Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,204,189	G→A	intergenic (‑58/+32)	bacA ← / ← folB	undecaprenyl pyrophosphate phosphatase/dihydroneopterin aldolase and dihydroneopterin triphosphate 2'‑epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,204,189	0	G	A	100.0%	158.0 / NA	47	intergenic (‑58/+32)	bacA/folB	undecaprenyl pyrophosphate phosphatase/dihydroneopterin aldolase and dihydroneopterin triphosphate 2'‑epimerase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (30/17);  total (30/17)

ATACCGGCAGAAATTCTGTCAATCCTTCGACCACACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATT‑AAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAGGTTATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGATTATTGCCACGCTCAATGATTACGCCAACATTCGCCGCCCGCGCCACTGCGCCTGGCTTGCTGAGTTTGATACGCACCCACGG  >  NC_000913/3204056‑3204322                                                                                                                                       |                                                                                                                                      aTACCGGCAGAAATTCTGTCAATCCTTCGACCACACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATT‑AAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAAGTtat                                                                                                                                  >  2:525399/1‑139 (MQ=255)       gCAGAAATTCTGTCAATCCTTCGACCACACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATT‑AAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAAGTTATAACCGt                                                                                                                            >  2:1049691/1‑139 (MQ=255)       gCAGAAATTCTGTCAATCCTTCGACCACACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATT‑AAAATGTACCGCTTGTCCGAACTACTGCGTATAACCAAGTTATAACCGt                                                                                                                            >  1:830176/1‑139 (MQ=255)       gCAGAAATTCTGTCAATCCTTCGACCACACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATT‑AAAATGTACCGCTTGTCCGAACTACTGAGTATGACCAAGTTATAACCGt                                                                                                                            >  1:711519/1‑139 (MQ=255)        cAGAAATTCTGTCAATCCTTCGACCACACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATT‑AAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAAGTTATAACCGtt                                                                                                                           <  2:133511/139‑1 (MQ=255)              ttCTGTCAATCCTTCGACCACACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATT‑AAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAAGTTATAACCGTTTGGttt                                                                                                                     <  2:128503/139‑1 (MQ=255)                        ccTTCGACCACACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATT‑AAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAAGTTATAACCGTTTGGTTTAACAGCTGTa                                                                                                           <  2:179531/139‑1 (MQ=255)                                   acCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATT‑AAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAAGTTATAACCGTTTGGTTTAACAGCTGTAAAATTAattat                                                                                                >  2:639772/1‑139 (MQ=255)                                             gCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAAGTTATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCa                                                                                       <  2:392820/139‑1 (MQ=255)                                             gCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATTAAAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAAGTTATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCa                                                                                       >  1:392820/1‑139 (MQ=255)                                             gCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATT‑AAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAAGTTATAACCGTTTGGTTTAAc                                                                                                                  >  2:81559/1‑111 (MQ=255)                                             gCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATT‑AAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAAGTTATAACCGTTTGGTTTAAc                                                                                                                  <  1:81559/111‑1 (MQ=255)                                             gCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATT‑AAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAAGTTATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAg                                                                                      >  2:754428/1‑139 (MQ=255)                                              ccGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATT‑AAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAAGTTATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGa                                                                                     >  1:1029927/1‑139 (MQ=255)                                                 cTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATT‑AAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAAGTTATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGatta                                                                                  >  2:727618/1‑139 (MQ=255)                                                 cTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATT‑AAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAAGTTATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGatta                                                                                  >  1:1033029/1‑139 (MQ=255)                                                       gcagcGAGTGCATATCGCTCATCAATAAACCCCTAAATTATT‑AAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAAGTTATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGATTATTGCCa                                                                            <  1:238007/139‑1 (MQ=255)                                                            gAGTGCATATCGCTCATCAATAAACCCCTAAATTATT‑AAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAAGTTATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGATTATTGCCa                                                                            >  2:1127783/1‑134 (MQ=255)                                                            gAGTGCATATCGCTCATCAATAAACCCCTAAATTATT‑AAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAAGTTATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGATTATTGCCa                                                                            <  1:1127783/134‑1 (MQ=255)                                                            gAGTGCATATCGCTCATCAATAAACCCCTAAATTATT‑AAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAAGTTATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGATTATTGCCACGCTc                                                                       <  1:727618/139‑1 (MQ=255)                                                                  atatCGCTCATCAATAAACCCCTAACTTATT‑AACATGTACCGCTTGGCCGAACTACTGCGTATGACCAAGTTATAAGCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGATTATTGCCATGCTCAATGAt                                                                 >  2:1080883/1‑139 (MQ=255)                                                                  atatCGCTCATCAATAAACCCCTAAATTATT‑AAAATGTACCGCTTGTCCGAACTACTGCGTATAACCAAGTTATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGATTATTGCCACGCTCAATGAt                                                                 <  2:830176/139‑1 (MQ=255)                                                                        cTCATCAATAAACCCCTAAATTATT‑AAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAAGTTATAACCGTTTGGTTTAAc                                                                                                                  <  2:629083/84‑1 (MQ=255)                                                                        cTCATCAATAAACCCCTAAATTATT‑AAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAAGTTATAACCGTTTGGTTTAAc                                                                                                                  >  1:629083/1‑84 (MQ=255)                                                                          catcaATAAACCCCTAAATTATT‑AAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAAGTTATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGATTATTGcc                                                                             <  2:1020029/119‑1 (MQ=255)                                                                          catcaATAAACCCCTAAATTATT‑AAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAAGTTATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGATTATTGcc                                                                             >  1:1020029/1‑119 (MQ=255)                                                                           atcaATAAACCCCTAAATTATT‑AAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAAGTTATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGATTATTGCCACGCTCAATGATTACGCCAAc                                                        >  1:357134/1‑139 (MQ=255)                                                                                tAAACCCCTAAATTATT‑AAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAAGTTATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGATTATTGCCACGCTCAATGATTACGCCAACATTcg                                                   >  2:135751/1‑139 (MQ=255)                                                                                     cccTAAATTATT‑AAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAAGTTATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGat                                                                                    <  2:151493/101‑1 (MQ=255)                                                                                     cccTAAATTATT‑AAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAAGTTATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGat                                                                                    >  1:151493/1‑101 (MQ=255)                                                                                        tAAATTATT‑AAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAAGTTATAACCGTTTGGTTTAACAg                                                                                                                <  1:356907/70‑1 (MQ=255)                                                                                        tAAATTATT‑AAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAAGTTATAACCGTTTGGTTTAACAg                                                                                                                >  2:356907/1‑70 (MQ=255)                                                                                         aaaTTATT‑AAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAAGTTATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGATTATTGCCACGCTCAATGATTACGCCAACATTCGCCGCCcgcg                                          <  2:1033029/139‑1 (MQ=255)                                                                                          aaTTATT‑AAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAAGTTATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGATTATTGCCACGCTCAATGATTACGCCAACATTCGCCGCCCgcgc                                         >  1:159915/1‑139 (MQ=255)                                                                                          aaTTATT‑AAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAAGTTATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGATTATTGCCACGCTCAATGATTACGCCAACATTCGCCGCCCgcgc                                         >  2:743853/1‑139 (MQ=255)                                                                                                     aTGTACCGCTTGTCCGAACTACTGCGTATGACCAAGTTATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGATTATTGCCACGCTCAATGATTACGCCAACATTCGCCGCCCGCGCCACTGCGCCt                               <  2:159915/139‑1 (MQ=255)                                                                                                        tACCGCTTGTCCGAACTACTGCGTATGACCAAGTTATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGATTATTGCCACGCTCAATGATTACGCCAACATTCGCCGCCCGCGCCACTGCGCCTGGc                            >  2:962353/1‑139 (MQ=255)                                                                                                          ccGCTTGTCCGAACTACTGCGTATGACCAAGTTATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGATTATTGCCACGCTCAATGATTACGCCAACATTCGCCGCCCGCGCCACTGCGCCTGGCtt                          >  2:621202/1‑139 (MQ=255)                                                                                                          ccGCTTGTCCGAACTACTGCGTATGACCAAGTTATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGATTATTGCCACGCTCAATGATTACGCCAACATTCGCCGCCCGCGCCACTGCGCCTGGCtt                          >  2:1007358/1‑139 (MQ=255)                                                                                                          ccGCTTGTCCGAACTACTGCGTATGACCAAGTTATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGATTATTGCCACGCTCAATGATTACGCCAACATTCGCCGCCCGCACCACTGCGCCTGGCtt                          >  1:854453/1‑139 (MQ=255)                                                                                                          ccGCTTGTCCGAACTACTGAGTATGACCAAGTTATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGATTATTGCCACGCTCAATGATTACGCCAACATTCGCCGCCCGCGCCACTGCGCCTGGCtt                          >  2:449520/1‑139 (MQ=255)                                                                                                                  ccGAACTACTGCGTCTGACCAAGTTATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGAGTATTGCCACGCTCAATGATTACGCCAGCATTCGCCGCCCGCGCCACTGCGCCTGGCTTGCTGAGtt                  >  1:733904/1‑139 (MQ=255)                                                                                                                     aaCTACTGCGTATGACCAAGTTATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGATTATTGCCACGCTCAATGATTACGCCAACATTCGCCGCCCGCGCCACTGCGCCTGGCTTGCTGAGTTTGa               >  1:1091264/1‑139 (MQ=255)                                                                                                                         actGCGTATGACCAAGTTATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGATTATTGCCACGCTCAATGATTACGCCAACATTCGCCGCCCGCGCCACTGCGCCTGGCTTGCTGAGTTTGATACg           <  1:754428/139‑1 (MQ=255)                                                                                                                                aTGACCAAGTTATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGATTATTGCCACGCTCAATGATTACGCCAACATTCGCCGCCCGCGCCACTGCGCCTGGCTTGCTGAGTTTGATACGCACCCAc    >  1:741241/1‑139 (MQ=255)                                                                                                                                 tGACCAAGTTATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGATTATTGCCACGCTCAATGATTACGCCAACATTCGCCGCCCGCACCACTGCGCCTGGCTTGCTGAGTTTGATACGCACCCACg   <  2:854453/139‑1 (MQ=255)                                                                                                                                  gACCAAGTTATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGATTATTGCCACGCTCAATGATTACGCCAACATTCGCCGCCCGCGCCACTGCGCCTGGCTTGCTGAGTTTGATACGCACCCACgg  >  2:353393/1‑139 (MQ=255)                                                                                                                                       |                                                                                                                                      ATACCGGCAGAAATTCTGTCAATCCTTCGACCACACCCAATATTGCCGCTATCAGCAGCGAGTGCATATCGCTCATCAATAAACCCCTAAATTATT‑AAAATGTACCGCTTGTCCGAACTACTGCGTATGACCAGGTTATAACCGTTTGGTTTAACAGCTGTAAAATTAATTATTTTCTTTCAGATTATTGCCACGCTCAATGATTACGCCAACATTCGCCGCCCGCGCCACTGCGCCTGGCTTGCTGAGTTTGATACGCACCCACGG  >  NC_000913/3204056‑3204322

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑