Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,490,191	C→T	intergenic (‑11/+75)	argD ← / ← pabA	bifunctional acetylornithine aminotransferase and succinyldiaminopimelate aminotransferase/aminodeoxychorismate synthase, subunit II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,490,191	0	C	T	100.0%	124.8 / NA	36	intergenic (‑11/+75)	argD/pabA	bifunctional acetylornithine aminotransferase and succinyldiaminopimelate aminotransferase/aminodeoxychorismate synthase, subunit II
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/18);  total (18/18)

GCCTTGCTGATCCCAGATTCGGCTGCCCTGACCTTTTACCGGAATAAACTCTGCCGGAGCATAAATCGGCAGGATCACTTCATCGAAAGTCGCGCGTGTAATTGCTGTTTGTTCAATTGCCATCTCATGATCACCCTGTTACGCATAAACAAATGTGAAATTATAACCACAAAATATGCATAAAAAATCACTAAATGGCAATCAGAAATCAGCGATGCAGGAAATTAGCCAGCAGTTGATGTCCTTGTTCGCTAAGAATA  >  NC_000913/3490058‑3490317                                                                                                                                      |                                                                                                                               gccttgcTGATCCCAGATTCGGCTGCCCTGACCTTTTACCGGAATAAACTCTGCCGGAGCATAAATCGGCAGGATCACTTCATCGAAAGTCGCGCGTGTAATTGCTGTTTGTTCAATTGCCATCTCATGATCATCCTGt                                                                                                                           <  2:162880/139‑1 (MQ=255)     tgcTGATCCCAGATTCGGCTGCCCTGACCTTTTACCGGAATAAACTCTGCCGGAGCATAAATCGGCAGGATCACTTCATCGAAAGTCGCGCGTGTAATTGCTGTTTGTTCAATTGCCATCTCATGATCATCCTGTTACg                                                                                                                       >  1:655965/1‑139 (MQ=255)      gcTGATCCCAGATTCGGCTGCCCTGACCTTTTACCGGAATAAACTCTGCCGGAGCATAAATCGGCAGGATCACTTCATCGAAAGTCGCGCGTGTAATTGCTGTTTGTTCAATTGCCATCTCATGATCATCCTGTTACGc                                                                                                                      >  2:63384/1‑139 (MQ=255)      gcTGATCCCAGATTCGGCTGCCCTGACCTTTTACCGGAATAAACTCTGCCGGAGCATAAATCGGCAGGATCACTTCATCGAAAGTCGCGCGTGTAATTGCTGTTTGTTCAATTGCCATCTCATGATCATCCTGTTACGc                                                                                                                      <  2:652044/139‑1 (MQ=255)       cTGATCCCAGATTCGGCTGCCCTGACCTTTTACCGGAATAAACTCTGCCGGAGCATAAATCGGCAGGATCACTTCATCGAAAGTCGCGCGTGTAATTGCTGTTTGTTCAATTGCCATCTCATGATCATCCTGTTACGCa                                                                                                                     <  1:1025638/139‑1 (MQ=255)            cccAGATTCGGCTGCCCTGACCTTTTACCGGAATAAACTCTGCCGGAGCATAAATCGGCAGGATCACTTCATCGAAAGTCGCGCGTGTAATTGCTGTTTGTTCAATTGCCATCTCATGATCATCCTGTTACGCATAAAc                                                                                                                >  1:1039952/1‑139 (MQ=255)                 aTTCGGCTGCCCTGACCTTTTACCGGAATAAACTCTGCCGGAGCATAAATCGGCAGGATCACTTCATCGAAAGTCGCGCGTGTAATTGCTGTTTGTTCAATTGCCATCTCATGATCATCCTGTTACGCATAAACAAAtg                                                                                                           >  2:293223/1‑139 (MQ=255)                           ccTGACCTTTTACCGGAATAAACTCTGCCGGAGCATAAATCGGCAGGATCACTTCATCGAAAGTCGCGCGTGTAATTGCTGTTTGTTCAATTGCCATCTCATGATCATCCTGTTACGCATAAACAAATGTGAAATtata                                                                                                 >  2:373773/1‑139 (MQ=255)                             tGACCTTTTACCGGAATAAACTCTGCCGGAGCATAAATCGGCAGGATCACTTCATCGAAAGTCGCGCGTGTAATTGCTGTTTGTTCAATTGCCATCTCATGATCATCCTGTTACGCATAAACAAATGTGAAATTATAAc                                                                                               >  1:265322/1‑139 (MQ=255)                             tGACCTTTTACCGGAATAAACTCTGCCGGAGCATAAATCGGCAGGATCACTTCATCGAAAGTCGCGCGTGTAATTGCTGTTTGTTCAATTGCCATCTCATGATCATCCTGTTACGCATAAACAAATGTGAAATTATAAc                                                                                               <  1:63384/139‑1 (MQ=255)                                    ttACCGGAATAAACTCTGCCGGAGCATAAATCGGCAGGATCACTTCATCGAAAGTCGCGCGTGTAATTGCTGTTTGTTCAATTGCCATCTCATGATCATCCTGt                                                                                                                           <  1:94755/104‑1 (MQ=255)                                    ttACCGGAATAAACTCTGCCGGAGCATAAATCGGCAGGATCACTTCATCGAAAGTCGCGCGTGTAATTGCTGTTTGTTCAATTGCCATCTCATGATCATCCTGt                                                                                                                           >  2:94755/1‑104 (MQ=255)                                    ttACCGGAATAAACTCTGCCGGAGCATAAATCGGCAGGATCACTTCATCGAAAGTCGCGCGTGTAATTGCTGTTTGTTCAATTGCCATCTCATGATCATCCTGTTACGc                                                                                                                      >  1:305013/1‑109 (MQ=255)                                    ttACCGGAATAAACTCTGCCGGAGCATAAATCGGCAGGATCACTTCATCGAAAGTCGCGCGTGTAATTGCTGTTTGTTCAATTGCCATCTCATGATCATCCTGTTACGc                                                                                                                      <  2:305013/109‑1 (MQ=255)                                    ttACCGGAATAAACTCTGCCGGAGCATAAATCGGCAGGATCACTTCATCGAAAGTCGCGCGTGTAATTGCTGTTTGTTCAATTGCCATCTCATGATCATCCTGTTACGCATAAACAAAtg                                                                                                           >  2:803273/1‑120 (MQ=255)                                    ttACCGGAATAAACTCTGCCGGAGCATAAATCGGCAGGATCACTTCATCGAAAGTCGCGCGTGTAATTGCTGTTTGTTCAATTGCCATCTCATGATCATCCTGTTACGCATAAACAAAtg                                                                                                           <  1:803273/120‑1 (MQ=255)                                      aCCGGAATAAACTCTGCCGGGGCATAAATCGGCAGGATCACTTCATCGAAAGTCGCGCGTGTAATTGCTGTTTGTTCAATTGCCATCTCATGATCATCCTGTTACGCATAAACAAATGTGAAATTATAACCACAAAata                                                                                      >  1:126638/1‑139 (MQ=255)                                         ggAATAAACTCTGCCGGAGCATAAATCGGCAGGATCACTTCATCGAAAGTCGCGCGTGTAATTGCTGTTTGTTCAATTGCCATCTCATGATCATCCTGTTACGCATAAACAAATGTGAAATTATAACCACAAAATATGc                                                                                   >  2:156548/1‑139 (MQ=255)                                           aaTAAACTCTGCCGGAGCATAAATCGGCAGGATCACTTCATCGAAAGTCGCGCGTGTAATTGCTGTTTGTTCAATTGCCATCTCATGATCATCCTGTTACGCATAAACAAATGTGAAATTATAACCACAAAATATGCAt                                                                                 >  2:413896/1‑139 (MQ=255)                                                         gAGCATAAATCGGCAGGATCACTTCATCGAAAGTCGCGCGTGTAATTGCTGTTTGTTCAATTGCCATCTCATGATCATCCTGTTACGCATAAAc                                                                                                                >  2:1175591/1‑94 (MQ=255)                                                         gAGCATAAATCGGCAGGATCACTTCATCGAAAGTCGCGCGTGTAATTGCTGTTTGTTCAATTGCCATCTCATGATCATCCTGTTACGCATAAAc                                                                                                                <  1:1175591/94‑1 (MQ=255)                                                           gCATAAATCGGCAGGATCACTTCATCGAAAGTCGCGCGTGTAATTGCTGTTTGTTCAATTGCCATCTCATGATCATCCTGTTACGCATAAACAAATGTGAAATTATAACCACAAAATATGCATAAAAAATCACTAAATg                                                                 >  2:292541/1‑139 (MQ=255)                                                             aTAAATCGGCAGGATCACTTCATCGAAAGTCGCGCGTGTAATTGCTGTTTGTTCAATTGCCATCTCATGATCATCCTCTTACGCATAAATAAATGTGAAATTATTACCACCAAATATGCATAAAAAATCACTAAATGGc                                                               <  2:782794/139‑1 (MQ=255)                                                                    ggCAGGATCACTTCATCGAAAGTCGCGCGTGTAATTGCTGTTTGTTCAATTGCCATCTCATGATCATCCTGTTACGCATAAACAAATGTGAAATTATAACCACAAAATATGCATAAAAAATCACTAAATGGCAATCAGa                                                        >  2:242267/1‑139 (MQ=255)                                                                          aTCACTTCATCGAAAGTCGCGCGTGTAATTGCTGTTTGTTCAATTGCCATCTCATGATCATCCTGTTACGCATAAACAAATGTGAAATTATAACCACAAAATATGCATAAAAAATCACTAAATGGCAATCAGAAATCAg                                                  <  2:358024/139‑1 (MQ=255)                                                                           tCACTTCATCGAAAGTCGCGCGTGTAATTGCTGTTTGTTCAATTGCCATCTCATGATCATCCTGTTACGCATAAACAAATGTGAAATTATAACCACAAAATATGCATAAAAAATCACTAAATGGCAATCAGAAATCAGc                                                 <  1:373773/139‑1 (MQ=255)                                                                                 cATCGAAAGTCGCGCGTGTAATTGCTGTTTGTTCAATTGCCATCTCATGATCATCCTGTTACGCATAAACAAATGTGAAATTATAACCACAAAATATGCATAAAAAATCACTAAATGGCAATCAGAAATCAGCGATGCa                                           <  1:242267/139‑1 (MQ=255)                                                                                           cgcgcgTGTAATTGCTGTTTGTTCAATTGCCATCTCATGATCATCCTGTTACGCATAAACAAATGTGAAATTATAACCACAAAATATGCATAAAAAATCACTAAATGGCAATCAGAAATCAGCGATGCAGGAAATTAGc                                 <  1:413896/139‑1 (MQ=255)                                                                                             cgcgTGTAATTGCTGTTTGTTCAATTGCCATCTCATGATCATCCTGTTACGCATAAACAAATGTGAAATTATAACCACAAAATATGCATAAAAAATCACTAAATGGCAATCAGAAATCAGCGATGCAGGAAATTAGCca                               >  2:998149/1‑139 (MQ=255)                                                                                                  gtAATTGCTGTTTGTTCAATTGCCATCTCATGATCATCCTGTTACGCATAAACAAATGTGAAATTATAACCACAAAATATGCATAAAAAATCACTAAATGGCAATCAGAAATCAGCGATGCAGGAAATTAGCCAGCAGt                          <  1:156548/139‑1 (MQ=255)                                                                                                     aTTGCTGTTTGTTCAATTGCCATCTCATGATCATCCTGTTACGCATAAACAAATGTGAAATTATAACCACAAAATATGCATAAAAAATCACTAAATGGCAATCAGAAATCAGCGATGCAGGAAATTAGCCAGCAGTTGa                       <  1:998149/139‑1 (MQ=255)                                                                                                              tgttCAATTGCCATCTCATGATCATCCTGTTACGCATAAACAAATGTGAAATTATAACCACAAAATATGCATAAAAAATCACTAAATGGCAATCAGAAATCAGCGATGCAGGAAATTAGCCAGCAGTTGATGTCCTTGt              >  2:726014/1‑139 (MQ=255)                                                                                                                ttCAATTGCCATCTCATGATCATCCTGTTACGCATAAACAAATGTGAAATTATAACCACAAAATATGCATAAAAAATCACTAAATGGCAATCAGAAATCAGCGATGCAGGAAATTAGCCAGCAGTTGATGTCCTTGTTc            <  2:126638/139‑1 (MQ=255)                                                                                                                    aTTGCCATCTCATGATCATCCTGTTACGCATAAACAAATGTGAAATTATAACCACAAAATATGCATAAAAAATCACTAAATGGCAATCAGAAATCAGCGATGCAGGAAATTAGCCAGCAGTTGATGTCCTTGTTCGCTa        <  2:766566/139‑1 (MQ=255)                                                                                                                         cATCTCATGATCATCCTGTTACGCATAAACAAATGTGAAATTATAACCACAAAATATGCATAAAAAATCACTAAATGGCAATCAGAAATCAGCGATGCAGGAAATTAGCCAGCAGTTGATGTCCTTGTTCGCTAAGAAt   <  2:1039952/139‑1 (MQ=255)                                                                                                                          aTCTCATGATCATCCTGTTACGCATAAACAAATGTGAAATTATAACCACAAAATATGCATAAAAAATCACTAAATGGAAATCAAAAATAAGCAATAAAGGAAATAAGCCAGCAGTTAATGTCCTTGTTCGCTAAAAATa  >  2:746349/1‑139 (MQ=255)                                                                                                                                      |                                                                                                                               GCCTTGCTGATCCCAGATTCGGCTGCCCTGACCTTTTACCGGAATAAACTCTGCCGGAGCATAAATCGGCAGGATCACTTCATCGAAAGTCGCGCGTGTAATTGCTGTTTGTTCAATTGCCATCTCATGATCACCCTGTTACGCATAAACAAATGTGAAATTATAACCACAAAATATGCATAAAAAATCACTAAATGGCAATCAGAAATCAGCGATGCAGGAAATTAGCCAGCAGTTGATGTCCTTGTTCGCTAAGAATA  >  NC_000913/3490058‑3490317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑