Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	509,678	T→A	I568F (ATC→TTC)	copA ←	copper transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	509,678	0	T	A	100.0%	59.2 / NA	18	I568F (ATC→TTC)	copA	copper transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (9/9);  total (9/9)

ATTGCCCAGCAATAACGCATGACCTTCAGCTTCACCGCTCACGCCCAGCCCGCGCAATGTGCGGAAACCGTTGACCTGCGGTAGCTGCATATCACCTGCTTTATCGAGGATCGCTCGTGCCAGCGGATGGCTGGAACCTTGCTCCAGTGCCGCCGCCAGACGCAATGCCTGCGCTTCATCAACATCAGCAAATGTTTTCACTGCGACAACCTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTA  >  NC_000913/509568‑509811                                                                                                               |                                                                                                                                      aTTGCCCAGCAATAACGCATGACCTTCAGCTTCACCGCTCACGCCCAGCCCGCGCAATGTGCGGAAACCGTTGACCTGCGGTAGCTGCATATCACCTGCTTTATCGAGGAACGCTCGTGCCAGCGGATGGCTGGAACCt                                                                                                           <  1:124865/139‑1 (MQ=255)                          tCAGCTTCACCGCTCACGCCCAGCCCGCGCAATGTGCGGAAACCGTTGACCTGCGGTAGCTGCATATCACCTGCTTTATCGAGGAACACTCGTGCCAGCGGATGGCTGGAACCTTGCTCCAGTGCCGCCGCCAGACGCa                                                                                  >  2:287032/1‑139 (MQ=255)                                   ccGCTCACGCCCAGCCCGCGCAATGTGCGGAAACCGTTGACCTGCGGTAGCTGCATATCACCTGCTTTATCGAGGAACGCTCGTGCCAGCGGATGGCTGGAACCTTGCTCCAGTGCCGCCGCCAGACGCAATGCCTgcg                                                                         <  2:1041962/139‑1 (MQ=255)                                   ccGCTCACGCCCAGCCCGCGCAATGTGCGGAAACCGTTGACCTGCGGTAGCTGCATATCACCTGCTTTATCGAGGAACGCTCGTGCCAGCGGATGGCTGGAACCTTGCTCCAGTGCCGCCGCCAGACGCAATGCCTgcg                                                                         <  2:413798/139‑1 (MQ=255)                                        cACGCCCAGCCCGCGCAATGTGCGGAAACCGTTGACCTGCGGTAGCTGCATATCACCTGCTTTATCGAGGAACGCTCGTGCCAGCGGATGGCTGGAACCTTGCTCCAGTGCCGCCGCCAGACGCAATGCCTGCGCTtca                                                                    <  2:1106745/139‑1 (MQ=255)                                             ccAGCCCGCGCAATGTGCGGAAACCGTTGACCTGCGGTAGCTGCATATCACCTGCTTTATCGAGGAACGCTCGTGCCAGCGGATGGCTGGAACCTTGCTCCAGTGCCGCCGCCAGACGCAATGCCTGCGCTTCATCAAc                                                               <  1:594583/139‑1 (MQ=255)                                                    gcgcAATGTGCGGAAACCGTTGACCTGCGGTAGCTGCATATCACCTGCTTTATCGAGGAACGCTCGTGCCAGCGGATGGCTGGAACCTTGCTCCAGTGCCGCCGCCAGACGCAATGCCTGCGCTTCATCAACATCAGCa                                                        <  1:364786/139‑1 (MQ=255)                                                     cgcAATGTGCGGAAACCGTTGACCTGCGGTAGCTGCATATCACCTGCTTTATCGAGGAACGCTCGTGCCAGCGGCTGGCTGGAACCTTGCTCCAGTGCCGCCGCCAAACGCAATGCCTGCGCTTCATCAACATCAGCaa                                                       >  2:904517/1‑139 (MQ=255)                                                               ggAAACCGTTGACCTGCGGTAGCTGCATATCACCTGCTTTATCGAGGAACGCTCGTGCCAGCGGATGGCTGGAACCTTGCTCCAGTGCCGCCGCCAGACGCAATGCCTGCGCTTCATCAACATCAGCAAATGTTTTCAc                                             >  2:1182748/1‑139 (MQ=255)                                                                       ttGACCTGCGGTAGCTGCATATCACCTGCTTTATCGAGGAACGCTCGTGCCAGCGGATGGCTGGAACCTTGCTCCAGTGCCGCCGCCAGACGCAATGCCTGCGCTTCATCAACATCAGCAAATGTTTTCAc                                             <  1:670483/131‑1 (MQ=255)                                                                       ttGACCTGCGGTAGCTGCATATCACCTGCTTTATCGAGGAACGCTCGTGCCAGCGGATGGCTGGAACCTTGCTCCAGTGCCGCCGCCAGACGCAATGCCTGCGCTTCATCAACATCAGCAAATGTTTTCAc                                             >  2:670483/1‑131 (MQ=255)                                                                                ggTAGCTGCATATCACCTGCTTTATCGAGGAACGCTCGTGCCAGCGGATGGCTGGAACCTTGCTCCAGTGCCGCCGCCAGACGCAATGCCTGCGCTTCATCAACATCAGCAAATGTTTTCACTGCGACAACCTGCGGCt                            <  1:278026/139‑1 (MQ=255)                                                                                            tCACCTGCTTTATCGAGGAACGCTCGTGCCAGCGGATGGCTGGAACCTTGCTCCAGTGCCGCCGCCAGACGCAATGCCTGCGCTTCATCAACATCAGCAAATGTTTTCACTGCGACAACCTGCGGCTTCCCTtcagtca                >  2:436236/1‑139 (MQ=255)                                                                                               ccTGCTTTATCGAGGAACGCTCGTGCCAGCGGATGGCTGGAACCTTGCTCCAGTGCCGCCGCCAGACGCAATGCCTGCGCTTCATCAACATCAGCAAATGTTTTCACTGCGACAACCTGCGGCTTCCCTTCAGTCAGCg             >  1:138495/1‑139 (MQ=255)                                                                                                  gCTTTATCGAGGAACGCTCGTGCCAGCGGATGGCTGGAACCTTGCT‑CAGTGCCGCCGCCAGACGCAATGCCTgcg                                                                         <  2:97878/75‑1 (MQ=255)                                                                                                  gCTTTATCGAGGAACGCTCGTGCCAGCGGATGGCTGGAACCTTGCT‑CAGTGCCGCCGCCAGACGCAATGCCTgcg                                                                         >  1:97878/1‑75 (MQ=255)                                                                                                    tttATCGAGGAACGCTCGTGCCAGCGGATGGCTGGAACCTTGCTCCAGTGCCGCCGCCAGACGCAATGCCTGCGCTTCATCAACATCAGCAAATGTTTTCACTGCGACAACCTGCGGCTTCCCTTCAGTCAGCGTCCCg        >  2:1160452/1‑139 (MQ=255)                                                                                                          gAGGAACGCTCGTGCCAGCGGATGGCTGGAACCTTGCTCCAGTGCCGCCGCCAGACGCAATGCCTGCGCTTCATCAACATCAGCAAATGTTTTCACTGCGACAACCTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTa  >  2:476111/1‑139 (MQ=255)                                                                                                               |                                                                                                                                      ATTGCCCAGCAATAACGCATGACCTTCAGCTTCACCGCTCACGCCCAGCCCGCGCAATGTGCGGAAACCGTTGACCTGCGGTAGCTGCATATCACCTGCTTTATCGAGGATCGCTCGTGCCAGCGGATGGCTGGAACCTTGCTCCAGTGCCGCCGCCAGACGCAATGCCTGCGCTTCATCAACATCAGCAAATGTTTTCACTGCGACAACCTGCGGCTTCCCTTCAGTCAGCGTCCCGGTTTTA  >  NC_000913/509568‑509811

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑