| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,511,099 | G→A | R366H (CGC→CAC) | ydcR → | putative DNA‑binding transcriptional regulator and putative aminotransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,511,099 | 0 | G | A | 94.1% | 154.1 / 1.6 | 51 | R366H (CGC→CAC) | ydcR | putative DNA‑binding transcriptional regulator and putative aminotransferase |
| Reads supporting (aligned to +/- strand): ref base G (1/2); new base A (26/22); total (27/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.95e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.34e-01 | |||||||||||
GGTTGGGTCGCCGCCGGAAAACATGCACGTAAAATTCAACGCTTGCAGTTGATGAGTACGCTTTCCACCAGCTCACCGATGCAACTTGCGCTGGTGGATTACCTTTCCACGCGCCGATACGACGCCCATCTTCGTCGCCTGCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGAAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGA > NC_000913/1510963‑1511213 | ggttggGTCGCCGCCGGAAAACATGCACGTAAAATTCAACGCTTGCAGTTGATGAGTACGCTTTCCACCAGCTCACCGATGCAACTTGCGCTGGTGGATTACCTTTCCACGCGCCGATACGACGCCCATCTTCGTCAcc > 1:561000/1‑139 (MQ=255) gTCGCCGCCGGAAAACATGCACGTAAAATTCAACGCTTGCAGTTGATGAGTACGCTTTCCACCAGCTCACCGATGCAACTTGCGCTGGTGGATTACCTTTCCACGCACCGATACGACGCCCATCTTCGTCACCTGCGTc > 2:1096370/1‑139 (MQ=255) cgccgGAAAACATGCACGTAAAATTCAACGCTTGCAGTTGATGAGTACGCTTTCCACCAGCTCACCGATGCAACTTGCGCTGGTGGATTACCTTTCCACGCGCCGATACGACGCCCATCTTCGTCACCTGCGTCGCCAg > 2:1054748/1‑139 (MQ=255) gAAAACATGCACGTAAAATTCAACGCTTGCAGTTGATGAGTACGCTTTCCACCAGCTCACCGATGCAACTTGCGCTGGTGGATTACCTTTCCACGCACCGATACGACGCCCATCTTCGTCACCTGCGTCGCCAGCTTGc < 1:1096370/139‑1 (MQ=255) aaaCATGCACGTAAAATTCAACGCTTGCAGTTGATGAGTACGCTTTCCACCAGCTCACCGATGCAACTTGCGCTGGTGGATTACCTTTCCACGCGCCGATACGACGCCCATCTTCGTCACCTGCGTCGCCAGCTTGCgg < 2:1082041/139‑1 (MQ=255) aaaCATGCACGTAAAATTCAACGCTTGCAGTTGATGAGTACGCTTTCCACCAGCTCACCGATGCAACTTGCGCTGGTGGATTACCTTTCCACGCGCCGATACGACGCCCATCTTCGTCACCTGCGTCGCCAGCTTGCgg < 1:1054748/139‑1 (MQ=255) cACGTAAAATTCAACGCTTGCAGTTGATGAGTACGCTTTCCACCAGCTCACCGATGCAACTTGCGCTGGTGGATTACCTTTCCACGCGCCGATACGACGCCCATCTTCGTCACCTGCGTCGCCAGCTTGCGGAACGTaa > 1:91144/1‑139 (MQ=255) aaaTTCAACGCTTGCAGTTGATGAGTACGCTTTCCACCAGCTCACCGATGCAACTTGCGCTGGTGGATTACCTTTCCACGCGCCGATACGACGCCCATCTTCGTCACCTGCGTCGCCAGCTTGCGGAACGTAAACAACg > 1:131424/1‑139 (MQ=255) cGCTTGCAGTTGATGAGTACGCTTTCCACCAGCTCACCGATGCAACTTGCGCTGGTGGATTACCTTTCCACGCGCCGATACGACGCCCATCTTCGTCACCTGCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTggc > 2:517946/1‑139 (MQ=255) cGCTTGCAGTTGATGAGTACGCTTTCCACCAGCTCACCGATGCAACTTGCGCTGGTGGATTACCTTTCCACGCGCCGATACGACGCCCATCTTCGTCACCTGCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTggc < 1:434944/139‑1 (MQ=255) ttGCAGTTGATGAGTACGCTTTCCACCAGCTCACCGATGCAACTTGCGCTGGTGGATTACCTTTCCACGCGCCGATACGACGCCCATCTTCGTCACCTGCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTggcagg > 2:689499/1‑139 (MQ=255) tGCAGTTGATGAGTACGCTTTCCACCAGCTCACCGATGCAACTTGCGCTGGTGGATTACCTTTCCACGCGCCGATACGACGCCCATCTTCGTCACCTGCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTggcaggc > 2:12587/1‑139 (MQ=255) gCAGTTGATGAGTACGCTTTCCACCAGCTCACCGATGCAACTTGCGCTGGTGGATTACCTTTCCACGCGCCGATACGACGCCCATCTTCGTCACCTGCGTCGCCAGCTTGCGGAACGTa > 2:371451/1‑119 (MQ=255) gCAGTTGATGAGTACGCTTTCCACCAGCTCACCGATGCAACTTGCGCTGGTGGATTACCTTTCCACGCGCCGATACGACGCCCATCTTCGTCACCTGCGTCGCCAGCTTGCGGAACGTa < 1:371451/119‑1 (MQ=255) aGTTGATGAGTACGCTTTCCACCAGCTCACCGATGCAACTTGCGCTGGTGGATTACCTTTCCACGCGCCGATACGACGCCCATCTTCGTCGCCTGCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTGGCAGGCAct < 2:982165/139‑1 (MQ=255) aGTTGATGAGTACGCTTTCCACCAGCTCACCGATGCAACTTGCGCTGGTGGATTACCTTTCCACGCGCCGATACGACGCCCATCTTCGTCACCTGCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTGGCAGGCAct < 1:1120043/139‑1 (MQ=255) gatgaGTACGCTTTCCACCAGCTCACCGATGCAACTTGCGCTGGTGGATTACCTTTCCACGCGCCGATACGACGCCCATCTTCGTCACCTGCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTGGCAGGCActgctg < 1:916584/139‑1 (MQ=255) tgaGTACGCTTTCCACCAGCTCACCGATGCAACTTGCGCTGGTGGATTACCTTTCCACGCGCCGATACGACGCCCATCTTCGTCACCTGCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCg > 1:221374/1‑139 (MQ=255) tACGCTTTCCACCAGCTCACCGATGCAACTTGCGCTGGTGGATTACCTTTCCACGCGCCGATACGACGCCCATCTTCGTCACCTGCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTAt > 1:1196482/1‑139 (MQ=255) cGCTTTCCACCAGCTCACCGATGCAACTTGCGCTGGTGGATTACCTTTCCACGCGCCGATACGACGCCCATCTTCGTCACCTGCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTa > 2:246214/1‑136 (MQ=255) cGCTTTCCACCAGCTCACCGATGCAACTTGCGCTGGTGGATTACCTTTCCACGCGCCGATACGACGCCCATCTTCGTCACCTGCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTa < 1:246214/136‑1 (MQ=255) tCCACCAGCTCACCGATGCAACTTGCGCTGGTGGATTACCTTTCCACGCGCCGATACGACGCCCATCTTCGTCACCTGCGTCGCCAGCTTGCGGAACGTAAACAACGTGcc > 2:569958/1‑111 (MQ=255) tCCACCAGCTCACCGATGCAACTTGCGCTGGTGGATTACCTTTCCACGCGCCGATACGACGCCCATCTTCGTCACCTGCGTCGCCAGCTTGCGGAACGTAAACAACGTGcc < 1:569958/111‑1 (MQ=255) tCCACCAGCTCACCGATGCAACTTGCGCTGGTGGATTACCTTTCCACGCGCCGATACGACGCCCATCTTCGTCACCTGCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATctgcctg > 1:1109262/1‑139 (MQ=255) cTCACCGATGCAACTTGCGCTGGTGGATTACCTTTCCACGCGCCGATACGACGCCCATCTTCGTCACCTGCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTg > 1:331387/1‑139 (MQ=255) cACCGATGCAACTTGCGCTGGTGGATTACCTTTCCACGCGCCGATACGACGCCCATCTTCGTCACCTGCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGaa > 2:1056510/1‑139 (MQ=255) cACCGATGCAACTTGCGCTGGTGGATTACCTTTCCACGAGCCGATACGACGCCCACCTTCGTCACCTGCGGCGCCAGCTTGCGGAACGTACACAACGTGCCTGGCAGGCACTGCTGCGTTATCTACCTGCGGAAGTGaa > 2:987525/1‑139 (MQ=255) cGATGCAACTTGCGCTGGTGGATTACCTTTCCACGCGCCGATACGACGCCCATCTTCGTCACCTGCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGAAAAt < 2:1196482/139‑1 (MQ=255) cGATGCAACTTGCGCTGGTGGATTACCTTTCCACGCGCCGATACGACGCCCATCTTCGTCACCTGCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGAAAAt < 2:91144/139‑1 (MQ=255) cGATGCAACTTGCGCTGGTGGATTACCTTTCCACGCGCCGATACGACGCCCATCTTCGTCACCTGCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGAAAAt < 1:806304/139‑1 (MQ=255) gCAACTTGCGCTGGTGGATTACCTTTCCACGCGCCGATACGACGCCCATCTTCGTCACCTGCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGAAAATtcat < 1:1105599/139‑1 (MQ=255) gCAACTTGCGCTGGTGGATTACCTTTCCACGCGCCGATACGACGCCCATCTTCGTCACCTGCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGAAAATtcat < 1:517946/139‑1 (MQ=255) gCAACTTGCGCTGGTGGATTACCTTTCCACGCGCCGATACGACGCCCATCTTCGTCACCTGCGTCGCCAACTTGCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGAAAATtcat < 2:337215/139‑1 (MQ=255) cAACTTGCGCTGGTGGATTACCTTTCCACGCGCCGATACGACGCCCATCTTCGTCACCTGCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGAAAATtcatc > 2:919431/1‑139 (MQ=255) tGCGCTGGTGGATTACCTTTCCACGCGCCGATACGACGCCCATCTTCGTCGCCTGCGTCGCCAGCTTGCGGaa > 2:694490/1‑73 (MQ=255) tGCGCTGGTGGATTACCTTTCCACGCGCCGATACGACGCCCATCTTCGTCGCCTGCGTCGCCAGCTTGCGGaa < 1:694490/73‑1 (MQ=255) cgcTGGTGGATTACCTTTCCACGCGCCGATACGACGCCCATCTTCGTCACCTGCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGAAAATTCATCATAATGa < 2:1109262/139‑1 (MQ=255) cTGGTGGATTACCTTTCCACGCGCCGATACGACGCCCATCTTCGTCACCTGCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGAAAATTCATCATAATGTCa < 1:919431/139‑1 (MQ=255) tggtggATTACCTTTCCACGCGCCGATACGACGCCCATCTTCGTCACCTGCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGAAAATTCATCATAATGACAg < 2:1125893/139‑1 (MQ=255) tggATTACCTTTCCACGCGCCGATACGACGCCCATCTTCGTCACCTGCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGAAAATTCATCATAATGACAGTgg > 1:793232/1‑139 (MQ=255) ttACCTTTCCACGCGCCGATACGACGCCCATCTTCGTCACCTGCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGt > 2:502825/1‑111 (MQ=255) ttACCTTTCCACGCGCCGATACGACGCCCATCTTCGTCACCTGCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGt < 1:502825/111‑1 (MQ=255) ttACCTTTCCACGCGCCGATACGACGCCCATCTTCGTCACCTGCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGAAAATTCATCATAATGACAGTGGTTAc > 2:969795/1‑139 (MQ=255) ccACGCGCCGATACGACGCCCATCTTCGTCACCTGCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGAAAATTCATCATAATGACAGTGGTTACTTTCTCTg > 1:23305/1‑139 (MQ=255) cgcCGATACGACGCCCATCTTCGTCACCTGCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCg > 2:877536/1‑93 (MQ=255) cgcCGATACGACGCCCATCTTCGTCACCTGCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCg < 1:877536/93‑1 (MQ=255) gcCGATACGACGCCCATCTTCGTCACCTGCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGAAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGa > 1:565280/1‑139 (MQ=255) ccGATACGACGCCCATCTTCGTCACCTGCGTCGCCAGCTTGc > 2:461207/1‑42 (MQ=255) ccGATACGACGCCCATCTTCGTCACCTGCGTCGCCAGCTTGc < 1:461207/42‑1 (MQ=255) cATCTTCGTCACCTGCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTggcagg > 2:429954/1‑55 (MQ=255) cATCTTCGTCACCTGCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTggcagg < 1:429954/55‑1 (MQ=255) | GGTTGGGTCGCCGCCGGAAAACATGCACGTAAAATTCAACGCTTGCAGTTGATGAGTACGCTTTCCACCAGCTCACCGATGCAACTTGCGCTGGTGGATTACCTTTCCACGCGCCGATACGACGCCCATCTTCGTCGCCTGCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGAAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGA > NC_000913/1510963‑1511213 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |