Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,489,696 A→G L123P (CTG→CCG)  yjgR ← DUF853 family protein with NTPase fold

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,489,6960AG100.0% 191.8 / NA 52L123P (CTG→CCG) yjgRDUF853 family protein with NTPase fold
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (33/19);  total (33/19)

GAAGGATTTGGCGTTATCGCCGATGTACTGGGTAATTGCCCGCAGATCTTTAAAGTCGAGCAGTAACAGCCCCTGATCGTCAGCAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCACACCACCACCGGATTGGCATGCGGTTGCCAGTCATTGACGCCGATATTTTTAAGCCTT  >  NC_000913/4489560‑4489830
                                                                                                                                        |                                                                                                                                      
gAAGGATTTGGCGTTATCGCCGATGTACTGGGTAATTGCCCGCAGATCTTTAAAGTCGAGCAGTAACAGCCCCTGATCGTCAGCAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACGGt                                                                                                                                      >  2:1043856/1‑139 (MQ=255)
 aaGGATTTGGCGTTATCGCCGATGTACTGGGTAATTGCCCGCAGATCTTTAAAGTCGAGCAGTAACAGCCCCTGATCGTCAGCAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACGGTc                                                                                                                                     >  2:715341/1‑139 (MQ=255)
    gATTTGGCGTTATCGCCGATGTACTGGGTAATTGCCCGCAGATCTTTAAAGTCGAGCAGTAACAGCCCCTGATCGTCAGCAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACGGTCGTg                                                                                                                                  >  2:621230/1‑139 (MQ=255)
      tttGGCGTTATCGCCGATGTACTGGGTAATTGCCCGCAGATCTTTAAAGTCGAGCAGTAACAGCCCCTGATCGTCAGCAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACGGTCGTGcc                                                                                                                                >  2:304102/1‑139 (MQ=255)
               aTCGCCGATGTACTGGGTAATTGCCCGCAGATCTTTAAAGTCGAGCAGTAACAGCCCCTGATCGTCAGCAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACGGTc                                                                                                                                     >  2:882998/1‑125 (MQ=255)
               aTCGCCGATGTACTGGGTAATTGCCCGCAGATCTTTAAAGTCGAGCAGTAACAGCCCCTGATCGTCAGCAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACGGTc                                                                                                                                     <  1:882998/125‑1 (MQ=255)
               aTCGCCGATGTACTGGGTAATTGCCCGCAGATCTTTAAAGTCGAGCAGTAACAGCCCCTGATCGTCAGCAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACGGTCGTGCCAGCAACAGc                                                                                                                       >  1:575051/1‑139 (MQ=255)
                       tGTACTGGGTAATTGCCCGCAGATCTTTAAAGTCGAGCAGTAACAGCCCCTGATCGTCAGCAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACGGTCGTGCCAGCAACAGCGGCCCCAg                                                                                                               >  2:475951/1‑139 (MQ=255)
                                tAATTGCCCGCAGATCTTTAAAGTCGAGCAGTAACAGCCCCTGATCGTCAGCAATGCGGAAGATGATATTAAGCACGCCATATTGCACATCGTTGAGATTAAACGGTCGTGCCCGCAACAGCGGCCCCAGATCCGAAAc                                                                                                      >  2:844273/1‑139 (MQ=255)
                                   ttGCCCGCAGATCTTTAAAGTCGAGCAGTAACAGCCCCTGATCGTCAGCAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACGGTCGt                                                                                                                                   >  1:1047976/1‑107 (MQ=255)
                                   ttGCCCGCAGATCTTTAAAGTCGAGCAGTAACAGCCCCTGATCGTCAGCAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACGGTCGt                                                                                                                                   <  2:1047976/107‑1 (MQ=255)
                                   ttGCCCGCAGATCTTTAAAGTCGAGCAGTAACAGCCCCTGATCGTCAGCAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACGGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGt                                                                                                   >  1:635329/1‑139 (MQ=255)
                                   ttGCCCGCAGATCTTTAAAGTCGAGCAGTAACAGCCCCTGATCGTCAGCAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACGGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGt                                                                                                   >  1:449250/1‑139 (MQ=255)
                                      cccGCAGATCTTTAAAGTCGAGCAGTAACAGCCCCTGATCGTCAGCAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACGGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGc                                                                                                >  1:1189922/1‑139 (MQ=255)
                                        cGCAGATCTTTAAAGTCGAGCAGTAACAGCCCCTGATCGTCAGCAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACGGTCGTGCCAGCAACa                                                                                                                         <  2:569903/112‑1 (MQ=255)
                                        cGCAGATCTTTAAAGTCGAGCAGTAACAGCCCCTGATCGTCAGCAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACGGTCGTGCCAGCAACa                                                                                                                         >  1:569903/1‑112 (MQ=255)
                                        cGCAGATCTTTAAAGTCGAGCAGTAACAGCCCCTGATCGTCAGCAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACGGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGccc                                                                                              >  2:81555/1‑139 (MQ=255)
                                        cGCAGATCTTTAAAGTCGAGCAGTAACAGCCCCTGATCGTCAGCAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACGGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGccc                                                                                              >  1:872932/1‑139 (MQ=255)
                                        cGCAGATCTTTAAAGTCGAGCAGTAACAGCCCCTGATCGTCAGCAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACGGTCGTACCAGCAACAGCGGCCCCAGATCCGAAACCGTCGccc                                                                                              >  1:844791/1‑139 (MQ=255)
                                         gCAGATCTTTAAAGTCGAGCAGTAACAGCCCCTGATCGTCAGCAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACGGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCg                                                                                             <  1:715341/139‑1 (MQ=255)
                                           aGATCTTTAAAGTCGAGCAGTAACAGCCCCTGATCGTCAGCAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACGGTCGTACCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCa                                                                                           <  2:844791/139‑1 (MQ=255)
                                                tttAAAGTCGAGCAGTAACAGCCCCTGATCGTCAGCAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACGGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGa                                                                                      >  1:538599/1‑139 (MQ=255)
                                                         gAGCAGTAACAGCCCCTGATCGTCAGCAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACGGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTtc                                                                             >  2:752170/1‑139 (MQ=255)
                                                         gAGCAGTAACAGCCCCTGATCGTCAGCAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACGGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTtc                                                                             >  2:514984/1‑139 (MQ=255)
                                                         gAGCAGTAACAGCCCCTGATCGTCAGCAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACGGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTtc                                                                             >  2:1139059/1‑139 (MQ=255)
                                                          aGCAGTAACAGCCCCTGATCGTCAGCAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACGGTCGTGCCAGCAACAg                                                                                                                        <  2:980031/95‑1 (MQ=255)
                                                          aGCAGTAACAGCCCCTGATCGTCAGCAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACGGTCGTGCCAGCAACAg                                                                                                                        >  1:980031/1‑95 (MQ=255)
                                                          aGCAGTAACAGCCCCTGATCGTCAGCAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACGGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTtct                                                                            >  1:918518/1‑139 (MQ=255)
                                                             aGTAATAGCCCCTGATCGTCAGCAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATGCAACGGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGc                                                                         <  1:752170/139‑1 (MQ=255)
                                                                  cAGCCCCTGATCGTCAGCAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACGGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAg                                                                    <  1:621230/139‑1 (MQ=255)
                                                                   aGCCCCTGATCGTCAGCAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACGGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGa                                                                   <  2:1018525/139‑1 (MQ=255)
                                                                      cccTGATCGTCAGCAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACGGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGatat                                                                >  1:314083/1‑139 (MQ=255)
                                                                         tGATCGTCAGCAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACGGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGc                                                                                            >  1:1138717/1‑108 (MQ=255)
                                                                         tGATCGTCAGCAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACGGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGc                                                                                            <  2:1138717/108‑1 (MQ=255)
                                                                                 aGCAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACGGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCg                                                                                                 >  2:877839/1‑95 (MQ=255)
                                                                                 aGCAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACGGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCg                                                                                                 <  1:877839/95‑1 (MQ=255)
                                                                                      tGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACGGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCACACCACCACCgg                                                >  2:1067123/1‑139 (MQ=255)
                                                                                      tGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACGGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCACACCACCACCgg                                                >  2:47452/1‑139 (MQ=255)
                                                                                       gCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACGGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCACACCACCACCGGa                                               <  2:918518/139‑1 (MQ=255)
                                                                                                       aGCACGCCAGATTGCACATCGTTGAGATTCAACGGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGc                                                                         <  2:296207/97‑1 (MQ=255)
                                                                                                       aGCACGCCAGATTGCACATCGTTGAGATTCAACGGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGc                                                                         >  1:296207/1‑97 (MQ=255)
                                                                                                        gCACGCCAGATTGCACATCGTTGAGATTCAACGGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCACACCACCACCGGATTGGCATGCGGTTGCCa                              >  1:721014/1‑139 (MQ=255)
                                                                                                                gATTGCACATCGTTGAGATTCAACGGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCACACCACCACCGGATTGGCATGCGGTTGCCAGTCATTGa                      <  2:635329/139‑1 (MQ=255)
                                                                                                                  ttGCACATCGTTGAGATTCAACGGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCACACCACCACCGGATTGGCATGCGGTTGCCAGTCATTGACg                    <  1:81555/139‑1 (MQ=255)
                                                                                                                      acaTCGTTGAGATTCAACGGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCACACCACCACCGGATTGGCATGCGGtt                                  <  2:779435/121‑1 (MQ=255)
                                                                                                                      acaTCGTTGAGATTCAACGGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCACACCACCACCGGATTGGCATGCGGtt                                  >  1:779435/1‑121 (MQ=255)
                                                                                                                           gTTGAGATTCAACGGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCACACCACCACCg                                                 >  1:579966/1‑101 (MQ=255)
                                                                                                                           gTTGAGATTCAACGGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCACACCACCACCg                                                 <  2:579966/101‑1 (MQ=255)
                                                                                                                           gTTGAGATTCAACGGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCACACCACCACCGGATTGGCATGCGGTTGCCAGTCATTGACGCCGATAttt           >  1:282192/1‑139 (MQ=255)
                                                                                                                             tGAGATTCAACGGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCACACCACCACCGGATTGGCATGCGGTTGCCAGTCATTGACGCCGATAttttt         >  1:67039/1‑139 (MQ=255)
                                                                                                                               agaTTCAACGGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCACACCACCACCGGATTGGCATGCGGTTGCCAGTCATTGACGCCGATATTTTTaa       <  2:538599/139‑1 (MQ=255)
                                                                                                                                    cAACGGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCACACCACCACCGGATTGGCATGCGGTTGCCAGTCATTGACGCCGATATTTTTAAGCCtt  <  2:872932/139‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                      
GAAGGATTTGGCGTTATCGCCGATGTACTGGGTAATTGCCCGCAGATCTTTAAAGTCGAGCAGTAACAGCCCCTGATCGTCAGCAATGCGGAAGATGATATTCAGCACGCCAGATTGCACATCGTTGAGATTCAACAGTCGTGCCAGCAACAGCGGCCCCAGATCCGAAACCGTCGCCCGCACCGGATGGCCTTTCTCGCCAAAGATATCCCACACCACCACCGGATTGGCATGCGGTTGCCAGTCATTGACGCCGATATTTTTAAGCCTT  >  NC_000913/4489560‑4489830

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑