Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,756,216	(A)5→4	coding (519/1413 nt)	pykF →	pyruvate kinase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,756,212	0	A	.	100.0%	93.5 / NA	22	coding (515/1413 nt)	pykF	pyruvate kinase I
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (11/11);  total (11/11)

CGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCC  >  NC_000913/1756075‑1756343                                                                                                                                          |                                                                                                                                    cGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGaa                                                                                                                                    >  1:213702/1‑139 (MQ=255)               gTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAGGAc                                                                                                                     <  2:310216/139‑1 (MQ=255)                tATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAGGAcc                                                                                                                    >  2:172809/1‑139 (MQ=255)                          aCCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTCTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAGGACCTGATCTTTgg                                                                                                          >  1:421149/1‑139 (MQ=255)                          aCCGCCATTGAAGGAAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGCACCTGCCTGGCGTTTCCATTGCTCTGACAGCACTGGCTG‑AAAAGACAAACAGGACCTGATCTTTgg                                                                                                          >  2:312340/1‑139 (MQ=255)                            cGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAGGACCTGATCTTTGGtt                                                                                                        <  1:392028/139‑1 (MQ=255)                              ccATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAGGAcc                                                                                                                    <  2:471405/125‑1 (MQ=255)                              ccATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAGGAcc                                                                                                                    >  1:471405/1‑125 (MQ=255)                                  tGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAGGACCTGATCTTTGGTTGCGAAc                                                                                                  <  2:460312/139‑1 (MQ=255)                                         aaCAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGATAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGt                                                                                           >  2:309955/1‑139 (MQ=255)                                           cAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAg                                                                                         >  1:148300/1‑139 (MQ=255)                                                  aTCTATAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTttgt                                                                                  <  2:86353/139‑1 (MQ=255)                                                    cTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTttgttg                                                                                <  1:427151/139‑1 (MQ=255)                                                      gTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTtgct                                                                              <  2:213702/139‑1 (MQ=255)                                                          aGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCtt                                                                          >  1:108236/1‑139 (MQ=255)                                                                                ggCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGa                                                    >  2:344793/1‑139 (MQ=255)                                                                                ggCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGa                                                    >  2:73495/1‑139 (MQ=255)                                                                                 gCGAAAACTAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGAc                                                   <  1:312340/139‑1 (MQ=255)                                                                                  cGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACg                                                  <  1:344793/139‑1 (MQ=255)                                                                                            ggTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAAt                                        <  1:421753/139‑1 (MQ=255)                                                                                                          tgGCGTTTCCATTGCTCTGCCAGCACTGGCTG‑AAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGa                          >  1:394871/1‑139 (MQ=255)                                                                                                                            gCCAGCACTGGCTG‑AAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGaaa        <  2:394871/139‑1 (MQ=255)                                                                                                                                  aCTGGCTG‑AAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATcc  >  2:327408/1‑139 (MQ=255)                                                                                                                                          |                                                                                                                                    CGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCC  >  NC_000913/1756075‑1756343

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑