Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	323,702	T→C	A455A (GCT→GCC)	ykgF →	ferridoxin‑like LutB family protein, putative electron transport chain YkgEFG component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	323,702	0	T	C	100.0%	186.2 / NA	50	A455A (GCT→GCC)	ykgF	ferridoxin‑like LutB family protein, putative electron transport chain YkgEFG component
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/20);  total (30/20)

ATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCGACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGTTGCTCAGGCACTGGGTCGCCCGCTGCGACTTGAACCGCAAGC  >  NC_000913/323566‑323831                                                                                                                                         |                                                                                                                                  aTAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCGACTGGATGGAAGCACGCGATCTTCCTGAAGCCGa                                                                                                                                 >  1:262869/1‑139 (MQ=255)     tCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCGACTGGATGGAAGCACGCGATCTTCCTGAAGCCGACGga                                                                                                                             <  2:1016797/139‑1 (MQ=255)     tCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCGACTGGATGGAAGCACGCGATCTTCCTGAAGCCGACGga                                                                                                                             >  2:425053/1‑139 (MQ=255)      cATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCGACTGGATGGAAGCACGCGATCTTCCTGAAGCCGACGgag                                                                                                                            >  2:510365/1‑139 (MQ=255)      cATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCGACTGGATGGAAGCACGCGATCTTCCTGAAGCCGACGgag                                                                                                                            <  1:230672/139‑1 (MQ=255)       aTCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCGACTGGATGGAAGCACGCGATCTTCCTGAAGCCGACGgaga                                                                                                                           >  2:203643/1‑139 (MQ=255)         ccAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCGACTGGATGGAAGCACGCGATCTTCCTGAAGCCGACGgagaga                                                                                                                         >  2:1110450/1‑139 (MQ=255)          cAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCGACTGGATGGAAGCACGCGATCTTCCTGAAGCCGACGGagagag                                                                                                                        <  1:510365/139‑1 (MQ=255)            ggATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCGACTGGATGGAAGCACGCGATCTTCCTGAAGCCGACGGAGAGAGtt                                                                                                                      >  1:506604/1‑139 (MQ=255)                       aGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCGACTGGATGGAAGCACGCGATCTTCCTGAAGCCGACGGAGAGAGTTTCCGTAGTTgg                                                                                                           >  2:796129/1‑139 (MQ=255)                       aGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCGACTGGATGGAAGCACGCGATCTTCCTGAAGCCGACGGAGAGAGTTTCCGTAGTTgg                                                                                                           >  1:868663/1‑139 (MQ=255)                        gTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCGACTGGATGGAAGCACGCGATCTTCCTGAAGCCGACGGAGAGAGTTTCCGTAGTTGGt                                                                                                          <  1:846155/139‑1 (MQ=255)                            ggATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCGACTGGATGGAAGCACGCGATCTTCCTGAAGCCGACGGAGAGAGTTTCCGTAGTTGGTTTaa                                                                                                      >  2:673065/1‑139 (MQ=255)                            ggATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCGACTGGATGGAAGCACGCGATCTTCCTGAAGCCGACGGAGAGAGTTTCCGTAGTTGGTGTaa                                                                                                      >  2:257852/1‑139 (MQ=255)                                    gCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCGACTGGATGGAAGCACGCGATCTTCCTGAAGCCGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATc                                                                                              <  2:262869/139‑1 (MQ=255)                                         tGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCGACTGGATGGAAGCACGCGATCTTCCTGAAGCCGACGGAGAGAGTTTCCGTAGt                                                                                                              <  1:399320/118‑1 (MQ=255)                                         tGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCGACTGGATGGAAGCACGCGATCTTCCTGAAGCCGACGGAGAGAGTTTCCGTAGt                                                                                                              >  2:399320/1‑118 (MQ=255)                                            tCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCGACTGGATGGAAGCACGCGATCTTCCTGAAGCCGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAg                                                                                      <  1:203643/139‑1 (MQ=255)                                              aTGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCGACTGGATGGAAGCACGCGATCTTCCTGAAGCCGACGGAGAGAGTTTCCGTAGt                                                                                                              <  2:118812/113‑1 (MQ=255)                                              aTGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCGACTGGATGGAAGCACGCGATCTTCCTGAAGCCGACGGAGAGAGTTTCCGTAGt                                                                                                              >  1:118812/1‑113 (MQ=255)                                              aTGCGACAAGCGGGTATATCAATGGCTGCAATACACCACGCAAATTTGGCCCGATTAGCGTCGGGGTGGAAGCACGCGATCTTCCTGAAGCAGATGGAGCGGGTTTCTGTTGTGGGTTTAAGTAACATGAGGCGTAggc                                                                                    >  2:411477/1‑138 (MQ=255)                                                   ggaaGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCGACTGGATGGAAGCACGCGATCTTCCTGAAGCCGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGaaaa                                                                               >  2:199914/3‑139 (MQ=255)                                                        cTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCGACTGGATGGAAGCACGCGATCTTCCTGAAGCCGACGGAGAGAGTTTCCGTAg                                                                                                               >  1:122728/1‑102 (MQ=255)                                                        cTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCGACTGGATGGAAGCACGCGATCTTCCTGAAGCCGACGGAGAGAGTTTCCGTAg                                                                                                               <  2:122728/102‑1 (MQ=255)                                                           gTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCGACTGGATGGAAGCACGCGATCTTCCTGAAGCCGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGa                                                                       >  1:485511/1‑139 (MQ=255)                                                            tttATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGGGACTGGATGGAAGCACGCGATCTTCCTGAAGCCGACGGAGAGAGTTTc                                                                                                                    >  2:854198/1‑93 (MQ=255)                                                            tttATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCGACTGGATGGAAGCACGCGATCTTCCTGAAGCCGACGGAGAGAGTTTc                                                                                                                    <  1:854198/93‑1 (MQ=255)                                                                     ggcggcAAAACACCACTCAAATTTGGCGCGATTAGCGACTGGATGGAAGCACGCGATCTTCCTGAAGCCGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGc                                                             >  2:540539/1‑139 (MQ=255)                                                                      gcggcAAAACACCACTCAAATTTGGCGCGATTAGCGACTGGATGGAAGCACGCGATCTTCCTGAAGCCGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCg                                                            >  2:455677/1‑139 (MQ=255)                                                                       cggcAAAACACCACTCAAATTTGGCGCGATTAGCGACTGGATGGAAGCACGCGATCTTCCTGAAGCCGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGaaaaa                                                                              <  2:955574/120‑1 (MQ=255)                                                                       cggcAAAACACCACTCAAATTTGGCGCGATTAGCGACTGGATGGAAGCACGCGATCTTCCTGAAGCCGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGaaaaa                                                                              >  1:955574/1‑120 (MQ=255)                                                                                      cAAATTTGGCGCGATTAGCGACTGGATGGAAGCACGCGATCTTCCTGAAGCCGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGt                                            <  1:796129/139‑1 (MQ=255)                                                                                      cAAATTTGGCGCGATTAGCGACTGGATGGAAGCACGCGATCTTCCTGAAGCCGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGt                                            <  2:642814/139‑1 (MQ=255)                                                                                       aaaTTTGGCGCGATTAGCGACTGGATGGAAGCACGCGATCTTCCTGAAGCCGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGtt                                           >  2:808209/1‑139 (MQ=255)                                                                                          tttGGCGCGATTAGCGACTGGATGGAAGCACGCGATCTTCCTGAAGCCGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGTTGCt                                        <  1:871911/139‑1 (MQ=255)                                                                                              gcgcGATTAGCGACTGGATGGAAGCACGCGATCTTCCTGAAGCCGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGTTGCTCAgg                                    >  2:312147/1‑139 (MQ=255)                                                                                               cgcgATTAGCGACTGGATGGAAGCACGCGATCTTCCTGAAGCCGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGTTGATCAGGc                                   >  2:258113/1‑139 (MQ=255)                                                                                                              gatggaAGCACGCGATCTTCCTGAAGCCGACGGAGAGAGTTTCCGTAg                                                                                                               >  1:918224/1‑48 (MQ=255)                                                                                                              gatggaAGCACGCGATCTTCCTGAAGCCGACGGAGAGAGTTTCCGTAg                                                                                                               <  2:918224/48‑1 (MQ=255)                                                                                                              gatggaAGCACGCGATCTTCCTGAAGCCGACGGAGAGAGTTTCCGTAGt                                                                                                              >  1:499142/1‑49 (MQ=255)                                                                                                              gatggaAGCACGCGATCTTCCTGAAGCCGACGGAGAGAGTTTCCGTAGt                                                                                                              <  2:499142/49‑1 (MQ=255)                                                                                                              gatggaAGCACGCGATCTTCCTGAAGCCGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGTTGCTCAGGCACTGGGTCGCCCGCt                    >  1:255881/1‑139 (MQ=255)                                                                                                                   aaGCACGCGATCTTCCTGAAGCCGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGTTGCTCAGGCACTGGGTCGCCCGCTGCGAc               >  2:522122/1‑139 (MQ=255)                                                                                                                   aaGCACGCGATCTTCCTGAAGCCGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGTTGCTCAGGCACTGGGTCGCCCGCTGCGAc               >  2:1104896/1‑139 (MQ=255)                                                                                                                     gCACGCGATCTTCCTGAAGCCGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGTTGCTCAGGCACTGGGTCGCCCGCTGCGACtt             <  2:48105/139‑1 (MQ=255)                                                                                                                      cACGCGATCTTCCTGAAGCCGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGTTGCTCAGGCACTGGGTCGCCCGCTGCGACTTg            >  1:379749/1‑139 (MQ=255)                                                                                                                        cgcgATCTTCCTGAAGCCGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAAttt                                                       <  2:111116/94‑1 (MQ=255)                                                                                                                        cgcgATCTTCCTGAAGCCGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAAttt                                                       >  1:111116/1‑94 (MQ=255)                                                                                                                        cgcgATCTTCCTGAAGCCGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGTTGCt                                        >  1:218484/1‑109 (MQ=255)                                                                                                                        cgcgATCTTCCTGAAGCCGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGTTGCt                                        <  2:218484/109‑1 (MQ=255)                                                                                                                                tCCTGAAGCCGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGTTGCTCAGGCACTGGGTCGCCCGCTGCGACTTGAACCGCAAGc  <  2:506604/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                                  ATAGTCATCCAGGATTGTGGAAAGTCGGGATGATGGCCGGTGCTCATGCGGCAAGCTGGTTTATCAATGGCGGCAAAACACCACTCAAATTTGGCGCGATTAGCGACTGGATGGAAGCACGCGATCTTCCTGAAGCTGACGGAGAGAGTTTCCGTAGTTGGTTTAAGAAACATCAGGCGCAGGAGAAAAAGAATGGATAATCGAGGCGAATTTTTGAATAACGTTGCTCAGGCACTGGGTCGCCCGCTGCGACTTGAACCGCAAGC  >  NC_000913/323566‑323831

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑