Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,866,995 C→T Q30* (CAA→TAA)  yeaG → protein kinase, endogenous substrate unidentified, autokinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,866,9950CT100.0% 86.6 / NA 26Q30* (CAA→TAA) yeaGprotein kinase, endogenous substrate unidentified, autokinase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/10);  total (16/10)

ATGAACGGCTCTTAACCTGTGCTAAAAAACGAAAGGACGGCATACCATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTACCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATACAGCCCAGGAACCCAGACTTTCTCGACTCTTTTCTAACCGGGTCATTGCACGTTATCCGGC  >  NC_000913/1866862‑1867128
                                                                                                                                     |                                                                                                                                     
atGAACGGCTCTTAACCTGTGCTAAAAAACGAAAGGACGGCATACCATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTACCACTTGTCGGTAAGAt                                                                                                                                  >  1:476935/1‑139 (MQ=255)
    aCGGCTCTTAACCTGTGCTAAAAAACGAAAGGACGGCATACCATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTACCACTTGTCGGTAAGATCGCa                                                                                                                              <  2:476935/139‑1 (MQ=255)
    aCGGCTCTTAACCTGTGCTAAAAAACGAAAGGACGGCATACCATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTACCACTTGTCGGTAAGATCGCa                                                                                                                              <  2:631293/139‑1 (MQ=255)
        ctctTAACCTGTGCTAAAAAACGAAAGGACGGCATACCATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTACCACTTGTCGGTAAGATCGCAGTGc                                                                                                                          >  2:535171/1‑139 (MQ=255)
        ctctTAACCTGTGCTAAAAAACGAAAGGACGGCATACCATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTACCACTTGTCGGTAAGATCGCAGTGc                                                                                                                          >  2:74745/1‑139 (MQ=255)
                           aaCGAAAGGACGGCATACCATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTACCACTTGTCGGTAAGATCGCAGTGCTTATGCCAACGCGGCTGAg                                                                                                       <  2:983347/139‑1 (MQ=255)
                                        cATACCATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTACCACTTGTCGGTAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATgg                                                                                          >  1:1190499/1‑139 (MQ=255)
                                                           aTCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTACCACTTGTCGGTAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGt                                                                                  >  2:869268/1‑128 (MQ=255)
                                                           aTCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTACCACTTGTCGGTAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGt                                                                                  <  1:869268/128‑1 (MQ=255)
                                                           aTCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTACCACTTGTCGGTAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTc                                                                         <  2:612068/137‑1 (MQ=255)
                                                           aTCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTACCACTTGTCGGTAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTc                                                                         >  1:612068/1‑137 (MQ=255)
                                                             cACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTACCACTTGTCGGTAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATgg                                                                     >  1:157399/1‑139 (MQ=255)
                                                                   cGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTACCACTTGTCGGTAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATa                                                               <  1:1070284/139‑1 (MQ=255)
                                                                                   cTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTACCACTTGTCGGTAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTc                                                                         >  2:193132/1‑113 (MQ=255)
                                                                                   cTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTACCACTTGTCGGTAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTc                                                                         <  1:193132/113‑1 (MQ=255)
                                                                                                     tCACACTGCAGGAGTTTCTTACCACTTGTCGGTAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATACAGCCCAGGAACCCAGACTTTCTCGACTCTTTTc                             >  2:963301/1‑139 (MQ=255)
                                                                                                            gCAGGAGTTTCTTACCACTTGTCGGTAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGa                                                                                >  1:1118851/1‑81 (MQ=255)
                                                                                                            gCAGGAGTTTCTTACCACTTGTCGGTAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGa                                                                                <  2:1118851/81‑1 (MQ=255)
                                                                                                              aGGAGTTTCTTACCACTTGTCGGTAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATACAGCCCAGGAACCCAGACTTTCTCGACTCTTTTCTAACCGGGt                    >  2:849678/1‑139 (MQ=255)
                                                                                                                taGTTTCTTACCACTTGTCGGTAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATa                                                               >  2:755233/2‑94 (MQ=255)
                                                                                                                gAGTTTCTTACCACTTGTCGGTAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGTGCCTGTCATGGTCGATa                                                               <  1:755233/94‑1 (MQ=255)
                                                                                                                             ctagacGGTAATATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTc                                                                         >  2:701281/6‑71 (MQ=255)
                                                                                                                             cTTGTCGGTAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTc                                                                         <  1:701281/71‑1 (MQ=255)
                                                                                                                               tGTCGGTAAGATCGCAGTGCTTATGCCAACGCGGCTGAGTGACTATTGATGCCTATCGGTGAACCTGTCATGGTCGATCCAGCCCAGGAACCCAGACTTACTCGCCTCCTTTCTAACCGGGTCATTGCCCGTTATCCgg   >  2:744935/1‑139 (MQ=255)
                                                                                                                                gTCGGTAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATACAGCCCAGGAACCCAGACTTTCTCGACTCTTTTCTAACCGGGTCATTGCACGTTATCCGGc  >  2:463463/1‑139 (MQ=255)
                                                                                                                                gTCGGTAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATACAGCCCAGGAACCCAGACTTTCTCGACTCTTTTCTAACCGGGTCATTGCACGTTATCCGGc  >  1:865155/1‑139 (MQ=255)
                                                                                                                                     |                                                                                                                                     
ATGAACGGCTCTTAACCTGTGCTAAAAAACGAAAGGACGGCATACCATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTACCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATACAGCCCAGGAACCCAGACTTTCTCGACTCTTTTCTAACCGGGTCATTGCACGTTATCCGGC  >  NC_000913/1866862‑1867128

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: