Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,574,259	A→G	pseudogene (341/2937 nt)	yjiV →	pseudogene, conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,574,259	0	A	G	100.0%	180.7 / NA	49	pseudogene (341/2937 nt)	yjiV	pseudogene, conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/21);  total (28/21)

TTGGCTATGTGTATGTGAACCAGCGACAAAATTGTGACTGTGGAAGCCCTGTATACGAAGTTGCATTCTGTAATGATTGTAATGAGCCTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGAAAATAAAGGTGGCGATGAATTCTCTTTGCAGGATGAAGTACCTGTTGAACATGACGCTACAGAAGAAAAAGTCGAAAAAGAGAACAGTTTTCAGCCTCCGTTGATTATTGCCGCAGGAGAGACCAGCGAGGCAGG  >  NC_000913/4574127‑4574394                                                                                                                                     |                                                                                                                                        ttGGCTATGTGTATGTGAACCAGCGACAAAATTGTGACTGTGGAAGCCCTGTATACGAAGTTGCATTCTGTAATGATTGTAATGAGCCTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATaa                                                                                                                                   >  2:949120/1‑139 (MQ=255)        tgtgtatgtgAACCAGCGACAAAATTGTGACTGTGGAAGCCCTGTATACGAAGTTGCATTCTGTAATGATTGTAATGAGCCTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGc                                                                                                                            <  1:690734/139‑1 (MQ=255)        tgtgtatgtgAACCAGCGACAAAATTGTGACTGTGGAAGCCCTGTATACGAAGTTGCATTCTGTAATGATTGTAATGAGCCTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGc                                                                                                                            <  1:949120/139‑1 (MQ=255)        tgtgtatgtgAACCAGCGACAAAATTGTGACTGTGGAAGCCCTGTATACGAAGTTGCATTCTGTAATGATGGTAATGAGCCTCATCTTCTGGCACGTGTCAAAAAGGTCAAACTAGTCCAGTGGGGAAATAAAGGTGGc                                                                                                                            >  1:1068234/1‑139 (MQ=255)             atgtgAACCAGCGACAAAATTGTGACTGTGGAAGCCCTGTATACGAAGTTGCATTCTGTAATGATTGTAATGAGCCTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGa                                                                                                                       >  1:249703/1‑139 (MQ=255)             atgtgAACCAGCGACAAAATTGTGACTGTGGAAGCCCTGTATACGAAGTTGCATTCTGTAATGATTGTAATGAGCCTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGa                                                                                                                       <  1:666469/139‑1 (MQ=255)                  aaCCAGCGACAAAATTGTGACTGTGGAAGCCCTGTATACGAAGTTGCATTCTGTAATGATTGTAATGAGCCTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGAATtct                                                                                                                  >  2:447411/1‑139 (MQ=255)                   aCCAGCGACAAAATTGTGACTGTGGAAGCCCTGTATACGAAGTTGCATTCTGTAATGATTGTAATGAGCCTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGAATtctc                                                                                                                 >  1:245170/1‑139 (MQ=255)                   aCCAGCGACAAAATTGTGACTGTGGAAGCCCTGTATACGAAGTTGCATTCTGTAATGATTGTAATGAGCCTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGAATtctc                                                                                                                 >  2:922933/1‑139 (MQ=255)                    ccAGCGACAAAATTGTGACTGTGGAAGCCCTGTATACGAAGTTGCATTCTGTAATGATTGTAATGAGCCTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGAATTctct                                                                                                                >  2:348803/1‑139 (MQ=255)                    ccAGCGACAAAATTGTGACTGTGGAAGCCCTGTATACGAAGTTGCATTCTGTAATGATTGTAATGAGCCTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGAATTctct                                                                                                                >  2:538/1‑139 (MQ=255)                       gCGACAAAATTGTGACTGTGGAAGCCCTGTATACGAAGTTGCATTCTGTAATGATTGTAATGAGCCTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGAATTCTCTTTg                                                                                                             <  1:373454/139‑1 (MQ=255)                         gACAAAATTGTGACTGTGGAAGCCCTGTATACGAAGTTGCATTCTGTAATGATTGTAATGAGCCTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGAATTCTCTTTGCa                                                                                                           <  1:233362/139‑1 (MQ=255)                         gACAAAATTGTGACTGTGGAAGCCCTGTATACGAAGTTGCATTCTGTAATGATTGTAATGAGCCTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGAATTCTCTTTGCa                                                                                                           <  1:538/139‑1 (MQ=255)                            aaaaTTGTGACTGTGGAAGCCCTGTATACGAAGTTGCATTCTGTAATGATTGTAATGAGCCTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGAATTCTCTTTGCAGGa                                                                                                        <  1:447411/139‑1 (MQ=255)                                       tgtgGAAGCCCTGTATACGAAGTTGAATTCTGTAATGATTGTAATGAGCCTCATCTTCTGGCAAGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGAATTCTCTTTGCAGGATGAAGTACCTg                                                                                             <  1:635474/139‑1 (MQ=255)                                         tgGAAGCCCTGTATACGAAGTTGCATTCTGTAATGATTGTAATGAGCCTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGAATTCTCTTTGCAGGATGAAGTAc                                                                                                <  2:219224/134‑1 (MQ=255)                                         tgGAAGCCCTGTATACGAAGTTGCATTCTGTAATGATTGTAATGAGCCTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGAATTCTCTTTGCAGGATGAAGTAc                                                                                                >  1:219224/1‑134 (MQ=255)                                                   gTATACGAAGTTGCATTCTGTAATGATTGTAATGAGCCTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGAATTCTCTTTGCAGGATGAAGTACCTGTTGAACATGACg                                                                                 <  2:272126/139‑1 (MQ=255)                                                     ataCGAAGTTGCATTCTGTAATGATTGTAATGAGCCTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGAATTCTCTTTGCAGGATGAAGTACCTGTTGAACATGACGCt                                                                               >  2:909467/1‑139 (MQ=255)                                                              tGCATTCTGTAATGATTGTAATGAGCCTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGAAt                                                                                                                     <  1:899817/92‑1 (MQ=255)                                                              tGCATTCTGTAATGATTGTAATGAGCCTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGAAt                                                                                                                     >  2:899817/1‑92 (MQ=255)                                                              tGCATTCTGTAATGATTGTAATGAGCCTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGAATTCTCTTTGCAGGATGAAGTACCTGTTGAACATGACGCTACAgaagaa                                                                      >  2:867739/1‑139 (MQ=255)                                                              tGCATTCTGTAATGATTGTAATGAGCCTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGAATTCTCTTTGCAGGATGAAGTACCTGTTGAACATGACGCTACAgaagaa                                                                      >  1:11050/1‑139 (MQ=255)                                                              tGCATTCTGTAATGATTGTAATGAGCCTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGAATTCTCTTTGCAGGATGAAGTACCTGTTGAACATGACGCTACAgaagaa                                                                      >  1:504811/1‑139 (MQ=255)                                                              tGCATTCTGTAATGATTGTAATGAGCCTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGAATTCTCTTTGCAGGATGAAGTACCTGTTGAACATGACGCTACAgaagaa                                                                      >  1:699272/1‑139 (MQ=255)                                                                       tAATGATTGTAATGAGCCTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGAATTCTCTTTGCAGGATGAAGTACCTGTTGAACATGACGCTACAGAAGAAAAAGTCGaa                                                             >  2:759527/1‑139 (MQ=255)                                                                               gTAATGAGCCTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAGGGTGGCGATGAATTCTCTTTGCAGGATGAAGTACCTGTTGAACATGACGCTACAGAAGAAAAAGTCGAAAAAGAGaa                                                     >  1:829912/1‑139 (MQ=255)                                                                               gTAATGAGCCTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGAATTCTCTTTGCAGGATGAAGTACCTGTTGAACATGa                                                                                   <  1:419775/109‑1 (MQ=255)                                                                               gTAATGAGCCTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGAATTCTCTTTGCAGGATGAAGTACCTGTTGAACATGa                                                                                   >  2:419775/1‑109 (MQ=255)                                                                                 aaTGAGCCTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGAATTCTCTTTGCAGGATGAAGTACCTGTTGAACATGACGCTACAGAAGAAAAAGTCGAAAAAGAGAACa                                                   >  2:556845/1‑139 (MQ=255)                                                                                 aaTGAGCCTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGAATTCTCTTTGCAGGATGAAGTACCTGTTGAACATGACGCTACAGAAGAAAAAGTCGAAAAAGAGAACa                                                   >  1:868473/1‑139 (MQ=255)                                                                                 aaTGAGCCTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGAATTCTCATTGCAGGATGAAGTACCTGTTGAACATGACGCTACAGAAGAAAAAGTCGAAAAAGAGAACa                                                   >  2:765718/1‑139 (MQ=255)                                                                                     aGCCTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAAt                                                                                                                                     >  1:403855/1‑53 (MQ=255)                                                                                     aGCCTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAAt                                                                                                                                     <  2:403855/53‑1 (MQ=255)                                                                                     aGCCTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGAATTCTCTTTGCAGGATGAAGTACCTGTTGAACATGACGCTACAGAAGAAAAAGTCGAAAAAGAGAACAGttt                                               >  1:28213/1‑139 (MQ=255)                                                                                     aGCCTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGAATTCTCTTTGCAGGATGAAGTACCTGTTGAACATGACGCTACAGAAGAAAAAGTCGAAAAAGAGAACAGttt                                               >  2:920814/1‑139 (MQ=255)                                                                                      gCCTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGAATTCTCTTTGCAGGa                                                                                                        >  2:880131/1‑81 (MQ=255)                                                                                      gCCTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGAATTCTCTTTGCAGGa                                                                                                        <  1:880131/81‑1 (MQ=255)                                                                                      gCCTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGAATTCTCTTTGCAGGATGAAGTACCTGTTGAACATGACGCTACAGAAGAAAAAGTCGAAAAAGAGAACAGtttt                                              >  1:910767/1‑139 (MQ=255)                                                                                      gCCTCATCTTCTGGCACGGGACAAAAAGGACAAACTAGTCCAGTGCGGAAATCAAGGTGGCGATGAATTCTCTTTGCAGGATGAAGTACCTGTTGAACATGACGCTACAGAAGAAAAAGTCGAAAAAGAGAACAGtttt                                              >  2:698091/1‑139 (MQ=255)                                                                                          cATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGAATTCTCTTTGCAGGATGAAGTACCTGTTGAACATGACGCTACAGAAGAAAAAGTCGAAAAAGAGAACAGTTTTCAGc                                          <  1:920814/139‑1 (MQ=255)                                                                                                       gggACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGAATTCTCTTTGCAGGATGAAGTACCTGTTGAACATGACGCTACAGAAGAAAAAGTCGAAAAAGAGAACAGTTTTCAGCCTCCGTTGattat                             <  2:868473/139‑1 (MQ=255)                                                                                                         gACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGAATTCTCTTTGTAGGATGAAGTACCTGTTGAACATGACGCTa                                                                              >  1:1125599/1‑88 (MQ=255)                                                                                                         gACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGAATTCTCTTTGCAGGATGAAGTACCTGTTGAACATGACGCTa                                                                              <  2:1125599/88‑1 (MQ=255)                                                                                                         gACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGAATTCTCTTTGCAGGATGAAGTACCTGTTGAACATGACGCTACAGAAGAAAAAGTCGAAAAAGAGAACAGTTTTCAGCCTCCGTTGATTATTg                           <  2:333720/139‑1 (MQ=255)                                                                                                                 gggCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGAATTCTCTTTGCAGGATGAAGTACCTGTTGAACATGACGCTACAGAAGAAAAAGTCGAAAAAGAGAACAGTTTTCAGCCTCCGTTGATTATTGCCGCAGga                   <  2:28213/139‑1 (MQ=255)                                                                                                                         tAGTCCAGTGGGGAAATAAAGGTGGCGATGAATTCTCTTTGCAGGATGAAGTACCTGTTGAACATGACGCTACAGAAGAAAAAGTCGAAAAAGAGAACAGTTTTCAGCCTCCGTTGATTATTGCCGCAGGAGAGACCAg           <  1:765718/139‑1 (MQ=255)                                                                                                                               aGTGGGGAAATAAAGGTGGCGATGAATTCTCTTTGCAGGATGAAGTACCTGTTGAACATGACGCTACAGAAGAAAAAGTCGAAAAAGAGAACAGTTTTCAGCCTCCGTTGATTATTGCCGCAGGAGAGACCAGCGAGGc     <  2:249703/139‑1 (MQ=255)                                                                                                                                  ggggAAATAAAGGTGGCGATGAATTCTCTTTGCAGGATGAAGTACCTGTTGAACATGACGCTACAGAAGAAAAAGTCGAAAAAGAGAACAGTTTTCAGCCTCCGTTGATTATTGCCGCAGGAGAGACCAGCGAGGCAgg  <  2:389046/139‑1 (MQ=255)                                                                                                                                  ggggAAATAAAGGTGGCGATGAATTCTCTTTGCAGGATGAAGTACCTGTTGAACATGACGCTACAGAAGAAAAAGTCGAAAAAGAGAACAGTTTTCAGCCTCCGTTGATTATTGCCGCAGGAGAGACCAGCGAGGCAgg  <  1:456618/139‑1 (MQ=255)                                                                                                                                     |                                                                                                                                        TTGGCTATGTGTATGTGAACCAGCGACAAAATTGTGACTGTGGAAGCCCTGTATACGAAGTTGCATTCTGTAATGATTGTAATGAGCCTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGAAAATAAAGGTGGCGATGAATTCTCTTTGCAGGATGAAGTACCTGTTGAACATGACGCTACAGAAGAAAAAGTCGAAAAAGAGAACAGTTTTCAGCCTCCGTTGATTATTGCCGCAGGAGAGACCAGCGAGGCAGG  >  NC_000913/4574127‑4574394

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑