Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,464,156	T→C	intergenic (+86/+96)	yfdF → / ← mlaA	uncharacterized protein/ABC transporter maintaining OM lipid asymmetry, OM lipoprotein component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,464,156	0	T	C	100.0%	152.9 / NA	42	intergenic (+86/+96)	yfdF/mlaA	uncharacterized protein/ABC transporter maintaining OM lipid asymmetry, OM lipoprotein component
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/18);  total (24/18)

AATGAAATTAATCAACCATCTGCGCCAGGTGTTAATTTTGATGATATTTAAAATTATTTTTCCAGTTTTTTGATTTTTCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCT  >  NC_000913/2464020‑2464283                                                                                                                                         |                                                                                                                                aaTGAAATTAATCAACCATCTGCGCCAGGTGTTAATTTTGATGATATTTAAAATTATTTTTCCAGTTTTTTGATTTTTCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGCCt                                                                                                                               <  1:510039/139‑1 (MQ=255)           aTCAACCATCTGCGCCAGGTGTTAATTTTGATGATATTTAAAATTATTTTTCCAGTTTTTTGATTTTTCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGCCTAGGTCATTGt                                                                                                                     >  1:1081318/1‑139 (MQ=255)           aTCAACCATCTGCGCCAGGTGTTAATTTTGATGATATTTAAAATTATTTTTCCAGTTTTTTGATTTTTCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGCCTAGGTCATTGt                                                                                                                     <  1:490576/139‑1 (MQ=255)                     tGCGCCAGGTGTTAATTTTGATGATATTTAAAATTATTTTTCCAGTTTTTTGATTTTTCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGCCTAGGTCATTGTCGGTTTAtct                                                                                                           >  2:1045144/1‑139 (MQ=255)                            ggTGTTAATTTTGATGATATTTAAAATTATTTTTCCAGTTTTTTGATTTTTCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGCCTAGGTCATTGTCGGTTTATCTCCTTTTa                                                                                                    >  2:34693/1‑139 (MQ=255)                              tgtTAATTTTGATGATATTTAAAATTATTTTTCCAGTTTTAGGATTTTTCAACAGCTGAATATCGGTAATAGGCAGTGACGAATGTCAATATTTGTACATAATTACGCCTAGGTCATTGGCGGTTGATCTCCTTTTACt                                                                                                  <  1:1195538/139‑1 (MQ=255)                               gtTAATTTTGATGATATTTAAAATTATTTTTCCAGTTTTTTGATTTTTCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGCCTAGGTCATTGTCGGTTTATCTCCTTTTACtt                                                                                                 >  2:476921/1‑139 (MQ=255)                                    ttttGATGATATTTAAAATTATTTTTCCAGTTTTTTGATTTTTCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGCCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGt                                                                                            <  1:476921/139‑1 (MQ=255)                                        gatgatATTTAAAATTATTTTTCCAGTTTTTTGATTTTTCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGCCTAGGTCATTGTCGGTTTAt                                                                                                             >  1:614090/1‑118 (MQ=255)                                        gatgatATTTAAAATTATTTTTCCAGTTTTTTGATTTTTCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGCCTAGGTCATTGTCGGTTTAt                                                                                                             <  2:614090/118‑1 (MQ=255)                                              atTTAAAATTATTTTTCCAGTTTTTTGATTTTTCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGCCTAGGTCATTGTCGGTTTAtct                                                                                                           >  1:460665/1‑114 (MQ=255)                                              atTTAAAATTATTTTTCCAGTTTTTTGATTTTTCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGCCTAGGTCATTGTCGGTTTAtct                                                                                                           <  2:460665/114‑1 (MQ=255)                                              atTTAAAATTATTTTTCCAGTTTTTTGATTTTTCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGCCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGa                                                                                  >  2:952249/1‑139 (MQ=255)                                               tttAAAATTATTTTTCCAGTTTTTTGATTTTTCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGCCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGAt                                                                                 <  1:34693/139‑1 (MQ=255)                                                        attTTTCCAGTTTTTTGATTTTTCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGCCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACaaa                                                                        >  1:951002/1‑139 (MQ=255)                                                        attTTTCCAGTTTTTTGATTTTTCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGCCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACaaa                                                                        >  1:999674/1‑139 (MQ=255)                                                                   tttttGATTTTTCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGCCTAGGTCATTGTCGGTTTATCTCCTTTTACt                                                                                                  >  1:430101/1‑102 (MQ=255)                                                                   tttttGATTTTTCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGCCTAGGTCATTGTCGGTTTATCTCCTTTTACt                                                                                                  <  2:430101/102‑1 (MQ=255)                                                                            tttCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGCCTAGGTCATTGTCGGTTTAt                                                                                                             >  1:1199221/1‑82 (MQ=255)                                                                            tttCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGCCTAGGTCATTGTCGGTTTAt                                                                                                             <  2:1199221/82‑1 (MQ=255)                                                                                     cTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGCCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCtttttt                                           >  1:877466/1‑139 (MQ=255)                                                                                     cTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGCCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCtttttt                                           >  2:1053082/1‑139 (MQ=255)                                                                                         atatCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGCCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTAttt                                       <  2:877466/139‑1 (MQ=255)                                                                                                     gTCAGTGACGAACATCAGTATTTTTACATAATTACGCCTAGTTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCa                           >  2:197750/1‑139 (MQ=255)                                                                                                       cAGTGACGAACATCAGTATTTTTACATAATTACGCCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGa                         <  2:951002/139‑1 (MQ=255)                                                                                                          tGACGAACATCAGTATTTTTACATAATTACGCCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATc                      >  1:29813/1‑139 (MQ=255)                                                                                                          tGACGAACATCAGTATTTTTACATAATTACGCCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATc                      >  2:934503/1‑139 (MQ=255)                                                                                                              gAACATCAGTATTTTTACATAATTACGCCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAata                  <  1:1053082/139‑1 (MQ=255)                                                                                                               aaCATCAGTATTTTTACATAATTACGCCTAGGTCATTGTCGGTTTa                                                                                                              <  1:752875/46‑1 (MQ=255)                                                                                                               aaCATCAGTATTTTTACATAATTACGCCTAGGTCATTGTCGGTTTa                                                                                                              >  2:752875/1‑46 (MQ=255)                                                                                                               aaCATCAGTATTTTTACATAATTACGCCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAatat                 >  1:39257/1‑139 (MQ=255)                                                                                                                    cAGTATTTTTACATAATTACGCCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCtttt                                             >  1:82453/1‑106 (MQ=255)                                                                                                                    cAGTATTTTTACATAATTACGCCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCtttt                                             <  2:82453/106‑1 (MQ=255)                                                                                                                    cAGTATTTTTACATAATTACGCCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCtttt            >  2:232417/1‑139 (MQ=255)                                                                                                                       tATTTTTACATAATTACGCCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTaaa         <  2:39257/139‑1 (MQ=255)                                                                                                                              aCATAATTACGCCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACt                                                     >  2:461852/1‑88 (MQ=255)                                                                                                                              aCATAATTACGCCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACt                                                     <  1:461852/88‑1 (MQ=255)                                                                                                                              aCATAATTACGCCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCt  >  2:864361/1‑139 (MQ=255)                                                                                                                               cATAATTACGCCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGTGACt                                                     >  2:1022884/1‑87 (MQ=255)                                                                                                                               cATAATTACGCCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGTGACt                                                     <  1:1022884/87‑1 (MQ=255)                                                                                                                                 tAATTACGCCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCa                                                   <  2:650422/87‑1 (MQ=255)                                                                                                                                 tAATTACGCCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCa                                                   >  1:650422/1‑87 (MQ=255)                                                                                                                                         |                                                                                                                                AATGAAATTAATCAACCATCTGCGCCAGGTGTTAATTTTGATGATATTTAAAATTATTTTTCCAGTTTTTTGATTTTTCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCT  >  NC_000913/2464020‑2464283

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑