Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,842,591	T→C	L151L (TTA→TTG)	hycI ←	protease involved in processing C‑terminal end of HycE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,842,591	0	T	C	100.0%	91.0 / NA	25	L151L (TTA→TTG)	hycI	protease involved in processing C‑terminal end of HycE
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/11);  total (14/11)

CCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCTAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCATCTTTAATCGGCTGGGTCATCGGGTAGTAAAAGCCGACGATATCCGGCTG  >  NC_000913/2842478‑2842704                                                                                                                  |                                                                                                                  cccGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATttcctt                                                                                          >  2:452085/1‑139 (MQ=255) cccGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATttcctt                                                                                          <  2:432059/139‑1 (MQ=255)                           tttAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACg                                                                <  1:452085/139‑1 (MQ=255)                                       cATAAGAGCTGGTTCACCCCATCAACAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCa                                                    <  2:331346/139‑1 (MQ=255)                                             agCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCAt                                                   <  1:16633/134‑1 (MQ=255)                                             agCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCAt                                                   <  1:193785/134‑1 (MQ=255)                                             agCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCAt                                                   >  2:193785/1‑134 (MQ=255)                                             agCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCAt                                                   >  2:16633/1‑134 (MQ=255)                                             agCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCATCTTTa                                              <  2:124384/139‑1 (MQ=255)                                                       ccccATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCATCTTTAATCGGCTggg                                    >  1:76831/1‑139 (MQ=255)                                                            tCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCATCTTTAATCGGCTGGGTCATc                               <  2:76831/139‑1 (MQ=255)                                                               aGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCATCTTTAATCGGCTGGGTCATCggg                            <  1:3857/139‑1 (MQ=255)                                                                gAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCATCTTTAATCGGCTGGGTCATCGGgt                           >  1:48928/1‑139 (MQ=255)                                                                      cccTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCATCTTTAATCGGCTGGGTCATCGGGTAGTaaa                     >  2:219120/1‑139 (MQ=255)                                                                      cccTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCATCTTTAATCGGCTGGGTCATCGGGTAGTaaa                     >  1:346793/1‑139 (MQ=255)                                                                           tCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCATCTTTAATCGGCTGGGTCATCGGGTAGTAAAAGCcg                >  2:152658/1‑139 (MQ=255)                                                                               gATTCCTTAATGAAAAACTACTCTTCTTCCACCGCCACCTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCATCTTAAATCGGCTGGGTCATCGGGTAGTAAAAGCcgacga            >  1:341591/1‑139 (MQ=255)                                                                                aTTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACg                                                                <  1:47523/86‑1 (MQ=255)                                                                                aTTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACg                                                                >  2:47523/1‑86 (MQ=255)                                                                                aTTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCATCTTTAATCGGCTGGGTCATCGGGTAGTAAAAGCCGACGat           >  1:5058/1‑139 (MQ=255)                                                                                aTTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCATCTTTAATCGGCTGGGTCATCGGGTAGTAAAAGCCGACGat           >  2:508137/1‑139 (MQ=255)                                                                                aTTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCATCTTTAATCGGCTGGGTCATCGGGTAGTAAAAGCCGACGat           >  2:82989/1‑139 (MQ=255)                                                                                   ccTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCATCTTTAATCGGCTGGGTCATCGGGTAGTAAAAGCCGACGATATc        <  1:219120/139‑1 (MQ=255)                                                                                     ttAATGAAAAACTACTCTTCCTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCATCTTTAATCGGCTGGGTCATCGGGTAGTAAAAGCCGACGATATCCg      >  1:160768/1‑139 (MQ=255)                                                                                         tGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCACATCTTTAATCGGCTGGGTCATCGGGTAGTAAAAGCCGACGATATCCGGCTg  <  2:346793/139‑1 (MQ=255)                                                                                                                  |                                                                                                                  CCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCTAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCATCTTTAATCGGCTGGGTCATCGGGTAGTAAAAGCCGACGATATCCGGCTG  >  NC_000913/2842478‑2842704

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑