Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,791,004 A→G G114G (GGT→GGC)  tdh ← L‑threonine 3‑dehydrogenase, NAD(P)‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,791,0040AG100.0% 207.0 / NA 56G114G (GGT→GGC) tdhL‑threonine 3‑dehydrogenase, NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/26);  total (30/26)

ACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCTGACCAATACCTAC  >  NC_000913/3790868‑3791138
                                                                                                                                        |                                                                                                                                      
aCGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGcc                                                                                                                                      >  1:887310/1‑139 (MQ=255)
  gACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCaa                                                                                                                                    >  1:656289/1‑139 (MQ=255)
      gcgcGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTaacgccaacgcc                                                                                                                                >  2:128651/1‑139 (MQ=255)
       cgcgGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAAc                                                                                                                                   <  1:1117077/135‑1 (MQ=255)
       cgcgGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAAc                                                                                                                                   >  2:1117077/1‑135 (MQ=255)
              tGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCgtgt                                                                                                                        <  2:344697/139‑1 (MQ=255)
                     cGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGca                                                                                                                 <  1:410615/139‑1 (MQ=255)
                       ttACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGcaca                                                                                                               <  2:656289/139‑1 (MQ=255)
                        tACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACaa                                                                                                              >  2:1064300/1‑139 (MQ=255)
                                    cAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGacc                                                                                                  >  1:930143/1‑139 (MQ=255)
                                          ttGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACg                                                                                            >  1:42594/1‑139 (MQ=255)
                                           tGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGc                                                                                                                                 <  2:674861/101‑1 (MQ=255)
                                           tGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGc                                                                                                                                 >  1:674861/1‑101 (MQ=255)
                                           tGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGa                                                                                           >  1:1098533/1‑139 (MQ=255)
                                            gCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACg                                                                                                     >  1:499788/1‑128 (MQ=255)
                                            gCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACg                                                                                                     <  2:499788/128‑1 (MQ=255)
                                            gCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGAc                                                                                          <  2:306140/139‑1 (MQ=255)
                                            gCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGAc                                                                                          >  1:694021/1‑139 (MQ=255)
                                                            aaaTATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGAcc                                                                          <  2:1001166/139‑1 (MQ=255)
                                                            aaaTATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGAcc                                                                          <  1:445539/139‑1 (MQ=255)
                                                             aaTATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACca                                                                         <  1:502717/139‑1 (MQ=255)
                                                                   gTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGt                                                                                                        >  2:421909/1‑102 (MQ=255)
                                                                   gTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGt                                                                                                        <  1:421909/102‑1 (MQ=255)
                                                                      ggggATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACg                                                                                                     >  2:951091/1‑102 (MQ=255)
                                                                      ggggATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACg                                                                                                     <  1:951091/102‑1 (MQ=255)
                                                                          aTTTTGAAGGGGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCg                                                                                                                    >  1:1153061/1‑83 (MQ=255)
                                                                          aTTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCg                                                                                                                    <  2:1153061/83‑1 (MQ=255)
                                                                                      ttGAATGCCGGGATCGCCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCa                                                                                                                                     >  2:807447/1‑54 (MQ=255)
                                                                                      ttGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCa                                                                                                                                     <  1:807447/54‑1 (MQ=255)
                                                                                        gAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAAcg                                              <  1:1064300/139‑1 (MQ=255)
                                                                                          aTGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAAcgcg                                            <  1:45636/139‑1 (MQ=255)
                                                                                               gggATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGc                                       <  2:802174/139‑1 (MQ=255)
                                                                                                        aGATATTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGa                              <  1:535234/139‑1 (MQ=255)
                                                                                                            atTCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGcc                          >  1:546127/1‑139 (MQ=255)
                                                                                                             ttCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCt                         >  1:693114/1‑139 (MQ=255)
                                                                                                              tCGGCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCtt                        >  2:268249/1‑139 (MQ=255)
                                                                                                                 gCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCgg                                                                                                                   >  2:424941/1‑45 (MQ=255)
                                                                                                                 gCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCgg                                                                                                                   <  1:424941/45‑1 (MQ=255)
                                                                                                                 gCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGac                                                                                                   >  1:646549/1‑61 (MQ=255)
                                                                                                                 gCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGac                                                                                                   <  2:646549/61‑1 (MQ=255)
                                                                                                                 gCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCa                     >  1:214164/1‑139 (MQ=255)
                                                                                                                 gCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCa                     >  2:588169/1‑139 (MQ=255)
                                                                                                                 gCAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCG‑TCTTGAAGCCTTTCAc                    >  2:174669/1‑139 (MQ=255)
                                                                                                                  cAAAGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCAc                    >  1:528541/1‑139 (MQ=255)
                                                                                                                    aaGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACaa                                                                                                              >  1:663952/1‑47 (MQ=255)
                                                                                                                    aaGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACaa                                                                                                              <  2:663952/47‑1 (MQ=255)
                                                                                                                    aaGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACtt                  >  1:749952/1‑139 (MQ=255)
                                                                                                                    aaGCAGCCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACtt                  >  1:320633/1‑139 (MQ=255)
                                                                                                                     agcagcCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACaa                                                                                                              >  1:83559/1‑46 (MQ=255)
                                                                                                                     agcagcCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACaa                                                                                                              <  2:83559/46‑1 (MQ=255)
                                                                                                                     agcagcCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAAt                                                                                                            <  2:467116/48‑1 (MQ=255)
                                                                                                                     agcagcCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAAt                                                                                                            >  1:467116/1‑48 (MQ=255)
                                                                                                                     agcagcCCGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTc                 <  2:693114/139‑1 (MQ=255)
                                                                                                                          cccGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCTGAc            <  2:528541/139‑1 (MQ=255)
                                                                                                                            cGGGCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCTGACCa          <  2:320633/139‑1 (MQ=255)
                                                                                                                              ggCGATTAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCTGACCAAt        >  2:470546/1‑139 (MQ=255)
                                                                                                                                    tAACGCCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCTGACCAAtacctac  >  1:451588/1‑139 (MQ=255)
                                                                                                                                        |                                                                                                                                      
ACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCTGACCAATACCTAC  >  NC_000913/3790868‑3791138

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑