Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,702,561	A→T	I103N (ATC→AAC)	dppF ←	dipeptide/heme ABC transporter ATPas

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,702,561	0	A	T	100.0%	92.7 / NA	28	I103N (ATC→AAC)	dppF	dipeptide/heme ABC transporter ATPas
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (13/15);  total (13/15)

CTTTCGCCATCATCGACAGGGCTTTTTCCCGACGCTGTTCTTTGCTTAAGCTGGTGTTGATCAGCAGCGGCTCTTCAAGAATTTGCCCGACTTTTTTACGCGGATTCAGCGAACCGTAAGGGTTCTGGAAGACGATCTGGATTTTCTGCCGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTCGCCACCGGTGGGCATTTCAATCATCGTCAGCAACCGACCGAGGGTCGATTTACC  >  NC_000913/3702427‑3702691                                                                                                                                       |                                                                                                                                   cTTTCGCCATCATCGACAGGGCTTTTTCCCGACGCTGTTCTTTGCTTAAGCTGGTGTTGATCAGCAGCGGCTCTTCAAGAATTTGCCCGACTTTTTTACGCGGATTCAGCGAACCGTAAGGGTTCTGGAAGACGTTCTg                                                                                                                                >  2:1134034/1‑139 (MQ=255)                              cGACGCTGTTCTTTGCTTAAGCTGGTGTTGATCAGCAGCGGCTCTTCAAGAATTTGCCCGACTTTTTTACGCGGATTCAGCGAACCGTAAGGGTTCTGGAAGACGTTCTGGATTTTCTGCCGACGCAGCTTCTGCGCCt                                                                                                   <  2:287251/139‑1 (MQ=255)                                    tGTTCTTTGCTTAAGCTGGTGTTGATCAGCAGCGGCTCTTCAAGAATTTGCCCGACTTTTTTACGCGGATTCAGCGAACCGTAAGGGTTCTGGAAGACGTTCTGGATTTTCTGCCGACGCAGCTTCTGCGCCTGCGGAt                                                                                             <  1:497253/139‑1 (MQ=255)                                    tGTTCTTTGCTTAAGCTGGTGTTGATCAGCAGCGGCTCTTCAAGAATTTGCCCGACTTTTTTACGCGGATTCAGCGAACCGTAAGGGTTCTGGAAGACGTTCTGGATTTTCTGCCGACGCAGCTTCTGCGCCTGCGGAt                                                                                             <  1:503292/139‑1 (MQ=255)                                      ttCTTTGCTTAAGCTGGTGTTGATCAGCAGCGGCTCTTCAAGAATTTGCCCGACTTTTTTACGCGGATTCAGCGAACCGTAAGGGTTCTGGAAGACGTTCTGGATTTTCTGCCGACGCAGCTTCTGCGCCTGCGGAt                                                                                             <  2:599318/137‑1 (MQ=255)                                      ttCTTTGCTTAAGCTGGTGTTGATCAGCAGCGGCTCTTCAAGAATTTGCCCGACTTTTTTACGCGGATTCAGCGAACCGTAAGGGTTCTGGAAGACGTTCTGGATTTTCTGCCGACGCAGCTTCTGCGCCTGCGGAt                                                                                             >  1:599318/1‑137 (MQ=255)                                           tGCTTAAGCTGGTGTTGATCAGCAGCGGCTCTTCAAGAATTTGCCCGACTTTTTTACGCGGATTCAGCGAACCGTAAGGGTTCTGGAAGACGTTCTGGATTTTCTGCCGACGCAGCTTCTGCGCCTGCGGATCGTGCtt                                                                                      >  1:436432/1‑139 (MQ=255)                                                         ttGATCAGCAGCGGCTCTTCAAGAATTTGCCCGACTTTTTTACGCGGATTCAGCGAACCGTAAGGGTTCTGGAAGACGTTCTGGATTTTCTGCCGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGcc                                                                        <  2:130154/139‑1 (MQ=255)                                                          tGATCAGCAGCGGCTCTTCAAGAATTTGCCCGACTTTTTTACGCGGATTCAGCGAACCGTAAGGGTTCTGGAAGACGTTCTGGATTTTCTGCCGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGccc                                                                       <  2:33757/139‑1 (MQ=255)                                                                gcagcGGCTCTTCAAGAATTTGCCCGACTTTTTTACGCGGATTCAGCGAACCGTAAGGGTTCTGGAAGACGTTCTGGATTTTCTGCCGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGcc                                                                        >  1:1086870/1‑132 (MQ=255)                                                                gcagcGGCTCTTCAAGAATTTGCCCGACTTTTTTACGCGGATTCAGCGAACCGTAAGGGTTCTGGAAGACGTTCTGGATTTTCTGCCGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGcc                                                                        <  2:1086870/132‑1 (MQ=255)                                                                gcagcGGCTCTTCAAGAATTTGCCCGACTTTTTTACGCGGATTCAGCGAACCGTAAGGGTTCTGGAAGACGTTCTGGATTTTCTGCCGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTa                                                                 >  2:391380/1‑139 (MQ=255)                                                                      gCTCTTCAAGAATTTGCCCGACTTTTTTACGCGGATTCAGCGAACCGTAAGGGTTCTGGAAGACGTTCTGGATTTTCTGCCGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTAATACAg                                                           >  1:656482/1‑139 (MQ=255)                                                                                    tGCCCGACTTTTTTACGCGGATTCAGCGAACCGTAAGGGTTCTGGAAGACGTTCTGGATTTTCTGCCGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTCGCCACCGGTgg                                             >  2:1029220/1‑139 (MQ=255)                                                                                    tGCCCGACTTTTTTACGCGGATTCAGCGAACCGTAAGGGTTCTGGAAGACGTTCTGGATTTTCTGCCGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTCGCCACCGGTgg                                             <  2:274555/139‑1 (MQ=255)                                                                                     gCCCGACTTTTTTACGCGGATTCAGCGAACCGTAAGGGTTCTGGAAGACGTTCTGGATTTTCTGCCGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTCGCCACCGGTggg                                            <  1:617826/139‑1 (MQ=255)                                                                                             tttttACGCGGATTCAGCGAACCGTAAGGGTTCTGGAAGACGTTCTGGATTTTCTGCCGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTCGCCACCGGTGGGCATTTCaa                                    <  2:944072/139‑1 (MQ=255)                                                                                                    gcgGATTCAGCGAACCGTAAGGGTTCTGGAAGACGTTCTGGATTTTCTGCCGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTCGCCACCGGTGGGCATTTCAATCATCGt                             <  1:864997/139‑1 (MQ=255)                                                                                                           cAGCGAACCGTAAGGGTTCTGGAAGACGTTCTGGATTTTCTGCCGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTCGCCACCGGTGGGCATTTCAATCATCGTCAGCAac                      <  1:561929/139‑1 (MQ=255)                                                                                                                aaCCGTAAGGGTTCTGGAAGACGTTCTGGATTTTCTGCCGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTCGCCACCGGTGGGCATTTCAATCATCGTCAGCAaccgacc                 <  2:670299/139‑1 (MQ=255)                                                                                                                 aCCGTAAGGGTTCTGGAAGACGTTCTGGATTTTCTGCCGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTCGCCACCGGTGGGCATTTCAATCATCGTCAGCAaccgaccg                <  2:656482/139‑1 (MQ=255)                                                                                                                  ccGTAAGGGTTCTGGAAGACGTTCTGGATTTTCTGCCGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTCGCCACCGGTGGGCATTTCa                                     <  1:678398/117‑1 (MQ=255)                                                                                                                  ccGTAAGGGTTCTGGAAGACGTTCTGGATTTTCTGCCGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTCGCCACCGGTGGGCATTTCa                                     >  2:678398/1‑117 (MQ=255)                                                                                                                  ccGTAAGGGTTCTGGAAGACGTTCTGGATTTTCTGCCGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTCGCCACCGGTGGGCATTTCAATCATCGTCAGCAAccgaccga               >  2:975526/1‑139 (MQ=255)                                                                                                                             cTGGAAGACGTTCTGGATTTTCTGCCGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTCGCCACCGGTGGGCATTTCAATCATCTTCAGCAACCGACCGAGGGTCGATTTa    >  2:666401/1‑139 (MQ=255)                                                                                                                             cTGGAAGACGTTCTGGATTTTCTGCCGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTCGCCACCGGTGGGCATTTCAATCATCGTCAGCAACCGACCGAGGGTCGATTTa    >  1:702558/1‑139 (MQ=255)                                                                                                                               ggAAGACGTTCTGGATTTTCTGCCGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTCGCCACCGGTGGGCATTTCAATCATCGTCAGCAACCGACCGAGGGTCGATTTAcc  >  2:946455/1‑139 (MQ=255)                                                                                                                                    aCGTTCTGGATTTTCTGCCGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTG‑CCCTGGTAATACAGCTCGCCACCGGTGGGCATTTCAATCATCGTCAGCAACCGACCGAGGGTCGATTTAcc  >  1:404231/1‑133 (MQ=255)                                                                                                                                       |                                                                                                                                   CTTTCGCCATCATCGACAGGGCTTTTTCCCGACGCTGTTCTTTGCTTAAGCTGGTGTTGATCAGCAGCGGCTCTTCAAGAATTTGCCCGACTTTTTTACGCGGATTCAGCGAACCGTAAGGGTTCTGGAAGACGATCTGGATTTTCTGCCGACGCAGCTTCTGCGCCTGCGGATCGTGCTTAAGCAGATCCTGCCCCTGGTAATACAGCTCGCCACCGGTGGGCATTTCAATCATCGTCAGCAACCGACCGAGGGTCGATTTACC  >  NC_000913/3702427‑3702691

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑