Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,920,310	A→G	V146A (GTC→GCC)	gudX ←	glucarate dehydratase‑related protein, substrate unknown

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,920,310	0	A	G	100.0%	66.9 / NA	19	V146A (GTC→GCC)	gudX	glucarate dehydratase‑related protein, substrate unknown
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (10/9);  total (10/9)

CTGTTCATTGCTTTCTGATGCCGCAACTGATACCACTCATGGTTGCCCGGCGTATTTTCCACATAAGGAAGATCGGTTTTGGTCCGATCACCGATATAAAACAGATAACCGAGGACGGTAATAGCCTCGCGTTGCTTGCCTGGCCCTAACAGTTCGCAGACCGGAACATTCAGCGCCTTACCTAGCAGGTCAAGCAAAGCGGCTTCCAGCGCCGCCACGGCGTTAACGCGTAATTCAAAGGTCCAGGCAC  >  NC_000913/2920196‑2920445                                                                                                                   |                                                                                                                                        cTGTTCATTGCTTTCTGATGCCGCAACTGATACCACTCATGGTTGCCCGGCGTATTTTCCACATAAGGAAGATCGGTTTTGGTCCGATCACCGATATAAAACAGATAACCGAGGGCGGTAATAGCCTCGCGttgcttgc                                                                                                                 <  2:340775/139‑1 (MQ=255)   gTTCATTGCTTTCTGATGCCGCAACTGATACCACTCATGGTTGCCCGGCGTATTTTCCACATAAGGAAGATCGGTTTTGGTCCGATCACCGATATAAAACAGATAACCGAGGGCGGTAATAGCCTCGCGTTGCTTGCCt                                                                                                               <  2:69145/139‑1 (MQ=255)                     ccGCAACTGATACCACTCATGGTTGCCCGGCGTATTTTCCACATAAGGAAGATCGGTTTTGGTCCGATCACCGATATAAAACAGATAACCGAGGGCGGTAATAGCCTCGCGTTGCTTGCCTGGCCCTAACAGTTCGCAg                                                                                             >  1:169479/1‑139 (MQ=255)                        cAACTGATACCACTCATGGTTGCCCGGCGTATTTTCCACATAAGGAAGATCGGTTTTGGTCCGATCACCGATATAAAACAGATAACCGAGGGCGGTAATAGCCTCGCGTTGCTTGCCTGGCCCTAACAGTTCGCAGAcc                                                                                          >  2:44221/1‑139 (MQ=255)                                aCCACTCATGGTTGCCCGGCGTATTTTCCACATAAGGAAGATCGGTTTTGGTCCGATCACCGATATAAAACAGATAACCGAGGGCGGTAATAGCCTCGCGTTGCTTGCCTGGCCCTAACAGTTCGCAGACCGGAACAtt                                                                                  <  1:44221/139‑1 (MQ=255)                                         ggTTGCCCGGCGTATTTTCCACATAAGGAAGATCGGTTTTGGTCCGATCACCGATATAAAACAGATAACCGAGGGCGGTAATAGCCTCGCGTTGCTTGCCTGGCCCTAACAGTTCGCAGACCGGAACATTCa                                                                                >  2:503136/1‑132 (MQ=255)                                         ggTTGCCCGGCGTATTTTCCACATAAGGAAGATCGGTTTTGGTCCGATCACCGATATAAAACAGATAACCGAGGGCGGTAATAGCCTCGCGTTGCTTGCCTGGCCCTAACAGTTCGCAGACCGGAACATTCa                                                                                <  1:503136/132‑1 (MQ=255)                                          gTTGCCCGGCGTATTTTCCACATAAGGAAGATCGGTTTTGGTCCGATCACCGATATAAAACAGATAACCGAGGGCGGTAATAGCCTCGCGttgcttgc                                                                                                                 >  1:6558/1‑98 (MQ=255)                                          gTTGCCCGGCGTATTTTCCACATAAGGAAGATCGGTTTTGGTCCGATCACCGATATAAAACAGATAACCGAGGGCGGTAATAGCCTCGCGttgcttgc                                                                                                                 <  2:6558/98‑1 (MQ=255)                                                             acaTAAGGAAGATCGGTTTTGGTCCGATCACCGATATAAAACAGATAACCGAGGGCGGTAATAGCCTCGCGTTGCTTGCCTGGCCCTAACAGTTCGCAGACCGGAACATTCAGCGCCTTACCTAGCAGGTCAAGCAAAg                                                     >  1:327508/1‑139 (MQ=255)                                                               aTAAGGAAGATCGGTTTTGGTCCGATCACCGATATAAAACAGATAACCGAGGGCGGTAATAGCCTCGCGTTGCTTGCCTGGCCCTAACAGTTCGCAGACCGGAACATTCAGCGCCTTACCTAGCAGGTCAAGCAAAGCg                                                   >  1:362690/1‑139 (MQ=255)                                                                        aTCGGTTTTGGTCCGATCACCGATATAAAACAGATAACCGAGGGCGGTAATAGCCTCGCGTTGCTTGCCTGGCCCTAACAGTTCGCAGACCGGAACATTCAGCGCCTTACCTAGCAGGTCAAGCAAAGCGGCTTCCAgc                                          >  2:207537/1‑139 (MQ=255)                                                                                                   aaaCAGATAACCGAGGGCGGTAATAGCCTGGCGTTGCTTGCCTGGCCCTAACAGTTCGCAGACCGGAACATTCAGCGCCTTACCTAGCAGGTCAAGCAAAGCGGCTTCCAGCGCCGCCACGGCGTTAACGCGTAATTCa               <  1:282580/139‑1 (MQ=255)                                                                                                         aTAACCGAGGGCGGTAATAGCCTCGCGTTGCTTGCCTGGCCCTAACAGTTCGCAGACCGGAACATTCAGCGCCTTACCTAGc                                                                  <  1:437454/82‑1 (MQ=255)                                                                                                         aTAACCGAGGGCGGTAATAGCCTCGCGTTGCTTGCCTGGCCCTAACAGTTCGCAGACCGGAACATTCAGCGCCTTACCTAGc                                                                  >  2:437454/1‑82 (MQ=255)                                                                                                         aTAACCGAGGGCGGTAATAGCCTCGCGTTGCTTGCCTGGCCCTAACAGTTCGCAGACCGGAACATTCAGCGCCTTACCTAGCAGGTCAAGCAAAGCGGCTTCCAGCGCCGCCACGGCGTTAACGCGTAATTCAAAGGTc         >  1:169571/1‑139 (MQ=255)                                                                                                             ccGAGGGCGGTAATAGCCTCGCGTTGCTTGCCTGGc                                                                                                            <  2:290908/36‑1 (MQ=255)                                                                                                             ccGAGGGCGGTAATAGCCTCGCGTTGCTTGCCTGGc                                                                                                            >  1:290908/1‑36 (MQ=255)                                                                                                                aGGGCGGTAATAGCCTCGCGTTGCTTGCCTGGCCCTAACAGTTCGCAGACCGGAACATTCAGCGCCTTACCTAGCAGGTCAAGCAAAGCGGCTTCCAGCGCCGCCACGGCGTTAACGCGTAATTCAAAGGTCCAGGCAc  <  2:299725/139‑1 (MQ=255)                                                                                                                   |                                                                                                                                        CTGTTCATTGCTTTCTGATGCCGCAACTGATACCACTCATGGTTGCCCGGCGTATTTTCCACATAAGGAAGATCGGTTTTGGTCCGATCACCGATATAAAACAGATAACCGAGGACGGTAATAGCCTCGCGTTGCTTGCCTGGCCCTAACAGTTCGCAGACCGGAACATTCAGCGCCTTACCTAGCAGGTCAAGCAAAGCGGCTTCCAGCGCCGCCACGGCGTTAACGCGTAATTCAAAGGTCCAGGCAC  >  NC_000913/2920196‑2920445

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑