Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 156,412 T→G T158P (ACA→CCA)  yadN ← putative fimbrial‑like adhesin protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913156,4120TG98.6% 272.8 / NA 73T158P (ACA→CCA) yadNputative fimbrial‑like adhesin protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base G (30/42);  minor base A (1/0);  total (31/42)
Fisher's exact test for biased strand distribution p-value = 4.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

CAATCAATTTAACTGGTGAATACTTACTGATAAGTAATGGTGTATGCAGTGTTAGTTTTTACATAACCAGCAGTTGCTGTTTGGTCTGCGACGGCACGAACGTAAGACGCTTTAAAATCATAAACAGCAGATTTTGTCGTTGCATCCAGGGCTTTAGTATACACATCGCCAGGGTTGTTAATTTGAACCTGTTTGATAGTGGAACCATCAATATTGTGTAGAGCGATATTAACGCCGTCAGATGGGTTGTTAATAGACATGTCGTTG  >  NC_000913/156276‑156542
                                                                                                                                        |                                                                                                                                  
caatcaatTTAACTGGTGAATACTTACTGATAAGTAATGGTGTATGCAGTGTTAGTTTTTACATAACCAGCAGTTGCTGTTTGGTCTGCGACGGCACGAACGTAAGACGCTTTAAAATCATAAACAGCAGATTTTGGCg                                                                                                                                  >  2:157135/1‑139 (MQ=255)
caatcaatTTAACTGGTGAATACTTACTGATAAGTAATGGTGTATGCAGTGTTAGTTTTTACATAACCAGCAGTTGCTGTTTGGTCTGCGACGGCACGAACGTAAGACGCTTTAAAATCATAAACAGCAGATTTTGGCg                                                                                                                                  >  1:53006/1‑139 (MQ=255)
   tcaatTTAACTGGTGAATACTTACTGATAAGTAATGGTGTATGCAGTGTTAGTTTTTACATAACCAGCAGTTGCTGTTTGGTCTGCGACGGCACGAACGTAAGACGCTTTAAAATCATAAACAGCAGATTTTGGCGTTg                                                                                                                               >  1:56608/1‑139 (MQ=255)
         tAACTGGTGAATACTTACTGATAAGTAATGGTGTATGCAGTGTTAGTTTTTACATAACCAGCAGTTGCTGTTTGGTCTGCGACGGCACGAACGTAAGACGCTTTAAAATCATAAACAGCAGATTTTGGCGTTGCATCCa                                                                                                                         <  1:109389/139‑1 (MQ=255)
         tAACTGGTGAATACTTACTGATAAGTAATGGTGTATGCAGTGTTAGTTTTTACATAACCAGCAGTTGCTGTTTGGTCTGCGACGGCACGAACGTAAGACGCTTTAAAATCATAAACAGCAGATTTTGGCGTTGCATCCa                                                                                                                         <  1:388760/139‑1 (MQ=255)
         tAACTGGTGAATACTTACTGATAAGTAATGGTGTATGCAGTGTTAGTTTTTACATAACCAGCAGTTGCTGTTTGGTCTGCGACGGCACGAACGTAAGACGCTTTAAAATCATAAACAGCAGATTTTGGCGTTGCATCCa                                                                                                                         <  1:150892/139‑1 (MQ=255)
              ggTGAATACTTACTGATAAGTAATGGTGTATGCAGTGTTAGTTTTTACATAACCAGCAGTTGCTGTTTGGTCTGCGACGGCACGAACGTAAGACGCTTTAAAATCATAAACAGCAGATTTTGGCGTTGCATCCAGGGCt                                                                                                                    <  1:138405/139‑1 (MQ=255)
              ggTGAATACTTACTGATAAGTAATGGTGTATGCAGTGTTAGTTTTTACATAACCAGCAGTTGCTGTTTGGTCTGCGACGGCACGAACGTAAGACGCTTTAAAATCATAAACAGCAGATTTTGGCGTTGCATCCAGGGCt                                                                                                                    <  2:56608/139‑1 (MQ=255)
              ggTGAATACTTACTGATAAGTAATGGTGTATGCAGTGTTAGTTTTTACATAACCAGCAGTTGCTGTTTGGTCTGCGACGGCACGAACGTAAGACGCTTTAAAATCATAAACAGCAGATTTTGGCGTTGCATCCAGGGCt                                                                                                                    <  1:439061/139‑1 (MQ=255)
               gTGAATACTTACTGATAAGTAATGGTGTATGCAGTGTTAGTTTTTACATAACCAGCAGTTGCTGTTTGGTCTGCGACGGCACGAACGTAAGACGCTTTAAAATCATAAACAGCAGATTTTGGCGTTGCATCCAGGGCtt                                                                                                                   >  1:202060/1‑139 (MQ=255)
               gTGAATACTTACTGATAAGTAATGGTGTATGCAGTGTTAGTTTTTACATAACCAGCAGTTGCTGTTTGGTCTGCGACGGCACGAACGTAAGACGCTTTAAAATCATAAACAGCAGATTTTGGCGTTGCATCCAGGGCtt                                                                                                                   <  1:343506/139‑1 (MQ=255)
                    tacttactGATAAGTAATGGTGTATGCAGTGTTAGTTTTTACATAACCAGCAGTTGCTGTTTGGTCTGCGACGGCACGAACGTAAGACGCTTTAAAATCATAAACAGCAGATTTTGGCGTTGCATCCAGGGCTTTAGta                                                                                                              <  2:348448/139‑1 (MQ=255)
                         actGATAAGTAATGGTGTATGCAGTGTTAGTTTTTACATAACCAGCAGTTGTTGTTTGGTCTGCGACGGCACGAACGTAAGACGCTTTAAAATCATAAACAGCAGATTTTGGCGTTGCATCCAGGGCtt                                                                                                                   >  2:319323/1‑129 (MQ=255)
                         actGATAAGTAATGGTGTATGCAGTGTTAGTTTTTACATAACCAGCAGTTGTTGTTTGGTCTGCGACGGCACGAACGTAAGACGCTTTAAAATCATAAACAGCAGATTTTGGCGTTGCATCCAGGGCtt                                                                                                                   <  1:319323/129‑1 (MQ=255)
                         actGATAAGTAATGGTGTATGCAGTGTTAGTTTTTACATAACCAGCAGTTGCTGTTTGGTCTGCGACGGCACGAACGTAAGACGCTTTAAAATCATAAACAGCAGATTTTGGCGTTGCATCCAGGGCTTTAGTATacac                                                                                                         >  2:18053/1‑139 (MQ=255)
                         actGATAAGTAATGGTGTATGCAGTGTTAGTTTTTACATAACCAGCAGTTGCTGTTTGGTCTGCGACGGCACGAACGTAAGACGCTTTAAAATCATAAACAGCAGATTTTGGCGTTGCATCCAGGGCTTTAGTATacac                                                                                                         >  2:279603/1‑139 (MQ=255)
                         actGATAAGTAATGGTGTATGCAGTGTTAGTTTTTACATAACCAGCAGTTGCTGTTTGGTCTGCGACGGCACGAACGTAAGACGCTTTAAAATCATAAACAGCAGATTTTGGCGTTGCATCCAGGGCTTTAGTATacac                                                                                                         >  1:243008/1‑139 (MQ=255)
                         actGATAAGTAATGGTGTATGCAGTGTTAGTTTTTACATAACCAGCAGTTGCTGTTTGGTCTGCGACGGCACGAACGTAAGACGCTTTAAAATCATAAACAGCAGATTTTGACGTTGCATCCAGGGCTTTAGTATacac                                                                                                         >  1:295117/1‑139 (MQ=255)
                             aTAAGTAATGGTGTATGCAGTGTTAGTTTTTACATAACCAGCAGTTGCTGTTTGGTCTGCGACGGCACGAACGTAAGACGCTTTAAAATCATAAACAGCAGATTTTGGCGTTGCATCCAGGGCTTTAGTATACACATCg                                                                                                     >  2:464445/1‑139 (MQ=255)
                                aGTAATGGTGTATGCAGTGTTAGTTTTTACATAACCAGCAGTTGCTGTTTGGTCTGCGACGGCACGAACGTAAGACGCTTTAAAATCATAAACAGCAGATTTTGGCGTTGCATCCAGGGCTTTAGTATACACATCGCCa                                                                                                  <  2:189311/139‑1 (MQ=255)
                                     tGGTGTATGCAGTGTTAGTTTTTACATAACCAGCAGTTGCTGTTTGGTCTGCGACGGCACGAACGTAAGACGCTTTAAAATCATAAACAGCAGATTTTGGCGTTGCATCCAGGGCTTTAGTATACACATCGCCAGGgtt                                                                                             >  2:311368/1‑139 (MQ=255)
                                         gtATGCAGTGTTAGTTTTTACATAACCAGCAGTTGCTGTTTGGTCTGCGACGGCACGAACGTAAGACGCTTTAAAATCATAAACAGCAGATTTTGGCGTTGCATCCAGGGCt                                                                                                                    <  1:502621/112‑1 (MQ=255)
                                         gtATGCAGTGTTAGTTTTTACATAACCAGCAGTTGCTGTTTGGTCTGCGACGGCACGAACGTAAGACGCTTTAAAATCATAAACAGCAGATTTTGGCGTTGCATCCAGGGCt                                                                                                                    >  2:502621/1‑112 (MQ=255)
                                            tGCAGTGTTAGTTTTTACATAACCAGCAGTTGCTGTTTGGTCTGCGACGGCACGAACGTAAGACGCTTTAAAATCATAAACAGCAGATTTTGGCGTTGCATCCAGGGCTTTAGTATACACATCGCCAGGGTTGTTAAtt                                                                                      >  1:274997/1‑139 (MQ=255)
                                            tGCAGTGTTAGTTTTTACATAACCAGCAGTTGCTGTTTGGTCTGCGACGGCACGAACGTAAGACGCTTTAAAATCATAAACAGCAGATTTTGGCGTTGCATCCAGGGCTTTAGTATACACATCGCCAGGGTTGTTAAtt                                                                                      >  1:482459/1‑139 (MQ=255)
                                              cAGTGTTAGTTTTTACATAACCAGCAGTTGCTGTTTGGTCTGCGACGGCACGAACGTAAGACGCTTTAAAATCATAAACAGCAGATTTTGGCGTTGCATCCAGGGCTTTAGTATACACATCGCCAGGGTTGTTAATTTg                                                                                    <  1:493413/139‑1 (MQ=255)
                                               aGTGTTAGTTTTTACATAACCAGCAGTTGCTGTTTGGTCTGCGACGGCACGAACGTAAGACGCTTTAAAATCATAAACAGCAGATTTTGGCGTTGCATCCAGGGCt                                                                                                                    <  2:19695/106‑1 (MQ=255)
                                               aGTGTTAGTTTTTACATAACCAGCAGTTGCTGTTTGGTCTGCGACGGCACGAACGTAAGACGCTTTAAAATCATAAACAGCAGATTTTGGCGTTGCATCCAGGGCt                                                                                                                    >  1:19695/1‑106 (MQ=255)
                                               aGTGTTAGTTTTTACATAACCAGCAGTTGCTGTTTGGTCTGCGACGGCACGAACGTAAGACGCTTTAAAATCATAAACAGCAGATTTTGGCGTTGCATCCAGGGCTTTAGTATACACATCGCCAGGGTTGTTAATTTGa                                                                                   <  2:274997/139‑1 (MQ=255)
                                                 tgtTAGTTTTTACATAACCAGCAGTTGCTGTTTGGTCTGCGACGGCACGAACGTAAGACGCTTTAAAATCATAAACAGCAGATTTTGGCGTTGCATCCAGGGCt                                                                                                                    <  1:338861/104‑1 (MQ=255)
                                                 tgtTAGTTTTTACATAACCAGCAGTTGCTGTTTGGTCTGCGACGGCACGAACGTAAGACGCTTTAAAATCATAAACAGCAGATTTTGGCGTTGCATCCAGGGCt                                                                                                                    >  2:338861/1‑104 (MQ=255)
                                                 tgtTAGTTTTTACATAACCAGCAGTTGCTGTTTGGTCTGCGACGGCACGAACGTAAGACGCTTTAAAATCATAAACAGCAGATTTTGGCGTTGCATCCAGGGCTTTAGTATACACATCGCCAGGGTTGTTAATTTGAAc                                                                                 <  2:295117/139‑1 (MQ=255)
                                                 tgtTAGTTTTTACATAACCAGCAGTTGCTGTTTGGTCTGCGACGGCACGAACGTAAGACGCTTTAAAATCATAAACAGCAGATTTTGGCGTTGCATCCAGGGCTTTAGTATACACATCGCCAGGGTTGTTAATTTGAAc                                                                                 >  1:100884/1‑139 (MQ=255)
                                                 tgtTAGTTTTTACATAACCAGCAGTTGCTGTTTGGTCTGCGACGGCACGAACGTAAGACGCTTTAAAATCATAAACAGCAGATTTTGGCGTTGCATCCAGGGCTTTAGTATACACATCGCCAGGGTTGTTAATTTGAAc                                                                                 >  1:448863/1‑139 (MQ=255)
                                                 tgtTAGTTTTTACATAACCAGCAGTTGCTGTTTGGTCTGCGACGGCACGAACGTAAGACGCTTTAAAATCATAAACAGCAGATTTTGGCGTTGCATCCAGGGCTTTAGTATACACATCGCCAGGGTTGTTAATTTGAAc                                                                                 >  2:445323/1‑139 (MQ=255)
                                                  gtTAGTTTTTACATAACCAGCAGTTGCTGTTTGGTCTGCGACGGCACGAACGTAAGACGCTTTAAAATCATAAACAGCAGATTTTGGCGTTGCATCCAGGGCt                                                                                                                    <  1:406557/103‑1 (MQ=255)
                                                  gtTAGTTTTTACATAACCAGCAGTTGCTGTTTGGTCTGCGACGGCACGAACGTAAGACGCTTTAAAATCATAAACAGCAGATTTTGGCGTTGCATCCAGGGCt                                                                                                                    >  2:406557/1‑103 (MQ=255)
                                                  gtTAGTTTTTACATAACCAGCAGTTGCTGTTTGGTCTGCGACGGCACGAACGTAAGACGCTTTAAAATCATAAACAGCAGATTTTGGCGTTGCATCCAGGGCt                                                                                                                    >  2:84431/1‑103 (MQ=255)
                                                  gtTAGTTTTTACATAACCAGCAGTTGCTGTTTGGTCTGCGACGGCACGAACGTAAGACGCTTTAAAATCATAAACAGCAGATTTTGGCGTTGCATCCAGGGCt                                                                                                                    <  1:84431/103‑1 (MQ=255)
                                                  gtTAGTTTTTACATAACCAGCAGTTGCTGTTTGGTCTGCGACGGCACGAACGTAAGACGCTTTAAAATCATAAACAGCAGATTTTGGCGTTGCATCCAGGGCTTTAGTATACACATCGCCAGGGTTGTTAATTTGa                                                                                   <  1:33242/136‑1 (MQ=255)
                                                  gtTAGTTTTTACATAACCAGCAGTTGCTGTTTGGTCTGCGACGGCACGAACGTAAGACGCTTTAAAATCATAAACAGCAGATTTTGGCGTTGCATCCAGGGCTTTAGTATACACATCGCCAGGGTTGTTAATTTGa                                                                                   >  2:33242/1‑136 (MQ=255)
                                                  gtTAGTTTTTACATAACCAGCAGTTGCTGTTTGGTCTGCGACGGCACGAACGTAAGACGCTTTAAAATCATAAACAGCAGATTTTGGCGTTGCATCCAGGGCTTTAGTATACACATCGCCAGGGTTGTTAATTTGAAcc                                                                                >  2:92798/1‑139 (MQ=255)
                                                    tAGTTTTTACATAACCAGCAGTTGCTGTTTGGTCTGCGACGGCACGAACGTAAGACGCTTTAAAATCATAAACAGCAGATTTTGGCGTTGCATCCAGGGCTTTAGTATACACATCGCCAGGGTTGTTAATTTGAACCTg                                                                              <  2:482459/139‑1 (MQ=255)
                                                      gTTTTTACATAACCAGCAGTTGCTGTTTGGTCTGCGACGGCACGAACGTAAGACGCTTTAAAATCATAAACAGCAGATTTTGGCGTTGCATCCAGGGCTTTAGTATACACATCGCCAGGGTTGTTAATTTGAACCTGtt                                                                            <  1:445323/139‑1 (MQ=255)
                                                         tttACATAACCAGCAGTTGCTGTTTGGTCTGCGACGGCACGAACGTAAGACGCTTTAAAATCATAAACAGCAGATTTTGGCGTTGCATCCAGGGCTTTAGTGTACACATCGCCAGGGTTGTTAATTTGAACCTGTTTGa                                                                         >  2:125216/1‑139 (MQ=255)
                                                         tttACATAACCAGCAGTTGCTGTTTGGTCTGCGACGGCACGAACGTAAGACGCTTTAAAATCATAAACAGCAGATTTTGGCGTTGCATCCAGGGCTTTAGTATACACATCGCCAGGGTTGTTAATTTGAACCTGTTTGa                                                                         >  1:181263/1‑139 (MQ=255)
                                                                aaCCAGCAGTTGCTGTTTGGTCTGCGACGGCACGAACGTAAGACGCTTTAAAATCATAAACAGCAGATTTTGGCGTTGCATCCAGGGCt                                                                                                                    >  1:163504/1‑89 (MQ=255)
                                                                aaCCAGCAGTTGCTGTTTGGTCTGCGACGGCACGAACGTAAGACGCTTTAAAATCATAAACAGCAGATTTTGGCGTTGCATCCAGGGCt                                                                                                                    <  2:163504/89‑1 (MQ=255)
                                                                aaCCAGCAGTTGCTGTTTGGTCTGCGACGGCACGAACGTAAGACGCTTTAAAATCATAAACAGCAGATTTTGGCGTTGCATCCAGGGCTTTAGTATACACATCGCCAGGGTTGTTAATTTGAACCTGTTTGATAGTGGa                                                                  <  2:243008/139‑1 (MQ=255)
                                                                aaCCAGCAGTTGCTGTTTGGTCTGCGACGGCACGAACGTAAGACGCTTTAAAATCATAAACAGCAGATTTTGGCGTTGCATCCAGGGCTTTAGTATACACATCGCCAGGGTTGTTAATTTGAACCTGTTTGATAGTGGa                                                                  <  2:454641/139‑1 (MQ=255)
                                                                aaCCAGCAGTTGCTGTTTGGTCTGCGACGGCACGAACGTAAGACGCTTTAAAATCATAAACAGCAGATTTTGGCGTTGCATCCAGGGCTTTAGTATACACATCGCCAGGGTTGTTAATTTGAACCTGTTTGATAGTGGa                                                                  <  1:464445/139‑1 (MQ=255)
                                                                  ccAGCAGTTGCTGTTTGGTCTGCGACGGCACGAACGTAAGACGCTTTAAAATCATAAACAGCAGATTTTGGCGTTGCATCCAGGGCtt                                                                                                                   >  2:344532/1‑88 (MQ=255)
                                                                  ccAGCAGTTGCTGTTTGGTCTGCGACGGCACGAACGTAAGACGCTTTAAAATCATAAACAGCAGATTTTGGCGTTGCATCCAGGGCtt                                                                                                                   <  1:344532/88‑1 (MQ=255)
                                                                      cagTTGCTGTTTGGTCTGCGACGGCACGAACGTAAGACGCTTTAAAATCATAAACAGCAGATTTTGGCGTTGCATCCAGGGCTTTAGTATACACATCGCCAGGGTTGTTAATTTGAACCTGTTTGATAGTGGAACCATc                                                            >  1:239507/1‑139 (MQ=255)
                                                                       agTTGCTGTTTGGTCTGCGACGGCACGAACGTAAGACGCTTTAAAATCATAAACAGCAGATTTTGGCGTTGCATCCAGGGCTTTAGTATACACATCGCCAGGGTTGTTAATTTGAACCTGTTTGATAGTGGGACCATCa                                                           <  2:181263/139‑1 (MQ=255)
                                                                       agTTGCTGTTTGGTCTGCGACGGCACGAACGTAAGACGCTTTAAAATCATAAACAGCAGATTTTGGCGTTGCATCCAGGGCTTTAGTATACACATCGCCAGGGTTGTTAATTTGAACCTGTTTGATAGTGGAACCATCa                                                           <  2:121981/139‑1 (MQ=255)
                                                                       agTTGCTGTTTGGTCTGCGACGGCACGAACGTAAGACGCTTTAAAATCATAAACAGCAGATTTTGGCGTTGCATCCAGGGCTTTAGTATACACATCGCCAGGGTTGTTAATTTGAACCTGTTTGATAGTGGAACCATCa                                                           <  2:448863/139‑1 (MQ=255)
                                                                               tttGGTCTGCGACGGCACGAACGTAAGACGCTTTAAAATCATAAACAGCAGATTTTGGCGTTGCATCCAGGGCTTTAGTGTACACATCGCCAGGGTTGTTAATTTGAACCTGTTTGATAGTGGAACCATCAATATtgtg                                                   <  1:125216/139‑1 (MQ=255)
                                                                                                cGAACGTAAGACGCTTTAAAATCATAAACAGCAGATTTTGGCGTTGCATCCAGGGCTTTAGTATACACATCGCCAGGGTTGTTAATTTGAACCTGTTTGATAGTGGAACCATCAATATTGTGTAGAGCGATATTAACGc                                  <  2:53006/139‑1 (MQ=255)
                                                                                                 gAACGTAAGACGCTTTAAAATCATAAACAGCAGATTTTGGCGTTGCATCCAGGGCTTTAGTATACACATCGCCAGGGTTGTTAATTTGAACCTGTTTGATAGTGGAACCATCAATATTGTGTAGAGCGATATTAACGcc                                 <  1:279603/139‑1 (MQ=255)
                                                                                                 gAACGTAAGACGCTTTAAAATCATAAACAGCAGATTTTGGCGTTGCATCCAGGGCTTTAGTATACACATCGCCAGGGTTGTTAATTTGAACCTGTTTGATAGTGGAACCATCAATATTGTGTAGAGCGATATTAACGcc                                 <  1:35702/139‑1 (MQ=255)
                                                                                                       aaGACGCTTTAAAATCATAAACAGCAGATTTTGGCGTTGCATCCAGGGCTTTAGTATACACATCGCCAGGGTTGTTAATTTGAACCTGTTTGATAGTGGAACCATCAATATTGTGTAGAGCGATATTAACGCCGTCAGa                           <  1:18053/139‑1 (MQ=255)
                                                                                                        aGACGCTTTAAAATCATAAACAGCAGATTTTGGCGTTGCATCCAGGGCTTTAGTATACACATCGCCAGGGTTGTTAATTTGAACCTGTTTGATAGTGGAACCATCAATATTGTGTAGAGCGATATTAACGCCGTCAGAt                          <  1:446235/139‑1 (MQ=255)
                                                                                                           cGCTTTAAAATCATAAACAGCAGATTTTGGCGTTGCATCCAGGGCTTTAGTATACACATCGCCAGGGTTGTTAATTTGAACCTGTTTGATAGTGGAACCATCAATATTGTGTAGAGCGATATTAACGCCGTCAGATggg                       <  2:100884/139‑1 (MQ=255)
                                                                                                            gCTTTAAAATCATAAACAGCAGATTTTGGCGTTGCATCCAGGGCTTTAGTATACACATCGCCAGGGTTGTTAATTTGAACCTGTTTGATAGTGGAACCATCGATATTGTGTAGAGCGATATTAACACCTTCAGATGGgt                      <  1:343510/139‑1 (MQ=255)
                                                                                                            gCTTTAAAATCATAAACAGCAGATTTTGGCGTTGCATCCAGGGCTTTAGTATACACATCGCCAGGGTTGTTAATTTGAACCTGTTTGATAGTGGAACCATCAATATTGTGTAGAGCGATATTAACGCCGTCAGATGGgt                      >  2:237977/1‑139 (MQ=255)
                                                                                                            gCTTTAAAATCATAAACAGCAGATTTTGGCGTTGCATCCAGGGCTTTAGTATACACATCGCCAGGGTTGTTAATTTGAACCTGTTTGATAGTGGAACCATCAATATTGTGTAGAGCGATATTAACGCCGTCAGATGGgt                      <  2:202060/139‑1 (MQ=255)
                                                                                                             cTTTAAAATCATAAACAGCAGATTTTGGCGTTGCATCCAGGGCTTTAGTATACACATCGCCAGGGTTGTTAATTTGAACCTGTTTGATAGTGGAACCATCAATATTGTGTAGAGCGATATTAACGCCGTCAGATGGgtt                     >  1:496052/1‑139 (MQ=255)
                                                                                                                tAAAATCATAAACAGCAGATTTTGGCGTTGCATCCAGGGCTTTAGTATACACATCGCCAGGGTTGTTAATTTGAACCTGTTTGATAGTGGAACCATCAATATTGTGTAGAGCGATATTAACGCCGTCAGATGGgttgtt                  <  1:229480/139‑1 (MQ=255)
                                                                                                                   aaTCATAAACAGCAGATTTTGGCGTTGCATCCAGGGCTTTAGTATACACATCGCCAGGGTTGTTAATTTGAACCTGTTTGATAGTGGAACCATCAATATTGTGTAGAGCGATATTAACGCCGTCAGATGGGTTGTTAAt               <  1:157135/139‑1 (MQ=255)
                                                                                                                               cagATTTTGGCGTTGCATCCAGGGCTTTAGTATACACATCGCCAGGGTTGTTAATTTGAACCTGTTTGATAGTGGAACCATCAATATTGTGTAGAGCGATATTAACGCCGTCAGAt                          >  2:42539/1‑116 (MQ=255)
                                                                                                                               cagATTTTGGCGTTGCATCCAGGGCTTTAGTATACACATCGCCAGGGTTGTTAATTTGAACCTGTTTGATAGTGGAACCATCAATATTGTGTAGAGCGATATTAACGCCGTCAGAt                          <  1:42539/116‑1 (MQ=255)
                                                                                                                                agATTTTGGCGTTGCATCCAGGGCTTTAGTATACACATCGCCAGGGTTGTTAATTTGAACCTGTTTGATAGTGGAACCATCAATATTGTGTAGAGCGATATTAACGCCGTCAGATGGGTTGTTAATAGACATGTCgttg  <  2:290510/139‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                  
CAATCAATTTAACTGGTGAATACTTACTGATAAGTAATGGTGTATGCAGTGTTAGTTTTTACATAACCAGCAGTTGCTGTTTGGTCTGCGACGGCACGAACGTAAGACGCTTTAAAATCATAAACAGCAGATTTTGTCGTTGCATCCAGGGCTTTAGTATACACATCGCCAGGGTTGTTAATTTGAACCTGTTTGATAGTGGAACCATCAATATTGTGTAGAGCGATATTAACGCCGTCAGATGGGTTGTTAATAGACATGTCGTTG  >  NC_000913/156276‑156542

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: