=255) aCCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGC‑‑ggggg < 2:327679/139‑1 (MQ=255) cccGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGC‑‑ggggggggaata > 1:1192862/1‑134 (MQ=255) cccGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGC‑‑ggggggggaata < 2:1192862/139‑6 (MQ=255) cccGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGC‑‑ggggggggaata < 2:66174/139‑6 (MQ=255) cACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTTAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCG‑GGGGGGGAATAAAGTc > 2:844293/1‑139 (MQ=255) gCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATGGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCG‑GGGGGGGAATAAAGTCGCt < 2:882264/139‑1 (MQ=255) cTTTTGCCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCTCTTCTGCGGGGGGGGGAATAAAGTCGCt < 1:749886/139‑1 (MQ=255) tGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCG‑GGGGGGGAATAAAGTCGCTTACCTTTATcc > 1:1055683/1‑139 (MQ=255) gACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCG‑GGGGGGGAATAAAGTCGCTTACCTTTATCCt > 1:44949/1‑139 (MQ=255) gACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCG‑GGGGGGGAATAAAGTCGCTTACCTTTATCCt > 1:577865/1‑139 (MQ=255) cGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCG‑GGGGGGGAATAAAGTCGCTTACCTTTATCCTCg < 2:577865/139‑1 (MQ=255) cAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGC‑‑GGGGGGGAATAAAGTCGCTTACCTTTATCCTCGTtgtg < 2:220077/139‑1 (MQ=255) aTGGGCGTGAACCGCGACGGAACAGTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCG‑GGGGGGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGcc < 1:676152/139‑1 (MQ=255) tGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATTTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCG‑GGGGGGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCtt < 1:622933/139‑1 (MQ=255) gAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCG‑GGGGGGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGa < 2:979946/139‑1 (MQ=255) cATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCG‑GGGGGGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCt < 1:337644/139‑1 (MQ=255) tGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCG‑GGGGGGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCt < 1:497449/139‑1 (MQ=255) ttATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCG‑GGGGGGGAATAAAGTCGCTTa < 2:300741/88‑1 (MQ=255) ttATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCG‑GGGGGGGAATAAAGTCGCTTa > 1:300741/1‑88 (MQ=255) ggATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCG‑GGGGGGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTc > 1:419350/1‑139 (MQ=255) gATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCG‑GGGGGGGAATAAAGTCGCt > 2:1122502/1‑76 (MQ=255) gATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCG‑GGGGGGGAATAAAGTCGCt < 1:1122502/76‑1 (MQ=255) gATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCG‑GGGGGGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCaaa > 1:173364/1‑114 (MQ=255) gATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCG‑GGGGGGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCaaa < 2:173364/114‑1 (MQ=255) ttCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCG‑GGGGGGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTg < 1:506306/139‑1 (MQ=255) cTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCG‑GGGGGGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGt > 1:299486/1‑139 (MQ=255) cTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGC‑‑gg < 1:791191/52‑1 (MQ=255) cTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGC‑‑gg > 2:791191/1‑52 (MQ=255) tAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGCG‑GGGGGGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGAt > 1:671589/1‑139 (MQ=255) aaCACCGGCGCTTACGCATCTCACGGGCACTCCATCGCTTCTGCG‑GGGGGGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGATg < 1:844293/139‑1 (MQ=255) gcgcTTATGCATCTCACGGGCACTCCATCGCTTCTGCGGGGGGGGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGCCTGGTGCGATGCGTGGt > 1:77925/1‑139 (MQ=255) gcgcTTATGCATCTCACGGGCACTCCATCGCTTCTGCG‑GGGGGGGAATAAAGTCGCTTAc > 2:399078/1‑60 (MQ=255) gcgcTTATGCATCTCACGGGCACTCCATCGCTTCTGCG‑GGGGGGGAATAAAGTCGCTTAc < 1:399078/60‑1 (MQ=255) gcgcTTATGCATCTCACGGGCACTCCATCGCTTCTGCG‑GGGGGGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGa > 1:723582/1‑97 (MQ=255) gcgcTTATGCATCTCACGGGCACTCCATCGCTTCTGCG‑GGGGGGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGa < 2:723582/97‑1 (MQ=255) ctcACGGGCACTCCATCGCTTCTGCG‑GGGGGGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTa > 1:744400/1‑74 (MQ=255) ctcACGGGCACTCCATCGCTTCTGCG‑GGGGGGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTa < 2:744400/74‑1 (MQ=255) cACGGGCACTCCATCGCTTCTGCG‑GGGGGGGAATAAAg < 1:781644/38‑1 (MQ=255) cACGGGCACTCCATCGCTTCTGCG‑GGGGGGGAATAAAg > 2:781644/1‑38 (MQ=255) cTCCATCGCTTCTGCG‑GGGGGGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCa < 2:613991/71‑1 (MQ=255) cTCCATCGCTTCTGCG‑GGGGGGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCa > 1:613991/1‑71 (MQ=255) cTCCATCGCTTCTGCG‑GGGGGGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTg < 1:90312/79‑1 (MQ=255) cTCCATCGCTTCTGCG‑GGGGGGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTg > 2:90312/1‑79 (MQ=255) cTCCATCGCTTCTGCG‑GGGGGGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGATg > 1:450072/1‑110 (MQ=255) cTCCATCGCTTCTGCG‑GGGGGGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGATg < 2:450072/110‑1 (MQ=255) cATCGCTTCTGCG‑GGGGGGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGATGCGTGGTTATGGCGCGCCACAAGTCGTATTTGc > 1:200245/1‑139 (MQ=255) tCGCTTCTGCG‑GGGGGGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGA‑CTGCTATACCAACCTCCCCTCGGCTGGTGCGATGCGTGGTTATGGCGCGCca < 1:369508/122‑1 (MQ=255) tCGCTTCTGCG‑GGGGGGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGA‑CTGCTATACCAACCTCCCCTCGGCTGGTGCGATGCGTGGTTATGGCGCGCca > 2:369508/1‑122 (MQ=255) cGCTTCTGCG‑GGGGGGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGATGCGTGGTTATGGCGCGCCACAAGTCGTATTTGCCGt < 2:419350/139‑1 (MQ=255) gCTTCTGCG‑GGGGGGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTTCGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGATGCGTGGTTATGGCGCGCCACAAGTCGTATTTGCCGtt < 1:659573/139‑1 (MQ=255) gCTTCTGCG‑GGGGGGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGATGCGTGGTTATGGCGCGCCACAAGTCGTATTTGCCGtt < 2:1173741/139‑1 (MQ=255) | CGCAACCCGTACCCGCCACGCTTTTACCATTGACGGGCAAATGGGCGTGAACCGCGACGGAACATTGAAAGGTTATAGTCTGGATGTTCTGTCTAACACCGGCGCTTATGCATCTCACGGGCACTCCATCGCTTCTGC‑‑GGGGGGGAATAAAGTCGCTTACCTTTATCCTCGTTGTGCCTACGCTTACAGTTCAAAGACCTGCTATACCAACCTCCCCTCGGCTGGTGCGATGCGTGGTTATGGCGCGCCACAAGTCGTATTTGCCGTT > NC_000913/3001160‑3001427 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |