Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,950,476 (T)8→7 intergenic (+56/‑31) ilvL → / → ilvX ilvG operon leader peptide/uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,950,4690T.100.0% 127.3 / NA 30intergenic (+49/‑38)ilvL/ilvXilvG operon leader peptide/uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (16/14);  total (16/14)

CTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGG  >  NC_000913/3950334‑3950596
                                                                                                                                       |                                                                                                                               
cTACGAGCAATTAGCCAGGTCGTGATTAGCGTGTTGGTGAATATTATCCCACGGTGCGGAGCTGCACTTGGATGAGGAAAAGCTTAGAGATCAAGCCTTAACGCACTACTACCCCCGCACCGAAAGATCTGGGGGtttt                                                                                                                              >  1:73659/1‑139 (MQ=255)
         aTTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTGACCtt                                                                                                                    >  2:82870/1‑139 (MQ=255)
                        aTTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTGACCTTAAAAACATAACCgag                                                                                                     >  1:183625/1‑139 (MQ=255)
                        aTTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTGACCTTAAAAACATAACCgag                                                                                                     <  2:183625/139‑1 (MQ=255)
                               tggtggtgATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGa                                                                                              <  1:107749/139‑1 (MQ=255)
                                 gtggtgATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACa                                                                                            >  1:214885/1‑139 (MQ=255)
                                  tggtgATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACaa                                                                                           >  2:294887/1‑139 (MQ=255)
                                                   ccGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACaaaa                                                                          >  1:42052/1‑139 (MQ=255)
                                                     gTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAAtt                                                                        >  2:450919/1‑139 (MQ=255)
                                                      tGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGa                                                                                              >  1:75275/1‑116 (MQ=255)
                                                      tGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGa                                                                                              <  2:75275/116‑1 (MQ=255)
                                                                cACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCaa                                                             >  2:89689/1‑139 (MQ=255)
                                                                     ggACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTGACCTTAAAAACATAAcc                                                                                                        <  1:461610/91‑1 (MQ=255)
                                                                     ggACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTGACCTTAAAAACATAAcc                                                                                                        >  2:461610/1‑91 (MQ=255)
                                                                      gACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCa                                                       <  1:187001/139‑1 (MQ=255)
                                                                              aaGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCTCCGAAAGGTCCGGGGGTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACgggg                                               <  1:89689/139‑1 (MQ=255)
                                                                                aggttAAAGATTAAACTTTAACGAAACAAGACCCCCGCAACGAAAGGTCCGGGGGTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGaa                                             <  1:99998/136‑1 (MQ=255)
                                                                                 gCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGaac                                            <  1:82870/139‑1 (MQ=255)
                                                                                 gCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGaac                                            <  1:450919/139‑1 (MQ=255)
                                                                                                  tAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGca                           <  1:15300/139‑1 (MQ=255)
                                                                                                   aaCGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTGACCTTAAAAACATAACCGTGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGcac                          >  2:304954/1‑139 (MQ=255)
                                                                                                   aaCGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGcac                          >  2:463024/1‑139 (MQ=255)
                                                                                                         cTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACa                                                                                  >  2:286566/1‑77 (MQ=255)
                                                                                                         cTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACa                                                                                  <  1:286566/77‑1 (MQ=255)
                                                                                                          tAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAgtgggt                   <  1:304954/139‑1 (MQ=255)
                                                                                                                  ccGCACCGAAAGGTCCGGGGGTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAgt                       <  2:380381/127‑1 (MQ=255)
                                                                                                                  ccGCACCGAAAGGTCCGGGGGTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAgt                       >  1:380381/1‑127 (MQ=255)
                                                                                                                          aaaGGTCCGGGGGTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGa                                                                                              <  1:441294/48‑1 (MQ=255)
                                                                                                                          aaaGGTCCGGGGGTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCgg   >  2:287538/1‑139 (MQ=255)
                                                                                                                          aaaGGTCCGGGGGCTTTTTTGACCTTAAAAACATAACCGAGGAGCAGa                                                                                              >  2:441294/1‑48 (MQ=255)
                                                                                                                           aaGGTCCGGGGGTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCggg  >  1:457541/1‑139 (MQ=255)
                                                                                                                                       |                                                                                                                               
CTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGCGTTGCGGG  >  NC_000913/3950334‑3950596

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: