Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,287,826	C→T	A267T (GCA→ACA)	purU ←	formyltetrahydrofolate hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,287,826	0	C	T	100.0%	78.2 / NA	23	A267T (GCA→ACA)	purU	formyltetrahydrofolate hydrolase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (10/13);  total (10/13)

GCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATT  >  NC_000913/1287690‑1287958                                                                                                                                         |                                                                                                                                     gccgcTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCa                                                                                                                                    <  1:216846/139‑1 (MQ=255) gccgcTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCa                                                                                                                                    <  1:129859/139‑1 (MQ=255) gccgcTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCa                                                                                                                                    <  2:274894/139‑1 (MQ=255)           aGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGta                                                                                                                          <  1:43341/139‑1 (MQ=255)                  cGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAGCGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCa                                                                                                                   >  2:235822/1‑139 (MQ=255)                  cGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCa                                                                                                                   <  1:211597/139‑1 (MQ=255)                       agaAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTTGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACt                                                                                                              >  2:338921/1‑139 (MQ=255)                       agaAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGta                                                                                                                          >  1:195926/1‑127 (MQ=255)                       agaAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGta                                                                                                                          <  2:195926/127‑1 (MQ=255)                                           tAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGt                                                                                          >  1:483696/1‑139 (MQ=255)                                                              aTTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTTTAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTgcgc                                                                              <  1:362486/132‑1 (MQ=255)                                                              aTTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTgcgc                                                                              >  2:362486/1‑132 (MQ=255)                                                                      tAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAg                                                               <  1:296967/139‑1 (MQ=255)                                                                        aCCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCt                                                             >  2:69980/1‑139 (MQ=255)                                                                                       tGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCg                                              >  2:74535/1‑139 (MQ=255)                                                                                                   aataatCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACg                                  <  2:483696/139‑1 (MQ=255)                                                                                                               aTTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCatgatg                      >  1:90247/1‑139 (MQ=255)                                                                                                                  aCCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGAtt                   <  1:69980/139‑1 (MQ=255)                                                                                                                      tAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGc               >  2:106115/1‑139 (MQ=255)                                                                                                                             aGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATGTCTTCAGCTGTGTAGGTATGa                                                 >  2:86932/1‑98 (MQ=255)                                                                                                                             aGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATGTCTTCAGCTGTGTAGGTATGa                                                 <  1:86932/98‑1 (MQ=255)                                                                                                                                   gcTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGAtt  <  1:86950/139‑1 (MQ=255)                                                                                                                                   gcTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGAtt  <  1:235822/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                                     GCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATT  >  NC_000913/1287690‑1287958

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑