Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,036,909	T→C	Q415R (CAG→CGG)	yedV ←	putative sensory kinase in two‑component regulatory system with YedW

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,036,909	0	T	C	100.0%	106.2 / NA	29	Q415R (CAG→CGG)	yedV	putative sensory kinase in two‑component regulatory system with YedW
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/12);  total (17/12)

GCGGTAACGTAATCCGGAACACATTATGCTTATTGAGATAGTGATACGTAGCACTTCCCCCATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCTGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGA  >  NC_000913/2036805‑2037043                                                                                                         |                                                                                                                                       gCGGTAACGTAATCCGGAACACATTATGCTTATTGAGATAGTGATACGTAGCACTTCCCCCATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCa                                                                                                      <  1:230708/139‑1 (MQ=255)                 gAACACATTATGCTTATTGAGATAGTGATACGTAGCACTTCCCCCATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGt                                                                                      >  1:470189/1‑139 (MQ=255)                  aaCACATTATGCTTATTGAGGTAGTGATACGTAGCACTTCCCCCATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGtt                                                                                     >  2:47486/1‑139 (MQ=255)                        ttATGCTTATTGAGATAGTGATACGTAGCACTTCCCCCATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAg                                                                               <  2:470189/139‑1 (MQ=255)                                      aTAGTGATACGTAGCACTTCCCCCATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTc                                                                 >  1:26122/1‑139 (MQ=255)                                        aGTGATACGTAGCACTTCCCCCATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGt                                                               >  1:326430/1‑139 (MQ=255)                                               cGTAGCACTTCCCCCATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAggg                                                        >  2:74723/1‑139 (MQ=255)                                                  aGCACTTCCCCCATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTg                                                     >  2:189395/1‑139 (MQ=255)                                                    cACTTCCCCCATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGc                                                   >  2:423338/1‑139 (MQ=255)                                                     aCTTCCCCCATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCg                                                  <  1:423338/139‑1 (MQ=255)                                                         cccccATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGatat                                              >  2:73448/1‑139 (MQ=255)                                                         cccccATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGatat                                              >  2:477397/1‑139 (MQ=255)                                                         cccccATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGatat                                              <  1:270389/139‑1 (MQ=255)                                                          caccATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTGCGGAAAGACGCGAATTATCTCCCCGCCAACATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGTTGGCGATATc                                             <  2:277443/137‑1 (MQ=255)                                                                   aTTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAg                                    >  2:207670/1‑139 (MQ=255)                                                                    ttCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAag                                                                                         >  1:368492/1‑85 (MQ=255)                                                                    ttCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAag                                                                                         <  2:368492/85‑1 (MQ=255)                                                                    ttCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGa                                   >  2:480412/1‑139 (MQ=255)                                                                    ttCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGa                                   <  1:207670/139‑1 (MQ=255)                                                                    ttCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACGGAATGACGCGAATTAACTCTCCGCCAAAATCTACGGAAGAGTTTTTGAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGa                                   >  1:427571/1‑139 (MQ=255)                                                                            tCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCTATATCAATATTAAGATAGCTGt                            <  1:32599/138‑1 (MQ=255)                                                                            tCGCTTTGACTAAAGAAAGGCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCTATATCAATATTAAGATAGCTGt                            >  2:32599/1‑138 (MQ=255)                                                                                     cTAAAGAAAGGCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAg                  <  1:189395/139‑1 (MQ=255)                                                                                         agaaagGCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGaaaa              <  2:26122/139‑1 (MQ=255)                                                                                              ggCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATc                                             >  2:165600/1‑103 (MQ=255)                                                                                              ggCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATc                                             <  1:165600/103‑1 (MQ=255)                                                                                              ggCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGaaaa              <  2:168529/134‑1 (MQ=255)                                                                                              ggCCTAGTCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGaaaa              >  1:168529/1‑134 (MQ=255)                                                                                                     tCCCCGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGa  >  2:1548/1‑139 (MQ=255)                                                                                                         |                                                                                                                                       GCGGTAACGTAATCCGGAACACATTATGCTTATTGAGATAGTGATACGTAGCACTTCCCCCATGCAATTCGGCAATCGCTTTGACTAAAGAAAGGCCTAGTCCCTGACCTACGGAATGACGCGAATTATCTCCCCGCCAAAATCTACGGAAGAGTTTTTCAGGCTCATTAATTTTCGTTCCAGGGCTGGCGATATCAATATTAAGATAGCTGTTGGTATCAAGAAAACTGGTTATATGA  >  NC_000913/2036805‑2037043

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑