Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,067,677	G→A	A135V (GCG→GTG)	yggE ←	oxidative stress defense protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,067,677	0	G	A	100.0%	57.4 / NA	18	A135V (GCG→GTG)	yggE	oxidative stress defense protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/10);  total (8/10)

CGATGAAAGCCGTTCGCCAGTTCCTGCGCCTGATGAATCGCGTTATCAATCGCTGCCTTACGCGCTTTGTCTTTATAGGCATCCGGCTGCGCCACGCCCAGCGACACAGAACGAATTTCGTTAAGACCCGCCTTCAGCGCGCCATCCAGCAAGGAATTCAGTTTGTCTAACTGACGGAGCGTGACTTCCACCGTTCTCACAGCGCGGTAGCCTTTAAGGATACTTTTACCATCCTGATAATCATAATCTGGCTGG  >  NC_000913/3067548‑3067802                                                                                                                                  |                                                                                                                              cGATGAAAGCCGTTCGCCAGTTCCTGCGCCTGATGAATCGCGTTATCAATCGCTGCCTTACGCGCTTTGTCTTTATAGGCATCCGGCTGCGCCACGCCCAGCGACACAGAACGAATTTCGTTAAGACCCACCTTCAgcg                                                                                                                      <  1:365334/139‑1 (MQ=255)             ttCGCCAGTTCCTGCGCCTGATGAATCGCGTTATCAATCGCTGCCTTACGCGCTTTGTCTTTATAGGCATCCGGCTGCGCCACGCCCAGCGACACAGAACGAATTTCGTTAAGACCCACCTTCAGCGCGCCATCCAGCa                                                                                                          >  1:447444/1‑139 (MQ=255)                       ccTGCGCCTGATGAATCGCGTTATCAATCGCTGCCTTACGCGCTTTGTCTTTATAGGCATCCGGCTGCGCCACGCCCAGCGACACAGAACGAATTTCGTTAAGACCCACCTTCAGCGCGCCATCCAGCAAGGAATTCAg                                                                                                <  2:447444/139‑1 (MQ=255)                                gatgaATCGCGTTATCAATCGCTGCCTTACGCGCTTTGTCTTTATAGGCATCCGGCTGCGCCACGCCCAGCGACACAGAACGAATTTCGTTAAGACCCACCTTCAGCGCGCCATCCAGCAAGGAATTCAGTTTGTCTaa                                                                                       >  1:293652/1‑139 (MQ=255)                                 atgaATCGCGTTATCAATCGCTGCCTTACGCGCTTTGTCTTTATAGGCATCCGGCTGCGCCACGCCCAGCGACACAGAACGAATTTCGTTAAGACCCACCTTCAGCGCGCCATCCAGCAAGGAATTCAGTTTGTCTAAc                                                                                      >  1:64367/1‑139 (MQ=255)                                               cAATCGCTGCCTTACGCGCTTTGTCTTTATAGGCATCCGGCTGCGCCACGCCCAGCGACACAGAACGAATTTCGTTAAGACCCACCt                                                                                                                            <  1:67761/87‑1 (MQ=255)                                               cAATCGCTGCCTTACGCGCTTTGTCTTTATAGGCATCCGGCTGCGCCACGCCCAGCGACACAGAACGAATTTCGTTAAGACCCACCt                                                                                                                            >  2:67761/1‑87 (MQ=255)                                                     cTGCCTTACGCGCTTTGTCTTTATAGGCATCCGGCTGTGCCACGCCCAGCGACACAGAACGAATTTCGTTAAGACCCACCTTCAGCGCGCCATCCAGCAAGGAATTCAGTTTGTCTAACTGACGGAGCGTGACTTCCAc                                                                  <  1:286817/139‑1 (MQ=255)                                                        ccTTACGCGCTTTGTCTTTATAGGCATCCGGCTGCGCCACGCCCAGCGACACAGAACGAATTTCGTTAAGACCCACCTTCAGCGCGCCATCCAGCAAGGAATTCAGTTTGTCTAACTGACGGAGCGTGACTTCCACCGt                                                               >  1:266139/1‑139 (MQ=255)                                                                    tGTCTTTATAGGCATCCGGCTGCGCCACGCCCAGCGACACAGAACGAATTTCGTTAAGACCCACCTTCAGCGCGCCATCCAGCAAGGAATTCAGTTTGTCTAACTGACGGAGCGTGACTTCCACCGTTCTCACAGcgcg                                                   <  2:293652/139‑1 (MQ=255)                                                                       cTTTATAGGCATCCGGCTGCGCCACGCCCAGCGACACAGAACGAATTTCGTTAAGACCCACCTTCAGCGCGCCATCCAGCAAGGAATTCAGTTTGTCTAACTGACGGAGCGTGACTTCCACCGTTCTCACAGCGCGGTa                                                <  2:180064/139‑1 (MQ=255)                                                                          tataGGCATCCGGCTGCGCCACGCCCAGCGACACAGAACGAATTTCGTTAAGACCCACCTTCAGCGCGCCATCCAGCAAGGAATTCAGTTTGTCTAACTGACGGAGCGTGACTTCCACCGTTCTCACAGCGCGGTAGcc                                             >  2:2428/1‑139 (MQ=255)                                                                              ggCATCCGGCTGCGCCACGCCCAGCGACACAGAACGAATTTCGTTAAGACCCACCTTCAGCGCGCCATCCAGCAAGGAAt                                                                                                    <  1:173067/80‑1 (MQ=255)                                                                              ggCATCCGGCTGCGCCACGCCCAGCGACACAGAACGAATTTCGTTAAGACCCACCTTCAGCGCGCCATCCAGCAAGGAAt                                                                                                    >  2:173067/1‑80 (MQ=255)                                                                                 aTCCGGCTGCGCCACGCCCAGCGACACAGAACGAATTTCGTTAAGACCCACCTTCAGCGCGCCATCCAGCAAGGAATTCAGttt                                                                                             >  2:199748/1‑84 (MQ=255)                                                                                 aTCCGGCTGCGCCACGCCCAGCGACACAGAACGAATTTCGTTAAGACCCACCTTCAGCGCGCCATCCAGCAAGGAATTCAGttt                                                                                             <  1:199748/84‑1 (MQ=255)                                                                                                         cacaGAACGAATTTCGTTAAGACCCACCTTCAGCGCGCCATCCAGCAAGGAATTCAGTTTGTCTAACTGACGGAGCGTGACTTCCACCGTTCTCACAGCGCGGTAGCCTTTAAGGATACTTTTACCATCCTGataatca              <  1:75070/139‑1 (MQ=255)                                                                                                                     ttCGTTAAGACCCACCTTCAGCGCGCCATCCAGCAAGGAATTCAGTTTGTCTAACTGACGGAGCGTGACTTCCACCGTTCTCACAGCGCGGTAGCCTTTAAGGATACTTTTACCATCCTGATAATCATAATctggctgg  <  1:2428/139‑1 (MQ=255)                                                                                                                                  |                                                                                                                              CGATGAAAGCCGTTCGCCAGTTCCTGCGCCTGATGAATCGCGTTATCAATCGCTGCCTTACGCGCTTTGTCTTTATAGGCATCCGGCTGCGCCACGCCCAGCGACACAGAACGAATTTCGTTAAGACCCGCCTTCAGCGCGCCATCCAGCAAGGAATTCAGTTTGTCTAACTGACGGAGCGTGACTTCCACCGTTCTCACAGCGCGGTAGCCTTTAAGGATACTTTTACCATCCTGATAATCATAATCTGGCTGG  >  NC_000913/3067548‑3067802

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑