Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 3,464,087 | A→T | I56F (ATC→TTC) | gspL → | general secretory pathway component, cryptic |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,464,087 | 0 | A | T | 100.0% | 221.9 / NA | 63 | I56F (ATC→TTC) | gspL | general secretory pathway component, cryptic |
Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (27/36); total (27/36) |
CTCGACGCTGCGTAAGCATTGGGAATGGATGACGTTCTCCGCAGATAGCGTATCTTCGGTACATACGCTCACGGACGATCTTCCGCTGGAGTCGCTGGCCGATCAGCCCGGTGCCGGAAATGTTCATCTTTTGATCCCACCAGAGGGCCTTTTATATCGTTCCCTCACGCTGCCCAACGCCAAATACAAACTCACCGCACAAACATTACAATGGCTGGCAGAGGAGACGTTACCCGACAACACCCAGGACTGGCACTGGACGGTT > NC_000913/3463954‑3464218 | cTCGACGCTGCGTAAGCATTGGGAATGGATGACGTTCTCCGCAGATAGCGTATCTTCGGTACATACGCTCACGGACGATCTTCCGCTGGAGTCGCTGGCCGATCAGCCCGGTGCCGGAAATGTTCATCTTTTGTTCcca > 2:326172/1‑139 (MQ=255) cTGCGTAAGCATTGGGAATGGATGACGTTCTCCGCAGATAGCGTATCTTCGGTACATACGCTCACGGACGATCTTCCGCTGGAGTCGCTGGCCGATCAGCCCGGTGCCGGAAATGTTCATCTTTTGTTCCCACCAGAgg < 1:483790/139‑1 (MQ=255) cTGCGTAAGCATTGGGAATGGATGACGTTCTCCGCAGATAGCGTATCTTCGGTACATACGCTCACGGACGATCTTCCGCTGGAGTCGCTGGCCGATCAGCCCGGTGCCGGAAATGTTCATCTTTTGTTCCCACCAGAgg > 2:705825/1‑139 (MQ=255) cTGCGTAAGCATTGGGAATGGATGACGTTCTCCGCAGATAGCGTATCTTCGGTACATACGCTCACGGACGATCTTCCGCTGGAGTCGCTGGCCGATCAGCCCGGTGCCGGAAATGTTCATCTTTTGTTCCCACCAGAgg < 1:87428/139‑1 (MQ=255) tGCGTAAGCATTGGGAATGGATGACGTTCTCCGCAGATAGCGTATCTTCGGTACATACGCTCACGGACGATCTTCCGCTGGAGTCGCTGGCCGATCAGCCCGGTGCCGGAAATGTTCATCTTTTGTTCCCACCAGAggg < 1:705825/139‑1 (MQ=255) cATTGGGAATGGATGACGTTCTCCGCAGATAGCGTATCTTCGGTACATACGCTCACGGACGATCTTCCGCTGGAGTCGCTGGCCGATCAGCCCGGTGCCGGAAATGTTCATCTTTTGTTCCCACCAGAGGGCCTTTtat < 1:539023/139‑1 (MQ=255) gggAATGGATGACGTTCTCCGCAGATAGCGTATCTTCGGTACATACGCTCACGGACGATCTTCCGCTGGAGTCGCTGGCCGATCAGCCCGGTGCCGGAAATGTTCATCTTTTGTTCCCACCAGAGGGCCTTTTATATCg > 2:478443/1‑139 (MQ=255) gggAATGGATGACGTTCTCCGCAGATAGCGTATCTTCGGTACATACGCTCACGGACGATCTTCCGCTGGAGTCGCTGGCCGATCAGCCCGGTGCCGGAAATGTTCATCTTTTGTTCCCACCAGAGGGCCTTTTATATCg < 2:1110695/139‑1 (MQ=255) gATGACGTTCTCCGCAGATAGCGTATCTTCGGTACATACGCTCACGGACGATCTTCCGCTGGAGTCGCTGGCCGATCAGCCCGGTGCCGGAAATGTTCATCTTTTGTTCCCACCAGAGGGCCTTTTATATCGTTCCCTc > 2:333853/1‑139 (MQ=255) gACGTTCTCCGCAGATAGCGTATCTTCGGTACATACGCTCACGGACGATCTTCCGCTGGAGTCGCTGGCCGATCAGCCCGGTGCCGGAAATGTTCATCTTTTGTTCCCACCAGAGGGCCTTTTATATCGTTCCCTCACg < 1:378518/139‑1 (MQ=255) aCGTTCTCCGCAGATAGCGTATCTTCGGTACATACGCTCACGGACGATCTTCCGCTGGAGTCGCTGGCCGATCAGCCCGGTGCCGGAAATGTTCATCTTTTGTTCCCACCAGAGGGCCTTTTATATCGTTCCCTCACGc > 1:688581/1‑139 (MQ=255) cGTTCTCCGCAGATAGCGTATCTTCGGTACATACGCTCACGGACGATCTTCCGCTGGAGTCGCTGGCCGATCAGCCCGGTGCCGGAAATGTTCATCTTATGTTCCCACCAGAGGGCCtt > 2:341/1‑119 (MQ=255) cGTTCTCCGCAGATAGCGTATCTTCGGTACATACGCTCACGGACGATCTTCCGCTGGAGTCGCTGGCCGATCAGCCCGGTGCCGGAAATGTTCATCTTATGTTCCCACCAGAGGGCCtt < 1:341/119‑1 (MQ=255) tctcCGCAGATAGCGTATCTTCGGTACATACGCTCACGGACGATCTTCCGCTGGAGTCGCTGGCCGATCAGCCCGGTGCCGGAAATGTTCATCTTTTGTTCCCACCAGAGGGCCTTTTATATCGTTCCCTCACGCTGcc < 1:471539/139‑1 (MQ=255) tcCGCAGATAGCGTATCTTCGGTACATACGCTCACGGACGATCTTCCGCTGGAGTCGCTGGCCGATCAGCCCGGTGCCGGCAATGTTCATCTTTTGTTCCCACCAGAGGGCCTTTTATATCGTTCCCTCACGCTGCCCa > 1:374514/1‑139 (MQ=255) tcCGCAGATAGCGTATCTTCGGTACATACGCTCACGGACGATCTTCCGCTGGAGTCGCTGGCCGATCAGCCCGGTGCCGGAAATGTTCATCTTTTGTTCCCACCAGAGGGCCTTTTATATCGTTCCCTCACGCTGCCCa < 2:376401/139‑1 (MQ=255) tcCGCAGATAGCGTATCTTCGGTACATACGCTCACGGACGATCTTCCGCTGGAGTCGCTGGCCGATCAGCCCGGTGCCGGAAATGTTCATCTTTTGTTCCCACCAGAGGGCCTTTTATATCGTTCCCTCACGCTGCCCa < 1:478443/139‑1 (MQ=255) cAGATAGCGTATCTTCGGTACATACGCTCACGGACGATCTTCCGCTGGAGTCGCTGGCCGATCAGCCCGGTGCCGGAAATGTTCATCTTTTGTTCCCACCAGAGGGCCTTTTATATCGTTCCCTCACGCTGCCCAACGc < 2:688581/139‑1 (MQ=255) gATAGCGTATCTTCGGTACATACGCTCACGGACGATCTTCCGCTGGAGTCGCTGGCCGATCAGCCCGGTGCCGGAAATGTTCATCTTTTGTTCCCACCAGAGGGCCTTTTATATCGTTCCCTCACGCTGCCCAACGCCa < 1:742346/139‑1 (MQ=255) gATAGCGTATCTTCGGTACATACGCTCACGGACGATCTTCCGCTGGAGTCGCTGGCCGATCAGCCCGGTGCCGGAAATGTTCATCTTTTGTTCCCACCAGAGGGCCTTTTATATCGTTCCCTCACGCTGCCCAACGCCa < 2:935594/139‑1 (MQ=255) gATAGCGTATCTTCGGTACATACGCTCACGGACGATCTTCCGCTGGAGTCGCTGGCCGATCAGCCCGGTGCCGGAAATGTTCATCTTTTGTTCCCACCAGAGGGCCTTTTATATCGTTCCCTCACGCTGCCCAACGCCa > 2:970911/1‑139 (MQ=255) gATAGCGTATCTTCGGTACATACGCTCACGGACGATCTTCCGCTGGAGTCGCTGGCCGATCAGCCCGGTGCCGGAAATGTTCATCTTTTGTTCCCACCAGAGGGCCTTTTATATCGTTCCCTCACGCTGCCCAACGCCa < 1:7797/139‑1 (MQ=255) gATAGCGTATCTTCGGTACATACGCTCACGGACGATCTTCCGCTGGAGTCGCTGGCCGATCAGCCCGGTGCCGGAAATGTTCATCTTTTGTTCCCACCAGAGGGCCTTTTATATCGTTCCCTCACGCTGCCCAACGCCa < 1:147338/139‑1 (MQ=255) ttCGGTACATACGCTCAGGGACGATCTTCCGCTGGAGTCGCTGGCCGATCAGCCCGGTGCCGGAAATGTTCATCTTTTGTTCCCACCAGAGGGCCTTTTATATCGTTCCCTCACGCTGCCCAACGCCAAATACAAACTc > 1:755974/1‑139 (MQ=255) tCGGTACATACGCTCACGGACGATCTTCCGCTGGAGTCGCTGGCCGATCAGCCCGGTGCCGGAAATGTTCATCTTTTGTTCCCACCAGAGGGCCTTTTATATCGTTCCCTCACGCTGCCCAACGCCAAATACAAACTcc < 2:755974/139‑2 (MQ=255) tCGGTACATACGCTCACGGACGATCTTCCGCTGGAGTCGCTGGCCGATCAGCCCGGTGCCGGAAATGTTCATCTTTTGTTCCCACCAGAGGGCCTTTTATATCGTTCCCTCACGCTGCCCAACGCCAAATACAAACTCa < 1:136148/139‑1 (MQ=255) tCGGTACATACGCTCACGGACGATCTTCCGCTGGAGTCGCTGGCCGATCAGCCCGGTGCCGGAAATGTTCATCTTTTGTTCCCACCAGAGGGCCTTTTATATCGTTCCCTCACGCTGCCCAACGCCAAATACAAACTCa > 1:1117909/1‑139 (MQ=255) tCGGTACATACGCTCACGGACGATCTTCCGCTGGAGTCGCTGGCCGATCAGCCCGGTGCCGGAAATGTTCATCTTTTGTTCCCACCAGAGGGCCTTTTATATCGTTCCCTCACGCTGCCCAACGCCAAATACAAACTCa < 1:82878/139‑1 (MQ=255) cGGTACATACGCTCACGGACGATCTTCCGCTGGAGTCGCTGGCCGATCAGCCCGGTGCCGGAAATGTTCATCTTTTGTTCCCACCAGAGGGCCTTTTATATCGTTCCCTCACGCTGCCCAACGCCa > 1:626778/1‑126 (MQ=255) cGGTACATACGCTCACGGACGATCTTCCGCTGGAGTCGCTGGCCGATCAGCCCGGTGCCGGAAATGTTCATCTTTTGTTCCCACCAGAGGGCCTTTTATATCGTTCCCTCACGCTGCCCAACGCCa < 2:626778/126‑1 (MQ=255) cGGTACATACGCTCACGGACGATCTTCCGCTGGAGTCGCTGGCCGATCAGCCCGGTGCCGGAAATGTTCATCTTTTGTTCCCACCAGAGGGCCTTTTATATCGTTCCCTCACGCTGCCCAACGCCAAATACAAACTCAc > 1:577110/1‑139 (MQ=255) cGGTACATACGCTCACGGACGATCTTCCGCTGGAGTCGCTGGCCGATCAGCCCAGTGCCGGAAATGTTCATCTTTTGTTCCCACCaga < 2:891753/88‑1 (MQ=255) cGGTACATACGCTCACGGACGATCTTCCGCTGGAGTCGCTGGCCGATCAGCCCAGTGCCGGAAATGTTCATCTTTTGTTCCCACCaga > 1:891753/1‑88 (MQ=255) gTACATACGCTCACGGACGATCTTCCGCTGGAGTCGCTGGCCGATCAGCCCGGTGCCGGAAATGTTCATCTTTTGTTCCCACCAGAGGGCCTTTTATATCGTTCCCTCACGCTGCCCAACGCCAAATACAAACTCACCg > 1:932659/1‑139 (MQ=255) tACATACGCTCACGGACGATCTTCCGCTGGAGTCGCTGGCCGATCAGCCCGGTGCCGGAAATGTTCATCTTTTGTTCCCACCAGAGGGCCTTTTATATCGTTCCCTCACGCTGCCCAACGCCAAATACAAACTCACCGc < 1:932253/139‑1 (MQ=255) tACATACGCTCACGGACGATCTTCCGCTGGAGTCGCTGGCCGATCAGCCCGGTGCCGGAAATGTTCATCTTTTGTTCCCACCAGAGGGCCTTTTATATCGTTCCCTCACGCTGCCCAACGCCAAATACAAACTCACCGc < 1:326172/139‑1 (MQ=255) cATACGCTCACGGACGATCTTCCGCTGGAGTCGCTGGCCGATCAGCCCGGTGCCGGAAATGTTCATCTTTTGTTCCCACCAGAGGGCCTTTTATATCGTTCCCTCACGCTGCCCAACGCCAAATACAAACTCACCGcac < 1:1174368/139‑1 (MQ=255) cATACGCTCACGGACGATCTTCCGCTGGAGTCGCTGGCCGATCAGCCCGGTGCCGGAAATGTTCATC‑TTTGTTCCCACCAGAGGGCCTTTTATATCGTTCCCTCACGCTGCCCAACGCCAAATACAAACTCACCGcaca < 1:705788/139‑1 (MQ=255) aTACGCTCACGGACGATCTTCCGCTGGAGTCGCTGGCCGATCAGCCCGGTGCCGGAAATGTTCATCTTTTGTTCCCACCAGAGGGCCTTTTATATCGTTCCCTCACGCTGCCCAACGCCAAATACAAACTCACCGcaca < 2:577110/139‑1 (MQ=255) gCTCACGGACGATCTTCCGCTGGAGTCGCTGGCCGATCAGCCCGGTGCCGGAAATGTTCATCTTTTGTTCCCACCAGAGGGCCTTTTATATCGTTCCCTCACGCTGCCCAACGCCAAATACAAACTCACCGCACAAACa > 2:715939/1‑139 (MQ=255) gCTCACGGACGATCTTCCGCTGGAGTCGCTGGCCGATCAGCCCGGTGCCGGAAATGTTCATCTTTTGTTCCCACCAGAGGGCCTTTTATATCGTTCCCTCACGCTGCCCAACGCCAAATACAAACTCACCGCACAAACa > 2:436844/1‑139 (MQ=255) gCTCACGGACGATCTTCCGCTGGAGTCGCTGGCCGATCAGCCCGGTGCCGGAAATGTTCATCGTTTGTTCCCACCAGAGGGCCTTTTATATCGTTCCCTCACGCTGCCCAACGCCAAATACAAACTCACCACACAAACa > 1:717733/1‑139 (MQ=255) cACGGACGATCTTCCGCTGGAGTCGCTGGCCGATCAGCCCGGTGCCGGAAATGTTCATCTTTTGTTCCCACCAGAGGGCCTTTTATATCGTTCCCTCACGCTGCCCAACGCCAAATACAAACTCACCGCACAAACATTa > 2:1062192/1‑139 (MQ=255) cACGGACGATCTTCCGCTGGAGTCGCTGGCCGATCAGCCCGGTGCCGGAAATGTTCATCTTTTGTTCCCACCAGAGGGCCTTTTATATCGTTCCCTCACGCTACCCAACGCCAAATACAAACTCACCGCACAAACATTa > 1:387864/1‑139 (MQ=255) cGGACGATCTTCCGCTGGAGTCGCTGGCCGATCAGCCCGGTGCCGGAAATGTTCATCTTTTGTTCCCACCAGAGGGCCTTTTATATCGTTCCCTCACGCTGCCCAACGCCAAATACAAACTCACCGCACAAACATTACa < 2:649961/139‑1 (MQ=255) cGGACGATCTTCCGCTGGAGTCGCT‑GCCGATCAGCCCGGTGCCGGAAATGTTCATCTTTTGTTCCCACCAGAgg > 1:1179304/1‑74 (MQ=255) cGGACGATCTTCCGCTGGAGTCGCT‑GCCGATCAGCCCGGTGCCGGAAATGTTCATCTTTTGTTCCCACCAGAgg < 2:1179304/74‑1 (MQ=255) gaacTTCCGCTGGAGTCGCTGGCCGATCAGCCCGGTGCCGGAAATGTTCATCTTTTGTTCCCACCAGAGGGCCTTTTATATCGTTCCCTCACGCTGCCCAACGCCAAATACAAACTCACCGCACAAACATTACAAtggc > 1:1104722/4‑139 (MQ=255) gCTGGAGTCGCTGGCCGATCAGCCCGGTGCCGGAAATGTTCATCTTTTGTTCCCACCAGAGGGCCTTTTATATCGTTCCCTCACGCTGCCCAACGCCAAATACAAACTCACCGCACAAACATTACAATGGCTGGCagag < 2:1117909/139‑1 (MQ=255) gTCGCTGGCCGATCAGCCCGGTGCCGGAAATGTTCATCTTTTGTTCCCACCAGAGGGCCtt > 2:1148804/1‑61 (MQ=255) gTCGCTGGCCGATCAGCCCGGTGCCGGAAATGTTCATCTTTTGTTCCCACCAGAGGGCCtt < 1:1148804/61‑1 (MQ=255) gCTGGCCGATCAGCCCGGTGCCGGAAATGTTCATCTTTTGTTCCCACCAGAggg > 2:56696/1‑54 (MQ=255) gCTGGCCGATCAGCCCGGTGCCGGAAATGTTCATCTTTTGTTCCCACCAGAggg < 1:56696/54‑1 (MQ=255) aTCAGCCCGGTGCCGGAAATGTTCATCTTTTGTTCCCACCAGAGGGCCTTTTATATCGTTCCCTCACg > 2:361367/1‑68 (MQ=255) aTCAGCCCGGTGCCGGAAATGTTCATCTTTTGTTCCCACCAGAGGGCCTTTTATATCGTTCCCTCACg < 1:361367/68‑1 (MQ=255) gAAATGTTCATCTTTTGTTCCCACCAGAGGGCCTTTTATATCGTTCCCTCACGCTGCCCAACGCCAAATACAAACTCACCGCACAAACATTACAATGGCTGGCAGAGGAGACGTTACCCGACAACACCCAGGACTGGCa > 1:297287/1‑139 (MQ=255) gAAATGTTCATCTTTTGTTCCCACCAGAGGGCCTTTTATATCGTTCCCTCACGCTGCCCAACGCCAAATACAAACTCACCGCACAAACATTACAATGGCTGGCAGAGGAGACGTTACCCGACAACACCCAGGACTGGCa > 1:1041337/1‑139 (MQ=255) gTTCATCTTTTGTTCCCACCAGAGGGCCTTTTATATCGTTCCCTCACGCTGCCCAACGCCAAATACAAACTCACCGCACAAACATTACAATGGCTGGCAGAGGAGACGTTACCCGACAACACCCAGGACTGGCACTGGa < 2:1127813/139‑1 (MQ=255) ttCATCTTTTGTTCCCACCAGAGGGCCTTTTATATCGTTCCCTCACGCTGCCCAACGCCAAATACAAACTCACCGCACAAACATTACAATGGCTGGCAGAGGAGACGTTACCCGACAACACCCAGGACTGGCACTGGAc < 1:715939/139‑1 (MQ=255) aTCTTTTGTTCCTACCAGAGGGCCTTTTATATCGTTCCCTCACGCTGCCCAACGCCAAATACAAACTCACCGCACAAACATTACAATGGCTGGCAGAGGAGACGTTACCCGACAACACCCAGGACTGGCACTGGACGGt < 2:618940/139‑1 (MQ=255) tCTTTTGTTCCCACCAGAGGGCCTTTTATATCGTTCCCTCACGCTGCCCAACGCCAAATACAAACTCACCGCACAAACATTACAATGGCTGGCAGAGGAGACGTTACCCGACAACACCCAGGACTGGCACTGGACGGtt < 1:333853/139‑1 (MQ=255) tttGTTCCCACCAGAGGGCCTTTTATATCGTTCCCTCACGCTGCCCAACGCCAAATACAAACTCACCGCACAAACATTACAATGGCTGGCAgagg < 1:1183534/95‑1 (MQ=255) tttGTTCCCACCAGAGGGCCTTTTATATCGTTCCCTCACGCTGCCCAACGCCAAATACAAACTCACCGCACAAACATTACAATGGCTGGCAgagg > 2:1183534/1‑95 (MQ=255) | CTCGACGCTGCGTAAGCATTGGGAATGGATGACGTTCTCCGCAGATAGCGTATCTTCGGTACATACGCTCACGGACGATCTTCCGCTGGAGTCGCTGGCCGATCAGCCCGGTGCCGGAAATGTTCATCTTTTGATCCCACCAGAGGGCCTTTTATATCGTTCCCTCACGCTGCCCAACGCCAAATACAAACTCACCGCACAAACATTACAATGGCTGGCAGAGGAGACGTTACCCGACAACACCCAGGACTGGCACTGGACGGTT > NC_000913/3463954‑3464218 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |