Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,040,142	T→C	V222A (GTA→GCA)	yedY →	membrane‑anchored, periplasmic TMAO, DMSO reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,040,142	0	T	C	100.0%	50.3 / NA	15	V222A (GTA→GCA)	yedY	membrane‑anchored, periplasmic TMAO, DMSO reductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (6/9);  total (6/9)

GTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGACCGTAGGTGTTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGT  >  NC_000913/2040006‑2040238                                                                                                                                         |                                                                                                 gTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTTATGACCGCAg                                                                                                <  1:102177/139‑1 (MQ=255)                          cGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGACCGCAGGTGTTTATGGCAAGGCGTTACCGcc                                                                       <  1:339942/139‑1 (MQ=255)                          cGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGACCGCAGGTGTTTATGGCAAGGCGTTACCGcc                                                                       >  2:339942/1‑139 (MQ=255)                                       aggccagCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGACCGCAGGTGTTTATGGCAAGGCGTTACCGCCACAAAATGgcgcg                                                          >  2:10036/1‑139 (MQ=255)                                            agcagGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGACCGCAGGTGTTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGt                                                     >  2:391810/1‑139 (MQ=255)                                                            cggcggTGGGCTGAAATATCCTCATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGACCGCAGGTGTTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGATTGATTGTGCCg                                     <  2:149190/139‑1 (MQ=255)                                                                    ggCTGAATTATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGACCGCAGGTGTTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAata                             <  2:36364/139‑1 (MQ=255)                                                                      cTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGACCGCAGGTGTTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAATATg                           <  2:289135/139‑1 (MQ=255)                                                                             atCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATACATCCGCTCACACTGATGACCGCAGGTGTTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAATATGGCTTTaa                    <  2:333268/139‑1 (MQ=255)                                                                                         aaGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGACCGCAGGTGTTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATc        >  2:201255/1‑139 (MQ=255)                                                                                          aGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGACCGCAGGTGTTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCg       <  1:175668/139‑1 (MQ=255)                                                                                          aGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGACCGCAGGTGTTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCg       >  2:20149/1‑139 (MQ=255)                                                                                           ggATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGACCGCAGGTGTTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGa      <  2:196973/139‑1 (MQ=255)                                                                                               tGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGACCGCAGGTGTTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGt  <  1:10036/139‑1 (MQ=255)                                                                                               tGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGACCGCAGGTGTTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGt  >  1:111092/1‑139 (MQ=255)                                                                                                                                         |                                                                                                 GTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGACCGTAGGTGTTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGT  >  NC_000913/2040006‑2040238

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑