Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,081,469	T→C	D56G (GAC→GGC)	tktA ←	transketolase 1, thiamine triphosphate‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,081,469	0	T	C	100.0%	118.6 / NA	32	D56G (GAC→GGC)	tktA	transketolase 1, thiamine triphosphate‑binding
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/13);  total (19/13)

GGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGTTTTTCAGTTCTTCCATCGGCAGATCGTAACCGGTGAGGTGCAGCAGGCTGTAGATCAGCATGGAGCCGTGGCCGTTGGACAGCACGAAGCGGTCACGGTCAGCCCAGGACGGATTCTGCGGGTTGTGTTTCAGGAAATCACGCCACAGGACTTCGGCAATGTCAGCCATACCCATAGGGGCACCCGGGTGACCGGATTTGGCTTTCTGTACTGCGTCCATGCTCAGCGCACGAA  >  NC_000913/3081335‑3081604                                                                                                                                       |                                                                                                                                        ggTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGTTTTTCAGTTCTTCCATCGGCAGATCGTAACCGGTGAGGTGCAGCAGGCTGTAGATCAGCATGGAGCCGTGGCCGTTGGACAGCACGAAGCGGTCACGGCCAGc                                                                                                                                     >  1:256811/1‑139 (MQ=255)           aGTTTTAGAGTGCAGCTGACGGAAGTTTTTCAGTTCTTCCATCGGCAGATCGTAACCGGTGAGGTGCAGCAGGCTGTAGATCAGCATGGAGCCGTGGCCGTTGGACAGCACGAAGCGGTCACGGCCAGCCCAGGACGGa                                                                                                                           <  2:180367/139‑1 (MQ=255)                          cTGACGGAAGTTTTTCAGTTCTTCCATCGGCAGATCGTAACCGGTGAGGTGCAGCAGGCTGTAGATCAGCATGGAGCCGTGGCCGTTGGACAGCACGAAGCGGTCACGGCCAGCCCAGGACGGATTCTGCGGGTTgtgt                                                                                                            >  1:410001/1‑139 (MQ=255)                                      tttCAGTTCTTCCATCGGCAGATCGTAACCGGTGAGGTGCAGCAGGCTGTAGATCAGCATGGAGCCGTGGCCGTTGGACAGCACGAAGCGGTCACGGCCAGCCCAGGACGGATTCTGCGGGTTGTGTTTCAGGAAATCa                                                                                                <  2:323335/139‑1 (MQ=255)                                               ttcCATCGGCAGATCGTAACCGGTGAGGTGCAGCAGGCTGTAGATCAGCATGGAGCCGTGGCCGTTGGACAGCACGAAGCGGTCACGGCCAGCCCAGGACGGATTCTGCGGGTTGTGTTTCAGGAAATCACGCCACAgg                                                                                       >  2:305208/1‑139 (MQ=255)                                                           aTCGTAACCGGTGAGGTGCAGCAGGCTGTAGATCAGCATGGAGCCGTGGCCGTTGGACAGCACGAAGCGGTCACGGCCAGCCCAGGACGGATTCTGCGGGTTGTGTTTCAGGAAATCACGCCACAGGACTTCGGCAATg                                                                           >  2:148792/1‑139 (MQ=255)                                                            tCGTAACCGGTGAGGTGCAGCAGGCTGTAGATCAGCATGGAGCCGTGGCCGTTGGACAGCACGAAGCGGTCACGGCCAGCCCAGGACGGATTCTGCGGGTTGTGTTTCAGGAAATCACGCCACAGGACTTCGGCAATGt                                                                          <  1:148792/139‑1 (MQ=255)                                                            tCGTAACCGGTGAGGTGCAGCAGGCTGTAGATCAGCATGGAGCCGTGGCCGTTGGACAGCACGAAGCGGTCACGGCCAGCCCAGGACGGATTCTGCGGGTTGTGTTTCAGGAAATCACGCCACAGGACTTCGGCAATGt                                                                          <  2:64486/139‑1 (MQ=255)                                                                aaCCGGTGAGGTGCAGCAGGCTGTAGATCAGCATGGAGCCGTGGCCGTTGGACAGCACGAAGCGGTCACGGCCAGCCCAGGACGGATTCt                                                                                                                       <  1:126629/90‑1 (MQ=255)                                                                aaCCGGTGAGGTGCAGCAGGCTGTAGATCAGCATGGAGCCGTGGCCGTTGGACAGCACGAAGCGGTCACGGCCAGCCCAGGACGGATTCt                                                                                                                       >  2:126629/1‑90 (MQ=255)                                                                aaCCGGTGAGGTGCAGCAGGCTGTAGATCAGCATGGAGCCGTGGCCGTTGGACAGCACGAAGCGGTCACGGCCAGCCCAGGACGGATTCTGCGGGTTGTGTTTCAGGAAATCACGCCACAGGACTTCGGCAATGTCAGc                                                                      <  1:305208/139‑1 (MQ=255)                                                                   cGGTGAGGTGCAGCAGGCTGTAGATCAGCATGGAGCCGTGGCCGTTGGACAGCACGAAGCGGTCACGGCCAGCCCAGGACGGATTCTGCGGGTTGTGTTTCAGGAAATCACGCCACAGGACTTCGGCAATGTCAGCCAt                                                                   <  2:376187/139‑1 (MQ=255)                                                                     gTGAGGTGCAGCAGGCTGTAGATCAGCATGGAGCCGTGGCCGTTGGACAGCACGAAGCGGTCACGGCCAGCCCAGGACGGATTCTGCGGGTTGTGTTTCAGGAAATCACGCCACAGGACTTCGGCAATGt                                                                          >  1:393703/1‑130 (MQ=255)                                                                     gTGAGGTGCAGCAGGCTGTAGATCAGCATGGAGCCGTGGCCGTTGGACAGCACGAAGCGGTCACGGCCAGCCCAGGACGGATTCTGCGGGTTGTGTTTCAGGAAATCACGCCACAGGACTTCGGCAATGt                                                                          <  2:393703/130‑1 (MQ=255)                                                                     gTGAGGTGCAGCAGGCTGTAGATCAGCATGGAGCCGTGGCCGTTGGACAGCACGAAGCGGTCACGGCCAGCCCAGGACGGATTCTGCGGGTTGTGTTTCAGGAAATCACGCCACAGGACTTCGGCAATGTCAGCCATAc                                                                 >  2:459441/1‑139 (MQ=255)                                                                     gTGAGGTGCAGCAGGCTGTAGATCAGCATGGAGCCGTGGCCGTTGGACAGCACGAAGCGGTCACGGCCAGCCCAGGACGGATTCTGCGGGTTGTGTTTCAGGAAATCACGCCACAGGACTTCGGCAATGTCAGCCATAc                                                                 >  1:284552/1‑139 (MQ=255)                                                                     gTGAGGTGCAGCAGGCTGTAGATCAGCATGGAGCCGTGGCCGTTGGACAGCACGAAGCGGTCACGGCCAGCCCAGGACGGATTCTGCGGGTTGTGTTTCAGGAAATCACGCCACAGGACTTCGGCAATGTCAGCCATAc                                                                 >  2:148888/1‑139 (MQ=255)                                                                      tgcggTGCAGCAGGCTGTAGATCAGCATGGAGCCGTGGCCGTTGGACAGCACGAAGCGGTCACGGCCAGCCCAGGACGGACTCTGCGGGTTGTGTTTCAGGAAATCACGCCACAGGACTTCGGCAATGTCAGCCATAcc                                                                >  1:147776/4‑139 (MQ=255)                                                                       gAGGTGCAGCAGGCTGTAGATCAGCATGGAGCCGTGGCCGTTGGACAGCACGAAGCGGTCACGGCCAGCCCAGGACGGATTCTGCGGGTTGTGTTTCAGGAAATCACGCCACAGGACTTCGGCAATGTCAGCCATAccc                                                               >  2:288022/1‑139 (MQ=255)                                                                                   gCTGTAGATCAGCATGGAGCCGTGGCCGTTGGACAGCACGAAGCGGTCACGGCCAGCCCAGGACGGATTCTGCGGGTTGTGTTTCAGGAAATCACGCCACAGGACTTCGGCAATGTCAGCCa                                                                    <  1:262216/122‑1 (MQ=255)                                                                                   gCTGTAGATCAGCATGGAGCCGTGGCCGTTGGACAGCACGAAGCGGTCACGGCCAGCCCAGGACGGATTCTGCGGGTTGTGTTTCAGGAAATCACGCCACAGGACTTCGGCAATGTCAGCCa                                                                    >  2:262216/1‑122 (MQ=255)                                                                                     tGTAGATCAGCATGGAGCCGTGGCCGTTGGACAGCACGAAGCGGTCACGGCCAGCCCAGGACGGATTCTGCGGGTTGTGTTTCAGGAAATCACGCCACAGGACTTCGGCAATGTCAGCCATACCCATAGGGGCACCCgg                                                 >  1:328151/1‑139 (MQ=255)                                                                                       tAGATCAGCATGGAGCCGTGGCCGTTGGACAGCACGAAGCGGTCACGGCCAGCCCAGGACGGATTCTGCGGGTTGTGTTTCAGGAAATCACGCCACAGGACTTCGGCAATGTCAGCCATACCCATAGGGGCACCCGGGt                                               >  2:61284/1‑139 (MQ=255)                                                                                          aTCAGCATGGAGCCGTGGCCGTTGGACAGCACGAAGCGGTCACGGCCAGCCCAGGACGGATTCTGCGGGTTGTGTTTCAGGAAATCACGCCACAGGACTTCGGCAATGTCAGCCATACCCATAGGGGCACCCGGGTGAc                                            <  1:148888/139‑1 (MQ=255)                                                                                                 tGGAGCCGTGGCCGTTGGACAGCACGAAGCGGTCACGGCCAGCCCAGGACGGATTCTGCGGGTTGTGTTTCAGGAAATCACGCCACAGGACTTCGGCAATGTCAGCCATACCCATAGGGGCACCCGGGTGACCGGAttt                                     >  1:129823/1‑139 (MQ=255)                                                                                                  ggAGCCGTGGCCGTTGGACAGCACGAAGCGGTCACGGCCAGCCCAGGACGGATTCTGCGGGTTGTGTTTCAGGAAATCACGCCACAGGACTTCGGCAATGTCAGCCATACCCATAGGGGCACCCGGGTGACCGGATTTg                                    <  2:284552/139‑1 (MQ=255)                                                                                                    aGCCGTGGCCGTTGGACAGCACGAAGCGGTCACGGCCAGCCCAGGACGGATTCTGCGGGTTGTGTTTCAGGAAATCACGCCACAGGACTTCGGCAATGTCAGcc                                                                     <  2:494270/104‑1 (MQ=255)                                                                                                    aGCCGTGGCCGTTGGACAGCACGAAGCGGTCACGGCCAGCCCAGGACGGATTCTGCGGGTTGTGTTTCAGGAAATCACGCCACAGGACTTCGGCAATGTCAGcc                                                                     >  1:494270/1‑104 (MQ=255)                                                                                                    aGCCGTGGCCGTTGGACAGCACGAAGCGGTCACGGCCAGCCCAGGACGGATTCTGCGGGTTGTGTTTCAGGAAATCACGCCACAGGACTTCGGCAATGTCAGCCATACCCATAGGGGCACCCGGGTGACCGGATTTGGc                                  >  2:85743/1‑139 (MQ=255)                                                                                                         tGGCCGTTGGACAGCACGAAGCGGTCACGGCCAGCCCAGGACGGATTCTGCGGGTTGTGTTTCAGGAAATCACGCCACAGGACTTCGGCAATGTCAGCCATACCCATAGGGGCACCCGGGTGACCGGATTTGGCTTTCt                             >  1:341682/1‑139 (MQ=255)                                                                                                         tGGCCGTTGGACAGCACGAAGCGGTCACGGCCAGCCCAGGACGGATTCTGCGGGTTGTGTTTCAGGAAATCACGCCACAGGACTTCGGCAATGTCAGCCATACCCATAGGGGCACCCGGGTGACCGGATTTGGCTTTCt                             >  1:194063/1‑139 (MQ=255)                                                                                                             cGTTGGACAGCACGAAGCGGTCACGGCCAGCCCAGGACGGATTCTGCGGGTTGTGTTTCAGGAAATCACGCCACAGGACTTCGGCAATGTCAGCCATACCCATAGGGGCACCCGGGTGACCGGATTTGGCTTTCTGTAc                         <  1:107055/139‑1 (MQ=255)                                                                                                                         cGAAGCGGTCACGGCCAGCCCAGGACGGATTCTGCGGGTTGTGTTTCAGGAAATCACGCCACAGGACTTCGGCAATGTCAGCCATACCCATAGGGGCACCCGGGTGACCGGATTTGGCTTTCTGTACTGCGTCCATGCt             <  1:288022/139‑1 (MQ=255)                                                                                                                               ggtcacggccaGCCCAGGACGGATTCTGCGGGTTGTGTTTCAGGAAATCACGCCACAGGACTTCGGCAATGTCAGCCATACCCATAGGGGCACCCGGGTGACCGGATTTGGCTTTCTGTACTGCGTCCATGCTCAgcgc       <  1:233775/139‑1 (MQ=255)                                                                                                                                    cggccaGCCCAGGACGGATTCTGCGGGTTGTGTTTCAGGAAATCACGCCACAGGACTTCGGCAATGTCAGCCATACCCATAGGGGCACCCGGGTGACCGGATTTGGCTTTCTGTACTGCGTCCATGCTCAGCGCACGaa  <  2:341682/139‑1 (MQ=255)                                                                                                                                       |                                                                                                                                        GGTGACCCGGAGTTTTAGAGTGCAGCTGACGGAAGTTTTTCAGTTCTTCCATCGGCAGATCGTAACCGGTGAGGTGCAGCAGGCTGTAGATCAGCATGGAGCCGTGGCCGTTGGACAGCACGAAGCGGTCACGGTCAGCCCAGGACGGATTCTGCGGGTTGTGTTTCAGGAAATCACGCCACAGGACTTCGGCAATGTCAGCCATACCCATAGGGGCACCCGGGTGACCGGATTTGGCTTTCTGTACTGCGTCCATGCTCAGCGCACGAA  >  NC_000913/3081335‑3081604

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑