Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,635,400 (T)7→6 intergenic (‑90/‑121) rob ← / → creA right oriC‑binding transcriptional activator, AraC family/putative periplasmic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,635,3940T.100.0% 91.2 / NA 22intergenic (‑84/‑127)rob/creAright oriC‑binding transcriptional activator, AraC family/putative periplasmic protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (12/10);  total (12/10)

ATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTCATCCTTTCAACAACGAGCACCTGACATCAGGTAATTGGATAATAGCATTTTTTGCTGTTTTAGCATTCAGTGTTTTTTTCTTAGTAGAGTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTATTTACCGTGACGAACTAATTGCTCGTGTAATAGATAAAAATGGTAACAATATGAAATA  >  NC_000913/4635260‑4635528
                                                                                                                                      |                                                                                                                                      
aTGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTCATCCTTTCAACAACGAGCACCTGACATCAGGTAATTGGATAATAGCATTTTTTGCTGTTTTAGCATTCAGTGttttt                                                                                                                                    <  2:413789/139‑1 (MQ=255)
      ttCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGACTGATACATAAAATATCCTCATCCTTTCAACAACGAGCACCTGACAGCAGGTAATTGGATAATAGCATTTTTTGCTGTTTTAGCATTCAGTG‑TTTTTTCTtag                                                                                                                             <  1:190563/139‑1 (MQ=255)
        ccagccagATTAAAAGGTCGCGAATAATGGCGGCCTGATCCATAAAATATCCTCATCCTTTCAACAACGAGCACCTGGCATCAGGTAATTGGATAATAGCATTTCTTGCTTTTTTAGCATTCAGTG‑TTTTTTCTtagta                                                                                                                           <  1:331469/139‑1 (MQ=255)
                          cgcgAATAATGCCGGCCTGATCCATAAAATATCCTCATCCTTTGAACAACGAGCACCTGACATCAGGTAATTGGATAATAGCATTTTTTGCTGTTTTACCATTCAGTGttt                                                                                                                                      >  2:343125/1‑111 (MQ=255)
                          cgcgAATAATGCCGGCCTGATCCATAAAATATCCTCATCCTTTCAACAACGAGCACCTGACATCAGGTAATTGGATAATAGCATTTTTTGCTGTTTTAGCATTCAGTGttt                                                                                                                                      <  1:343125/111‑1 (MQ=255)
                             gAATAATGCCGGCCTGATCCATAAAATATCCTCATCCTTTCAACAACGAGCACCTGACATCAGGTAATTGGATAATAGCATTTTTTGCTGTTTTAGCATTCAGTG‑TTTTTTCTTAGTAGAGTATGTTTTAGGGCTTGAc                                                                                                      <  2:201258/139‑1 (MQ=255)
                                                          ccTCATCCTTTCAACAACGAGCACCTGACATCAGGTAATTGGATAATAGCATTTTTTGCTGTTTTAGCATTCAgtgt                                                                                                                                        >  2:397589/1‑77 (MQ=255)
                                                          ccTCATCCTTTCAACAACGAGCACCTGACATCAGGTAATTGGATAATAGCATTTTTTGCTGTTTTAGCATTCAgtgt                                                                                                                                        <  1:397589/77‑1 (MQ=255)
                                                          ccTCATCCTTTCAACAACGAGCACCTGACATCAGGTAATTGGATAATAGCATTTTTTGCTGTTTTAGCATTCAGTG‑TTTTTTCTTAGTAGAGTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCtt                                                                         <  1:55717/139‑1 (MQ=255)
                                                           cTCATCCTTTCAACAAGTAGCACCTGACATCAGGTAATTGGATAATAGCATTTTTTGCTGTTTTAGCATTCAGTG‑TTTTTTCTTAGTAGAGTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTa                                                                        <  2:348400/139‑1 (MQ=255)
                                                              aTCCTTTCAACAACGAGCACCTGACATCAGGTAATTGGATAATAGCATTTTTTGCTGTTTTAGCATTCAGTGttttttctt                                                                                                                                >  1:438815/1‑78 (MQ=255)
                                                              aTCCTTTCAACAACGAGCACCTGACATCAGGTAATTGGATAATAGCATTTTTTGCTGTTTTAGCATTCAGTGttttttctt                                                                                                                                <  2:438815/81‑4 (MQ=255)
                                                                 cTTTCAACAACGAGCACCTGATATCAGGTAATTGGATAATAGCATTTTTTGCTGTTTTAGCATTCAGTG‑TTTTTTCTTAGTAGTGTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTGCTTAATCAat                                                                  <  1:483109/139‑1 (MQ=255)
                                                                   ttCAACAACGAGCACCTGACATCAGGTAATTGGATAATAGCATTTTTTGCTGTTTTAGCATTCAGTG‑TTTTTTCTTAGTAGAGTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAatat                                                                <  2:381311/139‑1 (MQ=255)
                                                                   ttCAACAACGAGCACCTGACATCAGGTAATTGGATAATAGCATTTTTTGCTGTTTTAGCATTCAGTG‑TTTTTTCTTAGTAGAGTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAatat                                                                <  2:240915/139‑1 (MQ=255)
                                                                    tCAACAACGAGCACCTGACATCAGGTAATTGGATAATAGCATTTTTTGCTGTTTTAGCATTCAGTG‑TTTTTTCTTAGTAGAGTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATg                                                               >  2:184319/1‑139 (MQ=255)
                                                                    tCAACAACGAGCACCTGACATCAGGTAATTGGATAATAGCATTTTTTGCTGTTTTAGCATTCAGTG‑TTTTTTCTTAGTAGAGTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATg                                                               >  2:222254/1‑139 (MQ=255)
                                                                       acaacGAGCACCTGACATCAGGTAATTGGATAATAGCATTTTTTGCTGTTTTAGCATTCAGTG‑TTTTTTCTTAGTAGAGTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTa                                                            >  1:39005/1‑139 (MQ=255)
                                                                             aGCACCTGACATCAGGTAATTGGATAATAGCATTTTTTGCTGTTTTAGCATTCAGTG‑TTTTTTCTTAGTAGAGTATGTTTTAGGGCTTGGCGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTATTTAcc                                                      >  1:513454/1‑139 (MQ=255)
                                                                             aGCACCTGACATCAGGTAATTGGATAATAGCATTTTTTGCTGTTTTAGCATTCAGTG‑TTTTTTCTTAGTAGAGTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGtt                                                             <  1:103941/132‑1 (MQ=255)
                                                                             aGCACCTGACATCAGGTAATTGGATAATAGCATTTTTTGCTGCTTTAGCATTCAGTG‑TTTTTTCTTAGTAGAGTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTATTTAcc                                                      >  1:11090/1‑139 (MQ=255)
                                                                             aGCACCTGACATCACGTAATTGGATAATAGCATTTTTTGCTGTTTTAGCATTCAGTG‑TTTTTTCTTAGTAGAGTATGTGTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTTATCAATATGtt                                                             >  2:103941/1‑132 (MQ=255)
                                                                                  cTGACATCAGGTAATTGGATAATAGCATTTTTTGCTGTTTTAGCATTCAGTG‑TTTTTTCTTAGTAGAGTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTATTTACCGTGAc                                                 >  2:122355/1‑139 (MQ=255)
                                                                                   tGACATCAGGTAATTGGATAATAGCATTTTTTGCTGTTTTAGCATTCAGTG‑TTTTTTCTTAGTAGAGTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTATTTACCGTGACg                                                <  1:222254/139‑1 (MQ=255)
                                                                                                     taataGCATTTTTTGCTGTTTTAGCATTCAGTG‑TTTTTTCTTAGTAGAGTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTATTTACCGTGACGAACTAATTGCTCGTGTaa                              >  2:161478/1‑139 (MQ=255)
                                                                                                          gCATTTTTTGCTGTTTTAGCATTCAGTG‑TTTTTTCTTAGTAGAGTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTATTTACCGTGACGAACTAATTGCTCGTGTAATAGAt                         >  2:239384/1‑139 (MQ=255)
                                                                                                           cATTTTTTGCTGTTTTAGCATTCAGTG‑TTTTTTCTTAGTAGAGTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTATTTACCGTGACGAACTAATTGCTCGTGTAATAGATa                        >  2:511378/1‑139 (MQ=255)
                                                                                                                          tAGCATTCAGTG‑TTTTTTCTTAGTAGAGTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTATTTACCGTGACGAACTAATTGCTCGTGTAATAGATAAAAATGGTAACAata         >  2:107869/1‑139 (MQ=255)
                                                                                                                                 cAGTG‑TTTTTTCTTAGTAGAGTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTATTTACCGTGACGAACTAATTGCTCGTGTAATAGATAAAAATGGTAACAATATGAAATa  >  2:87059/1‑139 (MQ=255)
                                                                                                                                      |                                                                                                                                      
ATGACCTTCCAGCCAGATTAAAAGGTCGCGAATAATGCCGGCCTGATCCATAAAATATCCTCATCCTTTCAACAACGAGCACCTGACATCAGGTAATTGGATAATAGCATTTTTTGCTGTTTTAGCATTCAGTGTTTTTTTCTTAGTAGAGTATGTTTTAGGGCTTGACGGAAATAAAAGTATTGAGATTTTGTTCTTAATCAATATGTTATTTACCGTGACGAACTAATTGCTCGTGTAATAGATAAAAATGGTAACAATATGAAATA  >  NC_000913/4635260‑4635528

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑