Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,827,934	G→A	A109V (GCC→GTC)	astB ←	succinylarginine dihydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,827,934	0	G	A	100.0%	40.8 / NA	13	A109V (GCC→GTC)	astB	succinylarginine dihydrolase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (5/8);  total (5/8)

TTCAGTGACGGGCGCTTCCAGCGAACGGTGAAATTTATTGTTCAGGTTGGCAACGGTGAGATGCACTTTGCCATCCAGCGTATCGGCAGATGGCGCGATCGTTGCCGCATTGGCTACCCACATTGGCGAAGCGGAACTGACGCTGGAAAGCCAGTGCGGTGCCTGGCGTGCAACTTTTTCCAGTACCTGCTCATCGCTACCACTGAATCCCAACTGACG  >  NC_000913/1827822‑1828040                                                                                                                 |                                                                                                           ttCAGTGACGGGCGCTTCCAGCGAACGGTGAAATTTATTGTTCAGGTTGGCAACGGTGAGATGCACTTTGCCATCCAGCGTATCGGCAGATGGCGCGATCGTTGCCGCATTGACTACCCACATTGGCGAAGCGGAACTg                                                                                  <  1:135619/139‑1 (MQ=255)         cGGGCGCTTCCAGCGAACGGTGAAATTTATTGTTCAGGTTGGCAACGGTGAGATGCACTTTGCCATCCAGCGTATCGGCAGATGGCGCGATCGTTGCCGCATTGACTACCCACATTGGCGAAGCGGAACTGACGCTGGa                                                                          <  2:452118/139‑1 (MQ=255)              gcTTCCAGCGAACGGTGAAATTTATTGTTCAGGTTGGCAACGGTGAGATGCACTTTGCCATCCAGCGTATCGGCAGATGGCGCGATCGTTGCCGCATTGACTACCCACATTGGCGAAGCGGAACTGACGCTGGAAAGcc                                                                     >  2:40988/1‑139 (MQ=255)                                         ttCAGGTTGGCAACGGTGAGATGCACTTTGCCATCCAGCGTATCGGCAGATGGCGCGATCGTTGCCGCATTGACTACCCACATTGGCGAAGCGGAACTGACGCTGGAAAGCCAGTGCGGTGCCTGGCGTGCAACttttt                                          <  1:326463/139‑1 (MQ=255)                                                           agaTGCACTTTGCCATCCAGCGTATCGGCAGATGGCGCGATCGTTGCCGCATTGACTACCCACATTGGCGAAGCGGAACTGACGCTGGAAAGCCAGTGCGGTGCCTGGCGTGCAACt                                              <  1:124424/117‑1 (MQ=255)                                                           agaTGCACTTTGCCATCCAGCGTATCGGCAGATGGCGCGATCGTTGCCGCATTGACTACCCACATTGGCGAAGCGGAACTGACGCTGGAAAGCCAGTGCGGTGCCTGGCGTGCAACt                                              >  2:124424/1‑117 (MQ=255)                                                                cACTTTGCCATCCAGCGTATCGGCAGATGGCGCGATCGTTGCCGCATTGACTACCCACATTGGCGAAGCGGAACTGACGCTGGAAAGCCAGTGCGGTGCCTGGCGTGCAACTTTTTCCAGTACCTGCTCAt                           <  1:96380/131‑1 (MQ=255)                                                                cACTTTGCCATCCAGCGTATCGGCAGATGGCGCGATCGTTGCCGCATTGACTACCCACATTGGCGAAGCGGAACTGACGCTGGAAAGCCAGTGCGGTGCCTGGCGTGCAACTTTTTCCAGTACCTGCTCAt                           >  2:96380/1‑131 (MQ=255)                                                                  cTTTGCCATCCAGCGTATCGGCAGATGGCGCGATCGTTGCCGCATTGACTACCCACATTGGCGAAGCGGAACTGACGCTGGAAAGCCAGTGCGGTGCCTGGCGTGCAACTTTTTCCAGTACCTGCTCATCGCTACCACt                 <  1:40988/139‑1 (MQ=255)                                                                     tGCCATCCAGCGTATCGGCAGATGGCGCGATCGTTGCCGCATTGACTACCCACATTGGCGAAGCGGAACTGACGCTGGAAAGCCAGTg                                                                 <  1:216768/88‑1 (MQ=255)                                                                     tGCCATCCAGCGTATCGGCAGATGGCGCGATCGTTGCCGCATTGACTACCCACATTGGCGAAGCGGAACTGACGCTGGAAAGCCAGTg                                                                 >  2:216768/1‑88 (MQ=255)                                                                               cGTATCGGCAGATGGCGCGATCGTTGCCGCATTGACTACCCACATTGCCGAAGCGGAACTGACGCTGGAAAGCCAGTGCGGTGCCTGGCGTGCAACTTTTTCCAGTACCTGCTCATCGCTTCCACTGAATCCCAACTGa    >  1:434526/1‑139 (MQ=255)                                                                                 tATCGGCAGATGGCGCGATCGTTGCCGCATTGACTACCCACATTGGCGAAGCGGAACTGACGCTGGAAAGCCAGTGCGGTGCCTGGCGTGCAACTTTTTCCAGTACCTGCTCATCGCTACCACTGAATCCCAACTGACg  <  2:429302/139‑1 (MQ=255)                                                                                                                 |                                                                                                           TTCAGTGACGGGCGCTTCCAGCGAACGGTGAAATTTATTGTTCAGGTTGGCAACGGTGAGATGCACTTTGCCATCCAGCGTATCGGCAGATGGCGCGATCGTTGCCGCATTGGCTACCCACATTGGCGAAGCGGAACTGACGCTGGAAAGCCAGTGCGGTGCCTGGCGTGCAACTTTTTCCAGTACCTGCTCATCGCTACCACTGAATCCCAACTGACG  >  NC_000913/1827822‑1828040

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑