| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,467,061 | C→T | pseudogene (1670/2513 nt) | ydbA → | pseudogene, autotransporter homolog, interrupted by IS2 and IS30 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,467,061 | 0 | C | T | 97.2% | 117.8 / NA | 36 | pseudogene (1670/2513 nt) | ydbA | pseudogene, autotransporter homolog, interrupted by IS2 and IS30 |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (12/23); minor base G (1/0); total (13/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.61e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGTACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCATGGTGTTGAGAACATTGGTGACAACGGCACGGTTAACAACAAAGGAGATATTGTTGTTTCCGATACTGGATCGATTGGCGTGCTCATCAACGGTGAGGGGGCAACAGTATCCAATACGGGTGATGTTAA > NC_000913/1466929‑1467193 | tCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGTACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGTGCATgg < 2:388069/139‑1 (MQ=255)tCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGTACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGTGCATgg < 1:404750/139‑1 (MQ=255) cAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGTACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGTGCATGgt < 2:162026/139‑1 (MQ=255) acgacgCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGTACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGTGCATGGTGTTGAGaa > 2:314716/1‑139 (MQ=255) aCCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGTACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGTGCAt < 1:203333/120‑1 (MQ=255) aCCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGTACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGTGCAt > 2:203333/1‑120 (MQ=255) aCCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGTACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGTGCATGGTGTTGAGAACATTGGTg > 2:218627/1‑139 (MQ=255) cAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGTACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGTGCATGGTGTTGAGAACATTGGTGACAACGGCACg > 2:45074/1‑139 (MQ=255) tGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGTACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGTGCATGGTGTTGAGAACATTGGTGACAACGGCACGGtt < 2:28971/139‑1 (MQ=255) aaCCACTGTTGATGGAAAAGATTCCACGGGTACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGTGCATGGTGTTGAGAACATTGGTGACAACGGCACGGTTaacaac < 1:94243/139‑1 (MQ=255) aCTGTTGATGGAAAAGATTCCACGGGTACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGTGCATGGTGTTGAGAACATTGGTGACAACGGCACGGTTAACAACAAAg > 2:231731/1‑139 (MQ=255) aCTGTTGATGGAAAAGATTCCACGGGTACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGTGCATGGTGTTGAGAACATTGGTGACAACGGCACGGTTAACAACAAAg > 2:470716/1‑139 (MQ=255) cTGTTGATGGAAAAGATTCCACGGGTACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGTGCATGGTGTTGAGAACATTGGTGACAACGGCACGGTTAACAACAAAgg < 2:218970/139‑1 (MQ=255) tGTTGATGGAAAAGATTCCACGGGTACCAAAATCGCGGGCAATATCGGCCTTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGGGCATGGTGTTGAGAACATTGGTGACAACGGCACGGTTAACAACAAAGga > 1:325622/1‑139 (MQ=255) tGGAAAAGATTCCACGGGTACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGTGCATGGTGTTGAGAACATTGGTGACAACGGCACGGTTAACAACAAAGGAGATAtt < 2:156793/139‑1 (MQ=255) aTTCCACGGGTACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGTGCATGGTGTTGAGAACATTGGTGACAACGGCACGGTTAACAACAAAGGAGATATTGTTGTTTc < 1:314716/139‑1 (MQ=255) aTTCCACGGGTACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGTGCATGGTGTTGAGAACATTGGTGACAACGGCACGGTTAACAACAAAGGAGATATTGTTGTTTc < 2:325622/139‑1 (MQ=255) gggCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGTGCATGGTGTTGAGAACATTGGTGATAACGGCACGGTTAACAACAAAGGAGATATTGTTGTTTCCGATACTGGATCGATTGGCGTg < 1:25653/139‑1 (MQ=255) gTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGTGCATGGTGTTGAGAACATTGGTGACAACGGCACGGTTAACAACAAAGGAGATATTGTTGTTTCCGATACTGGATCGATTGGCGTGCTCATCAACGGTGAgg > 2:187490/1‑139 (MQ=255) aaTCTGGATGGTAGCCTGACTGTTACAGGCGGTGTGCATGGTGTTGAGAACATTGGTGACAACGGCACGGTTAACAACAAAGGAGATATTGTTGTTTCCGATACTGGATCGATTGGCGTGCTCATCAACGGTGAggggg < 1:470716/139‑1 (MQ=255) aaTCTGGATGGTAGCCTGACTGTTACAGGCGGTGTGCATGGTGTTGAGAACATTGGTGACAACGGCACGGTTAACAACAAAGGAGATATTGTTGTTTCCGATACTGGATCGATTGGCGTGCTCATCAACGGTGAggggg < 1:45074/139‑1 (MQ=255) aaTCTGGATGGTAGCCTGACTGTTACAGGCGGTGTGCATGGTGTTGAGAACATTGGTGACAACGGCACGGTTAACAACAAAGGAGATATTGTTGTTTCCGATACTGGATCGATTGGCGTGCTCATCAACGGTGAggggg < 1:231731/139‑1 (MQ=255) aaTCTGGATGGTAGCCTGACTGTTACAGGCGGTGTGCATGGTGTTGAGAACATTGGTGACAACGGCACGGTTAACAACAAAGGAGATATTGTTGTTTCCGATACTGGATCGATTGGCGTGCTCATCAACGGTGAggggg < 1:165634/139‑1 (MQ=255) cTGGATGGTAGCCTGACTGTTACAGGCGGTGTGCATGGTGTTGAGAACATTGGTGACAACGGCACGGTTAACAACAAAGGAGATATTGTTGTTTCCGATACTGGATCGATTGGCGTGCTCATCAACGGTGAGGGGGCaa < 1:218627/139‑1 (MQ=255) cTGGATGGTAGCCTGACTGTTACAGGCGGTGTACATGGTGTTGAGAACATTGGTGACAACGGCACGGTTAACAACAAAGGAGATATTGTTGTTTCCGATACTGGATCGATTGGCGTGCTCATCAACGGTGAGGGGGCaa < 2:158807/139‑1 (MQ=255) ggATGGTAGCCTGACTGTTACAGGCGGTGTGCATGGTGTTGAGAACATTGGTGACAACGGCACGGTTAACAACAAAGGAGATATTGTTGTTTCCGATACTGGATCGATTGGCGTGCTCATCAACGGTGAGGGGGCAACa < 1:186794/139‑1 (MQ=255) gATGGTAGCGTGACTGTTACAGGCGGTGTGCATGGTGTTGAGAACATTGGTGACAACGGCACGGTTAACAACAAAGGAGATATTGTTGTTTCCGATACTGGATCGATTGGCGTGCTCATCAACGGTGAGGGGGCAACAg > 1:512126/1‑139 (MQ=255) gATGGTAGCCTGACTGTTACAGGCGGTGTGCATGGTGTTGAGAACATTGGTGACAACGGCACGGTTAACAACAAAGGAGata < 2:15679/82‑1 (MQ=255) gATGGTAGCCTGACTGTTACAGGCGGTGTGCATGGTGTTGAGAACATTGGTGACAACGGCACGGTTAACAACAAAGGAGata > 1:15679/1‑82 (MQ=255) tGGTAGCCTGACTGTTACAGGCGGTGTGCATGGTGTTGAGAACATTGGTGACAACGGCACGGTTAACAACAAAGGAGATATTGTTGTTTCCGATACTGGATCGATTGGCGTGCTCATCAACGGTGAGGGGGCAACAGTa < 2:150277/139‑1 (MQ=255) ccTGACTGTTACAGGCGGTGTGCATGGTGTTGAGAACATTGGTGACAACGGCACGGTTAACAACAAAGGAGATATTGTTGTTTCCGATACTGGATCGATTGGCGTGCTCATCAACGGTGAGGGGGCAACAGTATCCAAt > 1:53364/1‑139 (MQ=255) aCAGGCGGTGTGCATGGTGTTGAGAACATTGGTGACAACGGCACGGTTAACAACAAAGGAGATATTGTTGTTTCCGATACTGGATCGATTGGCGTGCTCATCAACGGTGAGGGGGCAACAGTATCCAATACGGGTGATg < 2:316111/139‑1 (MQ=255) aGGCGGTGTGCATGGTGTTGAGAACATTGGTGACAACGGCACGGTTAACAACAAAGGAGATATTGTTGTTTCCGATACTGGATCGATTGGCGTGCTCATCAACGGTGAGGGGGCAACAGTATCCAATACGGGTGATGtt < 2:11389/139‑1 (MQ=255) gCGGTGTGCATGGTGTTGAGAACATTGGTGACAACGGCACGGTTaa > 1:73213/1‑46 (MQ=255) gCGGTGTGCATGGTGTTGAGAACATTGGTGACAACGGCACGGTTaa < 2:73213/46‑1 (MQ=255) gCGGTGTGCATGGTGTTGAGAACATTGGTGACAACGGCACGGTTAACAACAAAGGAGATATTGTTGTTTCCGATACTGGATCGATTGGCGTGCTCATCAACGGTGAGGGGGCAACAGTATCCAATACGGGTGATGTTaa > 2:231438/1‑139 (MQ=255) | TCAACGGTAACGACGCCACCGCGAATAACAGTGGAAAAACCACTGTTGATGGAAAAGATTCCACGGGTACCAAAATCGCGGGCAATATCGGCATTGTAAATCTGGATGGTAGCCTGACTGTTACAGGCGGTGCGCATGGTGTTGAGAACATTGGTGACAACGGCACGGTTAACAACAAAGGAGATATTGTTGTTTCCGATACTGGATCGATTGGCGTGCTCATCAACGGTGAGGGGGCAACAGTATCCAATACGGGTGATGTTAA > NC_000913/1466929‑1467193 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |