Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,459,820	A→G	pseudogene (331/366 nt)	cybC →	pseudogene, truncated cytochrome b562, cytochrome b(562)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,459,820	0	A	G	100.0%	180.4 / NA	47	pseudogene (331/366 nt)	cybC	pseudogene, truncated cytochrome b562, cytochrome b(562)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/26);  total (21/26)

TCACCGGACAGCCCGGAAATGAAAGATTTCCGCCACGGTTTCGACATTCTGGTCGGTCAGATTGACGACGCGCTGAAGCTGGCAAATGAAGGTAAAGTAAAAGAAGCGCAGGCTGCTGCAGAGCAACTGAAAACGACCCGCAATTCATATCACAAGAAGTATCGTTAATTCCTCATTTCCCTGTTGCCTGCACTCAGGTAACAGGGAACTTATCATCGCAAATGATGCACCACCTGATTGCTGCTGCCGCGCCAGATCAGGGAAGGGT  >  NC_000913/4459688‑4459955                                                                                                                                     |                                                                                                                                        tCACCGGACAGCCCGGAAATGAAAGATTTCCGCCACGGTTTCGACATTCTGGTCGGTCAGATTGACGACGCGCTGAAGCTGGCAAATGAAGGTAAAGTAAAAGAAGCGCAGGCTGCTGCAGAGCAACTGAAAGCGAccc                                                                                                                                   <  1:805879/139‑1 (MQ=255)     cGGACAGCCCGGAAATGAAAGATTTCCGCCACGGTTTCGACATTCTGGTCGGTCAGATTGACGACGCGCTGAAGCTGGCAAATGAAGGTAAAGTAAAAGAAGCGCAGGCTGCTGCAGAGCAACTGAAAGCGACCCGCaa                                                                                                                               >  2:571030/1‑139 (MQ=255)           gCCCGGAAATGAAAGATTTCCGCCACGGTTTCGACATTCTGGTCGGTCAGATTGACGACGCGCTGAAGCTGGCAAATGAAGGTAAAGTAAAAGAAGCGCAGGCTGCTGCAGAGCAACTGAAAGCGACCCGCAATTCata                                                                                                                         <  1:182973/139‑1 (MQ=255)            cccGGAAATGAAAGATTTCCGCCACGGTTTCGACATTCTGGTCGGTCAGATTGACGACGCGCTGAAGCTGGCAAATGAAGGTAAAGTAAAAGAAGCGCAGGCTGCTGCAGAGCAACTGAAAGCGACCCGCAATTCatat                                                                                                                        <  2:506089/139‑1 (MQ=255)            cccGGAAATGAAAGATTTCCGCCACGGTTTCGACATTCTGGTCGGTCAGATTGACGACGCGCTGAAGCTGGCAAATGAAGGTAAAGTAAAAGAAGCGCAGGCTGCTGCAGAGCAACTGAAAGCGACCCGCAATTCatat                                                                                                                        >  1:625839/1‑139 (MQ=255)            cccGGAAATGAAAGATTTCCGCCACGGTTTCGACATTCTGGTCGGTCAGATTGACGACGCGCTGAAGCTGGCAAATGAAGGTAAAGTAAAAGAAGCGCAGGCTGCTGCAGAGCAACTGAAAGCGACCCGCAATTCat                                                                                                                          <  1:650003/137‑1 (MQ=255)            cccGGAAATGAAAGATTTCCGCCACGGTTTCGACATTCTGGTCGGTCAGATTGACGACGCGCTGAAGCTGGCAAATGAAGGTAAAGTAAAAGAAGCGCAGGCTGCTGCAGAGCAACTGAAAGCGACCCGCAATTCat                                                                                                                          >  2:650003/1‑137 (MQ=255)                      aaaGATTTCCGCCACGGTTTCGACATTCTGGTCGGTCAGATTGACGACGCGCTGAAGCTGGCAAATGAAGGTAAAGTAAAAGAAGCGCAGGCTGCTGCAGAGCAACTGAAAGCGACCCGCAATTCATATCACAAGAAGt                                                                                                              >  2:214189/1‑139 (MQ=255)                      aaaGATTTCCGCCACGGTTTCGACATTCTGGTCGGTCAGATTGACGACGCGCTGAAGCTGGCAAATGAAGGTAAAGTAAAAGAAGCGCAGGCTGCTGCAGAGCAACTGAAAGCGACCCGCAATTCATATCACAAGAAGt                                                                                                              >  1:265002/1‑139 (MQ=255)                                       tttCGACATTCTGGTCGGTCAGATTGACGACGCGCTGAAGCTGGCAAATGAAGGTAAAGTAAAAGAAGCGCAGGCTGCTGCAGAGCAACTGAAAGCGACCCGCAATTCATATCACAAGAAGTATCGTTAATTCCTCAtt                                                                                             <  1:1067944/139‑1 (MQ=255)                                       tttCGACATTCTGGTCGGTCAGATTGACGACGCGCTGAAGCTGGCAAATGAAGGTAAAGTAAAAGAAGCGCAGGCTGCTGCAGAGCAACTGAAAGCGACCCGCAATTCATATCACAAGAAGTATCGTTAATTCCTCAtt                                                                                             <  1:1121125/139‑1 (MQ=255)                                            aCATTCTGGTCGGTCAGATTGACGACGCGCTGAAGCTGGCAAATGAAGGTAAAGTAAAAGAAGCGCAGGCTGCTGCAGAGCAACTGAAAGCGACCCGCAATTCATATCACAAGAAGTATCGTTAATTCCTCATTTCCCt                                                                                        <  1:379916/139‑1 (MQ=255)                                                         tcAGATTGACGACGCGCTGAAGCTGGCAAATGAAGGTAAAGTAAAAGAAGCGCAGGCTGCTGCAGAGCAACTGAAAGCGACCCGCAATTCATATCACAAGAAGTATCGTTAATTCCTCATTTCCCTGTTGCCTGCACTc                                                                           <  2:265002/139‑1 (MQ=255)                                                         tcAGATTGACGACGCGCTGAAGCTGGCAAATGAAGGTAAAGTAAAAGAAGCGCAGGCTGCTGCAGAGCAACTGAAAGCGACCCGCAATTCATATCACAAGAAGTATCGTTAATTCCTCATTTCCCTGTTGCCTGCACTc                                                                           >  1:916950/1‑139 (MQ=255)                                                         tcAGATTGACGACGCGCTGAAGCTGGCAAATGAAGGTAAAGTAAAAGAAGCGCAGGCTGCTGCAGAGCAACTGAAAGCGACCCGCAATTCATATCACAAGAAGTATCGTTAATTCCTCATTTCCCTGTTGCCTGCACTc                                                                           <  2:214049/139‑1 (MQ=255)                                                            gATTGACGACGCGCTGAAGCTGGCAAATGAAGGTAAAGTAAAAGAAGCGCAGGCTGCTGCAGTGCAACTGAAAGCGACCCGCAATTCCTATCACAAGAAGTATCGTTAATTCCTTATTTCCCTGTTGCCTGTACTCAgg                                                                        <  1:739276/139‑1 (MQ=255)                                                            gATTGACGACGCGCTGAAGCTGGCAAATGAAGGTAAAGTAAAAGAAGCGCAGGCTGCTGCAGAGCAACTGAAAGCGACCCGCAATTCATATcac                                                                                                                     >  1:297726/1‑94 (MQ=255)                                                            gATTGACGACGCGCTGAAGCTGGCAAATGAAGGTAAAGTAAAAGAAGCGCAGGCTGCTGCAGAGCAACTGAAAGCGACCCGCAATTCATATcac                                                                                                                     <  2:297726/94‑1 (MQ=255)                                                             aTTGACGACGCGCTGAAGCTGGCAAATGAAGGTAAAGTAAAAGAAGCGCAGGCTGCTGCAGAGCAACTGAAAGCGACCCGCAATTCATATCACAAGAAGTATCGTTAATTCCTCATTTCCCTGTTGCCTGCACTCAGGt                                                                       <  2:625839/139‑1 (MQ=255)                                                                 acgacgCGCTGAAGCTGGCAAATGAAGGTAAAGTAAAAGAAGCGCAGGCTGCTGCAGAGCAACTGAAAGCGACCCGCAATTCATATCACAAGAAGTATCGTTAATTCCTCATTTCCCTGTTGCCTGCACTCAGGTAACa                                                                   <  2:919460/139‑1 (MQ=255)                                                                   gacgCGCTGAAGCTGGCAAATGAAGGTAAAGTAAAAGAAGCGCAGGCTGCTGCAGAGCAACTGAAAGCGACCCg                                                                                                                                  <  1:1043760/74‑1 (MQ=255)                                                                   gacgCGCTGAAGCTGGCAAATGAAGGTAAAGTAAAAGAAGCGCAGGCTGCTGCAGAGCAACTGAAAGCGACCCg                                                                                                                                  >  2:1043760/1‑74 (MQ=255)                                                                   gacgCGCTGAAGCTGGCAAATGAAGGTAAAGTAAAAGAAGCGCAGGCTGCTGCAGAGCAACTGAAAGCGACCCGCAATTCATATCACAAGAAGTATCGTTAATTCCTCATTTCCCTGTTGCCTGCACTCAGGTAACAgg                                                                 <  2:79498/139‑1 (MQ=255)                                                                              gCTGGCAAATGAAGGTAAAGTAAAAGAAGCGCAGGCTGCTGCAGAGCAACTGAAAGCGACCCGCAATTCATATCACAAGAAGTATCGTTAATTCCTCATTTCCCTGTTGCCTGCACTCAGGTAACAGGGAACTTatcat                                                      <  1:214189/139‑1 (MQ=255)                                                                                 ggCAAATGAAGGTAAAGTAAAAGAAGCGCAGGCTGCTGCAGAGCAACTGAAAGCGACCCGCAATTCATATCACAAGAAGTATCGTTAATTCCTCATTTCCCTGTTGCCTGCACTc                                                                           >  2:9275/1‑115 (MQ=255)                                                                                 ggCAAATGAAGGTAAAGTAAAAGAAGCGCAGGCTGCTGCAGAGCAACTGAAAGCGACCCGCAATTCATATCACAAGAAGTATCGTTAATTCCTCATTTCCCTGTTGCCTGCACTc                                                                           <  1:9275/115‑1 (MQ=255)                                                                                 ggCAAATGAAGGTAAAGTAAAAGAAGCGCAGGCTGCTGCAGAGCAACTGAAAGCGACCCGCAATTCATATCACAAGAAGTATCGTTAATTCCTCATTTCCCTGTTGCCTGCACTCAGGTaa                                                                     <  1:601845/121‑1 (MQ=255)                                                                                 ggCAAATGAAGGTAAAGTAAAAGAAGCGCAGGCTGCTGCAGAGCAACTGAAAGCGACCCGCAATTCATATCACAAGAAGTATCGTTAATTCCTCATTTCCCTGTTGCCTGCACTCAGGTaa                                                                     >  2:601845/1‑121 (MQ=255)                                                                                  gCAAATGAAGGTAAAGTAAAAGAAGCGCAGGCTGCTGCAGAGCAACTGAAAGCGACCCGCAATTCATATCACAAGAAGTATCGTTAATTCCTCATTTCCCTGTTGCCTGCACTCAGGTAACAGGGAACTTATCATCGCa                                                  >  2:267837/1‑139 (MQ=255)                                                                                      aTGAAGGTAAAGTAAAAGAAGCGCAGGCTGCTGCAGAGCAACTGAAAGCGACCCGCAATTCatat                                                                                                                        <  1:299938/65‑1 (MQ=255)                                                                                      aTGAAGGTAAAGTAAAAGAAGCGCAGGCTGCTGCAGAGCAACTGAAAGCGACCCGCAATTCatat                                                                                                                        >  2:299938/1‑65 (MQ=255)                                                                                                agtaaaAGAAGCGCAGGCTGCTGCAGAGCAACTGAAAGCGACCCGCAATTCATATCACAAGAAGTATCGTTAATTCCTCATTTCCCTGTTGCCTGCACTCAGGTAACAGGGAACTTATCATCGc                                                   <  2:1113640/124‑1 (MQ=255)                                                                                                agtaaaAGAAGCGCAGGCTGCTGCAGAGCAACTGAAAGCGACCCGCAATTCATATCACAAGAAGTATCGTTAATTCCTCATTTCCCTGTTGCCTGCACTCAGGTAACAGGGAACTTATCATCGc                                                   >  1:1113640/1‑124 (MQ=255)                                                                                                      agaagCGCAGGCTGCTGCAGAGCAACTGAAAGCGACCCGCAATTCATATCACAAGAAGTATCGTTaa                                                                                                      <  1:62237/67‑1 (MQ=255)                                                                                                      agaagCGCAGGCTGCTGCAGAGCAACTGAAAGCGACCCGCAATTCATATCACAAGAAGTATCGTTaa                                                                                                      >  2:62237/1‑67 (MQ=255)                                                                                                          gcgcAGGCTGCTGCAGAGCAACTGAAAGCGACCCGCAATTCATATCACAAGAAGTATCGTTAATTCCTCATTTCCCTGTTGCCTGCACTCAGGTAACAGGGAACTTATCATCGCAAATGATGCACCACCTGATtgctgc                          >  2:205289/1‑139 (MQ=255)                                                                                                          gcgcAGGCTGCTGCAGAGCAACTGAAAGCGACCCGCAATTCATATCACAAGAAGTATCGTTAATTCCTCATTTCCCTGTTGCCTGCACTCAGGTAACAGGGAACTTATCATCGCAAATGATGCACCACCTGATtgctgc                          >  2:772364/1‑139 (MQ=255)                                                                                                           cgcAGGCTGCTGCAGAGCAACTGAAAGCGACCCGCAATTCATATCACAAGAAGTATCGTTAATTCCTCATTTCCCTGTTGCCTGCACTc                                                                           <  1:837866/89‑1 (MQ=255)                                                                                                           cgcAGGCTGCTGCAGAGCAACTGAAAGCGACCCGCAATTCATATCACAAGAAGTATCGTTAATTCCTCATTTCCCTGTTGCCTGCACTc                                                                           >  2:837866/1‑89 (MQ=255)                                                                                                            gcAGGCTGCTGCAGAGCAACTGAAAGCGACCCGCAATTCat                                                                                                                          <  2:39309/41‑1 (MQ=255)                                                                                                            gcAGGCTGCTGCAGAGCAACTGAAAGCGACCCGCAATTCat                                                                                                                          >  1:39309/1‑41 (MQ=255)                                                                                                            gcAGGCTGCTGCAGAGCAACTGAAAGCGACCCGCAATTCATATCACAAGAAGTATCGTTAATTCCTCATTTCCCTGTTGCCTGCACTCAGGTAACAGGGAACTTatca                                                       <  1:778474/108‑1 (MQ=255)                                                                                                            gcAGGCTGCTGCAGAGCAACTGAAAGCGACCCGCAATTCATATCACAAGAAGTATCGTTAATTCCTCATTTCCCTCTTGCCTGCACTCAGGTACCAGGGAACTTatca                                                       >  2:778474/1‑108 (MQ=255)                                                                                                                          agCAACTGAAAGCGACCCGCAATTCATATCACAAGAAGTATCGTTAATTCCTCAtt                                                                                             <  1:1204004/56‑1 (MQ=255)                                                                                                                          agCAACTGAAAGCGACCCGCAATTCATATCACAAGAAGTATCGTTAATTCCTCAtt                                                                                             >  2:1204004/1‑56 (MQ=255)                                                                                                                              aCTGAAAGCGACCCGCAATTCATATCACAAGAAGTATCGTTAATTCCTCATTTCCCTGTTGCCTGc                                                                               <  1:634425/66‑1 (MQ=255)                                                                                                                              aCTGAAAGCGACCCGCAATTCATATCACAAGAAGTATCGTTAATTCCTCATTTCCCTGTTGCCTGc                                                                               >  2:634425/1‑66 (MQ=255)                                                                                                                                  aaagCGACCCGCAATTCATATCACAAGAAGTATCGTTAATTCCTCATTTCCCTGTTGCCTGCACTCAGGTAACAGGGAACTTATCATCGCAATTGATGCACCACCTGATTGCTGCTGCCGCGCCAGATCAGGGAAGGGt  <  2:1098111/139‑1 (MQ=255)                                                                                                                                  aaagCGACCCGCAATTCATATCACAAGAAGTATCGTTAATTCCTCATTTCCCTGTTGCCTGCACTCAGGTAACAGGGAACTTATCATCGCAAATGATGCACCACCTGATTGCTGCTGCCGCGCCAGATCAGGGAAGGGt  <  2:631185/139‑1 (MQ=255)                                                                                                                                  aaagCGACCCGCAATTCATATCACAAGAAGTATCGTTAATTCCTCATTTCCCTGTTGCCTGCACTCAGGTAACAGGGAACTTATCATCGCAAATGATGCACCACCTGATTGCTGCTGCCGCGCCAGATCAGGGAAGGGt  <  1:924803/139‑1 (MQ=255)                                                                                                                                     |                                                                                                                                        TCACCGGACAGCCCGGAAATGAAAGATTTCCGCCACGGTTTCGACATTCTGGTCGGTCAGATTGACGACGCGCTGAAGCTGGCAAATGAAGGTAAAGTAAAAGAAGCGCAGGCTGCTGCAGAGCAACTGAAAACGACCCGCAATTCATATCACAAGAAGTATCGTTAATTCCTCATTTCCCTGTTGCCTGCACTCAGGTAACAGGGAACTTATCATCGCAAATGATGCACCACCTGATTGCTGCTGCCGCGCCAGATCAGGGAAGGGT  >  NC_000913/4459688‑4459955

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑