| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,561,426 | (G)5→6 | coding (27/831 nt) | glpG ← | rhomboid intramembrane serine protease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,561,421 | 1 | . | G | 97.6% | 149.5 / ‑3.2 | 41 | coding (32/831 nt) | glpG | rhomboid intramembrane serine protease |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base G (21/19); total (22/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.25e-01 | |||||||||||
AAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCATGTAATCAACAAACGCCTGCGCCACGC‑GGGGGTTAGCAAAAGAGGTAATCATCAACATCGTTGCTGTCGCTTATTCCACACAAAAGGGGACAGTATAAAGCGTTACGCGCCGTACGCCACCTCTGCGGGAAACTGACGTTGCCAGGCTTCAAAGCCGC > NC_000913/3561288‑3561552 | aaaaCGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCATGTAATCAACAAACGCCTGCGCCACGC‑ggggg < 1:227613/139‑1 (MQ=255) ccAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCATGTAATCAACAAACGCCTGCGCCACGCGGGGGGTTAGCaa < 2:629735/139‑1 (MQ=255) gCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCATGTAATCAACAAACGCCTGCGCCACGCGGGGGGTTAGCAAAag < 2:291598/139‑1 (MQ=255) gCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCATGTAATCAACAAACGCCTGCGCCACGCGGGGGGTTAGCAAAAGAGGTa < 2:484434/139‑1 (MQ=255) gCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCATGTAATCAACAAACGCCTGCGCCACGCGGGGGGTTAGCAAAAGAGGTa < 2:930095/139‑1 (MQ=255) aCGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCATGTAATCAACAAACGCCTGCGCCACGCGGGGGGTTAGCAAAAGAGGTAatcatc > 1:627779/1‑139 (MQ=255) aCGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCATGTAATCAACAAACGCCTGCGCCACGC‑ggggggtt > 1:865547/1‑117 (MQ=255) aCGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTCGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCATGTAATCAACAAACGCCTGCGCCACGC‑ggggggtt < 2:865547/120‑4 (MQ=255) cgcgCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCATGTAATCAACAAACGCCTGCGCCACGCGGGGGGTTAGCAAAAGAGGTAAtcatca > 2:164462/1‑139 (MQ=255) tGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCATGTAATCAACAAACGCCTGCGCCACGCGGGGGGTTAGCAAAAGAGGTAATCATCAACATCGTTGCt < 1:142902/139‑1 (MQ=255) tGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCATGTAATCAACAAACGCCTGCGCCACGCGGGGGGTTAGCAAAAGAGGTAATCATCAACATCGTTGCt < 1:164462/139‑1 (MQ=255) tGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCATGTAATCAACAAACGCCTGCGCCACGCGGGGGGTTAGCAAAAGAGGTAATCATCAACATCGTTGCt < 2:339501/139‑1 (MQ=255) cTCATCATCCAGCCAAACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCATGTAATCAACAAACGCCTGCGCCACGCGGGGGGTTAGTAAAAGAGGTAATCATCAAAATCGTTGCTGTCGc < 2:582925/139‑1 (MQ=255) aTCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCATGTAATCAACAAACGCCTGCGCCACGCGGGGGGTTAGCAAAAGAGGTa > 2:714/1‑113 (MQ=255) aTCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCATGTAATCAACAAACGCCTGCGCCACGCGGGGGGTTAGCAAAAGAGGTa < 1:714/113‑1 (MQ=255) aTCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCATGTAATCAACAAACGCCTGCGCCACGCGGGGGGTTAGCAAAAGAGGTAATCATCAACATCGTTGCTGTCGCTTa > 2:504993/1‑139 (MQ=255) tCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCATGTAATCAACAAACGCCTGCGCCACGCGGGGGGTTAGCAAAAGAGGTAATCATCAACATCGTTGCTGTCGCTTATTCCACACAAAAgggg < 1:504993/139‑1 (MQ=255) cGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCATGTAATCAACAAACGCCTGCGCCACGCGGGGGGTTAGCAAAAGAGGTAATCATCAACATCGTTGCTGTCGCTTATTCCACACAAAAGGGGa < 1:6149/139‑1 (MQ=255) cGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCATGTAATCAACAAACGCCTGCGCCACGCGGGGGGTTAGCAAAAGAGGTAATCATCAACATCGTTGCTGTCGCTTATTCCACACAAAAGGGGa < 2:627779/139‑1 (MQ=255) cTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCATGTAATCAACAAACGCCTGCGCCACGCGGGGGGTTAGCAAAAGAGGTAATCATCAACATCGTTGCTGTCGCTTATTCCACACAAAAGGGGACa > 2:107792/1‑139 (MQ=255) gTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCATGTAATCAACAAACGCCTGCGCCACGCGGGGGGTTAGCAAAAGAGGTAATCATCAACATCGTTGCTGTCGCTTATTCCACACAAAAGGGGAc > 1:625879/1‑133 (MQ=255) gTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCATGTAATCAACAAACGCCTGCGCCACGCGGGGGGTTAGCAAAAGAGGTAATCATCAACATCGTTGCTGTCGCTTATTCCACACAAAAGGGGAc < 2:625879/133‑1 (MQ=255) aTGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCATGTAATCAACAAACGCCTGCGCCACGCGGGGGGTTAGCAAAAGAGGTAATCATCAACATCGTTGCTGTCGCTTATTCCACACAAAAGGGGACAGTATAAAg > 1:656020/1‑139 (MQ=255) tgAATCGTGAGGATAACACCCTGCGTCGCCATGTAATCAACAAACGCCTGCGCCACGCGGGGGGTTAGCAAAAGAGGTAATCATCAACATCGTTGCTGTCGCTTATTCCACACAAAAGGGGACAGTATAAAGCGTTAcg > 2:770216/1‑139 (MQ=255) tgAATCGTGAGGATAACACCCTGCGTCGCCATGTAATCAACAAACGCCTGCGCCACGCGGGGGGTTAGCAAAAGAGGTAATCATCAACATCGTTGCTGTCGCTTATTCCACACAAAAGGGGACAGTATAAAGCGTTAcg > 2:1188054/1‑139 (MQ=255) gAATCGTGAGGATAACACCCTGCGTCGCCATGTAATCAACAAACGCCTGCGCCACGCGGGGGGTTAGCAAAAGAGGTa > 1:970834/1‑78 (MQ=255) gAATCGTGAGGATAACACCCTGCGTCGCCATGTAATCAACAAACGCCTGCGCCACGCGGGGGGTTAGCAAAAGAGGTa < 2:970834/78‑1 (MQ=255) gAATCGTGAGGATAACACCCTGCGTCGCCATGTAATCAACAAACGCCTGCGCCACGCGGGGGGTTAGCAAAAGAGGTAATCATCAACATCGTTGCTGTCGCTTATTCCACACAAAAGGGGACAGTATAAAGCGTTAcgc > 2:1028511/1‑139 (MQ=255) tCGTGAGGATAACACCCTGCGTCGCCATGTAATCAACAAACGCCTGCGCCACGCGGGGGGTTAGCAAAAGAGGTAATCATCAACATCGTTGCTGTCGCTTATTCCACACAAAAGGGGACAGTATAAAGCGTTACGCGcc > 1:502862/1‑139 (MQ=255) tGAGGATAACACCCTGCGTCGCCATGTAATCAACAAACGCCTGCGCCACGCGGGGGGTTAGCAAAAGAGGTAATCATCAACATCGTTGCTGTCGCTTATTCCACACAAAAGGGGa > 1:927136/1‑115 (MQ=255) tGAGGATAACACCCTGCGTCGCCATGTAATCAACAAACGCCTGCGCCACGCGGGGGGTTAGCAAAAGAGGTAATCATCAACATCGTTGCTGTCGCTTATTCCACACAAAAGGGGa < 2:927136/115‑1 (MQ=255) aTAACACCCTGCGTCGCCATGTAATCAACAAACGCCTGCGCCACGCGGGGGGTTAGCAAAAGAGGTAATCATCAACATCGTTGCTGTCGCTTATTCCACACAAAAGGGGACAGTATAAAGCGTTACGCGCCGTACGCCa > 1:1111269/1‑139 (MQ=255) aaCACCCTGCGTCGCCATGTAATCAACAAACGCCTGCGCCACGCGGGGGGTTAGCAAAAGAGGTAATCATCAACATCGTTGCTGTCGCTTATTCCACACAAAAGGGGACAGTATAAAGCGTTACGCGCCGTACGCCAcc > 2:1161571/1‑139 (MQ=255) cTGCGTCGCCATGTAATCAACAAACGCCTGCGCCACGCGGGGGGTTAGCAAAAGAGGTAATCATCAACATCGTTGCTGTCGCTTATTCCACACAAAAGGGGACAGTATAAAGCGTTACGCGCCGTACGCCACCTCTGCg < 1:1028511/139‑1 (MQ=255) tGCGTCGCCATGTAATCAACAAACGCCTGCGCCACGCGGGGGGTTAGCAAAAGAGGTAATCATCAACATc > 2:262432/1‑70 (MQ=255) tGCGTCGCCATGTAATCAACAAACGCCTGCGCCACGCGGGGGGTTAGCAAAAGAGGTAATCATCAACATc < 1:262432/70‑1 (MQ=255) tGCGTCGCCATGTAATCAACAAACGCCTGCGCCACGCGGGGGGTTAGCAAAAGAGGTAATCATCAACATCGTTGCTGTCGCTTATTCCACACAAAAGGGGACAGTATAAAGCGTTACGCGCCGTACGCCACCTCTGCgg > 2:1062435/1‑139 (MQ=255) tGCGTCGCCATGTAATCAACAAACGCCTGCGCCACGCGGGGGGTTAGCAAAAGAGGTAATCATCAACATCGTTGCTGTCGCTTATTCCACACAAAAGGGGACAGTATAAAGCGTTACGCGCCGTACGCCACCTCTGCgg > 1:865355/1‑139 (MQ=255) cATGTAATCAACAAACGCCTGCGCCACGCGGGGGGTTAGCAAAAGAGGTAATCATCAACATCGTTGCTGTCGCTTATTCCACACAAAAGGGGACAGTATAAAGCGTTACGCGCCGTACGCCACCTCTGCGGGAAACTGa < 1:1188054/139‑1 (MQ=255) acaaACGCCTGCGCCACGCGGGGGGTTAGCAAAAGAGGTAATCATCAACATCGTTGCTGTCGCTTATTCCACACAAAAGGGGACAGTATAAAGCGTTACGCGCCGTACGCCACCTCTGCGGGAAACTGACGTTGCCAgg > 2:185391/1‑139 (MQ=255) aaaCGCCTGCGCCACGCGGGGGGTTAGCAAAAGAGGTAATCATCAACATCGTTGCTGTCGCTTATTCCACACAAAAGGGGACAGTATAAAGCGTTACgcgc > 2:1125692/1‑101 (MQ=255) aaaCGCCTGCGCCACGCGGGGGGTTAGCAAAAGAGGTAATCATCAACATCGTTGCTGTCGCTTATTCCACACAAAAGGGGACAGTATAAAGCGTTACgcgc < 1:1125692/101‑1 (MQ=255) aCGCCTGCGCCACGCGGGGGGTTAGCAAAAGAGGTAATCATCAACATCGTTGCTGTCGCTTATTCCACACAAAAGGGGACAGTATAAAGCGTTACGCGCCGTACGCCACCTCTGCGGGAAACTGACGTTGCCAGGCTTc > 2:194095/1‑139 (MQ=255) cgcCACGC‑GGGGGTTAGCAAAAGAGGTAATCATCAACATCGTTGCTGTCGCTTATTCCACACAAAAGGGGACAGTATAAAGCGTTACGCGCCGTACGCCACCTCTGCGGGAAACTGACGTTGCCAGGCTTCAAAgccgc > 2:467745/1‑139 (MQ=255) | AAAACGCGCCAGCTCCGCCCGTACGCGCTCGGCCTGGGACTCATCCGCCAGCCAGACATCGCTTTGGTTATGTTGTTGAATCGTGAGGATAACACCCTGCGTCGCCATGTAATCAACAAACGCCTGCGCCACGC‑GGGGGTTAGCAAAAGAGGTAATCATCAACATCGTTGCTGTCGCTTATTCCACACAAAAGGGGACAGTATAAAGCGTTACGCGCCGTACGCCACCTCTGCGGGAAACTGACGTTGCCAGGCTTCAAAGCCGC > NC_000913/3561288‑3561552 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |