Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,240,435 T→C Q546R (CAG→CGG)  cvrA ← putative cation/proton antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,240,4350TC100.0% 117.0 / NA 31Q546R (CAG→CGG) cvrAputative cation/proton antiporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/16);  total (15/16)

AGCATCGCAACCGGCGATAGATGCTTCATTAAATTTAAGATTCAGCTTCCTCTTCAGCCACCCGTACGCCAATCTTCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGCCATATATCAGCGCAACATCAGCATATTTGGCGCT  >  NC_000913/1240301‑1240562
                                                                                                                                      |                                                                                                                               
aGCATCGCAACCGGCGATAGATGCTTCATTAAATTTAAGATTCAGCTTCCTCTTCAGCCACCCGTACGCCAATCTTCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTc                                                                                                                             >  2:176749/1‑139 (MQ=255)
                                               tCCTCTTCAGCCACCCGTACGCCAATCTTCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCa                                                                              >  1:303129/1‑139 (MQ=255)
                                                        gCCACCCGTACGCCAATCTTCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTtgct                                                                     <  2:172115/139‑1 (MQ=255)
                                                         ccACCCGTACGCCAATCTTCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGt                                                                                                                              <  2:513104/81‑1 (MQ=255)
                                                         ccACCCGTACGCCAATCTTCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGt                                                                                                                              >  1:513104/1‑81 (MQ=255)
                                                         ccACCCGTACGCCAATCTTCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTtgctg                                                                    <  1:166524/139‑1 (MQ=255)
                                                              cGTACGCCAATCTTCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTAt                                                               <  2:303129/139‑1 (MQ=255)
                                                              cGTACGCCAATCTTCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTAt                                                               >  1:208296/1‑139 (MQ=255)
                                                                        tCTTCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATtc                                                     >  2:385930/1‑139 (MQ=255)
                                                                                   tCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCAc                                                                                 >  1:483173/1‑100 (MQ=255)
                                                                                   tCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCAc                                                                                 <  2:483173/100‑1 (MQ=255)
                                                                                    cATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTc                                         <  2:18977/139‑1 (MQ=255)
                                                                                      ttATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCa                                       <  1:176749/139‑1 (MQ=255)
                                                                                      ttATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCa                                       <  2:20607/139‑1 (MQ=255)
                                                                                        aTCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAgg                                     <  2:40929/139‑1 (MQ=255)
                                                                                           ttctttctCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGCCa                                  <  1:385930/139‑1 (MQ=255)
                                                                                              tttctCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTtgc                                                                      >  1:457117/1‑100 (MQ=255)
                                                                                              tttctCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTtgc                                                                      <  2:457117/100‑1 (MQ=255)
                                                                                                 ctcGGCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTACTGCTTATCACGATATTCTCGCCCGTCTTCCAGGCCATATATc                            >  1:332806/1‑139 (MQ=255)
                                                                                                     gCCACCGTCCAGATCATCCCGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGCCATATATCAgcg                        <  2:208296/139‑1 (MQ=255)
                                                                                                                      cccGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTtgctg                                                                    <  1:61106/78‑1 (MQ=255)
                                                                                                                      cccGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTtgctg                                                                    >  2:61106/1‑78 (MQ=255)
                                                                                                                      cccGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGccc                                               <  1:118735/99‑1 (MQ=255)
                                                                                                                      cccGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGccc                                               >  2:118735/1‑99 (MQ=255)
                                                                                                                      cccGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTcc                                        <  1:97590/106‑1 (MQ=255)
                                                                                                                      cccGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTcc                                        >  2:97590/1‑106 (MQ=255)
                                                                                                                      cccGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAgg                                     <  2:110787/109‑1 (MQ=255)
                                                                                                                      cccGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAgg                                     >  1:110787/1‑109 (MQ=255)
                                                                                                                      cccGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGCCATATATCAGCGCAACATCAGCATATTTg       >  2:292140/1‑139 (MQ=255)
                                                                                                                      cccGGCAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGCCATATATCAGCGCAACATCAGCATATTTg       >  1:127151/1‑139 (MQ=255)
                                                                                                                           cAAACTCTACCCGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGCCATATATCAGCGCAACATCAGCATATTTGGCGCt  >  2:318486/1‑139 (MQ=255)
                                                                                                                                      |                                                                                                                               
AGCATCGCAACCGGCGATAGATGCTTCATTAAATTTAAGATTCAGCTTCCTCTTCAGCCACCCGTACGCCAATCTTCAACACTTCATTATCTTCTTTCTCGGCCACCGTCCAGATCATCCCGGCAAACTCTACCTGGTCACCGACAACCGGTGCCGCGCCTAACAACTGCTGGACAATTTCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGCCATATATCAGCGCAACATCAGCATATTTGGCGCT  >  NC_000913/1240301‑1240562

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: