Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,120,324	A→G	V319A (GTG→GCG)	glcA ←	glycolate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,120,324	0	A	G	100.0%	98.5 / NA	26	V319A (GTG→GCG)	glcA	glycolate transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/13);  total (13/13)

GGCGACAATGGGTGCCGCTTTCAACACTTGTTGATGCAAATGAGGGATCTGGAAATTAATCACCAGTGAATAAAACGCGCCGCCCGGAGCAAATAACGCTTTAAACGGCTTCATGGTCCAGATGGTGACCAGCACCGTTAAGATTAAAAACGGTGACCACGCTCGAATGATTTGCCCCAGACTATATTCTGAAGGCACGGGACCGCCAGAAGATGGCTTATTTACCACCATCGCACCTGCGGATTGTCCCATGCTGATTG  >  NC_000913/3120191‑3120450                                                                                                                                      |                                                                                                                               ggCGACAATGGGTGCCGCTTTCAACACTTGTTGATGCAAATGAGGGATCTGGAAATTAATCACCAGTGAATAAAACGCGCCGCCCGGAGCAAATAACGCTTTAAACGGCTTCATGGTCCAGATGGTGACCAGCGCCGtt                                                                                                                           >  2:272307/1‑139 (MQ=255) ggCGACAATGGGTGCCGCTTTCAACACTTGTTGATGCAAATGAGGGATCTGGAAATTAATCACCAGTGAATAAAACGCGCCGCCCGGAGCAAATAACGCTTTAAACGGCTTCATGGTCCAGATGGTGACCAGCGCCGtt                                                                                                                           <  2:2644/139‑1 (MQ=255)                                  aTGCAAATGAGGGATCTGGAAATTAATCACCAGTGAATAAAACGCGCCGCCCGGAGCAAATAACGCTTTAAACGGCTTCATGGTCCAGATGGTGACCAGCGCCGTTAAGATTAAAAACGGTGACCACGCTCGAATGAtt                                                                                          <  1:272307/139‑1 (MQ=255)                                      aaaTGAGGGATCTGGAAATTAATCACCAGTGAATAAAACGCGCCGCCCGGAGCAAATAACGCTTTAAACGGCTTCATGGTCCAGATGGTGACCAGCGCCGTTAAGATTAAAAACGGTGACCACGCTCGAATGATTTGcc                                                                                      <  1:285031/139‑1 (MQ=255)                                        aTGAGGGATCTGGAAATTAATCACCAGTGAATAAAACGCGCCGCCCGGAGCAAATAACGCTTTAAACGGCTTCATGGTCCAGATGGTGACCAGCGCCGTTAAGATTAAAAACGGTGACCACGCTCGAATGATTTGcccc                                                                                    <  1:172455/139‑1 (MQ=255)                                        aTGAGGGATCTGGAAATTAATCACCAGTGAATAAAACGCGCCGCCCGGAGCAAATAACGCTTTAAACGGCTTCATGGTCCAGATGGTGACCAGCGCCGTTAAGATTAAAAACGGTGACCACGCTCGAATGATTTGcccc                                                                                    <  2:226594/139‑1 (MQ=255)                                           aGGGATCTGGAAATTAATCACCAGTGAATAAAACGCGCCGCCCGGAGCAAATAACGCTTTAAACGGCTTCATGGTCCAGATGGTGACCAGCGCCGTTAAGATTAAAAACGGTGACCACGCTCGAATGATTTGCCCCAGa                                                                                 <  2:111341/139‑1 (MQ=255)                                                  tGGAAATTAATCACCAGTGAATAAAACGCGCCGCCCGGAGCAAATAACGCTTTAAACGGCTTCATGGTCCAGATGGTGACCAGCGCCGTTAAGATTAAAAACGGTGACCACGCTCGAATGATTTGccc                                                                                     >  1:110058/1‑128 (MQ=255)                                                  tGGAAATTAATCACCAGTGAATAAAACGCGCCGCCCGGAGCAAATAACGCTTTAAACGGCTTCATGGTCCAGATGGTGACCAGCGCCGTTAAGATTAAAAACGGTGACCACGCTCGAATGATTTGccc                                                                                     <  2:110058/128‑1 (MQ=255)                                                  tGGAAATTAATCACCAGTGAATAAAACGCGCCGCCCGGAGCAAATAACGCTTTAAACGGCTTCATGGTCCAGATGGTGACCAGCGCCGTTAAGATTAAAAACGGTGACCACGCTCGAATGATTTGCCCCAGACTATAtt                                                                          >  1:131163/1‑139 (MQ=255)                                                                  gTGAATAAAACGCGCCGCCCGGAGCAAATAACGCTTTAAACGGCTTCATGGTCCAGATGGTGACCAGCGCCGTTAAGATTAAAAACGGTGACCACGCTCGAATGATTTGCCCCAGACTATATTCTGAAGGCACGGGAcc                                                          >  1:14860/1‑139 (MQ=255)                                                                  gTGAATAAAACGCGCCGCCCGGAGCAAATAACGCTTTAAACGGCTTCATGGTCCAGATGGTGACCAGCGCCGTTAAGATTAAAAACGGTGACCACGCTCGAATGATTTGCCCCAGACTATATTCTGAAGGCACGGGAcc                                                          >  1:423069/1‑139 (MQ=255)                                                                             gcgcCGCCCGGAGCAAATAACGCTTTAAACGGCTTCATGGTCCAGATGGTGACCAGCGCCGTTAAGATTAAAAACGGTGACCACGCTCGAATGAtt                                                                                          <  2:315830/96‑1 (MQ=255)                                                                             gcgcCGCCCGGAGCAAATAACGCTTTAAACGGCTTCATGGTCCAGATGGTGACCAGCGCCGTTAAGATTAAAAACGGTGACCACGCTCGAATGAtt                                                                                          >  1:315830/1‑96 (MQ=255)                                                                             gcgcCGCCCGGAGCAAATAACGCTTTAAACGGCTTCATGGTCCAGATGGTGACCAGCGCCGTTAAGATTAAAAACGGTGACCACGCTCGAATGATTTGCCCCAGACTATATTCTGAAGGCACGGGACCGCCAGAAGATg                                               >  2:435809/1‑139 (MQ=255)                                                                               gccgccCGGAGCAAATAACGCTTTAAACGGCTTCATGGTCCAGATGGTGACCAGCGCCGtt                                                                                                                           >  1:352639/1‑61 (MQ=255)                                                                               gccgccCGGAGCAAATAACGCTTTAAACGGCTTCATGGTCCAGATGGTGACCAGCGCCGtt                                                                                                                           <  2:352639/61‑1 (MQ=255)                                                                                               aaCGCTTTAAACGGCTTCATGGTCCAGATGGTGACCAGCGCCGTTAAGATTAAAAACGGTGACCACGCTCGAATGATTTGCCCCAGACTATATTCTGAAGGCACGGGACCGCCAGAAGATGGCTTATTTACCACCATCg                             <  2:110249/139‑1 (MQ=255)                                                                                                  gCTTTAAACGGCTTCATGGTCCAGATGGTGACCAGCGCCGTTAAGATTAAAAACGGTGACCACGCTCGAATGATTTGcc                                                                                      >  2:289913/1‑79 (MQ=255)                                                                                                  gCTTTAAACGGCTTCATGGTCCAGATGGTGACCAGCGCCGTTAAGATTAAAAACGGTGACCACGCTCGAATGATTTGcc                                                                                      <  1:289913/79‑1 (MQ=255)                                                                                                       aaaCGGCTTCATGGTCCAGATGGTGACCAGCGCCGTTAAGATTAAAAACGGTGACCACGCTCGAATGATTTGCCCCAGACTATATTCTGAAGGCACGGGACCGCCAGAAGATGGCTTATTTACCACCATCGCACCTGCg                     >  2:344759/1‑139 (MQ=255)                                                                                                                     tCCAGATGGTGACCAGCGCCGTTAAGATTAAAAACGGTGACCACGCTCGAATGATTTGCCCCAGACTATATTCTGAAGGCACGGGACCGCCAGAAGATGGCTTATTTACCACCATCGCACCTGCGGATTGTCCCATGCt       >  2:128252/1‑139 (MQ=255)                                                                                                                         gATGGTGACCAGCGCCGTTAAGATTAAAAACGGTGACCACGCTCGAATGATTTGCCCCAGACTATATTCTGAAGGCACGGGACCGCCAGAAGATGGCTTATTTACCACCATCGCACCTGCGGATTGTCCCATGCTGAtt   >  1:298891/1‑139 (MQ=255)                                                                                                                          aTGGTGACCAGCGCCGTTAAGATTAAAAACGGTGACCACGCTCGAATGATTTGCCCCAGACTATATTCTGAAGGCACGGGACCGCCAGAAGATGGCTTATTTACCACCATCGCACCTGCGGATTGTCCCATGCTGATTg  <  2:201660/139‑1 (MQ=255)                                                                                                                               gACCAGCGCCGTTAAGATTAAAAACGGTGACCACGCTCGAATGATTTGTCCCAGACTATATTCTGAAGGCACGGGACCGCCAGAAGATGGCTTAt                                         >  2:24893/1‑95 (MQ=255)                                                                                                                               gACCAGCGCCGTTAAGATTAAAAACGGTGACCACGCTCGAATGATTTGTCCCAGACTATATTCTGAAGGCACGGGACCGCCAGAAGATGGCTTAt                                         <  1:24893/95‑1 (MQ=255)                                                                                                                                      |                                                                                                                               GGCGACAATGGGTGCCGCTTTCAACACTTGTTGATGCAAATGAGGGATCTGGAAATTAATCACCAGTGAATAAAACGCGCCGCCCGGAGCAAATAACGCTTTAAACGGCTTCATGGTCCAGATGGTGACCAGCACCGTTAAGATTAAAAACGGTGACCACGCTCGAATGATTTGCCCCAGACTATATTCTGAAGGCACGGGACCGCCAGAAGATGGCTTATTTACCACCATCGCACCTGCGGATTGTCCCATGCTGATTG  >  NC_000913/3120191‑3120450

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑