Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	95,633	G→A	L328L (CTG→CTA)	murF →	UDP‑N‑acetylmuramoyl‑tripeptide:D‑alanyl‑D‑ alanine ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	95,633	0	G	A	100.0%	161.2 / NA	47	L328L (CTG→CTA)	murF	UDP‑N‑acetylmuramoyl‑tripeptide:D‑alanyl‑D‑ alanine ligase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/24);  total (23/24)

GCGAATGCGCTGGCAGCCGCTGCGCTCTCCATGTCCGTGGGCGCAACGCTTGATGCTATCAAAGCGGGGCTGGCAAATCTGAAAGCTGTTCCAGGCCGTCTGTTCCCCATCCAACTGGCAGAAAACCAGTTGCTGCTCGACGACTCCTACAACGCCAATGTCGGTTCAATGACTGCAGCAGTCCAGGTACTGGCTGAAATGCCGGGCTACCGCGTGCTGGTGGTGGGCGATATGGCGGAACTGGGCGCTGAAAGCGAAGCCTGCCATG  >  NC_000913/95499‑95766                                                                                                                                       |                                                                                                                                      gCGAATGCGCTGGCAGCCGCTGCGCTCTCCATGTCCGTGGGCGCAACGCTTGATGCTATCAAAGCGGGGCTGGCAAATCTGAAAGCTGTTCCAGGCCGTCTGTTCCCCATCCAACTGGCAGAAAACCAGTTGCTACTcg                                                                                                                                   <  2:577215/139‑1 (MQ=255)  cGAATGCGCTGGCAGCCGCTGCGCTCTCCATGTCCGTGGGCGCAACGCTTGATGCTATCAAAGCGGGGCTGGCAAATCTGAAAGCTGTTCCAGGCCGTCTGTTCCCCATCCAACTGGCAGAAAACCAGTTGCTACTcga                                                                                                                                  >  2:918947/1‑139 (MQ=255)   gAATGCCCTGGCAGCCGCTGCGCTCTCCATGTCCGTGGGCGCAACGCTTTATGCTATCAAAGCGGGGCTGGCAAATCTGAAAGCTGTTCCAGGCCGTCTGTTCCCCATCCAACTGGCAGAAAACCAGTTGCTACTcgac                                                                                                                                 <  2:912955/139‑1 (MQ=255)    aaTGCGCTGGCAGCCGCTGCGCTCTCCATGTCCGTGGGCGCAACGCTTGATGCTATCAAAGCGGGGCTGGCAAATCTGAAAGCTGTTCCAGGCCGTCTGTTCCCCATCCAACTGGCAGAAAACCAGTTGCTACTcgacg                                                                                                                                <  1:909934/139‑1 (MQ=255)    aaTGCGCTGGCAGCCGCTGCGCTCTCCATGTCCGTGGGCGCAACGCTTGATGCTATCAAAGCGGGGCTGGCAAATCTGAAAGCTGTTCCAGGCCGTCTGTTCCCCATCCAACTGGCAGAAAACCAGTTGCTACTcgacg                                                                                                                                >  1:236948/1‑139 (MQ=255)       gcgcTGGTAGCCGCTGCGCTCTCCATGTCCGTGGGCGCAACGCTTGATGCTATCAAAGCGGGGCTGGCAAATCTGAAAGCTGTTCCAGGCCGTCTGTTCCCCATCCAACTGGCAGAAAACCAGTTGCTACTCGACGACt                                                                                                                             >  2:386923/1‑139 (MQ=255)       gcgcTGGCAGCCGCTGCGCTCTCCATGTCCGTGGGCGCAACGCTTGATGCTATCAAAGCGGGGCTGGCAAATCTGAAAGCTGTTCCAGGCCGTCTGTTCCCCATCCAACTGGCAGAAAACCAGTTGCTACTCGACGACt                                                                                                                             >  2:370992/1‑139 (MQ=255)       gcgcTGGCAGCCGCTGCGCTCTCCATGTCCGTGGGCGCAACGCTTGATGCTATCAAAGCGGGGCTGGCAAATCTGAAAGCTGTTCCAGGCCGTCTGTTCCCCATCCAACTGGCAGAAAACCAGTTGCTACTCGACGACt                                                                                                                             >  2:476885/1‑139 (MQ=255)         gcTGGCAGCCGCTGCGCTCTCCATGTCCGTGGGCGCAACGCTTGATGCTATCAAAGCGGGGCTGGCAAATCTGAAAGCTGTTCCAGGCCGTCTGTTCCCCATCCAACTGGCAGAAAACCAGTTGCTACTCGACGACTcc                                                                                                                           >  1:545115/1‑139 (MQ=255)                gCCGCTGCGCTCTCCATGTCCGTGGGCGCAACGCTTGATGCTATCAAAGCGGGGCTGGCAAATCTGAAAGCTGTTCCAGGCCGTCTGTTCCCCATCCAACTGGCAGAAAACCAGTTGCTACTCGACGACTCCTACAACg                                                                                                                    <  2:789867/139‑1 (MQ=255)                    cTGCGCTCTCCATGTCCGTGGGCGCAACGCTTGATGCTATCAAAGCGGGGCTGGCAAATCTGAAAGCTGTTCCAGGCCGTCTGTTCCCCATCCAACTGGCAGAAAACCAGTTGCTACTCGACGACTCCTACAACGCCaa                                                                                                                >  1:287102/1‑139 (MQ=255)                    cTGCGCTCTCCATGTCCGTGGGCGCAACGCTTGATGCTATCAAAGCGGGGCTGGCAAATCTGAAAGCTGTTCCAGGCCGTCTGTTCCCCATCCAACTGGCAGAAAACCAGTTGCTACTCGACGACTCCTACAACGCCaa                                                                                                                >  1:904160/1‑139 (MQ=255)                      gcgcTCTCCATGTCCGTGGGCGCAACGCTTGATGCTATCAAAGCGGGGCTGGCAAATCTGAAAGCTGTTCCAGGCCGTCTGTTCCCCATCCAACTGGCAGAAAACCAGTTGCTACTCGACGACTCCTACAACGCCAATg                                                                                                              <  1:332872/139‑1 (MQ=255)                      gcgcTCTCCATGTCCGTGGGCGCAACGCTTGATGCTATCAAAGCGGGGCTGGCAAATCTGAAAGCTGTTCCAGGCCGTCTGTTCCCCATCCAACTGGCAGAAAACCAGTTGCTACTCGACGACTCCTACAACGCCAATg                                                                                                              <  1:201090/139‑1 (MQ=255)                                      tGGGCGCAACGCTTGATGCTATCAAAGCGGGGCTGGCAAATCTGAAAGCTGTTCCAGGCCGTCTGTTCCCCATCCAACTGGCAGAAAACCAGTTGCTACTCGACGACTCCTACAACGCCAATGTCGGTTCAATGACTgc                                                                                              >  2:224637/1‑139 (MQ=255)                                      tGGGCGCAACGCTTGATGCTATCAAAGCGGGGCTGGCAAATCTGAAAGCTGTTCCAGGCCGTCTGTTCCCCATCCAACTGGCAGAAAACCAGTTGCTACTCGACGACTCCTACAACGCCAATGTCGGTTCAATGACTgc                                                                                              >  1:59147/1‑139 (MQ=255)                                       gggCGCAACGCTTGATGCTATCAAAGCGGGGCTGGCAAATCTGAAAGCTGTTCCAGGCCGTCTGTTCCCCATCCAACTGGCAGAAAACCAGTTGCTACTCGACGACTCCTACAACGCCAATGTCGGTTCAATGACTgca                                                                                             <  2:374133/139‑1 (MQ=255)                                                               aGCGGGGCTGGCAAATCTGAAAGCTGTTCCAGGCCGTCTGTTCCCCATCCAACTGGCAGAAAACCAGTTGCTACTCgacgac                                                                                                                              >  2:20257/1‑82 (MQ=255)                                                               aGCGGGGCTGGCAAATCTGAAAGCTGTTCCAGGCCGTCTGTTCCCCATCCAACTGGCAGAAAACCAGTTGCTACTCgacgac                                                                                                                              <  1:20257/82‑1 (MQ=255)                                                               aGCGGGGCTGGCAAATCTGAAAGCTGTTCCAGGCCGTCTGTTCCCCATCCAACTGGCAGAAAACCAGTTGCTACTCGACGACTCCTACAACGCCAATGTCGGTTCAATGACTGCAGCAGTCCAGGTACTGGCTGAAATg                                                                     <  1:411385/139‑1 (MQ=255)                                                                     gCTGGCAAATCTGAAAGCTGTTCCAGGCCGTCTGTTCCCCATCCAACTGGCAGAAAACCAGTTGCTACTCGACGACTCCTACAACGCCAATGTCGGTTCAATTACTGCAGCAGTCCAGGTACTGGCTGAAATGCCGGGc                                                               >  2:694679/1‑139 (MQ=255)                                                                     gCTGGCAAATCTGAAAGCTGTTCCAGGCCGTCTGTTCCCCATCCAACTGGCAGAAAACCAGTTGCTACTCGACGACTCCTACAACGCCAATGTCGGTTCAATGACTgcagc                                                                                           <  1:12209/111‑1 (MQ=255)                                                                     gCTGGCAAATCTGAAAGCTGTTCCAGGCCGTCTGTTCCCCATCCAACTGGCAGAAAACCAGTTGCTACTCGACGACTCCTACAACGCCAATGTCGGTTCAATGACTgcagc                                                                                           >  2:12209/1‑111 (MQ=255)                                                                         gCAAATCTGAAAGCTGTTCCAGGCCGTCTGTTCCCCATCCAACTGGCAGAAAACCAGTTGCTACTCGACGACTCCTACAACGCCAATGTCGGTTCAATGACTgcag                                                                                            <  2:447607/106‑1 (MQ=255)                                                                         gCAAATCTGAAAGCTGTTCCAGGCCGTCTGTTCCCCATCCAACTGGCAGAAAACCAGTTGCTACTCGACGACTCCTACAACGCCAATGTCGGTTCAATGACTgcag                                                                                            >  1:447607/1‑106 (MQ=255)                                                                          cAAATCTGAAAGCTGTTCCAGGCCGTCTGTTCCCCATCCAACTGGCAGAAAACCAGTTGCTACTCGACGACTCCTACAACGCCAATGTCGGTTCAATGACTGCAGCAGTCCAGGTACTGGCTGAAATGCCGGGCTACcg                                                          <  1:476885/139‑1 (MQ=255)                                                                              tCTGAAAGCTGTTCCAGGCCGTCTGTTCCCCATCCAACTGGCAGAAAACCAGTTGCTACTCGACGACTCCTACAACGCCAATg                                                                                                              <  1:1192658/83‑1 (MQ=255)                                                                              tCTGAAAGCTGTTCCAGGCCGTCTGTTCCCCATCCAACTGGCAGAAAACCAGTTGCTACTCGACGACTCCTACAACGCCAATg                                                                                                              >  2:1192658/1‑83 (MQ=255)                                                                                  aaaGCTGTTCCAGGCCGTCTGTTCCCCATCCAACTGGCAGAAAACCAGTTGCTACTCGACGACTCCTACAACGCCAATGTCGGTTCAATGACTGCAGCAGTCCAGGTACTGGCTGAAATGCCGGGCTACCGCGTGCtgg                                                  >  2:1198074/1‑139 (MQ=255)                                                                                    aGCTGTTCCAGGCCGTCTGTTCCCCATCCAACTGGCAGAAAACCAGTTGCTACTCGACGACTCCTACAACGCCAATGTCGGTTCAATGACTGCAGCAGTCCAGGTACTGGCTGAAATGCCGGGCTACCGCGTGCtggtg                                                <  2:796397/139‑1 (MQ=255)                                                                                       tGTTCCAGGCCGTCTGTTCCCCATCCAACTGGCAGAAAACCAGTTGCTACTCGACGACTCCTACAACGCCAATGTCGGTTCAATGACTGCAGCAGTCCAGGTACTGGCTGAAATGCCGGGCTACCGCGTGCtggtggtg                                             >  1:509289/1‑139 (MQ=255)                                                                                         ttCCAGGCCGTCTGTTCCCCATCCAACTGGCAGAAAACCAGTTGCTACTCGACGACTCCTACAACGCCAATGTCGGTTCAATGACTGCAGCAGTCCAGGTACTGGCTGAAATGCCGGGCTACCGCGTGCTGGTGGTggg                                           >  2:1085207/1‑139 (MQ=255)                                                                                         ttCCAGGCCGTCTGTTCCCCATCCAACTGGCAGAAAACCAGTTGCTACTCGACGACTCCTACAACGCCAATGTCGGTTCAATGACTGCAGCAGTCCAGGTACTGGCTGAAATGCCGGGCTACCGCGTGCTGGTGGTggg                                           <  1:370992/139‑1 (MQ=255)                                                                                            cAGGCCGTCTGTTCCCCATCCAACTGGCAGAAAACCAGTTGCTACTCGACGACTCCTACAACGCCAATGTCGGTTCAATGACTGCAGCAGTCCAGGTACTGGCTGAAATGCCGGGCTACCGCGTGCTGGTGGTGGGCGa                                        <  1:990948/139‑1 (MQ=255)                                                                                            cAGGCCGTCTGTTCCCCATCCAACTGGCAGAAAACCAGTTGCTACTCGACGACTCCTACAACGCCAATGTCGGTTCAATGACTGCAGCAGTCCAGGTACTGGCTGAAATGCCGGGCTACCGCGTGCTGGTGGTGGGCGa                                        <  2:317155/139‑1 (MQ=255)                                                                                              ggCCGTCTGTTCCCCATCCAACTGGCAGAAAACCAGTTGCTACTCGACGACTCCTACAACGCCAATGTCGGTTCAATGACTGCAGCAGTCCAGGTACTGGCTGAAATGCCGGGCTACCGCGTGCTGGTGGTGGGCGata                                      <  2:545115/139‑1 (MQ=255)                                                                                                 cGTCTGTTCCCCATCCAACTGGCAGAAAACCAGTTGCTACTCGACGACTCCTACAACGCCAATGTCGGTTCAATGACTGCAGCAGTCCAGGTACTGGCTGAAATGCCGGGCTACCGCGTGCTGGTGGTGGGCGATATgg                                   >  1:667832/1‑139 (MQ=255)                                                                                                   tCTGTTCCCCATCCAACTGGCAGAAAACCAGTTGCTACTCGACGACTCCTACAACGCCAATGTCGGt                                                                                                         >  2:667720/1‑67 (MQ=255)                                                                                                   tCTGTTCCCCATCCAACTGGCAGAAAACCAGTTGCTACTCGACGACTCCTACAACGCCAATGTCGGt                                                                                                         <  1:667720/67‑1 (MQ=255)                                                                                                   tCTGTTCCCCATCCAACTGGCAGAAAACCAGTTGCTACTCGACGACTCCTACAACGCCAATGTCGGTTCAATGACTGCAGCAGTCCAGGTACTGGCTGAAATGCCGGGCTACCGCGTGCTGGTGGTGGGCGATATGGCg                                 >  2:1117218/1‑139 (MQ=255)                                                                                                              tCCAACTGGCAGAAAACCAGTTGCTACTCGACGACTCCTACAACGCCAATGTCGGTTCAATGACTGCAGCAGTCCAGGTACTGGCTGAAATGCCGGGCTACCGCGTGCTGGTGGTGGGCGATATGGCGGAACTGGgcgc                      >  1:464372/1‑139 (MQ=255)                                                                                                                    tGGCAGAAAACCAGTTGCTACTCGACGACTCCTACAACGCCAATGTCGGTTCAATG‑CTGCAGCAGTCCAGGTACTGGCTGAAATGCCGGGCTACCGCGTGCTGGTGGTGGGCGATATGGCGGAACTGGgcg                       <  2:1183544/131‑1 (MQ=255)                                                                                                                    tGGCAGAAAACCAGTTGCTACTCGACGACTCCTACAACGCCAATGTCGGTTCAATG‑CTGCAGCAGTCCAGGTACTGGCTGAAATGCCGGGCTACCGCGTGCTGGTGGTGGGCGATATGGCGGAACTGGgcg                       >  1:1183544/1‑131 (MQ=255)                                                                                                                          aaaaCCAGTTGCTACTCGACGACTCCTACAACGCCAATGTCGGTTCAATGACTGCAGCAGTCCAGGTACTGGCTGAAATGCCGGGCTACCGCGTGCTGGTGGTGGGCGATATGGCGGAACTGGGCGCTGAAAGCGAAGc          <  1:694679/139‑1 (MQ=255)                                                                                                                          aaaaCCAGTTGCTACTCGACGACTCCTACAACGCCAATGTCGGTTCAATGACTGCAGCAGTCCAGGTACTGGCTGAAATGCCGGGCTACCGCGTGCTGGTGGTGGGCGATATGGCGGAACTGGGCGCTGAAAGCGAAGc          <  2:81078/139‑1 (MQ=255)                                                                                                                             aCCAGTTGCTACTCGACGACTCCTACAACGCCAATGTCGGTTCAATGACTGCAGCAGTCCAGGTACTGGCTGAAATGCCGGGCTACCGCGTGCTGGTGGTGGGCGATATGGCGGAACTGGGCGCTGAAAGCGAAGCCTg       <  1:136773/139‑1 (MQ=255)                                                                                                                                  ttGCTACTCGACGACTCCTACAACGCCAATGTCGGTTCAATGACTGCAGCAGTCCAGGTACTGGCTGAAATGCCGGGCTACCGCGTGCTGGTGGTGGGCGATATGGCGGAACTGGGCGCTGAAAGCGAAGCCTGCCATg  <  1:1117218/139‑1 (MQ=255)                                                                                                                                       |                                                                                                                                      GCGAATGCGCTGGCAGCCGCTGCGCTCTCCATGTCCGTGGGCGCAACGCTTGATGCTATCAAAGCGGGGCTGGCAAATCTGAAAGCTGTTCCAGGCCGTCTGTTCCCCATCCAACTGGCAGAAAACCAGTTGCTGCTCGACGACTCCTACAACGCCAATGTCGGTTCAATGACTGCAGCAGTCCAGGTACTGGCTGAAATGCCGGGCTACCGCGTGCTGGTGGTGGGCGATATGGCGGAACTGGGCGCTGAAAGCGAAGCCTGCCATG  >  NC_000913/95499‑95766

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑