Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 783,566 (T)5→4 coding (401/720 nt) pnuC → nicotinamide riboside transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913783,5620T.100.0% 132.9 / NA 31coding (397/720 nt)pnuCnicotinamide riboside transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (8/23);  total (8/23)

TCGCGACAAACCAGTCAGAACGAGGCGGAGTTGAAAATTCGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGGCGGTTTGCGTTGTTTCGATTGGTCTGATGACGGTATTTATCAATCCGGTGTTTGCATTTTTGACCCGCGTGGCAGTCATGATCATGCAAGCATTAGGATTACAGGTTGTGATGCCTGAACTGCAACCGGACGCTTTCCCGTTCTGGGATTCATGCATGATGGTGTTATCTATCGTGGCAATGATTCTGATG  >  NC_000913/783430‑783696
                                                                                                                                    |                                                                                                                                      
tCGCGACAAACCAGTCAGAACGAGGCGGAGTTGAAAATTCGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGGCGGTTTGCGTTGTTTCGATTGGTCTGATGACGGTATTTATCAATCCGGTGTTTGCAttttgac                                                                                                                                  >  2:177506/1‑136 (MQ=255)
tCGCGACAAACCAGTCAGAACGAGGCGGAGTTGAAAATTCGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGGCGGTTTGCGTTGTTTCGATTGGTCTGATGACGGTATTTATCAATCCGGTGTTTGCAttttgac                                                                                                                                  >  2:429837/1‑136 (MQ=255)
 cgcgACAAACCAGTCAGAACGAGGCGGAGTTGAAAATTCGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGGCGGTTTGCGTTGTTTCGATTGGTCTGATGACGGTATTTATCAATCCGGTGTTTGCAttttgacc                                                                                                                                 >  2:266314/1‑135 (MQ=255)
 cgcgACAAACCAGTCAGAACGAGGCGGAGTTGAAAATTCGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGGCGGTTTGCGTTGTTTCGATTGGTCTGATGACGGTATTTATCAATCCGGTGTTTGCAttttgacc                                                                                                                                 >  1:371018/1‑135 (MQ=255)
   cgACAAACCAGTCAGACGGAGGCGGAGTTGAAAATTCGCTGGTGGCCATTGCCGAAGGCACTTAGCTGGTTGGCGGTTTGGGTTGTTTCGATTGATCTGATGACGGGATTTGTTTATCCGGTTGTTGTACTTTGACCcg                                                                                                                              <  1:324474/139‑1 (MQ=255)
   cgACAAACCAGTCAGAACGAGGCGGAGTTGAAAATTCGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGGCGGTTTGCGTTGTTTCGATTGGTCTGATGACGGTATTTATCAATCCGGTGTTTGCATTTTGACCcg                                                                                                                              <  1:389537/139‑1 (MQ=255)
      cAAACCAGTCAGAACGAGGCGGAGTTGAAAATTCGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGGCGGTTTGCGTTGTTTCGATTGGTCTGATGACGGTATTTATCAATCCGGTGTTTGCATTTTGACCCGCGt                                                                                                                           <  1:37949/139‑1 (MQ=255)
        aaCCAGTCAGAACGAGGCGGAGTTGAAAATTCGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGGCGGTTTGCGTTGTTTCGATTGGTCTGATGACGGTATTTATCAATCCGGTGTTTGCATTTTGACCCGCGTgg                                                                                                                         <  1:158545/139‑1 (MQ=255)
                      aGGCGGAGTTGAAAATTCGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGGCGGTTTGCGTTGTTTCGATTGGTCTGATGACGGTATTTATCAATCCGGTGTTTGCATTTTGACCCGCGTGGCAGTCATGATCATg                                                                                                           <  1:326656/139‑1 (MQ=255)
                       ggCGGAGTTGAAAATTCGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGGCGGTTTGCGTTGTTTCGATTGGTCTGATGACGGTATTTATCAATCCGGTGTTTGCATTTTGACCCGCGTgg                                                                                                                         <  1:257407/124‑1 (MQ=255)
                       ggCGGAGTTGAAAATTCGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGGCGGTTTGCGTTGTTTCGATTGGTCTGATGACGGTATTTATCAATCCGGTGTTTGCATTTTGACCCGCGTgg                                                                                                                         >  2:257407/1‑124 (MQ=255)
                             gTTGAAAATTCGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGGCGGTTTGCGTTGTTTCGATTGGTCTGATGACGGTATTTATCAATCCGGTGTTTGCATTTTGACCCGCGTGGCAGTCATGATCATGCAAGCAt                                                                                                    <  1:266314/139‑1 (MQ=255)
                                    aTTCGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGGCGGTTTGCGTTGTTTCGATTGGTCTGATGACGGTATTTATCAATCCGGTGTTTGCATTTTGACCCGCGTGGCAGTCATGATCATGCAAGCATTAGGAtt                                                                                             <  1:416600/139‑1 (MQ=255)
                                                                         tGGCGGTTTGCGTTGTTTCGATTGGTCTGATGACGGTATTTATCAATCCGGTGTTTGCATTTTGACCCGCGTGGCAGTCATGATCATGCAAGCATTAGGATTACAGGTTGTGATGCCTGAACTGCAACCGGACGCTTTc                                                        <  2:243344/139‑1 (MQ=255)
                                                                         tGGCGGTTTGCGTTGTTTCGATTGGTCTGATGACGGTATTTATCAATCCGGTGTTTGCATTTTGACCCGCGTGGCAGTCATGATCATGCAAGCATTAGGATTACAGGTTGTGATGCCTGAACTGCAACCGGACGCTTTc                                                        <  1:439346/139‑1 (MQ=255)
                                                                                  gCGTTGTTTCGATTGGTCTGATGACGGTATTTATCAATCCGGTGTTTGCATTTTGACCCGCGTGGCAGTCATGATCATGCAAGCATTAGGATTACAGGTTGTGATGCCTGAACTGCAACCGGACGCTTTCCCGTTCTgg                                               <  1:301408/139‑1 (MQ=255)
                                                                                       gttTCGATTGGTCTGATGACGGTATTTATCAATCCGGTGTTTGCATTTTGACCCGCGTGGCAGTCATGATCATGCAAGCATTAGGATTACAGGTTGTGATGCCTGAACTGCAACCGGACGCTTTCCCGTTCTGGGATTc                                          <  2:124425/139‑1 (MQ=255)
                                                                                        tttCGATTGGTCTGATGACGGTATTTATCAATCCGGTGTTTGCATTTTGACCCGCGTGGCAGTCATGATCATGCAAGCATTAGGATTACAGGTTGTGATGCCTGAACTGCAACCGGACGCTTTCCCGTTCTGGGATTca                                         <  1:225719/139‑1 (MQ=255)
                                                                                          tCGATTGGTCTGATGACGGTATTTATCAATCCGGTGTTTGCATTTTGACCCGCGTGGCAGTCATGATCATGCAAGCATTAGGATTACAGGTTGTGATGCCTGAACTGCAACCGGACGCTTTCCCGTTCTGGGATTcatg                                       <  2:371018/139‑1 (MQ=255)
                                                                                               tGGTCTGATGACGGTATTTATCAATCCGGTGTTTGCATTTTGACCCGCGTGGCAGTCATGATCATGCAAGCATTAGGATTACAGGTTGTGATGCCTGAACTGCAACCGGACGCTTTCCCGTTCTGGGATTCATGCatga                                  <  2:402690/139‑1 (MQ=255)
                                                                                                   cTGATGACGGTATTTATCAATCCGGTGTTTGCATTTTGACCCGCGTGGCAGTCATGATCATGCAAGCATTAGGATTACAGGTTGTGATGCCTGAACTGCAACCGGACGCTTTCCCGTTCTGGGATTCATGCATGATGgt                              <  1:177506/139‑1 (MQ=255)
                                                                                                   cTGATGACGGTATTTATCAATCCGGTGTTTGCATTTTGACCCGCGTGGCAGTCATGATCATGCAAGCATTAGGATTACAGGTTGTGATGCCTGAACTGCAACCGGACGCTTTCCCGTTCTGGGATTCATGCATGATGgt                              <  1:429837/139‑1 (MQ=255)
                                                                                                           ggTATTTATCAATCCGGTGTTTGCATTTTGACCCGCGTGGCAGTCATGATCATGCAAGCAtt                                                                                                   <  1:430949/62‑1 (MQ=255)
                                                                                                           ggTATTTATCAATCCGGTGTTTGCATTTTAACCCGCGTGGCAGTCATGATCATGCAAGCAtt                                                                                                   >  2:430949/1‑62 (MQ=255)
                                                                                                            gTATTTATCAATCCGGTGTTTGCATTTTGACCCGCGTGGCAGTCATGATCATGCAAGCATTAGGATTACAGGTTGTGATGCCTGAACTGCAACCGGACGCTTTCCCGTTCTGGGATTCATGCATGATGGTGTtatctat                     >  2:56175/1‑139 (MQ=255)
                                                                                                                     aaTACGGTGTTTGCATTTTGACCCGCGTGGCAGTCATGATCATGCAAGCATTAGGATTACAGGTTGTGATGCCTGAACTTCAACCGGACGCTTTCCCGTTCTGGGATTCATGCATGATGGTGTTATCTATCGTGGCAAt            <  1:41804/139‑1 (MQ=255)
                                                                                                                       tCCGGTGTTTGCATTTTGACCCGCGTGGCAGTCATGATCATGCAAGCATTAGGATTACAg                                                                                         <  1:413858/60‑1 (MQ=255)
                                                                                                                       tCCGGTGTTTGCATTTTGACCCGCGTGGCAGTCATGATCATGCAAGCATTAGGATTACAg                                                                                         >  2:413858/1‑60 (MQ=255)
                                                                                                                       tCCGGTGTTTGCATTTTGACCCGCGTGGCAGTCATGATCATGCAAGCATTAGGATTACAGGTTGTGATGCCTGAACTGCAACCGGACGCTTTCCCGTTCTGGGATTCATGCATGATGGTGTTATCTATCGTGGCAATGa          >  2:244055/1‑139 (MQ=255)
                                                                                                                       tCCGGTGTTTGCATTTTGACCCGCGTGGCAGTCATGATCATGCAAGCATTAGGATTACAGGTTGTGATGCCTGAACTGCAACCGGACGCTTTCCCGTTCTGGGATTCATGCATGATGGTGTTATCTATCGTGGCAATGa          >  2:370174/1‑139 (MQ=255)
                                                                                                                         cGGTGTTTGCATTTTGACCCGCGTGGCAGTCATGATCATGCAAGCATTAGGATTACAGGTTGTGATGCCTGAACTGCAACCGGACg                                                             >  2:387382/1‑86 (MQ=255)
                                                                                                                         cGGTGTTTGCATTTTGACCCGCGTGGCAGTCATGATCATGCAAGCATTAGGATTACAGGTTGTGATGCCTGAACTGCAACCGGACg                                                             <  1:387382/86‑1 (MQ=255)
                                                                                                                            tgtTTGCATTTTGACCCGCGTGGCAGTCATGATCATGCAAGCATTAGGATTACAGGTTGTGATGCCTGAACTGCAACCGGACGCTTTCCCGTTCTGGGATTCATGCATGATGGTGTTATCTATCGTGGCAATGATTCtg     >  1:394328/1‑139 (MQ=255)
                                                                                                                              tttGCATTTTGACCCGCGTGGCAGTCATGATCATGCAAGCATTAGGATTACAGGTTGTGATGCCTGAACTGCAACCGGACGCTTTCCCGTTCTGGGATTCATGCATGATGGTGTTATCTATCGTGGCAATGATTCtgat   <  2:352872/139‑1 (MQ=255)
                                                                                                                               ttGCATTTTGACCCGCGTGGCAGTCATGATCATGCAAGCATTAGGATTACAGGTTGTGATGCCTGAACTGCAACCGGACGCTTTCCCGTTCTGGGATTCATGCATGATGGTGTTATCTATCGTGGCAATGATTCtgatg  <  1:244055/139‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                      
TCGCGACAAACCAGTCAGAACGAGGCGGAGTTGAAAATTCGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGGCGGTTTGCGTTGTTTCGATTGGTCTGATGACGGTATTTATCAATCCGGTGTTTGCATTTTTGACCCGCGTGGCAGTCATGATCATGCAAGCATTAGGATTACAGGTTGTGATGCCTGAACTGCAACCGGACGCTTTCCCGTTCTGGGATTCATGCATGATGGTGTTATCTATCGTGGCAATGATTCTGATG  >  NC_000913/783430‑783696

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: