Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,426,252 A→G intergenic (+170/‑202) ydaY → / → tmpR pseudogene, Rac prophage,Phage or Prophage Related/Rac prophage, pseudogene, tail protein family,Phage or Prophage Related, putative alpha helix protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,426,2520AG98.2% 204.7 / NA 54intergenic (+170/‑202)ydaY/tmpRpseudogene, Rac prophage,Phage or Prophage Related/Rac prophage, pseudogene, tail protein family,Phage or Prophage Related, putative alpha helix protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (23/30);  minor base T (0/1);  total (23/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

GGTAATATCCCGTGCCCGACGTTATGCTGGAATGGCTGGGGTGCCTTTGCCTTTATCTCTGACAGAGATAAATGAATATTTAGCCACTCATCCGGTATTGATTGAGCGCGATGAATTTGAAGCAGTGATCTTTGCACTGGATGACCAGTATTTTCAGGAGCAGTGTGTGTAGTTGTTAATTACGTACACTCTGTTACAGAGATGTGATGGTGTCTTTAATTAAATCGATGATGCTCCTGGAGAAAAGCATTGCGTGGCCTCGTAATCGCT  >  NC_000913/1426117‑1426386
                                                                                                                                       |                                                                                                                                      
ggTAATATCCCGTGCCCGACGTTATGCTGGAATGGCTGGGGTGCCTTTGCCTTTATCTCTGACAGAGATAAATGAATATTTAGCCACTCATCCGGTATTGATTGAGCGCGATGAATTTGAAGCAGTGATCTTTGCGCTg                                                                                                                                     <  1:169791/139‑1 (MQ=255)
  tAATATCCCGTGCCCGACGTTATGCTGGAATGGCTGGGGTGCCTTTGCCTTTATCTCTGACAGAGATAAATGAATATTTAGCCACTCATCCGGTATTGATTGAGCGCGATGAATTTGAAGCAGTGATCTTTGCGCTGGa                                                                                                                                   >  2:92069/1‑139 (MQ=255)
      atCCCGTGCCCGACGTTATGCTGGAATGGCTGGGGTGCCTTTGCCTTTATCTCTGACAGAGATAAATGAATATTTAGCCACTCATCCGGTATTGATTGAGCGCGATGAATTTGAAGCAGTGATCTTTGCGCTGGATGAc                                                                                                                               <  2:51541/139‑1 (MQ=255)
            tGCCCGACGTTATGCTGGAATGGCTGGGGTGCCTTTGCCTTTATCTCTGACAGAGATAAATGAATATTTAGCCACTCATCCGGTATTGATTGAGCGCGATGAATTTGAAGCAGTGATCTTTGCGCTGGATGACCAGTAt                                                                                                                         >  1:210766/1‑139 (MQ=255)
            tGCCCGACGTTATGCTGGAATGGCTGGGGTGCCTTTGCCTTTATCTCTGACAGAGATAAATGAATATTTAGCCACTCATCCGGTATTGATTGAGCGCGATGAATTTGAAGCAGTGATCTTTGCGCTGGATGACCAGTAt                                                                                                                         >  1:402793/1‑139 (MQ=255)
               ccGACGTTATGCTGGAATGGCTGGGGTGCCTTTGCCTTTATCTCTGACAGAGATAAATGAATATTTAGCCACTCATCCGGTATTGATTGAGCGCGATGAATTTGAAGCAGTGATCTTTGCGCTGGATGACCAGTAtttt                                                                                                                      <  1:353582/139‑1 (MQ=255)
                   cGTTATGCTGGAATGGCTGGGGTGCCTTTGCCTTTATCTCTGACAGAGATAAATGAATATTTAGCCACTCATCCGGTATTGATTGAGCGCGATGAATTTGAAGCAGTGATCTTTGCGCTGGATGACCAGTATTTTCAgg                                                                                                                  >  1:437227/1‑139 (MQ=255)
                       aTGCTGGAATGGCTGGGGTGCCTTTGCCTTTATCTCTGACAGAGATAAATGAATATTTAGCCACTCATCCGGTATTGATTGAGCGCGATGAATTTGAAGCAGTGATCTTTGCGCTGGATGACCAGTATTTTCAGGAGCa                                                                                                              <  1:209151/139‑1 (MQ=255)
                              aaTGGCTGGGGTGCCTTTGCCTTTATCTCTGACTGAGATAAATGAATATTTAGCCACTCATCCGGTATTGATTGAGCGCGATGAATTTGAAGCAGTGATCTTTGCGCTGGATGACCAGTATTTTCAGGAGCAgtgtgtg                                                                                                       >  1:439112/1‑139 (MQ=255)
                              aaTGGCTGGGGTGCCTTTGCCTTTATCTCTGACAGAGATAAATGAATATTTAGCCACTCATCCGGTATTGATTGAGCGCGATGAATTTGAAGCAGTGATCTTTGCGCTGGATGACCAGTATTTTCAGGAGCAgtgtgtg                                                                                                       <  2:112877/139‑1 (MQ=255)
                                     ggggTGCCTTTGCCTTTATCTCTGACAGAGATAAATGAATATTTAGCCACTCATCCGGTATTGATTGAGCGCGATGAATTTGAAGCAGTGATCTTTGCGCTGGATGACCAGTATTTTCAGGAGCAGTGTGTGTAgttgt                                                                                                <  1:92069/139‑1 (MQ=255)
                                       ggTGCCTTTGCCTTTATCTCTGACAGAGATAAATGAATATTTAGCCACTCATCCGGTATTGATTGAGCGCGATGAATTTGAAGCAGTGATCTTTGCGCTGGATGACCAGTATTTTCAGGAGCAGTGTGTGTAGTTGTTa                                                                                              <  1:264321/139‑1 (MQ=255)
                                        gTGCCTTTGCCTTTATCTCTGACTGAGATAAATGAATATTTAGCCACTCATCCGGTATTGATTGAGCGCGATGAATTTGAAGCAGTGATCTTTGCGCTGGATGACCAGTATTTTCAGGAGCAGTGTGTGTAGTTGTTaa                                                                                             <  2:439112/139‑1 (MQ=255)
                                         tgcctttgccttTATCTCTGACAGAGATAAATGAATATTTAGCCACTCATCCGGTATTGATTGAGCGCGATGAATTTGAAGCAGTGATCTTTGCGCTGGATGACCAGTATTTTCAGGAGCAGTGTGTGTAGTTGTTAAt                                                                                            <  2:172346/139‑1 (MQ=255)
                                            ctttgcctttATCTCTGACAGAGATAAATGAATATTTAGCCACTCATCCGGTATTGATTGAGCGCGATGAATTTGAAGCAGTGATCTTTGCGCTGGATGACCAGTATTTTCAGGAGCAGTGTGTGTAGTTGTTAATTAc                                                                                         >  2:24042/1‑139 (MQ=255)
                                            ctttgcctttATCTCTGACAGAGATAAATGAATATTTAGCCACTCATCCGGTATTGATTGAGCGCGATGAATTTGAAGCAGTGATCTTTGCGCTGGATGACCAGTATTTTCAGGAGCAGTGTGTGTAGTTGTTAATTAc                                                                                         >  2:300085/1‑139 (MQ=255)
                                                   tttATCTCTGACAGAGATAAATGAATATTTAGCCACTCATCCGGTATTGATTGAGCGCGATGAATTTGAAGCAGTGATCTTTGCGCTGGATGACCAGTATTTTCAGGAGCAGTGTGTGTAGTTGTTAATTACGTACAct                                                                                  <  1:100172/139‑1 (MQ=255)
                                                   tttATCTCTGACAGAGATAAATGAATATTTAGCCACTCATCCGGTATTGATTGAGCGCGATGAATTTGAAGCAGTGATCTTTGCGCTGGATGACCAGTATTTTCAGGAGCAGTGTGTGTAGTTGTTAATTACGTACAct                                                                                  >  2:100172/1‑139 (MQ=255)
                                                   tttATCTCTGACAGAGATAAATGAATATTTAGCCACTCATCCGGTATTGATTGAGCGCGATGAATTTGAAGCAGTGATCTTTGCGCTGGATGACCAGTATTTTCAGGAGCAGTGTGTGTAGTTGTTAATTACGTACAct                                                                                  <  1:512270/139‑1 (MQ=255)
                                                   tttATCTCTGACAGAGATAAATGAATATTTAGCCACTCATCCGGTATTGATTGAGCGCGATGAATTTGAAGCAGTGATCTTTGCGCTGGATGACCAGTATTTTCAGGAGCAGTGTGTGTAGTTGTTAATTACGTACAct                                                                                  <  1:370044/139‑1 (MQ=255)
                                                   tttATCTCTGACAGAGATAAATGAATATTTAGCCACTCATCCGGTATTGATTGAGCGCGATGAATTTGAAGCAGTGATCTTTGCGCTGGATGACCAGTATTTTCAGGAGCAGTGTGTGTAGTTGTTAATTACGTACAct                                                                                  >  2:370044/1‑139 (MQ=255)
                                                    ttATCTCTGACAGAGATAAATGAATATTTAGCCACTCATCCGGTATTGATTGAGCGCGATGAATTTGAAGCAGTGATCTTTGCGCTGGATGACCAGTATTTTCAGGAGCAGTGTGTGTAGTTGTTAATTACGTACActc                                                                                 <  2:437227/139‑1 (MQ=255)
                                                     tATCTCAGACAGAGATAAATGAATATTTAGCCACTCATCCGGTATTGATTGAGCGCGATGAATTTGAAGCAGTGATCTTTGCGCTGGATGACCAGTATTTTCAGGAGCAGTGTGTGTAGTTGTTAATTACGTACActct                                                                                >  2:361352/1‑139 (MQ=255)
                                                         tctGACAGAGATAAATGAATATTTAGCCACTCATCCGGTATTGATTGAGCGCGATGAATTTGAAGCAGTGATCTTTGCGCTGGATGACCAGTATTTTCAGGAGCAGTGTGTGTAGTTGTTAATTACGTACACTCTGTTa                                                                            <  2:402793/139‑1 (MQ=255)
                                                         tctGACAGAGATAAATGAATATTTAGCCACTCATCCGGTATTGATTGAGCGCGATGAATTTGAAGCAGTGATCTTTGCGCTGGATGACCAGTATTTTCAGGAGCAGTGTGTGTAGTTGTTAATTACGTACACTCTGTTa                                                                            <  1:208134/139‑1 (MQ=255)
                                                                 agaTAAATGAATATTTAGCCACTCATCCGGTATTGATTGAGCGCGATGAATTTGAAGCAGTGATCTTTGCGCTGGATGACCAGTATTTTCAGGAGCAGTGTGTGTAGTTGTTAATTACGTACACTCTGTTACAGAGAtg                                                                    >  2:338330/1‑139 (MQ=255)
                                                                   aTAAATGAATATTTAGCCACTCATCCGGTATTGATTGAGCGCGATGAATTTGAAGCAGTGATCTTTGCGCTGGATGACCAGTATTTTCAGGAGCa                                                                                                              <  1:279594/95‑1 (MQ=255)
                                                                   aTAAATGAATATTTAGCCACTCATCCGGTATTGATTGAGCGCGATGAATTTGAAGCAGTGATCTTTGCGCTGGATGACCAGTATTTTCAGGAGCa                                                                                                              >  2:279594/1‑95 (MQ=255)
                                                                            tatTTAGCCACTCATCCGGTATTGATTGAGCGCGATGAATTTGAAGCAGTGATCTTTGCGCTGGATGACCAGTATTTTCAGGAGCAGTGTGTGTAGTTGTTAATTACGTACACTCTGTTACAGAGATGTGATGGTGTCt                                                         >  1:23500/1‑139 (MQ=255)
                                                                            tatTTAGCCACTCATCCGGTATTGATTGAGCGCGATGAATTTGAAGCAGTGATCTTTGCGCTGGATGACCAGTATTTTCAGGAGCAGTGTGTGTAGTTGTTAATTACGTACACTCTGTTACAGAGATGTGATGGTGTCt                                                         >  1:345287/1‑139 (MQ=255)
                                                                             atTTAGCCACTCATCCGGTATTGATTGAGCGCGATGAATTTGAAGCAGTGATCTTTGCGCTGGATGACCAGTATTTTCAGGAGCAGTGTGTGTAGTTGTTAATTACGTACACTCTGTTACAGAGATGTGATg                                                               <  1:22389/132‑1 (MQ=255)
                                                                             atTTAGCCACTCATCCGGTATTGATTGAGCGCGATGAATTTGAAGCAGTGATCTTTGCGCTGGATGACCAGTATTTTCAGGAGCAGTGTGTGTAGTTGTTAATTACGTACACTCTGTTACAGAGATGTGATg                                                               >  2:22389/1‑132 (MQ=255)
                                                                                       tCATCCGGTATTGATTGAGCGCGATGAATTTGAAGCAGTGATCTTTGCGCTGGATGACCAGTATTTTCAGGAGCAgt                                                                                                            >  1:85366/1‑77 (MQ=255)
                                                                                       tCATCCGGTATTGATTGAGCGCGATGAATTTGAAGCAGTGATCTTTGCGCTGGATGACCAGTATTTTCAGGAGCAgt                                                                                                            <  2:85366/77‑1 (MQ=255)
                                                                                                 ttgattgaGCGCGATGAATTTGAAGCAGTGATCTTTGCGCTGGATGACCAGTATTTTCAGGAGCAGTGTGTGTAGTTGTTAATTACGTACACTCTGTTACAGAGATGTGATGGTGTCTTTAATTAAATCGATGATGCTc                                    >  2:46538/1‑139 (MQ=255)
                                                                                                        aGCGCGATGAATTTGAAGCAGTGATCTTTGCGCTGGATGACCAGTATTTTCAGGAGCAGTGTGTGTAGTTGTTAATTACGTACACTCTGTTACAGAGATGTGATGGTGTCTTTAATTAAATCgatgat                                        >  2:279991/1‑128 (MQ=255)
                                                                                                        aGCGCGATGAATTTGAAGCAGTGATCTTTGCGCTGGATGACCAGTATTTTCAGGAGCAGTGTGTGTAGTTGTTAATTACGTACACTCTGTTACAGAGATGTGATGGTGTCTTTAATTAAATCgatgat                                        <  1:279991/128‑1 (MQ=255)
                                                                                                        aGCGCGATGAATTTGAAGCAGTGATCTTTGCGCTGGATGACCAGTATTTTCAGGAGCAGTGTGTGTAGTTGTTAATTACGTACACTCTGTTACAGAGATGTGATGGTGTCTTTAATTAAATCGATGATGCTCCTGgaga                             >  1:292814/1‑139 (MQ=255)
                                                                                                         gcgcGATGAATTTGAAGCAGTGATCTTTGCGCTGGATGACCAGTATTTTCAGGAGc                                                                                                               <  1:37041/56‑1 (MQ=255)
                                                                                                         gcgcGATGAATTTGAAGCAGTGATCTTTGCGCTGGATGACCAGTATTTTCAGGAGc                                                                                                               >  2:37041/1‑56 (MQ=255)
                                                                                                         gcgcGATGAATTTGAAGCAGTGATCTTTGCGCTGGATGACCAGTATTTTCAGGAGCAGTGTGTGTAGTTGTTa                                                                                              <  1:123762/73‑1 (MQ=255)
                                                                                                         gcgcGATGAATTTGAAGCAGTGATCTTTGCGCTGGATGACCAGTATTTTCAGGAGCAGTGTGTGTAGTTGTTa                                                                                              >  2:123762/1‑73 (MQ=255)
                                                                                                         gcgcGATGAATTTGAAGCAGTGATCTTTGCGCTGGATGACCAGTATTTTCAGGAGCAGTGTGTGTAGTTGTTAATTACGTACACTCTGTTACAGAGATGTGATGGTGTCTTTAATTAAATCGATGATGCTcc                                   >  1:211101/1‑132 (MQ=255)
                                                                                                         gcgcGATGAATTTGAAGCAGTGATCTTTGCGCTGGATGACCAGTATTTTCAGGAGCAGTGTGTGTAGTTGTTAATTACGTACACTCTGTTACAGAGATGTGATGGTGTCTTTAATTAAATCGATGATGCTcc                                   <  2:211101/132‑1 (MQ=255)
                                                                                                            cgATGAATTTGAAGCAGTGATCTTTGCGCTGGATGACCAGTATTTTCAGGAGCAGTGTGTGTAGTTGTTAATTACGTACACTCTGTTACAGAGATGTGATGGTGTCTTTAATTAAATCGATGATGCTCCTGGAGAAAAg                         <  2:292814/139‑1 (MQ=255)
                                                                                                                gAATTTGAAGCAGCGATCTTTGCTCTGGATGACCAGTATTTTCAGGAGCAGTGTGTGTAGTTGTTAATTACGTACACTCCGTTACAGAGATGTGATGGTCTCTTTAATTAAATCGATGATGCTCCTGGAGAAAAGCAtt                     <  1:338330/139‑1 (MQ=255)
                                                                                                                             tGATCTTTGCGCTGGATGACCAGTATTTTCAGGAGCAGTGTGTGTAgtt                                                                                                  <  2:369309/49‑1 (MQ=255)
                                                                                                                             tGATCTTTGCGCTGGATGACCAGTATTTTCAGGAGCAGTGTGTGTAgtt                                                                                                  >  1:369309/1‑49 (MQ=255)
                                                                                                                             tGATCTTTGCGCTGGATGACCAGTATTTTCAGGAGCAGTGTGTGTAGTTGTTAATTACGTACACTCTGTTACAGAGATGTGATGGTGTCTTTAAATAAATCGATGATGCTCCTGGAGAAAAGCATTGCGTGGCCTCGTa        <  1:361352/139‑1 (MQ=255)
                                                                                                                              gATCTTTGCGCTGGATGACCAGTATTTTCAGGAGCAGTGTGTGTAGTTGTTAATTACGTACACTCTGTTACAGAGATGTGATGGTGTCTTTAATTAAATCGATGATGCTCCTGGAGAAAAGCATTGCGTGGCCTCGTaa       <  2:401549/139‑1 (MQ=255)
                                                                                                                                   ttGCGCTGGATGACCAGTCTTTTCAGGAGCAGTGTGTGTAGTTGTTAATTACGTACACTCTGTTACAGAGATGTGATGGTGTCTTTAATTAAATCGATGATGCTCCTGGAGAAAAGCATTGCGTGGCCTCGTAATCGCt  <  2:345287/139‑1 (MQ=255)
                                                                                                                                   ttGCGCTGGATGACCAGTATTTTCAGGAGCAGTGTGTGTAGTTGTTAATTACGTACACTCTGTTACAGAGATGTGATGGTGTCTTTAATTAAATCGATGATGCTCCTGGAGAAAAGCATTGCGTGGCCTCGTAATCGCt  <  1:24042/139‑1 (MQ=255)
                                                                                                                                   ttGCGCTGGATGACCAGTATTTTCAGGAGCAGTGTGTGTAGTTGTTAATTACGTACACTCTGTTACAGAGATGTGATGGTGTCTTTAATTAAATCGATGATGCTCCTGGAGAAAAGCATTGCGTGGCCTCGTAATCGCt  <  2:124732/139‑1 (MQ=255)
                                                                                                                                   ttGCGCTGGATGACCAGTATTTTCAGGAGCAGTGTGTGTAGTTGTTAATTACGTACACTCTGTTACAGAGATGTGATGGTGTCTTTAATTAAATCGATGATGCTCCTGGAGAAAAGCATTGCGTGGCCTCGTAATCGCt  <  1:300085/139‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                      
GGTAATATCCCGTGCCCGACGTTATGCTGGAATGGCTGGGGTGCCTTTGCCTTTATCTCTGACAGAGATAAATGAATATTTAGCCACTCATCCGGTATTGATTGAGCGCGATGAATTTGAAGCAGTGATCTTTGCACTGGATGACCAGTATTTTCAGGAGCAGTGTGTGTAGTTGTTAATTACGTACACTCTGTTACAGAGATGTGATGGTGTCTTTAATTAAATCGATGATGCTCCTGGAGAAAAGCATTGCGTGGCCTCGTAATCGCT  >  NC_000913/1426117‑1426386

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: