Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,629,489	G→A	G475G (GGC→GGT)	ydfI ←	putative NAD‑dependent D‑mannonate oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,629,489	0	G	A	100.0%	60.3 / NA	18	G475G (GGC→GGT)	ydfI	putative NAD‑dependent D‑mannonate oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/10);  total (8/10)

TGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAATCATCACCAAAGATCGCCTTAATCGCCAGCAATGACTGGACGCGCGCTTTCCCTTCGGCACT  >  NC_000913/1629370‑1629617                                                                                                                        |                                                                                                                                 tgAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCACCATGCGCTAATAAAGACa                                                                                                               >  1:445079/1‑139 (MQ=255)       gaAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCACCATGCGCTAATAAAGACAAGTACg                                                                                                         >  2:310789/1‑139 (MQ=255)        aaaCTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCACCATGCGCTAATAAAGACAAGTACGt                                                                                                        >  2:462942/1‑139 (MQ=255)                accacTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCACCATGCGCTAATAAAGACAAGTACGTTTCCGTCa                                                                                                <  2:103488/139‑1 (MQ=255)                       gACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCACCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGc                                                                                         <  1:36797/139‑1 (MQ=255)                       gACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCACCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGc                                                                                         <  2:186338/139‑1 (MQ=255)                                      ggCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCACCATGCGCtaata                                                                                                                     <  1:293555/96‑1 (MQ=255)                                      ggCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCACCATGCGCtaata                                                                                                                     >  2:293555/1‑96 (MQ=255)                                        cATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCACCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGt                                                                        <  1:310789/139‑1 (MQ=255)                                                aaGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCACCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCaaa                                                                >  2:40673/1‑139 (MQ=255)                                                  gCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCACCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAatc                                                              >  1:68283/1‑139 (MQ=255)                                                                                       cTTCACGGAATATTTTGCCACGGTCGCTTTCGCACCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAATCATCACCAAAGATCGCCTTAATCGCCAGCAATGACTgg                         >  2:58300/1‑139 (MQ=255)                                                                                                         cACGGTCGCTTTCGCACCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTaaa                                                                                   <  1:7346/63‑1 (MQ=255)                                                                                                         cACGGTCGCTTTCGCACCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTaaa                                                                                   >  2:7346/1‑63 (MQ=255)                                                                                                            ggTCGCTTTCGCACCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAATCATCACCAAAGATCGCCTTAATCGCCAGCAATGACTGGACGCGCGCTTTCCCTTCGGCa    <  2:201289/139‑1 (MQ=255)                                                                                                            ggTCGCTTTCGCACCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAATCATCACCAAAGATCGCCTTAATCGCCAGCAATGACTGGACGCGCGCTTTCCCTTCGGCa    <  2:445079/139‑1 (MQ=255)                                                                                                            ggTCGCTTTCGCACCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAATCATCACCAAAGATCGCCTTAATCGCCAGCAATGACTGGACGCGCGCTTTCCCTTCGGCa    <  1:40673/139‑1 (MQ=255)                                                                                                              tCGCTTTCGCACCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAATCATCACCAAAGATCGCCTTAATCGCCAGCAATGACTGGACGCGCGCTTTCCCTTCGGCact  <  1:58300/139‑1 (MQ=255)                                                                                                                        |                                                                                                                                 TGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAATCATCACCAAAGATCGCCTTAATCGCCAGCAATGACTGGACGCGCGCTTTCCCTTCGGCACT  >  NC_000913/1629370‑1629617

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑