Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,968,260 (T)6→5 coding (175/2247 nt) ptsP ← PEP‑protein phosphotransferase enzyme I, GAF domain containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,968,2550T.100.0% 116.2 / NA 27coding (180/2247 nt)ptsPPEP‑protein phosphotransferase enzyme I, GAF domain containing protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (12/15);  total (12/15)

CGGAGGGGATGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGGTTTTTTCAGCCCCCGGGTCGCCATCAGGTAGTAACAACGTCGATCATGATCGGCCAGGTAGACCGAACAGACCTCGGTATCCATCGCAAGACAGATGTCGGTAACCAGAATATTTAACGCCTCATTCAGGCGTGG  >  NC_000913/2968131‑2968389
                                                                                                                            |                                                                                                                                      
cGGAGGGGATGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGGTTTTTCAGCCCCCgg                                                                                                                         >  2:379588/1‑139 (MQ=255)
  gAGGGGATGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGGTTTTTCAGCCCCCGGGt                                                                                                                       >  1:158808/1‑139 (MQ=255)
  gAGGGGATGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGGTTTTTCAGCCCCCGGGt                                                                                                                       <  1:63573/139‑1 (MQ=255)
    ggggATGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGGTTTTTCAGCCCCCGGGTCg                                                                                                                     <  1:100336/139‑1 (MQ=255)
     gggATGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGGTTTTTCAGCCCCCGGGTCGc                                                                                                                    <  1:383178/139‑1 (MQ=255)
        aTGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGGTTTTTCAGCCCCCGGGTCGCCAt                                                                                                                 <  1:369332/139‑1 (MQ=255)
        aTGTATTTGAAGCTGGGGGGCTTTTGCGCATCTGCAAGGTTTATCGGTTCCGCCCGCCTGCCAACCAGGCCGACGATCCCTTCTTCATACGCGAGCGTTACAGTGCGACCGCGTGGTTTTTCAGCACCCGGGTCGCCAt                                                                                                                 <  1:119782/139‑1 (MQ=255)
                     tGGGGTGCTTTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGGTTTTTCAGCCCCCGGGTCGCCATCAGGTAGTaacaa                                                                                                    >  2:178639/1‑139 (MQ=255)
                       gggTGCTTTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGGTTTTTCAGCCCCCGGGTCGCCAt                                                                                                                 >  1:246978/1‑124 (MQ=255)
                       gggTGCTTTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGGTTTTTCAGCCCCCGGGTCGCCAt                                                                                                                 <  2:246978/124‑1 (MQ=255)
                       gggTGCTTTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGGTTTTTCAGCCCCCGGGTCGCCATCAGGTAGTAACAACg                                                                                                  >  1:172398/1‑139 (MQ=255)
                       gggTGCTTTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGGTTTTTCAGCCCCCGGGTCGCCATCAGGTAGTAACAACg                                                                                                  <  1:33062/139‑1 (MQ=255)
                                tGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGGTTTTTCAGCCCCCGGGTCGCCATCAGGTAGTAACAACGTCGATCATg                                                                                         <  2:500716/139‑1 (MQ=255)
                                                 tATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGGTTTTTCAGCCCCCGGGTCGCCATCAGGTAGTAACAACGTCGATCATGATCGGCCAGGTAGACCg                                                                        >  1:134461/1‑139 (MQ=255)
                                                        tCCGCCAGGCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGGTTTTTCAGCCCCCGGGTCGCCATCAGGTAGTAACAACGTCGATCATGATCGGCCAGGTAGACCGAACAGAc                                                                 <  1:312282/139‑1 (MQ=255)
                                                                    ccAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGGTTTTTCAGCCCCCGGGTCGCCATCAGGTAGTAACAACGTCGATCATGATCGGCCAGGTAGACCGAACAGACCTCGGTATCCAt                                                     <  1:52753/139‑1 (MQ=255)
                                                                                      ccTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGGTTTTTCAGCCCCCGGGTCGCCATCAGGTAGTAACAACGTCGATCATGATCGGCCAGGTAGACCGAACAGACCTCGGTATCCATCGCAAGACAGATGTCGGt                                   >  1:397104/1‑139 (MQ=255)
                                                                                           atcaAACGCGAGCGTTACAGTGCGACCGCGTGGTTTTTCAGCCCCCGGGTCGCCATCAGGTAGTAACAACGTCGATCATGATCGGCCAGGTAGACCGAACAGACCTCGGTATCCATCGCAAGACAGATGTCGGTAACCa                              <  2:264253/139‑1 (MQ=255)
                                                                                                  gcgAGCGTTACAGTGCGACCGCGTGGTTTTTCAGCCCCCGGGTCGCCATCAGGTAGTAACAACGTCGATCATGATCGGCCAGGTAGACCGAACAGACCTCGGTATCCATCGCAAGACAGATGTCGGTAACCAGAATAtt                       >  1:429023/1‑139 (MQ=255)
                                                                                                   cgAGCGTTACAGTGCGACCGCGTGGTTTTTCAGCCCCCGGGt                                                                                                                       >  2:223165/1‑42 (MQ=255)
                                                                                                   cgAGCGTTACAGTGCGACCGCGTGGTTTTTCAGCCCCCGGGt                                                                                                                       <  1:223165/42‑1 (MQ=255)
                                                                                                       cGTTACAGTGCGACCGCGTGGTTTTTCAGCCCCCGGGTCGCCATCAGGTAGTAACAACGTCGATCATGATCGGCCAGGTAGACCGAACAGACCTCGGTATCCATCGCAAGACAGATGTCGGTAACCAGAATATTTAACg                  <  2:352168/139‑1 (MQ=255)
                                                                                                              gTGCGACCGCGTGGTTTTTCAGCCCCCGGGTCGCCATCAGGTAGTAACAACGTCGATCATGATCGGCCAGGTAGACCGAACAGACCTCGGTATCCATCGCAAGACAGATGTCGGTAACCAGAATATTTAACGCCTCAtt           <  2:158808/139‑1 (MQ=255)
                                                                                                               tGCGACCGCGTGGTTTTTCAGCCCCCGGGTCGCCATCAGGTAGTAACAACGTCGATCATGATCGGCCAGGTAGACCGAACAGACCTCGGTATCCATCGCAAGACAGATGTCGGTAACCAGAATATTTAACGCCTCATTc          >  1:418988/1‑139 (MQ=255)
                                                                                                                    ccGCGTGGTTTTTCAGCCCCCGGGTCGCCATCAGGTAGTAACAACGTCGATCATGATCGGCCAGGTAGACCGa                                                                       >  1:276465/1‑73 (MQ=255)
                                                                                                                    ccGCGTGGTTTTTCAGCCCCCGGGTCGCCATCAGGTAGTAACAACGTCGATCATGATCGGCCAGGTAGACCGa                                                                       <  2:276465/73‑1 (MQ=255)
                                                                                                                       cgTGGTTTTTCAGCCCCCGGGTCGCCATCAGGTAGTAACAACGTCGATCATGATCGGCCAGGTAGACCGAACAGACCTCGGTATCCATCGCAAGACAGATGTCGGTAACCAGAATATTTAACGCCTCATTCAGGCgtgg  >  1:468969/1‑139 (MQ=255)
                                                                                                                            |                                                                                                                                      
CGGAGGGGATGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGGTTTTTTCAGCCCCCGGGTCGCCATCAGGTAGTAACAACGTCGATCATGATCGGCCAGGTAGACCGAACAGACCTCGGTATCCATCGCAAGACAGATGTCGGTAACCAGAATATTTAACGCCTCATTCAGGCGTGG  >  NC_000913/2968131‑2968389

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: