Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,269,600	T→C	*396Q (TAG→CAG)	yhaC →	pentapetide repeats‑related protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,269,600	0	T	C	100.0%	130.4 / NA	34	*396Q (TAG→CAG)	yhaC	pentapetide repeats‑related protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/16);  total (18/16)

CTCGAGAAAGACGAAAATTCTCCGGACAATGGCTTTGAGGTCGGGGAATATAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAACCGCAAAAATGCCT  >  NC_000913/3269465‑3269725                                                                                                                                        |                                                                                                                              cTCGAGAAAGACGAAAATTCTCCGGACAATGGCTTTGAGGTCGGGGAATATAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATACAGa                                                                                                                            >  1:400267/1‑139 (MQ=255)   cGAGAAAGACGAAAATTCTCCGGACAATGGCTTTGAGGTCGGGGAATATAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATACAGACt                                                                                                                          >  2:736/1‑139 (MQ=255)                    ctcCGGACAATGGCTTTGAGGTCGGGGAATATAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATACAGACTTAATAACTTCTTACaaa                                                                                                         <  2:191934/139‑1 (MQ=255)                      ccGGACAATGGCTTTGAGGTCGGGGAATATAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATACAGACTTAATAACTTCTTACAAAGt                                                                                                       <  1:404445/139‑1 (MQ=255)                        ggACAATGGCTTTGAGGTCGGGGAATATAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATACAGACTTAATAACTTCTTACAAAGTCt                                                                                                     <  2:88531/139‑1 (MQ=255)                         gACAATGGCTTTGAGGTCGGGGAATATAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATACAGACTTAATAACTTCTTACAAAGTCta                                                                                                    <  2:131071/139‑1 (MQ=255)                                       ggTCGGGGAATATAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATACAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTa                                                                                      <  1:305127/139‑1 (MQ=255)                                                atatAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATACAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGAtatttat                                                                             <  2:388855/139‑1 (MQ=255)                                                atatAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATACAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGAtatttat                                                                             <  2:327842/139‑1 (MQ=255)                                                          tatGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATACAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTAcc                                                                   <  1:426212/139‑1 (MQ=255)                                                           atGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATACAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTa                                                                     >  1:504294/1‑136 (MQ=255)                                                           atGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATACAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTa                                                                     <  1:297746/136‑1 (MQ=255)                                                           atGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATACAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTa                                                                     <  2:504294/136‑1 (MQ=255)                                                           atGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATACAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTa                                                                     >  2:297746/1‑136 (MQ=255)                                                                gCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATACAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGc                                                             >  2:29944/1‑139 (MQ=255)                                                                     taACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATACAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGAtatttat                                                                             <  2:52176/118‑1 (MQ=255)                                                                     taACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATACAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGAtatttat                                                                             >  1:52176/1‑118 (MQ=255)                                                                        cTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATACAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAg                                                     >  2:169005/1‑139 (MQ=255)                                                                             agaAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATACAGACTTAATAACTTCTTACaaa                                                                                                         >  1:22214/1‑82 (MQ=255)                                                                             agaAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATACAGACTTAATAACTTCTTACaaa                                                                                                         <  2:22214/82‑1 (MQ=255)                                                                                aGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATACAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTAcc                                                                   <  1:31879/117‑1 (MQ=255)                                                                                aGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATACAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTAcc                                                                   >  2:31879/1‑117 (MQ=255)                                                                                  cAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATACAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCa                                           >  2:87228/1‑139 (MQ=255)                                                                                           aCCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATACAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCgg                                  <  1:29944/139‑1 (MQ=255)                                                                                                            gTATGATTACGACCTTTTAAATGCAATACAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGAcgcg                                   >  2:485270/1‑121 (MQ=255)                                                                                                            gTATGATTACGACCTTTTAAATGCAATACAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGAcgcg                                   <  1:485270/121‑1 (MQ=255)                                                                                                              aTGATTACGACCTTTTAAATGCAATACAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAAc               >  2:136199/1‑139 (MQ=255)                                                                                                              aTGATTACGACCTTTTAAATGCAATACAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAAc               >  2:441202/1‑139 (MQ=255)                                                                                                                 aTTACGACCTTTTAAATGCAATACAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGAtatttat                                                                             <  2:219301/74‑1 (MQ=255)                                                                                                                 aTTACGACCTTTTAAATGCAATACAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGAtatttat                                                                             >  1:219301/1‑74 (MQ=255)                                                                                                                 aTTACGACCTTTTAAATGCAATACAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAACCGc            >  1:397689/1‑139 (MQ=255)                                                                                                                   tACGACCTTTTAAATGCAATACAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAACCGCaa          >  1:358629/1‑139 (MQ=255)                                                                                                                   tACGACCTTTTAAATGCAATACAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAACCGCaa          >  1:268955/1‑139 (MQ=255)                                                                                                                           tttAAATGCAATACAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAACCGCAAAAATGCCt  >  1:178794/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                              CTCGAGAAAGACGAAAATTCTCCGGACAATGGCTTTGAGGTCGGGGAATATAACACATATGAAGCATATAACTCAGAGAAGCAATATTTTACCAGAGAGGACTATACGTATGATTACGACCTTTTAAATGCAATATAGACTTAATAACTTCTTACAAAGTCTATTATTAATACCGTAGATATTTATTCATCTTACCCTGTGCACCAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAACCGCAAAAATGCCT  >  NC_000913/3269465‑3269725

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑