BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,981,182	C→T	R144Q (CGG→CAG)	otsA ←	trehalose‑6‑phosphate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,981,182	0	C	T	100.0%	126.6 / NA	37	R144Q (CGG→CAG)	otsA	trehalose‑6‑phosphate synthase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (17/20); total (17/20)

TCTGGAAACCCAGCAAATCATAATCACAAAGCTGTTCAAGCAAGGTGTCATATGTCGGCAGCGCGTTGAAGATTTCCGGTGTCGGGAAAGGAATATGC‑‑‑‑AGAAAGAAACCAATGCGATTATTCACTCCCCGTTTGCGTAATTCATGCGCAAATGGCAACAGGTGATAATCGTGGATCCAGATAATGTCATCGTCTTGCAACAGCGGCAGTAATTTATCTGCCAGCAACGCATTTACGCGTAGATAGCCGTCCCAGGCAG  >  NC_000913/1981054‑1981311

tctgGAAACCCAGCAAATCATAATCACAAAGCTGTTCAAGCAAGGTGTCATATGTCGGCAGCGCGTTGAAGATTTCCGGTGTCGGGAAAGGAATATGC‑‑‑‑AGAAAGAAACCAATGCGATTATTCACTCCCTGTTTGCGTaa                                                                                                                         >  1:986518/1‑139 (MQ=255)

          cAGCAAATCATAATCACAAAGCTGTTCAAGCAAGGTGTCATATGTCGGCAGCGCGTTGAAGATTTCCGGTGTCGGGAAAGGAATATGC‑‑‑‑AGAAAGAAACCAATGCGATTATTCACTCCCTGTTTGCGTAATTCATGCGCa                                                                                                               >  2:850257/1‑139 (MQ=255)

                  cATAATCACAAAGCTGTTCAAGCAAGGTGTCATATGTCGGCAGCGCGTTGAAGATTTCCGGTGTCGGGAAAGGAATATGC‑‑‑‑AGAAAGAAACCAATGCGATTATTCACTCCCTGTTTGCGTAATTCATGCGCAAATGGCaa                                                                                                       <  2:986518/139‑1 (MQ=255)

                  cATAATCACAAAGCTGTTCAAGCAAGGTGTCATATGTCGGCAGCGCGTTGAAGATTTCCGGTGTCGGGAAAGGAATATGC‑‑‑‑AGAAAGAAACCAATGCGATTATTCACTCCCTGTTTGCGTAATTCATGCGCAAATGGCaa                                                                                                       <  1:722747/139‑1 (MQ=255)

                   aTAATCACAAAGCTGTTCAAGCAAGGTGTCATATGTCGGCAGCGCGTTGAAGATTTCCGGTGTCGGGAAAGGAATATGC‑‑‑‑AGAAAGAAACCAATGCGATTATTCACTCCCTGTTTGCGTAATTCATGCGCAAATGGCaa                                                                                                       >  2:1194811/1‑138 (MQ=255)

                   aTAATCACAAAGCTGTTCAAGCAAGGTGTCATATGTCGGCAGCGCGTTGAAGATTTCCGGTGTCGGGAAAGGAATATGC‑‑‑‑AGAAAGAAACCAATGCGATTATTCACTCCCTGTTTGCGTAATTCATGCGCAAATGGCaa                                                                                                       <  1:1194811/138‑1 (MQ=255)

                           aaaGCTGTTCAAGCAAGGTGTCATATGTCGGCAGCGCGTTGAAGATTTCCGGTGTCGGGAAAGGAATATGC‑‑‑‑AGAAAGAAACCAATGCGATTATTCACTCCCTGTTTGCGTAATTCATGCGCAAATGGCAACAGGTGATa                                                                                              >  1:172081/1‑139 (MQ=255)

                            aaGCTGTTCAAGCAAGGTGTCATATGTCGGCAGCGCGTTGAAGATTTCCGGTGTCGGGAAAGGAATATGC‑‑‑‑AGAAAGAAACCAATGCGATTATTCACTCCCTGTTTGCGTAATTCATGCGCAAATGGCAACAGGTGATaa                                                                                             >  1:911018/1‑139 (MQ=255)

                             aGCTGTTCAAGCAAGGTGTCATATGTCGGCAGCGCGTTGAAGATTTCCGGTGTCGGGAAAGGAATATGC‑‑‑‑AGAAAGAAACCAATGCGATTATTCACTCCCTGTTTGCGTAATTCATGCGCAAATGGCAACAGGTGATAAt                                                                                            >  1:176414/1‑139 (MQ=255)

                                  ttCAAGCAAGGTGTCATATGTCGGCAGCGCGTTGAAGATTTCCGGTGTCGGGAAAGGAATATGC‑‑‑‑AGAAAGAAACCAATGCGATTATTCACTCCCTGTTTGCGTAATTCATGCGCAAATGGCAACAGGTGATAATCGTgg                                                                                       <  1:1112380/139‑1 (MQ=255)

                                  ttCAAGCAAGGTGTCATATGTCGGCAGCGCGTTGAAGATTTCCGGTGTCGGGAAAGGAATATGC‑‑‑‑AGAAAGAAACCAATGCGATTATTCACTCCCTGTTTGCGTAATTCATGCGCAAATGGCAACAGGTGATAATCGTgg                                                                                       >  1:1029532/1‑139 (MQ=255)

                                     aagcaagGTGTCATATGTCGGCAGCGCGTTGAAGATTTCCGGTGTCGGGAAAGGAATATGC‑‑‑‑AGAAAGAAACCAATGCGATTATTCACTCCCTGTTTGCGTAATTCATGCGCAAATGGCAACAGGTGATAATCGTGGATc                                                                                    <  2:758507/139‑1 (MQ=255)

                                       gcaagGTGTCATATGTCGGCAGCGCGTTGAAGATTTCCGGTGTCGGGAAAGGAATATGCAGAAAGAAAGAAACCAATGCGATTATTCACTCCCTGTTTGCGTAATTCATGCGCAAATGGCAACAGGTGATAATCGTGGa                                                                                      <  2:893233/139‑1 (MQ=255)

                                            gtgtCATATGTCGGCAGCGCGTTGAAGATTTCCGGTGTCGGGAAAGGAATATGC‑‑‑‑AGAAAGAAACCAATGCGATTATTCACTCCCTGTTTGCGTAATTCATGCGCAAATGGCaa                                                                                                       >  1:1130981/1‑113 (MQ=255)

                                            gtgtCATATGTCGGCAGCGCGTTGAAGATTTCCGGTGTCGGGAAAGGAATATGC‑‑‑‑AGAAAGAAACCAATGCGATTATTCACTCCCTGTTTGCGTAATTCATGCGCAAATGGCaa                                                                                                       <  2:1130981/113‑1 (MQ=255)

                                                  tatGTCGGCAGCGCGTTGAAGATTTCCGGTGTCGGGAAAGGAATATGC‑‑‑‑AGAAAGAAACCAATACGATTATTCACTCCCTGTTTGCGTAATTCATGCGCAAATGGCAACAGGTGATAATCGTGGATCCAGATAATGTCAt                                                                       <  2:172081/139‑1 (MQ=255)

                                                     gTCGGCAGCGCGTTGAAGATTTCCGGTGTCGGGAAAGGAATATGC‑‑‑‑AGAAAGAAACCAATGCGATTATTCACTCCCTGTTTGCGTAATTCATGCGCAAATGGCAACAGGTGa                                                                                                <  2:1145355/111‑1 (MQ=255)

                                                     gTCGGCAGCGCGTTGAAGATTTCCGGTGTCGGGAAAGGAATATGC‑‑‑‑AGAAAGAAACCAATGCGATTATTCACTCCCTGTTTGCGTAATTCATGCGCAAATGGCAACAGGTGa                                                                                                >  1:1145355/1‑111 (MQ=255)

                                                       cGGCAGCGCGTTGAAGATTTACGGTGTCGGGAAAGGAATATGC‑‑‑‑AGAAAGAAACCAATGCGATTATTCACTCCCTGTTTGCGAAATTCATGCGCAAATGGCAACAGGTGATAATCGTGGATCCAGATAATGTCATCGTCt                                                                  >  2:1115619/1‑139 (MQ=255)

                                                         gCAGCGCGTTGAAGATTTCCGGTGTCGGGAAAGGAATATGC‑‑‑‑AGAAAGAAACCAATGCGATTATTCACTCCCTGTTTGCGTAATTCATgcg                                                                                                                 <  1:276929/90‑1 (MQ=255)

                                                         gCAGCGCGTTGAAGATTTCCGGTGTCGGGAAAGGAATATGC‑‑‑‑AGAAAGAAACCAATGCGATTATTCACTCCCTGTTTGCGTAATTCATgcg                                                                                                                 >  2:276929/1‑90 (MQ=255)

                                                             cgcgTTGAAGATTTCCGGTGTCGGGAAAGGAATATGC‑‑‑‑AGAAAGAAACCAATGCGATTATTCACTCCCTGTTTGCGTAATTCATGCGCAAATGGCAACAGGTGATAATCGTGGATCCAGATAATGTCATCGTCTTGCAAc                                                            >  2:555640/1‑139 (MQ=255)

                                                               cgTTGAAGATTTCCGGTGTCGGGAAAGGAATATGC‑‑‑‑AGAAAGAAACCAATGCGATTATTCACTCCCTGTTTGCGTAATTCATGCGCAAATGGCAACAGGTGATAATCGTGGATCCAGATAATGTCATCGTCTTGCAACAg                                                          >  2:529476/1‑139 (MQ=255)

                                                                  tGAAGATTTCCGGTGTCGGGAAAGGAATATGC‑‑‑‑AGAAAGAAACCAATGCGATTATTCACTCCCTGTTTGCGTAATTCATGCGCAAATGGCAACAGGTGATAATCGTGGATCCAGATAATGTCATCGTCTTGCAACAGCgg                                                       <  1:529476/139‑1 (MQ=255)

                                                                  tGAAGATTTCCGGTGTCGGGAAAGGAATATGC‑‑‑‑AGAAAGAAACCAATGCGATTATTCACTCCCTGTTTGCGTAATTCATGCGCAAATGGCAACAGGTGATAATCGTGGATCCAGATAATGTCATCGTCTTGCAACAGCgg                                                       <  1:555640/139‑1 (MQ=255)

                                                                  tGAAGATTTCCGGTGTCGGGAAAGGAATATGC‑‑‑‑AGAAAGAAACCAATGCGATTATTCACTCCCTGTTTGCGTAATTCATGCGCAAATGGCAACAGGTGATAATCGTGGATCCAGATAATGTCATCGTCTTGCAACAGCgg                                                       <  2:1029532/139‑1 (MQ=255)

                                                                   gAAGATTTACGGTGTCGGGAAAGGAATATGC‑‑‑‑AGAAAGAAACCAATGCGATTATTCACTCCCTGTTTGCGAAATTCATGCGCAAATGGCAACAGGTGATAATCGTGGATCCAGATAATGTCATCGTCTTGCAACAGCGGc                                                      <  1:1115619/139‑1 (MQ=255)

                                                                          tCCGGTGTCGGGAAAGGAATATGC‑‑‑‑AGAAAGAAACCAATGCGATTATTCACTCCCTGTTTGCGTAATTCATGCGCAAATGGCAACAGGTGATAATCGTGGATCCAGATAATGTCATCGTCTTGCAACAGCGGCAGTAAt                                                >  1:173431/1‑138 (MQ=255)

                                                                          tCCGGTGTCGGGAAAGGAATATGC‑‑‑‑AGAAAGAAACCAATGCGATTATTCACTCCCTGTTTGCGTAATTCATGCGCAAATGGCAACAGGTGATAATCGTGGATCCAGATAATGTCATCGTCTTGCAACAGCGGCAGTAAt                                                <  2:173431/138‑1 (MQ=255)

                                                                            cGGTGTCGGGAAAGGAATATGC‑‑‑‑AGAAAGAAACCAATGCGATTATTCACTCCCTGTTTGCGTAATTCATGCGCAAATGGCAACAGGTGATAATCGTGGATCCAGATAATGTCATCGTCTTGCAACAGCGGCAGTAATTTa                                             <  2:862680/139‑1 (MQ=255)

                                                                                                         aagaaaCCAATGCGATTATTCACTCCCTGTTTGCGTAATTCATGCGCAAATGGCAACAGGTGATAATCGTGGATCCAGATAATGTCATCGTCTTGCAACAGCGGCAGTAATTTATCTGCCAGCAACGCATTTACGCGTa                    <  1:577395/139‑1 (MQ=255)

                                                                                                             aaCCAATGCGATTATTCACTCCCTGTTTGCGTAATTCATGCGCAAATGGCAACAGGTGATAATCGTGGATCCAGATAATGTCATCGTCTTGCAACAGCGGCAGTAATTTATCTGCCAGCAACGCATTTACGCGTAGATa                >  1:1189277/1‑139 (MQ=255)

                                                                                                                   tGCGATTATTCACTCCCTGTTTGCGTAATTCATGCGCaa                                                                                                              >  1:793938/1‑39 (MQ=255)

                                                                                                                   tGCGATTATTCACTCCCTGTTTGCGTAATTCATGCGCaa                                                                                                              <  2:793938/39‑1 (MQ=255)

                                                                                                                           ttCACTCCCTGTTTGCGTAATTCATGCGCAAATGGCAACAGGTGATAATCGTGGATCCAGATAATGTCATCGTCTTGCAACAGCGGCAGTAATTTATCTGCCACCAACGCATTTACGCGTAGATAGCCGTCCcaggcag  <  1:850257/139‑1 (MQ=255)

                                                                                                                                tCCCTGTTTGCGTAATTCATGCGCAAATGGCAACAGGTGATAATCGTGGATCCAGATAATGTCATCGTCTTGCAACAGCGGCAGTAATTTATCTGCCAGCAACGCAtt                            <  2:949817/108‑1 (MQ=255)

                                                                                                                                tCCCTGTTTGCGTAATTCATGCGCAAATGGCAACAGGTGATAATCGTGGATCCAGATAATGTCATCGTCTTGCAACAGCGGCAGTAATTTATCTGCCAGCAACGCAtt                            >  1:949817/1‑108 (MQ=255)

TCTGGAAACCCAGCAAATCATAATCACAAAGCTGTTCAAGCAAGGTGTCATATGTCGGCAGCGCGTTGAAGATTTCCGGTGTCGGGAAAGGAATATGC‑‑‑‑AGAAAGAAACCAATGCGATTATTCACTCCCCGTTTGCGTAATTCATGCGCAAATGGCAACAGGTGATAATCGTGGATCCAGATAATGTCATCGTCTTGCAACAGCGGCAGTAATTTATCTGCCAGCAACGCATTTACGCGTAGATAGCCGTCCCAGGCAG  >  NC_000913/1981054‑1981311

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`