Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	662,907	G→A	Q353* (CAA→TAA)	dacA ←	D‑alanyl‑D‑alanine carboxypeptidase (penicillin‑binding protein 5)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	662,907	0	G	A	100.0%	151.9 / NA	44	Q353* (CAA→TAA)	dacA	D‑alanyl‑D‑alanine carboxypeptidase (penicillin‑binding protein 5)
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/24);  total (20/24)

ACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCTTGCAACACAACCAGCGGGCGTTGCTCGATCGTTTTGCCATCAAGCTGGAAGTTGATAGTTCCGACGACCTGATTCTTTTGCAGCGGCGCATGCAATTCACTGCTGTTCAGCACATAGCTGGCTTTCAGATCTTTCATGCGACCACGCGGAATGGTCAGGTACACGTCTTTATCAACCCCTAACGAAGCGCGATCAGAATCAC  >  NC_000913/662772‑663029                                                                                                                                        |                                                                                                                           aCATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCAGGATTTCTTGCAACACAACCAGCGGGCGTTGCTCGATCGTTTTGCCATCAAGCTGGAAGTTGATAGTTCCGACGACCTGATTCTTTTACAg                                                                                                                         >  2:1198455/1‑139 (MQ=255)    ttaatttaatGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCTTGCAACACAACCAGCGGGCGTTGCTCGATCGTTTTGCCATCAAGCTGGAAGTTGATAGTTCCGACGACCTGATTCTTTTACAgcgg                                                                                                                      >  2:832455/1‑139 (MQ=255)     taatttaatGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCTTGCAACACAACCAGCGGGCGTTGCTCGATCGTTTTGCCATCAAGCTGGAAGTTGATAGTTCCGACGACCTGATTCTTTTACAgcggc                                                                                                                     >  1:1180871/1‑139 (MQ=255)       atttaatGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCTTGCAACACAACCAGCGGGCGTTGCTCGATCGTTTTGCCATCAAGCTGGAAGTTGATAGTTCCGACGACCTGATTCTTTTACAGCGgcgc                                                                                                                   >  1:943972/1‑139 (MQ=255)             tGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCTTGCAACACAACCAGCGGGCGTTGCTCGATCGTTTTGCCATCAAGCTGGAAGTTGATAGTTCCGACGACCTGATTCTTTTACAGCGGCGCATGCaa                                                                                                             >  2:912429/1‑139 (MQ=255)                 aTCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCTTGCAACACAACCAGCGGGCGTTGCTCGATCGTTTTGCCATCAAGCTGGAAGTTGATAGTTCCGACGACCTGATTCTTTTACAGCGGCGCATg                                                                                                                <  2:537223/132‑1 (MQ=255)                 aTCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCTTGCAACACAACCAGCGGGCGTTGCTCGATCGTTTTGCCATCAAGCTGGAAGTTGATAGTTCCGACGACCTGATTCTTTTACAGCGGCGCATg                                                                                                                >  1:537223/1‑132 (MQ=255)                    aaTGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCTTGCAACACAACCAGCGGGCGTTGCTCGATCGTTTTGCCATCAAGCTGGAAGTTGATAGTTCCGACGACCTGATTCTTTTACAGCGGCGCATGCAATTCActg                                                                                                      <  2:921753/139‑1 (MQ=255)                         ttttGCCGAAGAAGTTACCTTCCGGGATTTCTTGCAACACAACCAGCGGGCGTTGCTCGATCGTTTTGCCATCAAGCTGGAAGTTGATAGTTCCGACGACCTGATTCTTTTACAGCGGCGCATGCAATTCACTGCTGtt                                                                                                 <  2:943972/139‑1 (MQ=255)                            tGCCGAAGAAGTTACCTTCCGGGATTTCTTGCAACACAACCAGCGGGCGTTGCTCGATCGTTTTGCCATCAAGCTGGAAGTTGATAGTTCCGACGACCTGATTCTTTTACAGCGGCGCATGCAATTCCCTGCTGTTCAg                                                                                              >  1:786110/1‑139 (MQ=255)                                                            aaCACAACCAGCGGGCGTTGCTCGATCGTTTTGCCATCAAGCTGGAAGTTGATAGTTCCGACGACCTGATTCTTTTACAGCGGCGCATGCAATTCACTGCTGTTCAGCACATAGCTGGCTTTCAGATCTTTCATGCGAc                                                              >  1:512594/1‑139 (MQ=255)                                                             acacAACCAGCGGGCGTTGCTCGATCGTTTTGCCATCAAGCTGGAAGTTGATAGTTCCGACGACCTGATTCTTTTACAGCGGCGCATGCAATTCACTGCTGTTCAGCACATAGCTGGCTTTCAGATCTTTCATGCGAcc                                                             >  2:494022/1‑139 (MQ=255)                                                              cacaACCAGCGGGCGTTGCTCGATCGTTTTGCCATCAAGCTGGAAGTTGATAGTTCCGACGACCTGATTCTTTTACAGCGGCGCATGCAATTCACTGCTGTTCAGCACATAGCTGGCTTTCAGATCTTTCATGCGACCa                                                            <  2:967939/139‑1 (MQ=255)                                                                     aGCGGGCGTTGCTCGATCGTTTTGCCATCAAGCTGGAAGTTGATAGTTCCGACGACCTGATTCTTTTACAGCGGCGCATGCAATTCACTGCTGTTCAGCACATAGCTGGCTTTCAGATCTTTCATGCGACCACGCGGaa                                                     <  2:649549/139‑1 (MQ=255)                                                                      gCGGGCGTTGCTCGATCGTTTTGCCATCAAGCTGGAAGTTGATAGTTCCGACGACCTGATTCTTTTACAGCGGCGCATGCAATTCACTGCTGTTCAGCACATAGCTGGCTTTCAGATCTTTCATGCGACCACGCGGAAt                                                    >  1:890638/1‑139 (MQ=255)                                                                       cGGGCGTTGCTCGATCGTTTTGCCATCAAGCTGGAAGTTGATAGTTCCGACGACCTGATTCTTTTACAGCGGCGCATGCAATTCACTGCTGTTCAGCACATAGCTGGCTTTCAGATCTTTCATGCGACCACGCGGAATg                                                   >  1:1039643/1‑139 (MQ=255)                                                                        gggCGTTGCTCGATCGTTTTGCCATCAAGCTGGAAGTTGATAGTTCCGACGACCTGATTCTTTTACAGCGGCGCATGCAATTCACTGCTGTTCAGCACATAGCTGGCTTTCAGATCTTTCATGCGACCACGCGGAATgg                                                  >  1:439463/1‑139 (MQ=255)                                                                         ggCGTTGCTCGATCGTTTTGCCATCAAGCTGGAAGTTGATAGTTCCGACGACCTGATTCTTTTACAGCGGCGCATGCAATTCACTGCTGTTCAGCACATAGCTGGCTTTCAGATCTTTCATGCGACCACGCGGAATGGt                                                 <  2:890638/139‑1 (MQ=255)                                                                         ggCGTTGCTCGATCGTTTTGCCATCAAGCTGGAAGTTGATAGTTCCGACGACCTGATTCTTTTACAGCGGCGCATGCAATTCACTGCTGTTCAGCACATAGCTGGCTTTCAGATCTTTCATGCGACCACGCGGAATGGt                                                 >  2:1171754/1‑139 (MQ=255)                                                                          gCGTTGCTCGATCGTTTTGCCATCAAGCTGGAAGTTGATAGTTCCGACGACCTGATTCTTTTACAGCGGCGCATGCAATTCACTGCTGTTCAGCACATAGCTGGCTTTCAGATCTTTCATGCGACCACGCGGAATGGTc                                                <  1:602571/139‑1 (MQ=255)                                                                           cGTTGCTCGATCGTTTTGCCATCAAGCTGGAAGTTGATAGTTCCGACGACCTGATTCTTTTACAGCGGCGCATg                                                                                                                >  1:897435/1‑74 (MQ=255)                                                                           cGTTGCTCGATCGTTTTGCCATCAAGCTGGAAGTTGATAGTTCCGACGACCTGATTCTTTTACAGCGGCGCATg                                                                                                                <  2:897435/74‑1 (MQ=255)                                                                            gTTGCTCGATCGTTTTGCCATCAAGCTGGAAGTTGATAGTTCCGACGACCTGATTCTTTTACAGCGGCGCATGCAATTCACTGCTGTTCAGCACATAGCTGGCTTTCAGATCTTTCATGCGACCACGCGGAATGGTCAg                                              <  2:17426/139‑1 (MQ=255)                                                                                 tCGATCGTTTTGCCATCAAGCTGGAAGTTGATAGTTCCGACGACCTGATTCTTTTACAGCGGCGCATGCAATTCACTGCTGTTCAGCACATAGCTGGCTTTCAGATCTTTCATGCGACCACGCGGAATGGTCAGGTaca                                         <  2:439463/139‑1 (MQ=255)                                                                                    aTCGTTTTGCCATCAAGCTGGAAGTTGATAGTTCCGACGACCTGATTCTTTTACAGCGGCGCATGCAATTCACTGCTGTTCAGCACATAGCTGGCTTTCGGATCTTTCATGCGACCACGCGGAATGGTCAGGTACACGt                                      <  1:85274/139‑1 (MQ=255)                                                                                    aTCGTTTTGCCATCAAGCTGGAAGTTGATAGTTCCGACGACCTGATTCTTTTACAGCGGCGCATGCAATTCACTGCTGTTCAGCACATAGCTGGCTTTCAGATCTTTCATGCGACCACGCGGAATGGTCAGGTACACGt                                      <  2:786110/139‑1 (MQ=255)                                                                                    aTCGTTTTGCCATCAAGCTGGAAGTTGATAGTTCCGACGACCTGATTCTTTTACAGCGGCGCATGCAATTCACTGCTGTTCAGCACATAGCTGGCTTTCAGATCTTTCATGCGACCACGCGGAATGGTCAGGTACACGt                                      >  2:977494/1‑139 (MQ=255)                                                                                    aTCGTTTTGCCATCAAGCTGGAAGTTGATAGTTCCGACGACCTGATTCTTTTACAGCGGCGCATGCAATTCACTGCTGTTCAGCACATAGCTGGCTTTCAGATCTTTCATGCGACCACGCGGAATGGTCAGGTACACGt                                      >  2:107635/1‑139 (MQ=255)                                                                                    aTCGTTTTGCCATCAAGCTGGAAGTTGATAGTTCCGACGACCTGATTCTTTTACAGCGGCGCATGCAATTCACTGCTGTTCAGCACATAGCTGGCTTTCAGATCTTTCATGCGACCACGCGGAATGGTCAGGTACACGt                                      <  1:494022/139‑1 (MQ=255)                                                                                      cGTTTTGCCATCAAGCTGGAAGTTGATAGTTCCGACGACCTGATTCTTTTACAGCGGCGCATGCAATTCACTGCTGTTCAGCACATAGCTGGCTTTCAGATCTTTCATGCGACCACGCGGAATGGTCAGGTACACGTCt                                    <  2:1013816/139‑1 (MQ=255)                                                                                        ttttGCCATCAAGCTGGAAGTTGATAGTTCCGACGACCTGATTCTTTTACAGCGGCGCATGCAATTCa                                                                                                         >  1:414786/1‑68 (MQ=255)                                                                                        ttttGCCATCAAGCTGGAAGTTGATAGTTCCGACGACCTGATTCTTTTACAGCGGCGCATGCAATTCa                                                                                                         <  2:414786/68‑1 (MQ=255)                                                                                        ttttGCCATCAAGCTGGAAGTTGATAGTTCCGACGACCTGATTCTTTTACAGCGGCGCATGCAATTCACTGCTGTTc                                                                                                <  1:1126831/77‑1 (MQ=255)                                                                                        ttttGCCATCAAGCTGGAAGTTGATAGTTCCGACGACCTGATTCTTTTACAGCGGCGCATGCAATTCACTGCTGTTc                                                                                                >  2:1126831/1‑77 (MQ=255)                                                                                          ttGCCATCAAGCTGGAAGTTGATAGTTCCGACGACCTGATTCTTTTACAGCGGCGCATGCAATTCACTGCTGTTCAGCACATAGCTGGCTTTCAGATCTTTCATGCGACCACGCGGAATGGTCAGGTACACGTCTTTAt                                <  2:1180871/139‑1 (MQ=255)                                                                                            gCCATCAAGCTGGAAGTTGATAGTTCCGACGACCTGATTCTTTTACAGCGGCGCATGCAATTCACTGCTGTTCAGCACATAGCTGGCTTTCAGATCTTTCATGCGACCACGCGGAATGGTCAGGTACACGTCTTTATCa                              <  2:1039643/139‑1 (MQ=255)                                                                                                          aGTTGATAGTTCCGACGACCTGATTCTTTTACAGCGGCGCATGCAATTCACTGCTGTTCAGCACATAGCTGGCTTTCAGATCTTTCATGCGACCACGCGGAATGGTCAGGTACACGTCTTTATCa                              <  2:862327/125‑1 (MQ=255)                                                                                                          aGTTGATAGTTCCGACGACCTGATTCTTTTACAGCGGCGCATGCAATTCACTGCTGTTCAGCACATAGCTGGCTTTCAGATCTTTCATGCGACCACGCGGAATGGTCAGGTACACGTCTTTATCa                              >  1:862327/1‑125 (MQ=255)                                                                                                          aGTTGATAGTTCCGACGACCTGATTCTTTTACAGCGGCGCATGCAATTCACTGCTGTTCAGCACATAGCTGGCTTTCAGATCTTTCATGCGACCACGCGGAATGGTCAGGTACACGTCTTTATCAACCCCTAACGAAgc                <  1:1171754/139‑1 (MQ=255)                                                                                                           gTTGATAGTTCCGACGACCTGATTCTTTTACAGCGGCGCATGCAATTCACTGCTGTTCAGCACATAGCTGGCTTTCAGATCTTTCATGCGACCACGCGGAATGGCCAGGTACACGTCTTTATCAACCCCTAACGAAgcg               <  1:912429/139‑1 (MQ=255)                                                                                                             tGATAGTTCCGACGACCTGATTCTTTTACAGCGGCGCATGCAATTCACTGCTGTTCAGCACATAGCTGGCTTTCAGATCTTTCATGCGACCACGCGGAATGGTCAGGTACACGTCTTTATCAACCCCTAACGAAGcgcg             <  1:832455/139‑1 (MQ=255)                                                                                                               aTAGTTCCGACGACCTGATTCTTTTACAGCGGCGCATGCAATTCACTGCTGTTCAGCACATAGCTGGCTTTCAGATCTTTCATGCGACCACGCGGAATGGTCAGGTACACGTCTTTATCAACCCCTAACGAAGCGCGAt           >  1:363801/1‑139 (MQ=255)                                                                                                                        acgacCTGATTCTTTTACAGCGGCGCATGCAATTCACTGCTGTTCAGCACATAGCTGGCTTTCAGATCTTTCATGCGACCACGCGGaa                                                     <  2:53270/88‑1 (MQ=255)                                                                                                                        acgacCTGATTCTTTTACAGCGGCGCATGCAATTCACTGCTGTTCAGCACATAGCTGGCTTTCAGATCTTTCATGCGACCACGCGGaa                                                     >  1:53270/1‑88 (MQ=255)                                                                                                                        acgacCTGATTCTTTTACAGCGGCGCATGCAATTCACTGCTGTTCAGCACATAGCTGGCTTTCAGATCTTTCATGCGACCACGCGGAATGGTCAGGTACACGTCTTTATCAACCCCTAACGAAGCGCGATCAGAATCac  <  1:977494/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                           ACATTAATTTAATGTAATCAATGATTTTGCCGAAGAAGTTACCTTCCGGGATTTCTTGCAACACAACCAGCGGGCGTTGCTCGATCGTTTTGCCATCAAGCTGGAAGTTGATAGTTCCGACGACCTGATTCTTTTGCAGCGGCGCATGCAATTCACTGCTGTTCAGCACATAGCTGGCTTTCAGATCTTTCATGCGACCACGCGGAATGGTCAGGTACACGTCTTTATCAACCCCTAACGAAGCGCGATCAGAATCAC  >  NC_000913/662772‑663029

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑