Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,653,260	T→C	V256A (GTG→GCG)	sseA →	3‑mercaptopyruvate sulfurtransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,653,260	0	T	C	100.0%	59.4 / NA	17	V256A (GTG→GCG)	sseA	3‑mercaptopyruvate sulfurtransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (8/9);  total (8/9)

GCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGACAAACCAATTATCGTCAGCTGCGGCTCTGGTGTAACGGCAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTG  >  NC_000913/2653125‑2653395                                                                                                                                        |                                                                                                                                        gCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGACAAACCAATTATCGTCAGCTGCGGCTCTGGTGTAACGGCAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGATGCGcc                                                                                                                                      <  2:392073/139‑1 (MQ=255)                ccGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGACAAACCAATTATCGTCAGCTGCGGCTCTGGTGTAACGGCAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGATGCGCCAAACGTGAAACTGTa                                                                                                                       <  1:469622/139‑1 (MQ=255)                   aTGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGACAAACCAATTATCGTCAGCTGCGGCTCTGGTGTAACGGCAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGATGCGCCAAACGTGAAACTGTacga                                                                                                                    >  1:362450/1‑139 (MQ=255)                      aaCTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGACAAACCAATTATCGTCAGCTGCGGCTCTGGTGTAACGGCAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGATGCGCCAAACGTGAAACTGTACGACgg                                                                                                                 <  2:219969/139‑1 (MQ=255)                             tGCGATATTTTTTGGTCGCGGCGTCAGCTACCACAAACCAATTATCGTCAGCTGCGGCTCTGGTGTAACGGCAGCCGTGGTTTTGTTAGGACTCGCGACGCTGGATGCGCCAAACGTGAAACTGTACGACGGCGCATgg                                                                                                          <  2:172045/139‑1 (MQ=255)                                            tCGCGGCGTCAGCTACGACAAACCAATTATCGTCAGCTGCGGCTCTGGTGTAACAGCAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGATGCGCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGgcgcg                                                                                           <  1:282472/139‑1 (MQ=255)                                                                 aCCAATTATCGTCAGCTGCGGCTCTGGTGTAACGGCAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGATGCGCCAAACGTGAAACTGTACGACGGCGCATgg                                                                                                          <  1:252901/103‑1 (MQ=255)                                                                 aCCAATTATCGTCAGCTGCGGCTCTGGTGTAACGGCAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGATGCGCCAAACGTGAAACTGTACGACGGCGCATgg                                                                                                          >  2:252901/1‑103 (MQ=255)                                                                           gTCAGCTGCGGCTCTGGTGTAACGGCAGCTGTGGTTTTGTGAGCACTCGCGACGCTGGATGCGCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAAt                                                            <  1:390488/139‑1 (MQ=255)                                                                           gTCAGCTGCGGCTCTGGTGTAACGGCAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGATGCGCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAAt                                                            <  1:437791/139‑1 (MQ=255)                                                                              aGCTGCGGCTCTGGTGTAACGGCAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGATGCGCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAg                                                         >  1:332638/1‑139 (MQ=255)                                                                                cTGCGGCTCTGGTGTAACGGCAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGATGCGCCAAACGTGAAACTGTacgac                                                                                                                   >  2:506646/1‑79 (MQ=255)                                                                                cTGCGGCTCTGGTGTAACGGCAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGATGCGCCAAACGTGAAACTGTacgac                                                                                                                   <  1:506646/79‑1 (MQ=255)                                                                                   cGGCTCTGGTGTAACGGCAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGATGCGCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTAttt                                                    >  1:399078/1‑139 (MQ=255)                                                                                   cGGCTCTGGTGTAACGGCAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGATGCGCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTAttt                                                    >  1:16260/1‑139 (MQ=255)                                                                                                      gCCGTGGTTTTGTTAGCACTCGCGACGCTGGATGCGCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAAccg                                 >  2:33327/1‑139 (MQ=255)                                                                                                                                     aTGCGCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTg  >  1:168501/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                                        GCGAACTAAAAACGACCGATGAACTGGATGCGATATTTTTTGGTCGCGGCGTCAGCTACGACAAACCAATTATCGTCAGCTGCGGCTCTGGTGTAACGGCAGCCGTGGTTTTGTTAGCACTCGCGACGCTGGATGTGCCAAACGTGAAACTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTG  >  NC_000913/2653125‑2653395

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑